RU2562590C1 - METHOD FOR ENHANCING ANTIBIOTIC SUSCEPTIBILITY OF OPPORTUNISTIC-PATHOGENIC MICROFLORA BY ADDING LACTIC ACID FEED SUPPLEMENT CONTAINING CULTURE Bifidobacter longum "Б-41" in vitro - Google Patents
METHOD FOR ENHANCING ANTIBIOTIC SUSCEPTIBILITY OF OPPORTUNISTIC-PATHOGENIC MICROFLORA BY ADDING LACTIC ACID FEED SUPPLEMENT CONTAINING CULTURE Bifidobacter longum "Б-41" in vitro Download PDFInfo
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Изобретение относится к ветеринарии и может быть использовано в лабораторной работе при изучении антибиотикочувствительности микроорганизмов.The invention relates to veterinary medicine and can be used in laboratory work in the study of antibiotic sensitivity of microorganisms.
Наиболее близким к заявленному решению известен «Способ подавления микрофлоры» (патент РФ 2053773 кл. А61K 31/71, А61K 33/38 от 10.02.1996), заключающийся в том, повышение чувствительности достигается за счет применения антибиотиков одновременно или последовательно с электролизным серебром в концентрации 2,5-5,0% и физиологическим раствором (0,89% NaCl).Closest to the claimed solution is known "Method of suppressing microflora" (RF patent 2053773 class A61K 31/71, A61K 33/38 from 02.10.1996), which consists in increasing the sensitivity through the use of antibiotics simultaneously or sequentially with electrolysis silver in concentration of 2.5-5.0% and physiological saline (0.89% NaCl).
К недостаткам известного способа можно отнести отсутствие данных по росту антибиотикочувствительности к конкретному виду антибактериального вещества, а также отсутствие данных по росту показателей зон задержки роста тест-культуры до и после контакта с испытуемым препаратом.The disadvantages of this method include the lack of data on the growth of antibiotic sensitivity to a specific type of antibacterial substance, as well as the lack of data on the growth of indicators of growth inhibition zones of the test culture before and after contact with the test drug.
Задача изобретения - расширение арсенала способов повышения антибиотикочувствительности микроорганизмов in vitro.The objective of the invention is the expansion of the arsenal of methods for increasing the antibiotic sensitivity of microorganisms in vitro.
Указанная задача достигается тем, что в способе исследования повышения антибиотикочувствительности условно-патогенной микрофлоры при использовании молочнокислой кормовой добавки, содержащей культуру микроорганизмов Bifidobacter longum Б-41 in vitro, включающем добавление к 0,5 мл мясопептонного бульона (МПБ) 0,5 мл супернатанта молочно-кислой комовой добавки, содержащей культуру микроорганизмов Bifidobacter longum Б-41, полученного в разные сроки роста на 1, 10, 20 и 30 день, после чего вносят 0,5 мл МПБ суточной культуры одного из штаммов Е. fecalis 200, St. albus АТСС 25923, S. enteritidis 182, E. coli ATCC 25222, Pr. vulgaris 192, Kl. pneumonia 71 условно-патогенной микрофлоры, полученный раствор инкубируют 18-24 ч при Т=37,0±0,5°C, после чего проводят определение антибиотикочувствительности микроорганизмов на мясопептонном агаре с 24 видами антибактериальных препаратов дискодиффузионным методом.This task is achieved by the fact that in the method of studying the increase in antibiotic sensitivity of opportunistic microflora when using a lactic acid feed supplement containing a culture of Bifidobacter longum B-41 microorganisms in vitro, which includes adding 0.5 ml of milk supernatant to 0.5 ml meat-peptone broth (MPB) acid lumpy supplement containing a culture of microorganisms Bifidobacter longum B-41, obtained at different growth times on 1, 10, 20 and 30 days, after which 0.5 ml of MPB daily culture of one of the strains of E. fecalis 200, St. albus ATCC 25923, S. enteritidis 182, E. coli ATCC 25222, Pr. vulgaris 192, Kl. pneumonia 71 of conditionally pathogenic microflora, the resulting solution is incubated for 18-24 hours at T = 37.0 ± 0.5 ° C, after which the antibiotic sensitivity of the microorganisms is determined on meat and peptone agar with 24 types of antibacterial drugs by the disk diffusion method.
Пример 1. Анализ изменения зоны задержки роста микроорганизмов при определении антибиотикочувствительности после контакта с молочно-кислой кормовой добавкой, содержащей культуру микроорганизмов разных сроков культивирования, показал разную чувствительность у исследуемых представителей условно-патогенной микрофлоры. Так, наибольший рост антибиотикочувствительности микроорганизмов отмечается после культивирования с десяти- и тридцатидневными культурами (табл. 1).Example 1. Analysis of changes in the zone of growth inhibition of microorganisms in determining antibiotic sensitivity after contact with a lactic acid feed supplement containing a culture of microorganisms of different cultivation periods showed different sensitivity in the studied representatives of conditionally pathogenic microflora. Thus, the greatest increase in the antibiotic sensitivity of microorganisms is observed after cultivation with ten- and thirty-day cultures (Table 1).
Наибольшее увеличение антибиотикочувствительности (на 89,2%) отмечено у Е. fecalis 200 после контакта с десятидневной молочно-кислой кормовой добавкой, содержащей культуру микроорганизмов Bifidobacter longum Б-41.The greatest increase in antibiotic sensitivity (by 89.2%) was observed in E. fecalis 200 after contact with a ten-day lactic acid feed supplement containing a microorganism culture of Bifidobacter longum B-41.
Пример 2 Установлено, что на протяжении всего эксперимента супернатант молочнокислой кормовой добавки, содержащей культуру микроорганизмов Bifidobacter longum Б-41 разного срока культивирования, вызывает устойчивый рост количества антибактериальных препаратов, к которым чувствительны изучаемые штаммы условно-патогенной микрофлоры. Кроме того, увеличение срока инкубирования пробиотика совпадает со снижением количества малочувствительных препаратов у штаммов условно-патогенной микрофлоры (табл. 2).Example 2 It was found that throughout the experiment, the supernatant of a lactic acid feed additive containing a culture of microorganisms Bifidobacter longum B-41 with different cultivation periods causes a steady increase in the number of antibacterial drugs to which the studied strains of conditionally pathogenic microflora are sensitive. In addition, an increase in the probiotic incubation period coincides with a decrease in the number of low-sensitivity preparations in opportunistic microflora strains (Table 2).
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Cited By (3)
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RU2646799C2 (en) * | 2016-05-26 | 2018-03-07 | Александр Николаевич Швыдков | Method for investigation of increased antibiotic sensitivity of pathogenic and conditionally pathogenic microflora in vitro by lactic feed additive containing propionobacterium freundenreichii shermanii ac-103 microorganisms culture |
RU2647761C2 (en) * | 2016-02-24 | 2018-03-20 | Александр Николаевич Швыдков | Method for investigation of increased antibiotic sensitivity of conditionally pathogenic microflora in vitro by lactic feed additive containing lactobacillus acidophilus la-5 microorganisms culture |
RU2678447C2 (en) * | 2017-02-01 | 2019-01-29 | Александр Николаевич Швыдков | Method for researching increase of antibiotic sensitivity of conditionally-pathogenic microflora in vitro with milk-foundation fodder additive containing streptococcus termophilus th-4 microorganism culture |
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RU2045576C1 (en) * | 1991-05-06 | 1995-10-10 | Щедрин Виталий Иванович | Method for determining sensitivity of plague inducer to antibiotics |
RU2500411C2 (en) * | 2012-08-24 | 2013-12-10 | Федеральное государственное бюджетное учреждение науки Институт клеточного и внутриклеточного симбиоза Уральского отделения Российской академии наук | Method for individual selection of preparations containing probiotic lactic bacterial strains for effective intravaginal therapy |
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RU2045576C1 (en) * | 1991-05-06 | 1995-10-10 | Щедрин Виталий Иванович | Method for determining sensitivity of plague inducer to antibiotics |
RU2500411C2 (en) * | 2012-08-24 | 2013-12-10 | Федеральное государственное бюджетное учреждение науки Институт клеточного и внутриклеточного симбиоза Уральского отделения Российской академии наук | Method for individual selection of preparations containing probiotic lactic bacterial strains for effective intravaginal therapy |
Non-Patent Citations (1)
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Определение чувствительности микроорганизмов к антибактериальным препаратам. Перечень данных [он-лайн] 04.03.2004 [найдено 2014.09.27] - найдено в Интернете: URL: http://www.fcgsen.ru/DOC/lab_doc/muk_1890-04.pdf. * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2647761C2 (en) * | 2016-02-24 | 2018-03-20 | Александр Николаевич Швыдков | Method for investigation of increased antibiotic sensitivity of conditionally pathogenic microflora in vitro by lactic feed additive containing lactobacillus acidophilus la-5 microorganisms culture |
RU2646799C2 (en) * | 2016-05-26 | 2018-03-07 | Александр Николаевич Швыдков | Method for investigation of increased antibiotic sensitivity of pathogenic and conditionally pathogenic microflora in vitro by lactic feed additive containing propionobacterium freundenreichii shermanii ac-103 microorganisms culture |
RU2678447C2 (en) * | 2017-02-01 | 2019-01-29 | Александр Николаевич Швыдков | Method for researching increase of antibiotic sensitivity of conditionally-pathogenic microflora in vitro with milk-foundation fodder additive containing streptococcus termophilus th-4 microorganism culture |
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