RU2523415C1 - Method of obtaining mineral silicic water - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: invention relates to a method of obtaining mineral silicic water (MSW), intended for application for medical purposes. The method of obtaining includes hydrolysis of tetraethoxysilane in the TEOS mixture: ethanol: water, acidified by HCl. Nanosol is obtained at a temperature of 55-65°C for 1.5 hours with evaporation of ethanol to the volume reduction by 1/3, then, dilution of the obtained nanosol with a physiological solution NaCl is carried out in 2 steps with equal portions of the physiological solution, preliminarily heated to 40-50 in a ratio of volumes of the initial nanosol: physiological solution 1:7 with 15-minute interval. After each dilution a temperature of the solution is kept in the range of 55-65°C.

EFFECT: increase of the compound application efficiency.

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Description

Technical field

The invention relates to nanotechnology, in particular to the production of mineral silicon water (MKV) containing silicon in the form of nanoparticles of amorphous silica (SiO ₂ ) and sodium chloride, intended for medical use, in particular, to increase the energy status of the body, as an immunomodulator , to improve metabolic processes in the body, inactivator of toxic proteins of various origins, treatment of benign tumors, as an oncoprotector, and treatment of diabetes 2.

State of the art

It is known that no living organism can exist without silicon. The healing properties of silicon have long been known in folk medicine, and now it is firmly included in the medical practice of official medicine. A new drug of effective therapy (Chuyko O.O., Pogorely V.K., Pentyuk O.O. // Medical chemistry and clinical application of silicon dioxide // / 1 /, is, for example, a tablet powder of pyrogenic silica nanoparticles. It is called "Silix" and is successfully used in the treatment of gastrointestinal diseases as a sorbent and antidote, as well as as a corrector for many pathological conditions. Amorphous silica is a non-toxic chemically inert material, its therapeutic effect is solely due to its Twomey surface. Nanoparticles amorphous silica in solution can, as in the case siliksa, bind microorganisms (agglyutinizatsii effect) and inactivate toxic proteins.

However, the preparation of silica nanoparticles in powder form usually by a pyrogenic method leads to aggregation of individual particles having a size of about 4-5 nm to aggregates with an average size of about 40 nm. Such an aggregate size is an obstacle to the penetration into cells and the intracellular action of nanoparticles, which dramatically reduces the possibility of their therapeutic use.

It is known (Patent No. 2426692, authors: Kalinin D.V., Serdobintseva V.V.) / 2 / that individual non-aggregated particles of SiO ₂ in solution are able to freely penetrate through the plasma membrane of cells without their damage and their surface to participate in intracellular biochemical processes as a kind of surface catalyst for biochemical reactions.

There is also a method of producing silica nanoparticles in an aqueous solution (application for invention No. 20112120733 dated 05/18/2012, decision to grant a patent on 05/06/2013) / 3 /, which is more applicable for solving biotechnological problems and, to a lesser extent, for medical therapeutic purposes from -for use in the process of ion-exchange resins.

The objective of the invention is to develop a method for producing a stable solution of silica nanoparticles with a size of 4-5 nm in physiological saline solution of NaCl for direct therapeutic use in the form of mineral silicon water (MKW), the therapeutic effect of which is mainly based on the restoration of the energy functions of mitochondria and, as consequence, activation of the immune system.

Disclosure of invention

The essence of the invention lies in the fact that in the known method for producing silica nanosol by hydrolysis of tetraethoxysilane (TEOS) in a mixture of TEOS: ethanol: water (mol.) Acidified with HCl to a pH of 1.5-2 = 1: 5: 6, followed by exposure of the mixture to at a temperature of 40-50 ° C for two hours, ion-exchange resins are not used, but nanosol is diluted with physiological NaCl solution.

- According to the claimed invention, for a more complete reaction of the hydrolysis of TEOS, the maturation of nanosol is carried out at a temperature of 55-65 ° C for 1.5 hours.

- According to the claimed invention, in the process of maturation of the nanosol to remove ethanol, the mixture is evaporated with a volume reduction of 1/3.

- According to the claimed invention, in order to obtain MKV, a nanosol diluted to 55-65 ° C with physiological NaCl solution heated to 40-50 ° C is diluted in two stages with equal volumes.

- According to the claimed invention, the ratio of the volumes of the initial nanosol: physiological solution of NaCl is 1:14.

- According to the claimed invention, after each dilution of the nanosol with physiological saline, it is held for 15 minutes at 55-65 ° C.

- The technical result is as follows:

- Get a clear, colorless mineral silicon water (MKV) with a weight concentration of SiO ₂ in the form of nanoparticles of 0.9-1% and NaCl of 0.8-0.9% and a pH of 6-6.5.

- Get MKV, in which silica is resistant to coagulation due to the buffering action of NaCl.

- Receive MKV, the shelf life of which is 2.5-3 months.

- They receive MKV, which has high biological activity and healing properties, mainly due to the catalytic effect of nanoparticles in the mitochondria of cells, increased bioenergetic efficiency of mitochondria, as well as inactivation of toxic proteins.

Justification of the introduced features

For the first time, an MQW containing silicon in the form of silica nanoparticles of SiO _{2 of} size 4-5 nm is obtained using acidic silica nanosols obtained by hydrolysis of TEOS in a solution of ethanol and an aqueous HCl solution followed by maturation of the nanoparticles at a temperature of 55-65 ° C and evaporation of ethanol with a reduction the volume of the mixture by 1/3. The treatment of diluted nanosol with an anion exchange resin to eliminate acidity is excluded, and an acceptable pH level of 6-6.5 is achieved by diluting the nanosol with physiological NaCl solution heated to 40-50 ° C in two stages with exposure after each dilution for 15 minutes at a temperature 55-65 ° C to obtain MKV, resistant to coagulation of silica.

Despite the fact that the weight concentration of silica in the obtained mineral water is not high 0.9-1%, its biological activity (determined by A. A. Milov according to the method “Patent Control Method for Biologically Active Preparations” No. 2398227) / 4 / is extremely high, and with the use of MKV for medical purposes requires additional dilution within 5-15 drops of MVK per 50 ml of water, depending on the observed pathology, condition and age of the person. The number of nanoparticles in the therapeutic portion of MKV remains high on the order of 10 ¹² particles. Penetrating freely through the cell membrane, SiO ₂ nanoparticles then enter the mitochondria of the cells, where, corresponding to the size and function of mitochondrial ribosomes, they participate in the oxidative decomposition of organic acids as an additional surface, acting as a catalyst for bioenergetic processes.

An example of the method

Silica nanosol is prepared from a mixture of reagents: TEOS: water, acidified with HCl to a pH of 1.5-2: ethanol taken in molar ratios of 1: 5: 6, respectively. To obtain silica nanoparticles with a size of 4-5 nm, a carefully mixed mixture of reagents is kept in an open vessel at a temperature of 55-65 ° C for 1.5 hours. The volume of the mixture is reduced due to the evaporation of ethanol by 1/3. Then the nanosol is diluted with physiological saline (0.9% NaCl solution in distilled water), heated to 40-50 ° C) in a ratio of 1: 7, stirred and kept at a temperature of 55-65 ° C for 15 minutes. After this, the second dilution is also carried out with physiological saline, the temperature of which is 40-50 ° C, to the final ratio of the volumes of nanosol: MKW as 1:15 and again kept at 55-65 ° C for 15 minutes. Then, the obtained MKV is cooled to room temperature and poured into transparent, colorless glass containers for storage and use. The MKV obtained in this way is stable against clumping of individual nanoparticles and coagulation of silica for 2.5-3 months.

Claims (1)

A method of obtaining mineral silicon water using silica nanosol obtained by hydrolysis of tetraethoxysilane in a mixture of TEOS: ethanol: water, acidified with HCl as 1: 5: 6, characterized in that the nanosol is obtained at a temperature of 55-65 ° C for 1.5 hours evaporating ethanol to reduce the volume by 1/3, then the resulting nanosol is diluted with physiological NaCl in 2 stages in equal portions of physiological saline pre-heated to 40-50 °, in the ratio of the volumes of the initial nanosol: physiological solution Thief 1: 7 with an interval of 15 minutes, and after each dilution solution temperature was kept within 55-65 ° C.