RU2538086C2 - Method of treating diabetes mellitus, complicated by accompanying diseases - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention relates to medicine, namely to endocrinology, and deals with treatment of diabetes mellitus, complicated by accompanying diseases, in particular, by parasite invasions. For this purpose electro-activated water solutions of inorganic salts are introduced at the background of antihyperglycemic medications. On first 5 days solution of anolyte (EWS-A) with redox potential (RP) from +700 to +800 mV and with pH from 6.0 to 4.5, after which for 5 days introduced is catholyte solution (EWS-C) with RP from -400 to -600 mV and pH from 9.5 to 10.5; then for 10 days introduced are heated to +36°C - +37°C EWS-C and EWS-A are introduced with their alternation for 24 hours. In case of accompanying parasite invasion, EWS-A in combination with anti-parasitic vegetable medications in form of rectal microclysters for first 5 days of treatment is additionally introduced for first 5 days of treatment. EWS-C in combination with rectal microclysters with probiotic additives is introduced for the following 10 days. In case of detection of Opisthorchida, biltricide is introduced on the 15-th day in dose 75 mg/kg, and duodenal probing with anti-parasitic medication is carried out on the 16-th day. Starting with the 6-th day and to the completion of treatment (21-st day) fermented milk product "Narine" is administered in dose 1 tablespoon 3 times per day 30-40 minutes before meal.EFFECT: mode of introducing electro-activated water solutions in complex with probiotics and anti-parasitic preparations provides efficient treatment of parasite invasions in combination with gradual normalisation of carbohydrate and lipid exchange in patients with diabetes mellitus.2 cl, 2 ex, 4 tbl

Description

The invention relates to medicine, namely to means for regulating blood sugar in patients with type 2 diabetes, complicated by a number of concomitant diseases, and involves the use of electroactivated aqueous solutions of inorganic salts (EVR-K and EVR-A) with therapeutic properties.

Type 2 diabetes mellitus occurs when there is a violation of the absorption of insulin in the tissues. In this case, insulin is produced in normal or even increased amounts, but cellular receptors do not perceive it. This condition is called insulin resistance. The pancreas begins to produce more and more insulin in order to activate cell transport systems to absorb glucose circulating in the blood. After some time, beta-cell depletion occurs, and insulin production decreases.

This form of diabetes is also called non-insulin-dependent, since the correction of plasma glucose in the first stages of the disease does not require the introduction of insulin. Traditionally, at the beginning of the disease, a diet, dosed physical activity and tablet preparations are used that slow down the absorption of glucose in the gastrointestinal tract, increase insulin release by pancreatic cells and / or and enhance glucose uptake by cells.

Some other diseases, including systemic diseases, manifested in different clinical forms, can contribute to the development of diabetes. In the set of diseases associated with diabetes, parasitic ones, such as opisthorchiasis, which are characterized by signs of damage to the parasitic organs by helminths (liver, bile ducts, gall bladder, pancreas) and changes in the gastrointestinal tract (dysbacteriosis), take not the last place. , intestinal dysfunctions, enterocolitis, intestinal infections, etc.).

A large number of parasites, such as, for example, trematode, affecting the bile ducts and pancreas, can cause pancreatitis and cirrhosis. In some patients, there is a violation of the incremental function of the pancreas, leading to fasting hyperglycemia.

All this leads to the conclusion: diabetes can be the result of damage to the pancreas by parasites. Their presence leads to the destruction of the gland tissue, in addition, they absorb the trace elements necessary for the normal functioning and production of hormones: chromium, zinc, selenium, manganese, etc.

The most common disease that accompanies diabetes is opisthorchiasis - a disease caused by opisthorchis - helminths from the flukes class. The disease is characterized by a chronic course with damage to the liver, pancreas, bile ducts.

Opisthorchisis are detected in the intrahepatic, bile ducts in 100% of invaded people, in the gall bladder - in 60%, in the pancreas - in 36%. Metacercaria, which penetrated the hepatobiliary system and pancreas, reach puberty after 3-4 weeks and begin to lay eggs. Thus, the full cycle of development of the pathogen opisthorchiasis (from an egg to a mature parasite) lasts 4-4.5 months, after which egg production begins. The lifespan of opisthorchis reaches 20-25 years. The most secluded place of accumulation of opisthorchus is the pancreatic ducts. The complexity of the treatment of such diseases is that it is not easy to diagnose them, and even conducting appropriate tests does not always give a reliable result.

Most researchers agree that one of the most likely causes of diabetes is precisely parasitosis. Reproduction of parasites leads to the fact that cells do not receive the necessary trace elements, such as selenium, zinc, chromium and manganese, in addition, the content of heavy metals in the body increases.

The liver is a natural barrier that protects the body from xenobiotics. Impaired liver function leads to the fact that its depotting ability and the ability to filter blood deteriorates, cleansing it of small worm larvae. In addition, the liver loses its ability to remove propylene alcohol from the body, which is formed in the body when using cosmetics and detergents, the use of carbonated drinks, tea, instant coffee. All this leads to a violation of the homeostasis of the body, a decrease in the activity of sugar binding processes in the liver and muscles, and destruction of the functional tissue of the pancreas, which is noted as “diffuse changes” during ultrasound examination. A special place among the states of ill health associated with intestinal dysfunctions is occupied by dysbiosis that occurs during treatment with antibiotics and immunosuppressants or under the influence of harmful environmental factors.

Diabetes mellitus, as a rule, is associated with impaired immune systems. Imbalance in the microflora, and then the immune status is an impetus that determines the development and course of the underlying disease. In this regard, the scheme of a comprehensive phased rehabilitation of diabetes should include funds that stimulate the reactivity of the body, including by restoring the normal microbial flora of the colon.

Type 2 diabetes (non-insulin-dependent) often develops against the background of obesity. In this type of diabetes, insulin production is not impaired, but due to obesity, insulin sensitivity decreases, resulting in an increase in blood sugar. A major role in the violation of carbohydrate metabolism is played by the insufficiency of trace elements - manganese, copper, potassium and calcium salts, which are involved in the synthesis of insulin, contribute to lowering sugar and fat in the blood and the absorption of carbohydrates in tissues.

In order to slow down the absorption of glucose in the gastrointestinal tract, increase the release of insulin by the cells of the pancreas and suppress acute infection, drugs are used that have a hypoglycemic effect, stimulate the production of insulin, regulate the function of the digestive glands, lower blood fats and prevent various complications of diabetes.

The authors of this development propose to prescribe these drugs to patients in combination with electroactivated aqueous solutions of inorganic salts, which have the ability to enhance the generation of free radicals that have a biocidal effect that is superior to many traditional antibacterial and disinfectants in their antimicrobial activity.

As electroactivated aqueous solutions of inorganic salts, an AgNO ₃ salt in a concentration of not more than 7 g / l or a mixture of NaCl, KCl, CaCl ₂ , MgCl ₂ salts in the same proportions as in human blood plasma with a total salt concentration of not more than 10 are used g / l Electroactivated aqueous solutions of inorganic salts from the group consisting of AgNO ₃ , NaCl, KCl, CaCl ₂ and MgSO ₄ are introduced into the body with a negative redox potential (catholyte) or with a positive redox potential (anolyte).

It is known to use electroactivated aqueous solutions of inorganic salts in the treatment of concomitant skin diseases (pemphigus) specific for diabetes mellitus, especially those associated with diabetic neuropathy, as well as bacterial skin infections, fungal diseases, which are especially dangerous for patients with diabetes mellitus. Some skin conditions, such as lipoid necrobiosis in diabetes, indicate a close association with diabetes. Electro-activated aqueous solutions saturated with microelements are effective in the treatment of type 1 and type 2 diabetes and reduce the need for insulin in patients by 20-30% (Ashbah D. Living and dead water - the latest medicine of our time. M., 2008).

One of the most important parameters of water is its redox potential (redox potential). The metabolic processes in the cell are carried out through its membrane, which has an electrical potential of approximately -70mV. To activate or open the membrane channels and carry out metabolism, a slightly prevailing electrical potential must exist in the intercellular space, which recharges the membrane - a change in potential between the inner and outer sides of the membrane, which opens the gate to the cell. The exchange of water in the body is one of the most voluminous processes. Due to its need for life, water as a priority liquid acquires a sufficient electric charge. If water does not bring with it a charge of at least -70mV when it enters the body, then it makes up for its deficiency at the expense of other carriers, depleting energy reserves for other important processes. Such a violation of the process leads to premature aging and the occurrence of any possible pathology. Therefore, water for oral administration must be saturated with a sufficient number of electrons.

As a result of the cathodic treatment, water (catholyte) acquires an alkaline reaction, its ORP decreases, the surface tension decreases, the amount of dissolved oxygen and nitrogen decreases, the concentration of hydrogen, free hydroxyl groups increases, the electrical conductivity decreases, and the structure of not only ion hydration shells, but also free volume water (Bakhir V.M. Theoretical aspects of electrochemical activation. Second international symposium. Electrochemical activation. Abstracts and short reports. Part 1. 1999. P.39 - 49 )

The catholyte has a high biological activity, since it increases the intensity of electrochemical processes that constantly occur in a living cell. Catholyte is a powerful biostimulant that accelerates the production of endogenous hormones, stimulates the secretory function of the intestine, normalizes metabolic processes, and the redox potential of cells. In addition, the catholyte containing active K ⁺ ions, stimulating the receptors of the endocrine glands, is a regulator of insulin secretion. The catholyte containing Mn + ions enhances the hypoglycemic effect of insulin, lowers blood sugar, counteracts fatty degeneration of the liver, reduces the level of fats in the body, provides physiological regulation of carbohydrate metabolism, and can be successfully used in the pathogenetic treatment of diabetes.

When a direct electric current is passed through an aqueous solution, electroactivation of molecules, atoms, ions and redistribution of ions in the electric field occurs in it. As a result, an aqueous solution located in the cathode zone of the electrolyzer (catholyte - EVR-K) acquires reducing properties.

It is known to use electroactivated aqueous solutions (EVR) of metal salts along with generally accepted methods for the prevention and treatment of diabetes. The properties and degree of EVR activity are determined by the parameters of pH and redox potential (ORP) (Bluvshtein G.A., Kulakov A.A. Experience in the use of electrochemically active solutions (ECR) in the treatment of patients with purulent-necrotic complications of diabetes mellitus. // Abstracts reports and short reports of the First International Symposium “Electrochemical Activation in Medicine, Agriculture, and Industry.” M., 1997. P.77.)

Closest to the claimed object is a method for the treatment of type 2 diabetes mellitus using electroactivated aqueous solutions (EVR) of metal salts (Ashbah D. Living and dead water - the latest medicine of our time. M. 2008). Electroactivated water proposed by the author has healing properties, primarily negatively charged water (catholyte). As an antiseptic, deodorant, positively charged water (anolyte) is used, mainly for topical use in the treatment of eczema, neurodermatitis, allergic dermatitis, acne vulgaris, furunculosis, and throat sanitation by rinsing with anolyte solution.

The disadvantage of this method is the limitation of the use of electroactivated aqueous solutions (EVR) of metal salts, namely, anolyte solution for the treatment of type 2 diabetes mellitus, complicated by secondary diseases caused by bacterial parasitic infections that disrupt, primarily, the gastrointestinal tract.

The aim of the invention is to expand the scope of use of electroactivated aqueous solutions (EVR) of metal salts for the treatment of type 2 diabetes mellitus, complicated by parasitic diseases, by heating the solutions (+ 36 ° C - + 37 ° C).

It is known to use electroactivated aqueous solutions (EVR) of metal salts in the complex treatment of parasitic and infectious diseases (US Pat. RF No. 2254140, publ. 06/20/2005). This method, as defined by the authors of the patent, allows to achieve immunostimulating and bactericidal actions that can have solutions with positive (anolyte) and negative (catholyte) redox potential. However, this method does not allow to obtain the expected effect of exposure to positively charged EVR with anolyte in the treatment of diabetes mellitus, because A “pure” anolyte, prescribed in the first days of treatment, can cause impaired gallbladder motility - dyskinesia, an inflammatory process in the form of stoneless cholecystitis, which tends to transform into gallstone disease. That is why anolyte, prescribed in the first days of treatment, it is desirable to accompany drugs and vitamins that can have a gentle effect on the gastrointestinal tract.

The goal is achieved by the fact that for the treatment of diabetes complicated by concomitant diseases, use electroactivated aqueous solutions of inorganic salts, and while taking medications that help regulate carbohydrate metabolism and reduce the excretion of sugar in the urine, patients are prescribed an anolyte solution of EVR in the first 5 days of treatment -A with ORP characteristics from +700 to +800 mV and with a pH from 6.0 to 4.5.

Starting from the 6th day for 5 days, patients are prescribed per os a solution of EVR-K catholyte with AFP - 400 to - 600 MB and a pH from 9.5 to 10.5.

Starting from the 11th day for 10 days, patients are prescribed per os a solution of EVR-K catholyte and an EVR-A anolyte solution, alternating EVR-K and EVR-A solutions during the day.

The EVR-A anolyte solution is prescribed in a mixture with aqueous solutions of antiparasitic herbal preparations in a volume ratio of 1: 3 in the form of rectal microclysters during the first 5 days, and probiotics based on living microorganisms are added to rectal microclysters with catholyte starting from 6 days within 10 days - Bifidobacterium adolescentis and / or Lactobacillus acidophilus, and / or Escherichia coli in liquid form. It is important at the same time that the EVR-A and EVR-K solutions are prescribed in a heated form (+ 36 ° C - + 37 ° C). In cases of detection of opisthorchisis in the patient’s intestines, parasites are poisoned with biltricide at the rate of 75 mg / kg on the 15th day, and on the 16th day therapeutic duodenal sounding is performed with washing of the bile ducts first with anolyte solution, after 30 minutes with catholyte solution, and then after 30 minutes with a solution of a therapeutic antiparasitic drug, for example, ecorsol.

Starting from day 6, until the end of the treatment cycle (day 21), patients are prescribed “NARINE” - a fermented milk product made from live lactic acid bacteria Lactobacillus acidophilus EP 317/402 in a dosage of 1 tbsp. 3 times a day 30-40 minutes before a meal.

Of the B vitamins, it is desirable to use thiamine, riboflavin, cobalamin, since thiamine has a normalizing effect on gastric motility and acidity of the gastric juice, affects fat metabolism; Riboflavin, which is found in rose hips, black currants, cabbage, tomatoes, carrots, is necessary for the synthesis of protein and fat, contributes to the normal functioning of the stomach and liver, and cobalamin is an indispensable product for the life of the intestinal flora.

As a rule, for the prevention and treatment of parasitoses and chronic infections that accompany the underlying disease, numerous chemical and pharmaceutical agents are used, primarily antibiotics and sulfa drugs. In the case of such a serious ailment as diabetes, the more so as the disease progresses and complications develop, the number of medications taken increases, the likelihood of adverse reactions is increased. Modern medicine considers the disease in isolation from the entire complex of negative factors that aggravate the course of the disease, however, only a holistic approach to a person can bring the desired results in the fight against one or another serious illness.

The authors profess this ideology and consider chronic diseases as systemic ailments resulting from the defeat of many organs. In particular, in diabetes mellitus, the liver, hepatic ducts, duodenum, small and large intestines, and pancreas are inevitably involved in the pathological process. And parasitic diseases, such as opisthorchiasis, giardiasis, helicobacteriosis, so aggravate the general condition of patients that no less effective drugs should be added to the complex of special drugs aimed at regulating blood sugar and restoring carbohydrate metabolism at the stage of impaired glucose tolerance to suppress parasitic infections, supporting as much as possible the work of vital organs, such as the pancreas, responsible for insulin secretion to overcome insulin resistance.

It is proved that anolyte has a selective ability: destroying pathogenic microorganisms, it leaves unharmed the beneficial microflora. This property of the anolyte gives it a huge advantage over antibiotics: destroying the pathogenic flora, they simultaneously destroy the beneficial flora necessary for the normal existence of the body, which leads to a violation of the immune and enzymatic functions. The inventors have proved that, having antiseptic, anti-allergic, anti-inflammatory, etc. properties, anolyte (positively charged EVR) can, in combination with catholyte (negatively charged EVR), have a beneficial effect on the inflammation focus, to a large extent when taken orally as a therapeutic drink. Moreover, unlike catholyte, anolyte retains its properties for a rather long time.

In the practice of prevention and treatment of diabetes mellitus, one should not refuse from generally accepted methods, but use them in combination with electroactivated solutions. The properties and degree of activity of the EVR are determined by the parameters of pH and redox potential (ORP).

Treatment of diabetes should be carried out comprehensively, based on the main principle - the maximum compensation of impaired metabolic processes, the most affordable indicators of which are normalization of blood sugar and elimination of urinary sugar.

The practice of treating diabetes mellitus with the proposed method was preceded by the experimental part, as a result of which the main stages of clinical research and the implementation of the method according to the invention were determined.

The aim of the experiment was to study the mechanism of the effect of electroactivated aqueous solutions of metal salts on a living organism during the development of diabetes mellitus.

To this end, an experiment was conducted on adult Wistar rats weighing 200-250 g. To simulate diabetes mellitus, animals were injected subcutaneously with 10% alloxan solution at the rate of 0.1 ml / 100 g body weight. On days 1, 3 and 6 of the experiment, blood samples were taken from the animals by notching the tail to determine the glucose level. On day 6, animals were landed in metabolic cages to collect urine. At the end of the experiment, blood samples were taken from the inferior vena cava for all animals for subsequent determination of ion osmotic parameters, as well as pancreatic and kidney tissue samples. The concentration of glucose in blood plasma and urine was determined by the picrin method.

All animals were divided into 2 groups - control and experimental, each of which, in turn, was divided into three subgroups: animals receiving water, a solution of catholyte (EVR-K) or anolyte (EVR-A) (table 1).

Table 1 The concentration of glucose in the blood plasma of rats (M ± m), mmol / l Experiment time, day Control (K) Diabetes mellitus (diabetes) input EEVR-K EVR-K EVR-A + EVR-K water EVR-A EVR-K EVR-A + EVR-K one 6.7 ± 0.3 5 ± 0.35 6.6 ± 0.18 6.8 ± 0.45 14.7 ± 3.4 * 9.06 ± 1.15 ^{▲ ∆ *} 23.06 ± 4.03 * 7.06 ± 1.15 ^{▲ ∆} 3 18.1 ± 4.0 * 11.3 ± 1.4 ^{▲ ∆ *} 23.18 ± 5.01 * 9.3 ± 1.4 ^{▲ ∆ *} 6 16.1 ± 5.3 * 10.2 ± 3.2 * ▲ 12.05 ± 2.8 * ∆ 8.2 ± 1.2∆ ▲ * 10 24 ± 6.2 * 15.4 ± 4.2 * ▲ 16.2 ± 5.3 ▲ * 12.4 ± 2.1 * ▲ Note (hereinafter): * - significant differences from similar control indicators (p≤0,05); ∆ - significant differences between the experimental groups (catholyte, anolyte) (p≤0,05); ▲ - significant differences compared with the water group (p≤0,05)

From the data of table 1 it is seen that in healthy animals, catholyte and anolyte did not cause changes in blood glucose levels throughout the observation period. In the animals of the experimental group, on the first day after the injection of alloxan, the glucose level was significantly higher than the similar control indices, which indicated the development of diabetes mellitus. Only in the EVR-A group, this indicator was significantly lower than the indicators of the other groups, and in the EVR-A + EVR-K group, it did not significantly differ from the control. A similar situation persisted on the 3rd day of the experiment. On the 6th day of observation, the glucose level in experimental animals in the EVR-A + EVR-K group significantly differed between the groups, but was higher than intact animals. On the 10th day of observation, the glucose level in experimental animals in the “EVR” group did not significantly differ between the groups, but was lower than the “water” group. Thus, we can conclude that in healthy animals, electrochemically active aqueous solutions did not cause changes in blood glucose, while against the background of diabetes mellitus, in all groups of "EVR" there was a hypoglycemic effect, the most pronounced in the group "EVR-A + EVR-K ".

When analyzing the ion-osmotic parameters of rat blood plasma and urine, it was found that in intact animals various aqueous solutions did not cause significant changes in ion concentration and indicators of renal function, except for a decrease in urea in blood plasma after administration of EVR-A. Against the background of diabetes mellitus, the concentration of sodium and potassium did not change, and the urea content tended to increase, and the creatinine level was significantly higher than that of healthy animals. The changes obtained may indicate a violation of the functional state of the kidneys and the development of diabetic nephropathy in conditions of diabetes. At the same time, no significant differences were found among animals that received different electrochemically activated aqueous solutions, with the exception of a slight increase in creatinine concentration and hypokalemia after taking EVR-K (Table 2).

table 2 Rat ion plasma osmotic parameters (M ± m) Concentration, mmol / L The control SD water EVR-A EVR-K water EVR-A EVR-K sodium 139.4 ± 13.5 138.3 ± 12.1 129.7 ± 13.0 148.0 ± 23.7 124.0 ± 8.6 131.0 ± 34.1 potassium 4.1 ± 0.2 3.9 ± 0.2 4.0 ± 0.3 3.8 ± 0.2 3.6 ± 0.3 3.0 ± 0.3 * ^▲ creatinine 1.14 ± 0.04 1.1 ± 0.05 1.09 ± 0.02 1.4 ± 0.03 * 1.4 ± 0.04 * 1.5 ± 0.01 * ^{▲ ∆} urea 54.0 ± 4.9 33.8 ± 5.6 ^{▲ ∆} 49.8 ± 4.8 60.0 ± 7.7 64.8 ± 7.2 * 63.6 ± 8.7 Note: see table 1

A study of the hydro- and ion-uretic functions of the kidneys revealed an increase in the level of diuresis against diabetes mellitus in groups of animals that took both anolyte and catholyte (Table 3). Glomerular filtration rate and tubular reabsorption level did not differ between diabetes and control groups. At the same time, the excreted potassium fraction increased, which most likely reflected an increase in cation secretion. These facts indicate in favor of increased excretion of osmotically active substances (for example, glucose) under the influence of ESR in conditions of diabetes, as a result of which the rate of urination and purification of potassium increased. Since the functional changes in the organ are due to changes in its morphological structure, the next stage of our study was an analysis of the morphological structure of the pancreas and kidneys of animals.

Table 3 Hydro and ion uretic kidney function in rats (M ± m) Indicator the control SD water EVR-A EVR-K water EVR-A EVR-K V, ml / 100 g * hour 0.05 ± 0.02 0.03 ± 0.01 0.04 ± 0.01 0.06 ± 0.01 0.11 ± 0.02 * ^▲ 0.11 ± 0.03 * GFR, ml / 100 g * hour 13.7 ± 3.4 20.6 ± 2.12 12.2 ± 2.5 ^∆ 10.4 ± 2.0 15.2 ± 3.7 12.2 ± 4.9 RH ₂ o,% 99.6 ± 0.1 99.7 ± 0.03 99.6 ± 0.09 99.6 ± 0.04 99.7 ± 0.1 99.0 ± 0.6 U _Na * V, μmol / 100 g * hour 4.0 ± 1.5 13.7 ± 0.6 ^{▲ ∆} 7.0 ± 2.8 4.3 ± 1.6 6.8 ± 2.4 * 16.5 ± 5.5 ^∆ U _K * V, μmol / 100 g * hour 15.6 ± 6.4 12.0 ± 1.5 8.9 ± 3.1 17.1 ± 8.6 16.6 ± 1.07 17.1 ± 6.7 EF _Na ,% 0.2 ± 0.1 0.4 ± 0.05 0.4 ± 0.2 0.3 ± 0.09 0.3 ± 0.10 2.11 ± 0.97 EF _K ,% 22.8 ± 10.2 15.03 ± 3.3 13.06 ± 2.8 54.2 ± 6.2 40.6 ± 2.2 * 58.5 ± 11.8 *

It was established that in all experimental groups, compared with the control, necrotic changes caused by intoxication with alloxan were observed. When analyzing the structural organization of the pancreas of rats after administration of EVR-A and EVR-K, only the annular transformation of the acinus, hypertrophy of the islets of Langerhans, and subnecrosis were found. A different picture was observed in the structural organization of the pancreas of rats who consumed water in the alloxan model of diabetes mellitus. Along with the annular transformation of the acinus, hypertrophy of the islets of Langerhans, subnecrosis, which were observed in the group of CD + EVR-A, hypertrophy and hyperplasia of the islets of Langerhans, interlobular edema, focal necrosis of the tissue, pseudocystosis, as well as connective tissue replacement of atrophy, and atrophic connective tissue atrophy are noted. proteolysis in combination with lipolysis Thus, administration of EVR-A and EVR-K against the background of diabetes contributed to minimal structural changes in the pancreas compared to water.

At the same time, anolyte against the background of the studied pathology contributes to a delay in the development of this disease, which is manifested in lower levels of glucose in the blood, while catholyte does not have such a protective effect. Histological analysis showed a positive effect of anolyte on the pancreas structure of rats with an alloxan model of diabetes mellitus. The data obtained require the study of biochemical processes that determine the similar hypoglycemic and protective effects of anolyte.

The next stage of the experiment was to evaluate the effectiveness of taking an EVR solution in cold and warm form. To this end, an experiment was conducted on adult Wistar rats weighing 200-250 g with experimental diabetes mellitus. Five rats were taken, which were fed with EVR-A + EVR-K solutions alternately at a solution temperature of 36-37 ° C. The blood sugar indices in the “EVR-A + EVR-K” group were taken for control, which were watered with solutions with a temperature of 21-23 ° C (see table 4)

Table 4 Rat blood sugar in the dynamics of diabetes The time of the experiment, day Control (K) (intact) (mp 21-23 ° C) SD + EVR-A + EVR-K (t-ra 36-37 ° С) Significant differences from K one 7.06 ± 1.15 6.8 ± 2.4 p≥0.05 3 9.3 ± 1.4 7.8 ± 1.3 p≤0,05 6 8.2 ± 1.2 6.9 ± 1.25 p≤0,05 10 12.4 ± 2.1 10.2 ± 2.1 p≤0,05

Thus, studies have shown that the temperature of solutions affects the results of blood sugar. When rats with diabetes mellitus were fed with EVR-A + EVR-K solutions with a temperature of + 36 ° C - + 37 ° C, blood sugar parameters significantly differed from the control group.

Clinical observations.

We have treated patients with diabetes mellitus type 1 (10 people) and type 2 (12 people) using EVR solutions. In patients with type 1 diabetes mellitus against the background of insulin, warm EVR solutions (+36 - + 37 ° С) were prescribed inside as follows: within 5 days, the intake of EVR-A solution (ORP = +700 - +800 mV); in the next 5 days - EVR-K (ORP = -450 - -600 mV);

Over the next 10 days, the intake of EVR-A and EVR-K of the indicated parameters was alternated during the day according to the following scheme: in the morning - EVR-A, in the afternoon - EVR-K; for dinner - EVR-A; before going to bed - EVR-K.

Patients with type 2 diabetes mellitus were canceled their antidiabetic drugs and treatment was prescribed according to the same scheme using solutions of EVR-A and EVR-K. In the presence of opisthorchiasis, deworming was performed using the drug biltricid.

In all cases, received a positive effect. In the group with type 1 diabetes mellitus, a 2-fold reduction in insulin injections was achieved; in the group of patients with type 2 diabetes mellitus - complete cancellation of sugar-lowering drugs was achieved in 8 patients (67%), in the remaining 4 patients - the dose of drugs was reduced by 2 times.

Example 1. Patient P., 24 years old, the diagnosis is “type 1 diabetes mellitus, insulin-dependent, sick for 7 years. Treatment:

for breakfast - 6.0 units. insulin

for lunch - 3 units. insulin

for dinner - 3 units. insulin

Blood sugar level in the morning, on an empty stomach, before taking insulin - 12 mmol / L.

Against the background of insulin intake, the intake of EVR solutions was prescribed according to the following scheme: within 5 days, the intake of EVR-A solution (ORP = +700 - +800 mV); in the next 5 days - EVR-K (ORP = -450 - -600 mV);

Over the next 10 days, the intake of EVR-A and EVR-K of the indicated parameters was alternated during the day according to the following scheme: in the morning - EVR-A, in the afternoon - EVR-K; for dinner - EVR-A; before going to bed - EVR-K. Doses of insulin were halved: 3 units; 1.5 units; 1.5 units

Blood sugar levels with insulin in the morning on an empty stomach:

1st day - 14 mmol / l;

3rd day - 16 mmol / l;

6th day - 12 mmol / l

10th day - 10 mmol / l

13th day - 9 mmol / l

16th day - 6 mmol / l

20th day - 5.4 mmol / l

Blood sugar levels were kept low for 1 year of observation.

Example 2. Patient Ш, 42 years old, doctor of health: “Type 2 diabetes mellitus, has been ill for 10 years. Concomitant diseases: chronic opisthorchiasis, allergic rhinitis, recurrent urticaria. Examined, performed duodenal sounding, during which eggs of opisthorchis were identified.

The patient took 2 tablets of mannin. in the morning on an empty stomach. The blood sugar level before taking the tablets is 10 mmol / L. The patient underwent deworming of opisthorchis with a biltricid at the rate of 75 mg / kg of patient weight. Then, while taking an EVR solution, mannin was canceled.

The patient was prescribed to take EVR solutions heated to +36 - + 37 ° С according to the following scheme: within 5 days, receive an EVR-A solution (ORP = +700 - +800 mV); in the next 5 days - EVR-K (ORP = -450 - -600 mV).

Over the next 10 days, the intake of EVR-A and EVR-K of the indicated parameters was alternated during the day according to the following scheme: in the morning - EVR-A, in the afternoon - EVR-K; for dinner - EVR-A; before going to bed - EVR-K.

Within 5 days, a solution of anolyte EVR-A in a mixture with aqueous solutions of antiparasitic herbal preparations in a volume ratio of 1: 3 in the form of rectal microclysters was prescribed.

Starting from day 6, rectal microclysters with catholyte were performed for 10 days, to which probiotics based on living microorganisms - Bifidobacterium adolescentis, and / or Lactobacillus acidophilus, and / or Escherichia coli in liquid form were added.

On day 16, therapeutic duodenal sounding was performed with washing of the bile ducts first with anolyte solution, after 30 minutes with a catholyte solution, and then after 30 minutes with a solution of the therapeutic antiparasitic drug ecorsol.

Starting from day 6, until the end of the treatment cycle (day 21), the patient was prescribed per os "NARINE" - a fermented milk product made from live lactic acid bacteria Lactobacillus acidophilus EP 317/402 in a dosage of 1 tbsp. 3 times a day 30-40 minutes before a meal.

On day 16, therapeutic duodenal sounding was performed with washing of the bile ducts first with anolyte solution, after 30 minutes with a catholyte solution, and then after 30 minutes with a solution of the therapeutic antiparasitic drug ecorsol. As a result of treatment: Fasting blood sugar indicators:

1st day - 12 mmol / l;

3rd day - 14 mmol / l;

6th day - 10 mmol / l

10th day - 8.5 mmol / l

13th day - 9 mmol / l

16th day - 7.2 mmol / l

20th day - 6.4 mmol / l

During the year of observation, the patient did not take any sugar-lowering drugs.

The clinical picture of the improvement is confirmed by the data of a weekly study of blood sugar and urine, while there is a gradual decrease in blood sugar and cholesterol to normal and subnormal figures at the end of treatment.

Positive dynamics of clinical symptoms is observed already on the 5-6th day of treatment: symptoms such as dry mouth, excessive urination, weakness, itching of the skin are reduced or stopped, and intestinal function is regulated. Almost all patients have an improvement in test results:

- decrease in blood glucose, glycosylated hemoglobin, total and "bad" cholesterol;

- decrease in insulin intake;

- stopping surges in sugar levels.

EVR-K favorably affects the course of secondary gastritis and colitis that developed against the background of diabetes, prevents fatty degeneration of the liver, and stimulates the protein-forming function of the liver. EVR-K corrects cardiovascular complications of diabetes mellitus, improving myocardial trophism. In diabetes mellitus resistant to conventional therapy, treatment with EVR-K solutions is continued for a month, then repeated quarterly according to a specially developed technique. After reaching normal values of blood sugar to prevent the progression of the disease, it is recommended that you drink EVR-K at least 2-3 times a week.

How can catholyte be useful for diabetes? Its ions K activate receptors of the endocrine glands, including the pancreas. The result is improved insulin secretion. This is supplemented by the action of Mn ions contained in the solution. As mentioned above, these ions enhance the sugar-lowering effect of insulin, lower the amount of lipids in the blood. In other words, Mn ions are involved in the regulation of carbohydrate and fat metabolism in the body, inhibit the accumulation of lipids in liver cells, which over time can lead to fatty degeneration of the liver. Depending on the pH of the solution and its redox potential, the properties and degree of activity of the electroactivated aqueous solution change.

In the treatment of diabetes mellitus, EVR-K with the following characteristics is used: ORP = 400-450 mV; pH 9.5-10.5. Take the resulting solution should be 150 ml 3 times a day for 20-30 minutes before meals for at least 4 weeks. EVR-K is prepared daily and stored in a hermetically sealed container in a dark, cool place.

The proposed treatment regimen is also suitable for patients with diabetes mellitus, uncomplicated concomitant diseases. By the 4-5th week of taking the solution, as a rule, a distinct decrease in blood sugar level is observed, although the first positive symptoms may appear on the 5-6th day of treatment (dry mouth, normal amount of urine being separated, skin itching disappears, intestinal function improves - the symptoms of gastritis and colitis that arise on the background of diabetes mellitus disappear). The protein synthesizing function of the liver is activated. The tests performed indicate that during treatment, the level of sugar in the blood and urine and the level of cholesterol in the blood gradually decrease.

Claims (2)

1. A method for the treatment of diabetes mellitus, comprising taking medications that regulate carbohydrate metabolism and administering electroactivated aqueous solutions of inorganic salts, characterized in that anolyte solution of an electroactivated aqueous solution of inorganic salts (EVR-A) with redox potential is administered (ORP) from +700 to +800 mV and with a pH from 6.0 to 4.5; starting from the 6th day, a solution of catholyte of an electroactivated aqueous solution of inorganic salts (EVR-K) with an ORP of -400 to -600 mV and a pH of 9.5 to 10.5; starting from the 11th day, an EVR-K catholyte solution and an EVR-A anolyte solution are alternated over a period of 10 days, moreover, the EVR-A and EVR-K solutions are administered heated to + 36 ° C - + 37 ° C . 2. The method according to p. 1, characterized in that with concomitant parasitic invasion during the first 5 days of treatment, an EVR-A anolyte solution is mixed with aqueous solutions of antiparasitic herbal preparations in a volume ratio of 1: 3 in the form of rectal microclysters; starting from the 6th day, a solution of EVR-K catholyte with ORP from -400 to -600 mV and pH from 9.5 to 10.5 in combination with rectal microclysters with catholyte with the addition of probiotics based on living microorganisms is administered for 10 days Bifidobacterium adolescentis and / or Lactobacterillus acidophilus and / or Escherichia coli in liquid form; if opisthorchisis is detected, a biltricid at a dose of 75 mg / kg is administered on the 15th day, and duodenal sounding is performed on the 16th day with rinsing of the bile ducts first with anolyte solution, after 30 minutes with catholyte solution and then after 30 minutes with therapeutic antiparasitic solution a drug, for example ecorsol; in addition, from day 6 until the end of treatment (day 21), the “Narine” fermented milk product from live lactic acid bacteria Lactobacillus acidophilus EP 317/402 is prescribed in a dosage of 1 tbsp. 3 times a day 30-40 minutes before meals.