RU2526203C1 - Method of production of anti-luteolytic blood - alb, method of treatment and prevention of postpartum obstetric and gynecological diseases in cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: method for production of anti-luteolytic blood - AlB is that luteolisate is administered subcutaneously to gelding twice with an interval of 14 days at a dose of 20 ml each, containing parts of the corpus luteum of pregnancy of cows, and after 14 days after the second administration the blood is taken from the jugular vein. The method of treatment and prevention of persistent corpus luteum, subinvolution of uterus and postpartum endometritis in cows comprises at the background of general therapy the use of anti-luteolytic blood that is administered subcutaneously in the neck region twice at a dose of 10 ml each with an interval of 6 days.

EFFECT: use of anti-luteolytic blood promotes the resorption of the corpus luteum of pregnancy and shortens the duration of postpartum involution of genitals that enables to prevent and reduce the time of treatment of ill cows with persistent corpus luteum, subinvolution of uterus and postpartum endometritis.

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Description

The invention relates to the field of veterinary obstetrics and can be used for the prevention and treatment of obstetric and gynecological diseases in cows.

In the reproductive system of cows, after normal birth, large changes occur. 2-3 hours after birth, the uterine cavity is collapsed. The uterus becomes dense, folds are felt on its surface. Caruncles fill almost its entire lumen. The horn that served as the repository is more free. On the 3-5th day, the lumen of the cervical canal is narrowed so that it passes only 2-3 fingers. Cows in the early days are bloody; on the 3-5th day they are in the form of thick, muddy, viscous mucus. Then the suckers become chocolate color; from the 8th day they acquire the character of mucus, and by the 10-14th day they stop.

After birth, the number of corpus luteum cells (Corpus luteum) of pregnancy gradually begins to decrease. Vessels, abundantly piercing the corpus luteum, converge with a decrease in its total size, forming a dense network. Gradually, these vessels also undergo degeneration, turning into connective tissue cords. Involution of the corpus luteum of pregnancy ends by the 16th day of the postpartum period and is accompanied by intensive growth of follicles [2].

Under optimal conditions of feeding, care, maintenance and proper operation, the natural postpartum involution of the genitals in cows ends on average 3 weeks after giving birth, they develop intensively in the ovaries in the ovaries of the ovum, produce estrogens (follicular hormone), and when inseminated, it can again occur fertilization and pregnancy [9].

In case of poor feeding and unsatisfactory conditions of keeping highly productive cows, especially in the absence of walks in the stall period, the involution process lasts up to 40 or more days, such cows often lack sex cycles (anaphrodisia), and rectal examination reveals yellow bodies in the ovaries [ 3, 8].

The corpus luteum remaining after childbirth maintains a high level of progesterone in the body. This hormone inhibits the growth of follicles and their ovulation, delays postpartum involution [2].

D. Hammond (1955) indicates that cows under the influence of the follicular hormone increase the uterus's resistance to infection and the risk of infection when microbes enter the uterus is much less than cows that are affected by the corpus luteum hormone. He explains this by saying that the mucus released under the influence of estrogen creates a barrier to microbes, while the mother’s milk released under the influence of progesterone provides a good breeding ground for them. In addition, estrogen hormones increase the protective functions of the uterus by enhancing the leukocyte reaction [5].

Thus, as a result of impaired resorption of the corpus luteum of the pregnancy in the postpartum period, the cows disrupt the antimicrobial and contractile function of the uterus, which contributes to the development of subinvolution of the uterus and postpartum endometritis, prolonged violations of the reproductive cycles and infertility are observed [1, 2, 3, 4, 5, 6 , 7, 8].

With inflammatory processes in the uterus, the production of prostaglandin F2a is disturbed, as a result of which no regression of the corpus luteum occurs, which is delayed for a long time. Therefore, when examining cows with uterine subinvolution and postpartum endometritis, yellow bodies are often found in the ovaries.

Based on this, the main task of the modern comprehensive prevention of uterine subinvolution, postpartum endometritis and persistent corpus luteum in cows is to use drugs for timely resorption of the corpus luteum, restore tone and contractile function, stimulate the regeneration of epithelial tissues in the uterus, and increase the general resistance of the body [3, 5 ].

The purpose of the invention is the preparation and use of anti-luteal blood (ALK), which has a therapeutic and prophylactic effect aimed at increasing the body's defenses, accelerating the resorption of the corpus luteum of pregnancy and involution of the uterus in cows.

The claimed invention meets the criterion of "novelty", since the use of antiluteal blood (AlK) for the prevention and treatment of cows with a persistent corpus luteum, subinvolution of the uterus, postpartum endometritis is not known.

The claimed invention meets the criterion of "significant differences", as there is an additional effect - postpartum obstetric and gynecological diseases are prevented and the treatment time for sick cows is reduced.

To obtain anti-luteal blood (ALK), a young healthy gelding is used, which is subcutaneously injected with a luteolysate of cows containing parts of the corpus luteum of pregnancy at a dose of 20 ml, twice with an interval of 14 days. The introduction of a foreign agent promotes the formation of biologically active substances in the body of the gelding, which have a directed action that accelerates the resorption of the corpus luteum of pregnancy and the involution of the uterus of cows. 14 days after the second injection of luteolysate, blood is taken from the jugular vein from the jelly and an anticoagulant is added.

There is a method of using prostaglandin-containing drugs (magestrofan, estrofan, dinoprost, enzaprost, etc.) with persistent corpus luteum and corpus luteum cysts in cows, but these drugs do not have a generally stimulating effect. Studies have shown that the therapeutic and prophylactic effect of magestrofan in the postpartum period is inferior to the effectiveness of using antiluteal blood (AlK).

A known method of using the preparation of fatty acids in persistent corpus luteum in cows, but it contains only gonadotropic hormones and does not have a targeted action aimed at resorption of the corpus luteum.

Used autohemotherapy of cows with a persistent corpus luteum - double intramuscular injections of 50-100 ml with an interval of 5 days, has only a general stimulating effect and does not promote resorption of the corpus luteum [3, 5].

However, antiluteal blood (ALK), in comparison with conventional autologous blood, in addition to general stimulating blood, also has a specific effect aimed at enhancing resorption of the corpus luteum of pregnancy, which accelerates the postpartum involution of the genital organs. The effectiveness of AlK is enhanced by the use of uterotonic drugs.

Antiluteal blood (AlK) is administered to stimulate the postpartum involution of the genital organs of newborn cows and to prevent postpartum obstetric and gynecological diseases (subinvolution of the uterus, postpartum endometritis, persistent corpus luteum) subcutaneously in the neck twice in 10 ml, immediately after fetal removal and after 6 days.

For therapeutic purposes, with a persistent corpus luteum, uterine subinvolution, postpartum endometritis of cows, anti-luteal blood (AlK) is injected subcutaneously in the neck twice in 10 ml, with an interval of 6 days, against the background of general therapy.

Tests of antiluteal blood (AlK), against the background of the complex treatment of sick cows with a persistent corpus luteum, uterine subinvolution, postpartum endometritis (5 sick animals, respectively) showed high therapeutic efficacy.

When anti-luteal blood (ALK) was used prophylactically for 20 newborn cows, a persistent corpus luteum, uterine subinvolution and postpartum endometritis were not observed. In experimental cows, a shortening of the time for hocking and uterine involution was noted.

The use of anti-luteal blood (AlK) accelerates the resorption of the corpus luteum and reduces the duration of postpartum involution of the genital organs, which allows to prevent and shorten the treatment time for sick cows with persistent corpus luteum, subinvolution of the uterus and postpartum endometritis.

Information sources

1. Veterinary obstetrics and gynecology. Ed. prof. G.A. Kononova. - L., "Kolos" (Leningrad branch), 1977. - S.350-361.

2. Veterinary obstetrics, gynecology and biotechnology of reproduction / A.P. Studentsov, V.S. Shipilov, V.Ya. Nikitin, etc .; Ed. V.Ya. Nikitin and M.G. Mirolyubov. - 7th ed., Revised. and add. - M: Kolos, 2000 .-- S.315-317.

3. Goncharov V.P., Karpov V.A. Prevention and treatment of gynecological diseases of cows. - 2nd ed., Revised. and add. - M .: Rosagropromizdat, 1991 .-- 190 p.

4. Ermachenkov N.N. Obstetrics and gynecology of farm animals. Ed. 2nd, rev. and additional - L., "Kolos" (Leningrad. Department), 1976. - S.181-182.

5. Zayanchkovsky I.F. Prevention and treatment of obstetric and gynecological diseases in cows. - Ufa, Bashkir book publishing house, 1982. - 232 p.

6. Logvinov D.D. Pregnancy and childbirth in cows. - K., "Harvest", 1975, S.209-229.

7. Workshop on obstetrics, gynecology and biotechnology of animal reproduction. / V.Ya. Nikitin, M.G. Mirolyubov, V.P. Goncharov and others - M .: KolosS, 2004. - P.158-168.

8. Titova V. et al. Impact on uterine involution in cows by the quantum method. // Titova V., Nasibov F., Baytlesov E., Katyukov V., Vlasova G. / Dairy and beef cattle breeding, No. 1, 2006. - S.24-25.

9. Shipilov V. S. Physiological basis for the prevention of infertility in cows, M., 1977.-288 S.

Claims (2)

1. A method of obtaining antiluteal blood - AlK, characterized in that the gelding is used, which is administered twice a day with an interval of 14 days, a luteolysate in a dose of 20 ml containing parts of the corpus luteum of the cow is taken, and 14 days after the second injection, blood is taken from the jugular vein . 2. A method for the treatment and prevention of persistent corpus luteum, subinvolution of the uterus and postpartum endometritis in cows, including the use of anti-luteal blood - AlK as a part of general therapy, characterized in that the drug uses anti-luteal blood - AlK, which is injected subcutaneously in the neck twice 10 ml with an interval of 6 days.