RU2521974C1 - Method for radioactive substance washout from biological tissue with natural halogen-containing mineral water - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: halogen-containing hydrocarbonate chloride sodium, alkaline, boron high-magnesium, iodine and fluorine natural mineral water 'Lazarevskaya Tselebnaya' No. 84-E of the Volokonskoye deposite in Sochi is taken according to the following procedure: 30-35 minutes before meals in small sips, six times a day daily in a dose of 200-250 ml at t°=(23-24)°C, for 45 days every 2-3 days with taking the above natural halogen-containing mineral water in the same volumes for the following 45 days. The therapeutic course makes 3 years.

EFFECT: method enables improving the health status in the individuals subject to the hazardous effect of radionuclides taken with water or food.

2 cl, 6 tbl, 3 ex

Description

Technical field

The invention relates to medicine, in particular to a system of treatment and prophylactic measures aimed at accelerating the elimination of radioactive substances from biological tissues of animals and the human body.

State of the art

The problem of the removal of radioactive substances from environmental objects and living organisms is quite actively discussed in the special scientific literature from the late 40s of the last century. In particular, in 1980, the Atomizdat Publishing House (USSR) published Methodological Recommendations for the Sanitary Control of the Content of Radioactive Substances in the Environment A.N. Morey, A.S. Zykova, 1980 [Appendix 1, item 11], where the role of radioprotectors protecting the body from external radioactive infection was systematically described. At the same time, in Methodological guidelines No. 5779-91 [Appendix 1, paragraph 1], the Ministry of Health of the former USSR indicated the advisability of using effective radioprotectors for the prevention and treatment of the effects of radioactive damage. The aforementioned and subsequent documents on similar topics [Appendix 1, paragraph 2] indicate that “existing protectors are far from perfect. The reference point in the search for new chemical protective agents is the so-called ideal radioprotector, which must meet the following requirements: 1) high efficiency in the absence of toxicity; 2) the convenience of the dosage form (oral or intramuscular injection); 3) low cost in manufacturing and storage stability; the duration of the protective effect (which should start from the first minutes after administration and last for several hours); 4) the possibility of using the drug when exposed to different types of ionizing radiation. " When developing methods for monitoring the well-being of the human environment, the Federal State Scientific Institution “Federal Medical Biophysical Center named after A.I. Burnazyan ”[Appendix 1, p. 3], scientific ideas were formed that“ natural substances can also be considered as natural radioprotectors, since they, by activating the protective resources of the body and affecting the neurohumoral and immune-hematopoietic (hematopoietic) hematopoietic systems, increase the general nonspecific resistance of the body, i.e. stimulate the endogenous background of radioresistance (a complex complex of endogenous biologically active compounds: amines, thiols and other antioxidants that suppress the accumulation of excess radiation products of radiation peroxidation harmful to living cells). "

As noted by a number of domestic and foreign researchers [Appendix 1, paragraphs 4, 5, 6, 7], special methods of protection against radiation damage include measures to accelerate the elimination of radionuclides from the body that enter it and are in organs and tissues. The accelerated elimination (decorporation) of radionuclides from the body can to a greater or lesser extent prevent or interrupt the negative effects of the physiological systems of macroorganisms upon further irradiation, therefore such measures can only conditionally be considered as preventive. In addition to mechanical methods of removing radionuclides from biological tissues (frequent baths that stimulate perspiration, washing the stomach and intestines), other excretory agents are also used, such as a special diet [Appendix 1, paragraph 8] in combination with laxatives and diuretics; sorbents (and complexing agents) that bind radionuclides into structures that are rapidly excreted from the body. Of the sorbents that are currently considered promising [Appendix 1, Clause 9], ferrocin and polysimmin absorbing cesium and strontium, respectively, are also used preparations: alginates (containing polysaccharides released from algae), highly active cellulose, preparations of activated carbon, barium sulfate, etc. According to representatives of leading domestic schools of medical radiologists [Appendix 1, paragraph 10] “the most effective drug radioprotectors are representatives of two classes of chemical compounds: aptoalkilaminov and indolylalkylamines. An integral indicator of the radioprotective efficacy of radioprotectors is the value of the so-called absorbed dose reduction factor - FOOD, or dose change factor - PID, which show how many times the value of equivalent radiation doses increases when using the above pharmaceuticals. Semi-lethal doses of radiation (referred to as LD), that is, doses that cause the death of 50% of the irradiated animals, are usually compared as equally effective doses. ”

It is known to use natural substances as natural radioprotectors for removing radioactive substances from biological tissues. For example, a known method of using as a food additive natural zeolite-containing tuff according to patent 2063229 [1].

There is also a method of accelerating the elimination of radioactive substances, mainly cesium-137 and its analogues from the human body, according to patent No. 2113228 [2], which consists in taking the infusion of collecting medicinal herbs with diuretic, diaphoretic, laxative and digestive effects, and potassium-containing herbs in combination with water-thermal procedures.

The above methods for removing radioactive substances from biological tissues do not include the use of Sochi’s halogen-containing natural mineral waters for ingestion.

The mechanism of action of halogen-containing natural mineral waters is based on the ability of monovalent fluoride ions, the content of which in the chemical formula of, for example, Lazarevskaya healing mineral water reaches 3.5 mg / l as a specific component with a total salinity of 5.9-6, 1 mg / l, more actively than traditional drug regimens, to optimize not only the dynamics of the level of lipid peroxidation, but also the HCT test parameters according to Park, which determine the degree of biocidality of neutrophilic granulocytes s contained in the test blood volume (1 ml) of individuals exposed to harmful external influences, which are the cause of radionuclides, including the half-life of the active products of salts of heavy metals that enter the body of water or food. At the same time, the study of the immuno-hematopoietic (hematopoietic) regulatory function of the above individuals was based on methods of specifying the plasmin's fibrinolytic activity, blood tolerance to heparin, identification of such biochemical status constants as glutathione S-transferase (G-S-T) activity in saliva, alanine aminotransferase (ALT) and aspartate aminotransferase (ACT). In addition, the aforementioned properties of the natural halogen-containing mineral water “Lazarevskaya Healing” were confirmed by studying the dynamics of the phagocytic-cell defense parameters of macroorganisms, where a significant role was given to determining the phagocytic index, i.e. the percentage of actively phagocytic cells (phagocytes) and the phagocytic index, i.e. the average number of microbes absorbed by one phagocyte of individuals exposed to harmful external influences, which are caused by radionuclides, including the products of the active half-life of heavy metal salts that enter the body with water or food. The dynamics of a subpopulation of T cells carrying CD3 ⁺ surface antigens (mature T cells), CD4 ⁺ (helpers), CD8 ⁺ (suppressors), and serum IgA, IgM, IgG indices were determined by G. Mancini radial immunodiffusion using standard anti-immunoglobulin sera.

Based on this mechanism of regulation of indicators of biochemical and immuno-hematopoietic status of these individuals (n = 278, p <0.05), the authors first studied the effect (on individuals exposed to harmful external influences caused by radionuclides, including products of active half-life salts of heavy metals that enter the body with water or food) drinking balneotherapy using low-mineralized hydrocarbonate-chloride sodium, alkaline, boric, with a high content the use of magnesium, iodine and fluorine from the Lazarevskaya healing mineral water of well No. 84-E of the Volkonskoye deposit in the Sochi resort (see Lazarevskaya according to GOST R 54316-2011. Natural mineral drinking water. General specifications).

The prototype of the claimed invention is not. The analysis of the problem and the data of their own research allowed the authors to conclude the feasibility of removing radioactive substances from biological tissues by the claimed method of drinking balneotherapy using natural halogen-containing mineral water "Lazarevskaya healing."

Technical result

The technical result achieved by the claimed invention is to improve the health indicators of individuals exposed to harmful external influences, the cause of which are radionuclides, including the products of the active half-life of salts of heavy metals that enter the body with water or food products, i.e. normalization: 1) indicators of phagocytic cell defense of the body and a subpopulation of T cells carrying CD3 ⁺ surface antigens (mature T cells), CD4 ⁺ (helpers), CD8 ⁺ (suppressors), IgA, IgM, IgG serum and cytokines ( IFN-γ, IL-4) blood; 2) the level of lipid peroxidation products (in the heptane and isopropanol phase); 3) the basal and stimulated activity of neutrophilic granulocytes (indicators of the HCT test according to Park), their oxygen-independent biocide when calculating the average cytochemical coefficient (test EG Yablokova, 1999) [14]; 4) fibrinolytic activity of plasmin and blood tolerance to heparin; 5) the level of ALT (alanine aminotransferase) and aspartate aminotransferase (ACT).

Also the technical result is the fact that as a result of using the claimed author's complex of drinking balneotherapy, 86.3% of the observed individuals (n = 240, p <0.05) exposed to harmful external influences (which are caused by radionuclides, including products of the active half-life of salts heavy metals that enter the body with water or food) were classified as “practically healthy” according to the dynamics of their clinical, functional and laboratory parameters, i.e. in macroorganisms, the presence of the 99mTc - pertechnetate marker, which was used to determine using Toshiba gamma cameras to identify the level of accumulation of radioactive substances in biological tissues, was not observed.

SUMMARY OF THE INVENTION

The technical result is achieved by the fact that the method of removing radioactive substances from biological tissues involves the use of a halogen-containing hydrocarbonate-chloride sodium, alkaline, boric, with a high content of magnesium, iodine and fluorine of natural mineral water "Lazarevskaya healing" well No. 84-E of the Sochi Volkonsky field according to the following methodology: 30-35 minutes before meals in small sips, six times a day, 200-250 ml daily at t ° = (23-24) ° C, for 45 days, followed by a break of 2-3 days and a repeat of drinking the same volumes of the named natural halogen-containing mineral water over the next 45 days. The course of treatment includes a three-year period of drinking the use of natural mineral water "Lazarevskaya healing", and the specific time for daily use of this mineral water is determined at 8.00, 10.00, 12.00.14.00, 16.00, 18.00 hours.

Disclosure of invention

The inventive method includes scientifically based balneotherapy techniques for correcting the consequences of the negative impact of external influences, the cause of which are radionuclides, including products of the active half-life of salts of heavy metals that enter the body with water or food products.

The leading of these clinical and laboratory manifestations are: 1) a decrease in the level of phagocytic cell defense of the body and the destruction of a subpopulation of T cells carrying surface antigens CD3 ⁺ (mature T cells), CD4 ⁺ (helpers), CD8 ⁺ (suppressors), IgA , IgM, IgG serum and cytokines (IFN-γ, IL-4) blood; 2) the level of lipid peroxidation products (in the heptane and isopropanol phase); 3) the basal and stimulated activity of neutrophilic granulocytes (indicators of the HCT test according to Park), their oxygen-independent biocide when calculating the average cytochemical coefficient (test by EG Yablokova, 1999); 4) a decrease in the fibrinolytic activity of plasmin and blood tolerance to heparin; 5) increased levels of ALT (alanine aminotransferase) and aspartate aminotransferase (ACT).

The inventive method for removing radioactive substances from biological tissues includes the use of a halogen-containing hydrocarbonate-chloride sodium, alkaline, boric, with a high content of magnesium, iodine and fluorine of the Lazarevskaya healing water mineral water well No. 84-E of the Volkonskoye deposit in the Sochi resort according to the following method: 30-35 minutes before meals in small sips, six times a day daily, 200-250 ml at t ° = (23-24) ° C, for 45 days, followed by a break of 2-3 days and a repeat of drinking the same volumes named halogenated natural mineral water for the next 45 days. The course of treatment includes a three-year period of drinking the use of natural mineral water "Lazarevskaya healing", and the specific time for daily use of this mineral water is determined at 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 hours.

Drinking mineral water (certificate of state registration No. 77.99.19.6.U.11657.11.06 of 11/01/2006) with the trade name "Lazarevskaya healing" refers to the waters with low salinity of the Volkonsky or Solonikovsky deposits of mineral therapeutic and table water "Lazarevskaya" and is produced from sources:

b) well No. 84-E of the Volkonskoye field: mineral water of low salinity, sodium bicarbonate chloride, alkaline, boric, with a high fluorine content.

The formula of the ionic composition and other characteristics of the mineral water "Lazarevskaya healing" are shown in table 1.

The content of halogens (fluorine) in the chemical formula of the natural mineral water “Lazarevskaya Healing” reaches 3.0-3.5 mg / l as a specific component with a total mineralization of the water of 5.9-6.1 mg / l.

Table 1 No. Well., Field State Registration Name and Trade Name Formula of ionic composition Specificity, mg / dm ³ DEBIT, m ³ / day Using No. 84-E Volkonskoye "Lazarevskaya healing"

H ₃ BO ₃ 274.4 F3.5 569.5 Bottling plants, balneotherapy facilities (for shared baths, drinking and local procedures)

As mentioned above, the mechanism of action of halogen-containing natural mineral waters is based on the ability of monovalent fluoride ions more actively than traditional drug regimens to optimize not only the dynamics of the level of lipid peroxidation, but also the HCT test according to Park, which determine the degree of biocidality of neutrophilic granulocytes contained in test volume of blood (1 ml) of individuals exposed to harmful external influences, the cause of which are radionuclides, including products of the active half pada salts of heavy metals that enter the body with water or food.

The Federal Service for Supervision of Consumer Rights Protection and Human Well-Being (Rospotrebnadzor) signed by the chief sanitary doctor G.G. Onishchenko issued Certificate of State Registration No. 77.99.19.6. U.11657.11.06 of 01/01/2006 that the mineral water drinking medical and table "Lazarevskaya healing" (TU 9185-002-95093915-06) passed state registration, entered into The state register is also allowed for manufacturing on the territory of the Russian Federation, import and turnover on the territory of the Russian Federation (Appendix 2). This certificate was issued on the basis of the balneological conclusion of the Research Center of Balneology and Rehabilitation of the Black Sea Area Administration of the specialized sanatoriums of the Ministry of Health and Social Development of 08.08.06, No. 75, protocol of the AIL ITC "Quality" (No. ROSS RU.0001.21AU17) of 08.08.06, No. 1290. The validity period of this certificate of state registration is established by Rospotrebnadzor for the entire period of industrial production of Russian products or supplies of imported products (Certificate number 0037130).

In addition, the Federal Service for Supervision of Consumer Rights Protection and Human Well-being on Water, the Lazarevskaya Healing Mineral Drinking Medical-Dining-Table (TU 9185-002-95093915-06) issued the Sanitary and Epidemiological Conclusion No. 77.99.15.006.T.002079.11. 06 dated 01/01/2006 on the compliance of this mineral drinking medicinal-table water with the state sanitary and epidemiological rules and regulations of Sanitary and epidemiological regulations 2.3.2. 1078-01 "Hygienic requirements for safety and nutritional value of food products." This Sanitary and Epidemiological Conclusion is also signed by G.G. Onishchenko (Appendix 3).

Using the proposed method with the indicated drinking balneotherapy regimes allowed quarterly (with a follow-up period of 3 years a statistically significant number of individuals, n = 278 at p <0.05), normalization of the characteristics of the biochemical status and immune-hematopoietic (hematopoietic) regulatory function of the above individuals (subjected to harmful external influences, the cause of which are radionuclides, including the products of the active half-life of salts of heavy metals entering the body water or food products), namely: 1) the fibrinolytic activity of plasmin, blood tolerance to heparin, the activity of glutathione-S-transferase (G-ST) in saliva levels of alanine aminotransferase and aspartate aminotransferase (Table 2); 2) indicators of phagocytic cell defense of macroorganisms, i.e. the percentage of actively phagocytic cells (phagocytes) and the phagocytic index and the average number of microbes absorbed by a single phagocyte, the dynamics of a subpopulation of T cells carrying surface antigens CD3 ⁺ (mature T cells), CD4 ⁺ (helpers), CD8 ⁺ (suppressors), and the level of indicators of IgA, IgM, IgG serum and cytokines (IFN-γ, IL-4) blood (table 3); 3) the level of lipid peroxidation products (table 4) in the heptane and isopropanol phase; 4) basal and stimulated activity (table 5) of neutrophilic granulocytes (indicators of the HCT test according to Park), their oxygen-independent biocide when calculating the average cytochemical coefficient (test by EG Yablokova, 1999).

An illustration of the therapeutic effectiveness of the claimed method is the fact that as a result of using the method proposed by the authors for drinking balneotherapy on the research bases: CJSC Sanatorium Lazarevskoye (354200, Russia, Krasnodar Territory, Sochi, Sochi highway, 6; Act No. 40 of 01/28/2013); CJSC “Sanatorium“ Sea Star ”(354200, Russia, Sochi, Lazarevsky district, Pobedy St., 15; act of introduction No. 100 dated 01.24.2013), 86.3% of the observed individuals (n = 240, p <0.05), subjected to harmful external influences (the cause of which are radionuclides, including the products of the active half-life of salts of heavy metals that enter the body with water or food), were classified as “practically healthy” according to the dynamics of their clinical, functional and laboratory indicators, i.e. in macroorganisms, the presence of the 99mTc - pertechnetate marker, which was used to determine using the Toshiba GCA-90B gamma cameras to identify the level of accumulation of radioactive substances in biological tissues, was not observed. In the control group, where patients (n = 278, p <0.05) were treated on the same research bases (CJSC Sanatorium Lazarevskoye (354200, Russia, Krasnodar Territory, Sochi, Sochi Highway 6; act of introduction No. 40 dated January 28, 2013); CJSC "Sanatorium" Sea Star "(Sochi, Lazarevsky District, Victory Street 15, Sochi, 354200; introduction act No. 100 dated January 24, 2013), using standard methods of radiation therapy (sulfur-containing radioprotectors - modern derivatives of mercaptoethylamine, the radiation resistance of which by the mechanism of action is associated with the presence of a free SH-group; Indo ilaltilaminami, including serotonin, tryptamine, 5-methoxytryptamine; solkoserilom or serum globulins normal autoantibodies), the therapeutic efficacy was 1.5 times lower (as shown in Table 6).

The same serum globulins (with normal autoantibodies), administered intraperitoneally in the experiment under vivarium conditions to white mice, turned out to be less effective radioprotectors than the natural mineral halogen-containing water "Lazarevskaya healing" used to remove radioactive substances from biological tissues of mice. It should be emphasized that these radioactive substances were naturally administered to mice when they consumed milk taken by the method of unintentional selection of cows from the radioactive contamination zone that occurred as a result of the Chernobyl accident (with a level of 3.7 Bq / kg and higher in order to determine Sr ⁹⁰ and Cs ¹³⁷ ). In this case, the milk sample (weighing up to 1.5 kg) was acidified, evaporated on an electric stove and after being transferred to porcelain cups, it was carbonized to the stage of ashing in a muffle furnace at 400-500 ° C. Then, the cesium-137 content was determined in the filtrate, and the content of strontium-90 was determined in the precipitate of oxalates isolated from this sample. As a result of radiometric measurement, the following facts were revealed: 1) in the first group of laboratory mice, which were fed milk for 3 consecutive days, milk taken by unintentional selection of cows from the radioactive contamination zone that occurred as a result of the Chernobyl accident (level 3 , 7 Bq / kg and above), accelerated excretion of the above products of the half-life of salts of heavy metals from the body was noted, since mice of this group not only received an unlimited amount of halogen-containing protein in the diet native mineral water "Lazarevskaya healing", but also subjected to a forced dipping procedure in the above vessel with mineral water 3 times a day, i.e., 20 minutes or up to 1 hour daily. At the same time, the half-life of Cs ¹³⁷ was 29 days during radiometric measurement in the body of experimental mice, and Sr ^{90 was} 21 days from the moment of application of the above-described method of using natural halogen-containing mineral water “Lazarevskaya healing” in the form of a radioprotector. The observations were carried out at a statistically significant level (n = 278, p <0.05); 2) in the control group of laboratory mice, where sulfur-containing radioprotectors were added to the food of the observed animals (without using the Lazarevskaya healing mineral water), also against the background of the use of milk taken by the method of unintentional selection of cows from the radioactive infection zone resulting from of the accident at the Chernobyl nuclear power plant (with a level of 3.7 Bq / kg and above in order to determine Sr ⁹⁰ and Cs ^{137 in it} ), the rate of elimination of these half-lives of heavy metal salts from the body was as follows: strontium-90 was present in the biological tissues of mice for 30-32 days, and cesium-137 persisted for up to 1.5 months after three times daily drinking of this milk. The data from this laboratory experiment are also shown in table 6.

The hematopoietic (hematopoietic) regulatory system of observed individuals (n = 240, p <0.05) exposed to harmful external influences (which are caused by radionuclides, including products of the active half-life of heavy metal salts that enter the body with water or food), was evaluated according to indicators (table 2) of plasmin fibrinolytic activity, blood tolerance to heparin, glutathione S-transferase (G-ST) activity in saliva, alanine aminotransferase (ALT) and aspartate aminotransferase (ACT) levels.

table 2 Differences in biochemical status The main group (n = 278) Control gr (n = 278) before treatment after before treatment after Glutathione S-transferase (G-S-T) activity in saliva, N = 127-129 μmol / s g protein 188.4 ± 2.6 128.1 ± 1.5 190.4 ± 1.6 146.9 ± 1.8 ACT (N = 25-28 ME) 38.9 ± 0.4 26.7 ± 0.2 38.6 ± 0.3 32.4 ± 0.5 ALT (N = 28-31 ME) 39.8 ± 0.6 29.5 ± 0.3 39.4 ± 0.7 33.8 ± 0.1 Heparin Tolerance (N = 40-50 s) 73 ± 2 48 ± 1 73 ± 1 58 ± 2 Fibrinolytic activity of plasmin (N = 8-15 mm ² ) 6.2 ± 0.4 9.2 ± 0.1 6.3 ± 0.2 7.3 ± 0.1

Commenting on the data in Table 2, it should be pointed out that the initially deformed hematopoietic (hematopoietic) system parameters were regulated using the claimed method of drinking balneotherapy and had only a tendency to normalize under the influence of standard methods of radiation therapy with sulfur-containing radioprotectors - modern derivatives of mercaptoethylamine, whose radiation activity is associated by a mechanism with the presence of a free SH-group; indolylthylamines, including serotin, tryptamine, 5-methoxytryptamine; solcoseryl or serum globulins with normal autoantibodies. In addition, the claimed method ensured normalization in patients of the main observation group of alanine aminotransferase (from 39.8 ± 0.6 ME to 29.5 ± 0.3 ME at N = 28-31 ME) and aspartate aminotransferase (from 38.9 ± 0 , 4 ME to 26.7 ± 0.2 ME at N = 25-28 ME). In the control group, the ALT and ACT indicators after taking the above-mentioned pharmacological radioprotectors remained (respectively) at the level of 33.8 ± 0.1 ME and 32.4 ± 0.5 ME, which indicates the therapeutic effectiveness of the claimed method. At the same time, such a significant indicator of the biochemical status of the hematopoietic (hematopoietic) system, as the blood tolerance to heparin under the influence of the claimed method, returned to the level of 48 ± 1 s initially increased to 73 ± 2 s at N = 40-50 s. At the same time, the fibrinolytic activity of plasmin (N = 8-15 mm ² ), initially reduced in both observation groups to 6.2-6.3 mm ² , increased in patients from the main observation group to 9.2 ± 0.1 mm ² , remaining at the level of 7.3 ± 0.1 mm ² in patients from the control. The activity of glutathione-S-transferase (G-ST) in saliva at N = 127-129 μmol / s g of protein was in the patients of the main observation group (where the claimed method of drinking balneotherapy using the natural halogen-containing mineral water "Lazarevskaya healing") 128, 1 ± 1.5 μmol / s g of protein (initially 188.4 ± 2.6 μmol / s g of protein). A similar indicator in patients in the control group remained at the level of 146.9 ± 1.8 μmol / s g of protein, which was almost 14% higher than normal.

The dynamics of the phagocytic cell defense of the body and other characteristics of the immune status are presented in table 3.

Commenting on the data of Table 3, it should be indicated that initially the percentage of normal values of immune characteristics in the main observation group was of the total number of patients observed: a) by a phagocytic index of 58%; b) for the maturation of CD4 + lymphocytes 19.3%; c) on maturation of CD8 + lymphocytes 18.3%; g) for the activation of B-lymphocytes 21.1%; d) in terms of the normal cytostatistical effect of 22.2%. After the implementation of the claimed method, the percentage of normal values of the immune characteristics amounted to the total number of patients observed: a) according to the phagocytic indicator 93%; b) for the maturation of CD4 + lymphocytes 75.6%; c) maturation of CD8 + lymphocytes 71.3%; g) for the activation of B-lymphocytes 73.1%; d) in terms of the normal cytostatistical effect of 72.4%. In the control group, similar indicators were 1.3-1.5 times lower. Similar dynamics was observed in the main and control groups of the observed patients (before and after taking pharmacological or natural radioprotectors in the form of Lazarevskaya healing water mineral water), when the level of IgM immunoglobulin was initially reduced before treatment (1.41 ± 0.04 at N = 1, 59-1.63 g / l) normalized in patients of the main group, not reaching the norm threshold in patients from the control observation group, and initially increased IgG and IgA in the main observation group, respectively, decreased to 11.70 ± 0.04 g / l and 1.48 ± 0.03 g / l (never coming back to normal in patients receiving only medication radioprotectors). The latter was significantly (p <0.05) correlated with a decrease in the initially impaired characteristics of CD 11 b ⁺ , CD 20 ⁺ , CD 25 ⁺ , CD 95 ⁺ and HLA-DR ⁺ , which was achieved, including using the scientifically based claimed method of drinking balneotherapy using halogen-containing natural mineral water "Lazarevskaya healing".

The dynamics of the level of lipid peroxidation (in comparison with the effectiveness of drug and natural radioprotectors) are presented in table 4.

Table 4 FLOOR products in rel. units extinction The main group (n = 278) Control gr (n = 278) before treatment after before treatment after Heptane phase: E232 / 220-diene conjugates (N = 0.542-0.544) 0.734 ± 0.012 0.543 ± 0.001 0.729 ± 0.017 0.583 ± 0.010 E278 / 220-conjugated ketotrienes (N = 0.382-0.385) 0.449 ± 0.014 0.384 ± 0.001 0.446 ± 0.012 0.397 ± 0.001 E400 / 220-Schiff bases (N = 0.012-0.014) 0.028 ± 0.003 0.013 ± 0.001 0.029 ± 0.002 0.019 ± 0.001 Isopropanol phase: E232 / 220-diene conjugates (N = 0.445-0.448) 0.720 ± 0.022 0.446 ± 0.001 0.721 ± 0.017 0.498 ± 0.003 E278 / 220-conjugated ketotrienes (N = 0.275-0.277) 0.410 ± 0.024 0.276 ± 0.001 0.409 ± 0.025 0.283 ± 0.001 E400 / 220-Schiff bases (N = 0.047-0.049) 0.098 ± 0.005 0.048 ± 0.001 0.099 ± 0.003 0.064 ± 0.002

Commenting on the data of table 4, it should be emphasized that the claimed method of drinking balneotherapy is more effective than standard methods of radiation therapy, i.e. modern derivatives of mercaptoethylamine, 5-methoxytryptamine or serum globulins with normal autoantibodies influenced the normalization of E232 / 220-diene conjugates (N = 0.542-0.544) in the heptane phase from the initial level of 0.734 ± 0.012 to 0.543 ± 0.001 rel. units extinction. At the same time, diene conjugates in the isopropanol phase (N = 0.445-0.448) normalized in the main observation group from the initial level of 0.720 ± 0.022 to 0.446 ± 0.001, remaining at the level of 0.498 ± 0.003 rel. units extinction at the end of a course of drug radioprotectors. A similar dynamics was observed for other lipid peroxidation products, including conjugated ketotrienes and Schiff bases. The latter consisted of a direct correlation with the characteristics of the basal and stimulated activity of neutrophilic granulocytes, which are presented in table 5.

Table 5 Biocidity of neutrophilic granulocytes The main group (n = 278) Control gr (n = 278) before treatment after before treatment after 1. Park HCT test results: - basal activity of neutrophilic granulocytes (N = 10%) 8.1% 9.9% 8.2% 9.3% - stimulated activity of neutrophilic granulocytes (N = 40-60%) 29.8% 54.4% 29.9% 39.2% 2. Assessment of the content of lysosomal cationic proteins (test by EG Yablokov, 1999) when calculating the average cytochemical coefficient (SCC) of oxygen-independent biocide of neutrophilic granulocytes
(N = 1.5-1.7 srvc. Unit) 1.07 ± 0.04 conv. units 1.62 ± 0.03 conv. units 1,09 ± 0,05 srvc units 1.26 ± 0.02 srvc units

Commenting on the data of table 5, it should be emphasized that the claimed method allowed to normalize the indicator of oxygen-independent biocide of neutrophilic granulocytes (N = 1.5-1.7 srvc. Units), which (being initially reduced to 1.07 ± 0.04 srvc. .) after receiving the claimed method of using natural radioprotector in the form of mineral water "Lazarevskaya healing" rose to the level of 1.62 ± 0.03 srvc. units in patients of the main observation group. At the same time, in the main observation group, the basal activity of neutrophilic granulocytes increased to 9.9% (initially 8.1% at N = 10%), remaining in the control observation group at the level of 9.3%. The stimulated activity of neutrophilic granulocytes (N = 40-60%) in the main group was (after the implementation of the measures of the claimed method) 54.4%, remaining in the control group at 39.2%.

The final therapeutic effectiveness of the claimed method (when implementing the experimental part of its scientific justification) is presented in table 6.

Table 6 Experimental performance The main group (n = 278) Control gr (n = 278) before treatment after before treatment after 1. The level of dekorporation (excretion) from the body of the observed sick radionuclides penetrated naturally with water or food almost healthy 0% almost healthy 86.3% almost healthy 0% 55.4% are healthy products: (100 days) (100 days) Cs ¹³⁷ 45 days 70 days Sr ⁹⁰ 40 days 61 days 2. The level of dekorporation (excretion) from biological tissues of white mice of radionuclides that penetrated naturally with milk taken by the method of unintentional selection from cows from the radioactive contamination zone that occurred as a result of the Chernobyl accident (with a level of 3.7 Bq / kg and above) : 100 days 100 days Cs ¹³⁷ 29 days 44 days Sr ⁹⁰ 21 day 32 days

Commenting on the data in Table 6, it should be noted that in the first group of laboratory mice, which were fed milk for 3 consecutive days, milk taken by unintentional selection of cows from the radioactive contamination zone that occurred as a result of the Chernobyl accident (with a level of 3.7 Bq / kg and above), it was revealed after application of the inventive method that accelerated excretion of the aforementioned half-life products of heavy metal salts from the body. In this case, the half-life of Cs ¹³⁷ was 29 days during radiometric measurement in the body of experimental mice, and Sr ⁹⁰ - 21 days. In the control group, similar indicators were 1.5 times higher and amounted to 44 and 32 days, respectively. At the same time, the level of dekorporation (excretion) from the body of the observed sick radionuclides, penetrated naturally with water or food, under the influence of the claimed method was 1.5 times higher than standard methods of anti-radiation therapy with sulfur-containing radioprotectors or indolyl-amyl amines, including serotonin, tryptamine 5-methoxytryptamine. It was taken into account that according to the national Standard GOST R 54316-2011 (Mineral natural drinking water), the natural mineral halogen-containing water “Lazarevskaya Healing” with a total salinity of 5.0-6.0 g / dm ³ does not impose restrictions on the daily and exchange rate doses and, as part of its active biological components, boron, magnesium and fluoride ions are present in conditioned concentrations that contribute to the normalization of the physiological, physiological, immune and biochemical constants of individuals exposed to harmful external influences m, the cause of which are radionuclides, including the products of the active half-life of salts of heavy metals that enter the body with water or food.

Examples of the method

Example 1. Patient Sam-s, 36 years old, and / b No. 798/12, took a course of drinking balneotherapy according to the claimed method at CJSC Sanatorium Lazarevskoye (354200, Russia, Krasnodar Territory, Sochi, Sochi highway, 6) with December 4, 2012 to January 18, 2013 (45 days, the first course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing") and from January 23, 2013 to March 11, 2013 (45 days, the second course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing"). Diagnosis upon arrival in the spa card: adverse effects of industrial radiation (Z 57.1 according to ICD-X). From the anamnesis: the patient has been working as a commissioner of equipment at nuclear power plants for 10 years. Complaints of constant weakness (general malaise), decreased physical performance, drowsiness, increased bleeding from tissues with minor (domestic or industrial) damage to the skin surface, headache, especially towards evening, after physical exertion, irritability and conflict for minor reasons, relative decrease sexual potency. Is registered in the departmental health care institution at the place of production over the past 3 years for the syndromic combination of the above clinical and functional manifestations arising from occupational risk factors and classified by the International Statistical Classification of Diseases in Class XXI “Factors Affecting the Condition public health and appeals to health care institutions ”, which is officially classified in rubric Z 57.1 according to ICD-X with the wording“ N adverse effects of industrial radiation. " The treatment was carried out over the past 3 years at the place of deployment of the departmental healthcare institution (using standard methods of radiation therapy, including sulfur-containing radioprotectors - modern derivatives of mercaptoethylamine, the radiation resistance of which is associated with the presence of a free SH-group by the mechanism of action, and also indolyl-amyl amines, including serotonin, tryptamine, 5-methoxytryptamine). Due to the lack of the planned therapeutic effect, he was sent by the departmental health care institution to a course of drinking balneotherapy at ZAO Lazarevskoye Sanatorium (Sochi resort). Complaints upon admission: constant weakness (general malaise), decreased physical performance, drowsiness, increased bleeding from tissues with minor (domestic or industrial) damage to the skin surface, headache, especially towards evening, after physical exertion, irritability and conflict for minor reasons , relative decrease in sexual potency. Objectively: a patient of the correct physique, normal nutrition (with a height of 172 cm has a weight of 75.8 kg). The vesicular breathing, no wheezing. Heart sounds are rhythmic, somewhat muffled. Percussion: the limits of relative cardiac dullness are within normal limits, the tongue is moist, covered with a slight whitish bloom. The abdomen is soft, painless. Percussion border of the liver - along the edge of the costal arch. The chair is irregular (prone to constipation). Urination is free, painless, non-frequent. Pasternatsky’s symptom is negative on both sides. The color of the urine is straw yellow.

During a laboratory examination: a general blood test - hemoglobin 134 g / l, color 0.9, platelets 210 · 10 ⁹ , white blood cells 10.3 · 10 ⁹ , eos. 6, fell. 3, seg. 73, lymph. 25, mon. 10, ESR 18 mm / h; total an. urine - ud. weight 1012, clear full reaction acidic, there is no protein, there is no sugar, the epithelium is flat, a small amount, white blood cells 2-4 in n / a., no mucus.

Clinical and laboratory characteristics: glutathione S-transferase (G-ST) activity in saliva, N = 127-129 μmol / s g protein 188.4 ± 2.6 μmol / s g protein; ACT (N = 25-28 ME) 38.9 ± 0.4 ME; ALT (N = 28-31 ME) 39.8 ± 0.6 ME; blood tolerance to heparin (N = 40-50 s) 73 ± 2 s; fibrinolytic activity of plasmin (N = 8-15 mm ² ) 6.2 ± 0.4 mm ² ; Gender in rel. units extinctions: 1) heptane phase: E232 / 220-diene conjugates (N = 0.542-0.544) 0.734 ± 0.012; E278 / 220-conjugated ketotrienes (N = 0.382-0.385) 0.449 ± 0.014; E400 / 220-Schiff bases (N = 0.012-0.014) 0.028 ± 0.003 rel. units extinction; 2) isopropanol phase: E232 / 220-diene conjugates (N = 0.445-0.448) 0.720 ± 0.022; E278 / 220-conjugated ketotrienes (N = 0.275-0.277) 0.410 ± 0.024; E400 / 220-Schiff bases (N = 0.047-0.049) 0.098 ± 0.005 rel. units extinction. The percentage of normally phagocytic cells, i.e. phagocytic index (N = 92%), laboratory reduced to 58%, and phagocytic index, i.e. the average number of microbes absorbed by one phagocyte (N = 8-10) is reduced to 5. Blood cytokines: IFN-γ, pg / ml (N = 25-28) 34.26 ± 0.28; IL-4, pg / ml (N = 19-20) 36.10 ± 0.15; Th1 / Th2 (N = 1.4-1.5) 1.65 ± 0.02; IgM / g / l (N = 1.59-1.63) 1.41 ± 0.04; IgG, g / l (N = 11.2-11.9) 16.98 ± 0.21; IgA, g / l (N = 1.5) 2.18 ± 0.02; Other indicators of immune status upon admission: CD4 / CD8 (N = 1.98-1.99) 1.92 ± 0.02; CD16 ⁺ ,% (N = 11.1-11.4) 19.22 ± 0.11; CD25 ⁺ ,% (N = 6.17-6.19) 4.98 ± 0.26; HLA-DR ⁺ ,% (N = 12.4-12.6) 9.59 ± 0.41; CD95 ⁺ ,% (N = 24.1-24.3) 19.28 ± 0.16. Biocidity of neutrophilic granulocytes, including the results of the Park HCT test: basal activity of neutrophilic granulocytes (N = 10%) 8.1%; stimulated activity of neutrophilic granulocytes (N = 40-60%) 29.8%. Laboratory assessment of the content of lysosomal cationic proteins (test by E.G. Yablokov, 1999) when calculating the average cytochemical coefficient (SCC) of the oxygen-independent biocide of neutrophilic granulocytes (N = 1.5-1.7 conventional units) 1.07 ± 0.04 conv.ed

According to the anamnesis, clinical, laboratory, instrumental and functional studies, upon admission to the sanatorium the diagnosis was established: Adverse effects of industrial radiation (Z 57.1 according to ICD-X), which caused clinical and functional deformation (deterioration) of neutrophilic granulocyte biocidal indices, LPO characteristics, rheological properties blood, decreased activity of glutathione S-transferase (G-ST), phagocytic index and phagocytic index, maturation of CD4 ⁺ lymphocytes, CD8 ⁺ lymphocytes, activation of B lymphocytes.

Method of treatment: the author’s scheme for removing radioactive substances from biological tissues has been appointed, which includes the use of a halogen-containing hydrocarbonate-chloride sodium, alkaline, boric, with a high content of magnesium, iodine and fluorine of the Lazarevskaya healing mineral water of well No. 11-M of the Solonikovsky deposit in the Sochi resort according to the following method: 30 minutes before meals in small sips, six times a day, 200 ml daily at t ° = 23 ° C, for 45 days. The time of daily use of mineral water is at 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 hours. With a subsequent break of 2 days and a repeat of the drinking intake of the same volumes of the named natural halogen-containing mineral water for the next 45 days.

As a result of the treatment: there is a restoration of a sense of normal working capacity, the disappearance of increased bleeding with microtrauma, the normalization of the parameters of sexual life, a decrease in the level of irritability and conflict for minor reasons, the restoration of a regular (normal in consistency) stool.

During a laboratory examination: a general blood test - hemoglobin 124 g / l, color 1.0, platelets 209 · 10 ⁹ , white blood cells 6.1 · 10 ⁹ , eos. 2, fell. 1, seg. 61, lymph. 15, mon. 5, ESR 12 mm / h; total an. urine - ud. weight 1012, clear full reaction acidic, there is no protein, there is no sugar, the epithelium is flat, a small amount, white blood cells 2-4 in n / a., no mucus.

Clinical and laboratory characteristics: activity of glutathione-S-transferase (G-ST) in saliva, N = 127-129 μmol / s g protein 128/1 ± 1.5 μmol / s g protein; ACT (N = 25-28 ME) 26.7 ± 0.2 ME; ALT (N = 28-31 ME) 29.5 ± 0.3 ME; blood tolerance to heparin (N = 40-50 s) 48 ± 1 s; fibrinolytic activity of plasmin (N = 8-15 mm ² ) 9.2 ± 0.1 mm ² ; Gender in rel. units extinctions: 1) heptane phase: E232 / 220-diene conjugates (N = 0.542-0.544) 0.543 ± 0.01; E278 / 220-conjugated ketotrienes (N = 0.382-0.385) 0.384 ± 0.01; E400 / 220-Schiff bases (N = 0.012-0.014) 0.013 ± 0.01 rel. units extinction; 2) isopropanol phase: E232 / 220-diene conjugates (N = 0.445-0.448) 0.446 ± 0.01; E278 / 220-conjugated ketotrienes (N = 0.275-0.277) 0.276 ± 0.01; E400 / 220-Schiff bases (N = 0.047-0.049) 0.048 ± 0.01 rel. units extinction. The percentage of normally phagocytic cells, i.e. phagocytic index (N = 92%), laboratory increased to 93%, and the phagocytic index, i.e. the average number of microbes absorbed by one phagocyte (N = 8-10) increased to 9. Blood cytokines: IFN-γ, pg / ml (N = 25-28) 27.07 ± 0.02; IL-4, pg / ml (N = 19-20) 19.67 ± 0.03; Thl / Th2 (N = 1.4-1.5) 1/47 ± 0.01; IgM, g / l (N = 1.59-1.63) 1.62 ± 0.01; IgG, g / l (N = 11.2-11.9) 11.70 ± 0.04; IgA, g / l (N = 1.5) 1.48 ± 0.03. Other indicators of immune status upon admission: CD4 / CD8 (N = 1.98-1.99) 1.99 ± 0.01; CD16 ⁺ ,% (N = 11.1-11.4) 11.2 ± 0.09; CD25 ⁺ ,% (N = 6.17-6.19) 6.18 ± 0/01; HLA-DR ⁺ ,% (N = 12.4-12.6) 12.47 ± 0.02; CD95 ⁺ ,% (N = 24.1-24.3) 24.12 ± 0.02. Biocidity of neutrophilic granulocytes, including the results of the Park HCT test: basal activity of neutrophilic granulocytes (N = 10%) 9.9%; the stimulated activity of neutrophilic granulocytes (N = 40-60%) 54.4%. Laboratory assessment of the content of lysosomal cationic proteins (test by EG Yablokov, 1999) when calculating the average cytochemical coefficient (SCC) of the oxygen-independent biocide of neutrophilic granulocytes (N = 1.5-1.7 conv. Units) 1.62 ± 0.03 conv. units

Thus, according to the current criteria, sick Sam-s, 36 years old, and / b No. 798/12, who underwent a course of drinking balneotherapy with the claimed method at ZAO Lazarevskoye Sanatorium (354200, Russia, Krasnodar Territory, Sochi, Sochi highway, 6) from December 4, 2012 to January 18, 2013 (45 days, the first course of drinking balneotherapy using the natural mineral halogen-containing water "Lazarevskaya healing") and from January 23, 2013 to March 11, 2013 (45 days, the second course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing"), practically recognized as healthy ovym. The recommendation is a mandatory repeat of the treatment on a quarterly basis for a three-year period of drinking the use of natural mineral water “Lazarevskaya healing”, and the specific time for daily use of this mineral water is determined at 8.00, 10.00, 12.00.14.00, 16.00, 18.00 hours.

Example 2. Patient Rai-va, 34 years old, and / b No. 801/12, took a course of drinking balneotherapy according to the claimed method at ZAO Sanatorium Lazarevskoye (354200, Russia, Krasnodar Territory, Sochi, Sochi highway, 6) with 12/05/2012 to 01/19/2013 (45 days, 1st course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing") and from 01.24.2013 to 12.03.2013 (45 days, 2nd course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing"). Diagnosis upon arrival in the spa card: adverse effects of industrial radiation (Z 57.1 according to ICD-X). From the anamnesis: the patient has been working for 8 years as an operator of a technical control point for equipment at nuclear power plants. Complaints of constant weakness (general malaise), decreased physical performance, drowsiness, increased bleeding from tissues with minor (domestic or industrial) damage to the skin surface, headache, especially towards evening, after physical exertion, irritability and conflict for minor occasions, algdamenorrhea and irregularity (delay up to 5-8 days from the planned onset) of menstruation. Is registered in the departmental health care institution at the place of production over the past 4 years regarding the syndromic combination of the above clinical and functional manifestations arising from occupational risk factors and classified according to the International Statistical Classification of Diseases in Class XXI “Factors Affecting the Condition public health and appeals to health care institutions ”, which is officially classified in rubric Z 57.1 according to ICD-X with the wording“ N adverse effects of industrial radiation. " The treatment was carried out over the past 4 years at the place of deployment of the departmental healthcare institution (using standard methods of radiation therapy, including sulfur-containing radioprotectors - modern derivatives of mercaptoethylamine, the radiation protection of which is associated with the presence of a free SH-group by the mechanism of action, and also indolyl-amyl amines, including serotonin, tryptamine, 5-methoxytryptamine). Due to the lack of the planned therapeutic effect, she was sent by the departmental healthcare institution to a course of drinking balneotherapy at ZAO Lazarevskoye Sanatorium (Sochi resort). Complaints upon admission: constant weakness (general malaise), decreased physical performance, drowsiness, increased bleeding from tissues with minor (domestic or industrial) damage to the skin surface, headache, especially towards evening, after physical exertion, irritability and conflict for minor reasons , algdamenorrhea and irregularity (delay up to 5-8 days from the planned onset) of menstruation. Objectively: a patient of the correct physique, a somewhat reduced nutrition (with a growth of 158 cm has a weight of 54.1 kg). The vesicular breathing, no wheezing. Heart sounds are rhythmic, somewhat muffled. Percussion: the limits of relative cardiac dullness are within normal limits, the tongue is moist, covered with a slight whitish bloom. The abdomen is soft, painless. Percussion border of the liver - along the edge of the costal arch. The chair is irregular (prone to constipation). Urination is free, painless, non-frequent. Pasternatsky’s symptom is negative on both sides. The color of the urine is straw yellow.

During a laboratory examination: a general blood test - hemoglobin 129 g / l, color 0.9, platelets 212 · 10 ⁹ , white blood cells 12.4 · 10 ⁹ , eos. 5, fell. 5, seg. 72, lymph. 26, mon. 11, ESR 20 mm / h; total an. urine - ud. weight 1010, clear full reaction acidic, there is no protein, there is no sugar, the epithelium is flat, a small amount, leukocytes 2-3 p / zren., no mucus.

Clinical and laboratory characteristics; glutathione S-transferase (G-ST) activity in saliva, N = 127-129 μmol / s g of protein 186.2 ± 1.5 μmol / s g of protein; ACT (N = 25-28 ME) 38.5 ± 0.2 ME; ALT (N = 28-31 ME) 39.7 ± 0.3 ME; blood tolerance to heparin (N = 40-50 s) 71 ± 3 s; fibrinolytic activity of plasmin (N = 8-15 mm ² ) 6.1 ± 0.3 mm ² ; Gender in rel. units extinctions: 1) heptane phase: E232 / 220-diene conjugates (N = 0.542-0.544) 0.732 ± 0.009; E278 / 220-conjugated ketotrienes (N = 0.382-0.385) 0.448 ± 0.011; E400 / 220-Schiff bases (N = 0.012-0.014) 0.029 ± 0.004 rel. units extinction; 2) isopropanol phase: E232 / 220-diene conjugates (N = 0.445-0.448) 0.721 ± 0.023; E278 / 220-conjugated ketotrienes (N = 0.275-0.277) 0.409 ± 0.025; E400 / 220-Schiff bases (N = 0.047-0.049) 0.097 ± 0.002 rel. units extinction. The percentage of normally phagocytic cells, i.e. phagocytic index (N = 92%), laboratory reduced to 59%, and the phagocytic index, i.e. the average number of microbes absorbed by one phagocyte (N = 8-10) is reduced to 4. Blood cytokines: IFN-γ, pg / ml (N = 25-28) 34.22 ± 0.17; IL-4, pg / ml (N = 19-20) 36.08 ± 0.04; Th1 / Th2 (N = 1.4-1.5) 1.64 ± 0.01; IgM, g / l (N = 1.59-1.63) 1.42 ± 0.02; IgG, g / l (N = 11.2-11.9) 16.85 ± 0.12; IgA, g / l (N = 1.5) 2.16 ± 0.03. Other indicators of immune status upon admission: CD4 / CD8 (N = 1.98-1.99) 1.91 ± 0.01; CD16 ⁺ ,% (N = 11.1-11.4) 19.19 ± 0.05; CD25 ⁺ ,% (N = 6.17-6.19) 4.96 ± 0/13; HLA-DR ⁺ ,% (N = 12.4-12.6) 9.61 ± 0.32; CD95 ⁺ ,% (N = 24.1-24.3) 19.24 ± 0/05. Biocidity of neutrophilic granulocytes, including the results of the Park HCT test: basal activity of neutrophilic granulocytes (N = 10%) 8.2%; the stimulated activity of neutrophilic granulocytes (N = 40-60%) 30.1%. Laboratory assessment of the content of lysosomal cationic proteins (test by E.G. Yablokov, 1999) when calculating the average cytochemical coefficient (SCC) of the oxygen-independent biocide of neutrophilic granulocytes (N = 1.5-1.7 srvc. Units) 1.06 ± 0.06 conv. units

According to the anamnesis, clinical, laboratory, instrumental and functional studies, upon admission to the sanatorium the diagnosis was established: Adverse effects of industrial radiation (Z 57.1 according to ICD-X), which caused clinical and functional deformation (deterioration) of neutrophilic granulocyte biocidal indices, LPO characteristics, rheological properties blood, decreased activity of glutathione S-transferase (G-ST), phagocytic index and phagocytic index, maturation of CD4 ⁺ lymphocytes, CD8 ⁺ lymphocytes, activation of B lymphocytes.

Method of treatment: the author’s scheme for removing radioactive substances from biological tissues has been appointed, which includes the use of a halogen-containing hydrocarbonate-chloride sodium, alkaline, boric, with a high content of magnesium, iodine and fluorine of the Lazarevskaya healing mineral water of well No. 84-E of the Volkonsky deposit in the Sochi resort according to the following method: 35 minutes before meals in small sips, six times a day, 250 ml daily at t ° = 24 ° C, for 45 days. The time of daily use of mineral water is at 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 hours. With a subsequent break of 3 days and a repeat of the drinking intake of the same volumes of the named natural halogen-containing mineral water for the next 45 days.

As a result of the treatment: there is a restoration of a sense of normal performance, the disappearance of increased bleeding with microtrauma, the normalization of the regular periods of the onset of menstruation, a decrease in the level of irritability and conflict for minor reasons, the restoration of regular (normal in consistency) stool.

During a laboratory examination: a general blood test - hemoglobin 123 g / l, color 1.0, platelets 208 · 10 ⁹ , white blood cells 6.2 · 10 ⁹ , eos. 1, fallen. 2, seg. 56, lymph. 8, mon. 3, ESR 14 mm / h; total an. urine - ud. weight 1012, clear full reaction acidic, there is no protein, there is no sugar, the epithelium is flat, a small amount, leukocytes 2-3 p / zren., no mucus.

Clinical and laboratory characteristics: activity of glutathione-S-transferase (G-ST) in saliva, N = 127-129 μmol / s g protein 128.6 ± 0.2 μmol / s g protein; ACT (N = 25-28 ME) 26.6 ± 0.3 ME; ALT (N = 28-31 ME) 29.2 ± 0.4 ME; blood tolerance to heparin (N = 40-50 s) 47 ± 2 s; fibrinolytic activity of plasmin (N = 8-15 mm ² ) 9.6 ± 0.2 mm ² ; Gender in rel. units extinctions: 1) heptane phase: E232 / 220-diene conjugates (N = 0.542-0.544) 0.544 ± 0.01; E278 / 220-conjugated ketotrienes (N = 0.382-0.385) 0.383 ± 0.01; Е400 / 220-Schiff bases (N = 0.012-0.014) 0.014 ± 0.01 rel. units extinction; 2) isopropanol phase: E232 / 220-diene conjugates (N = 0.445-0.448) 0.447 ± 0.01; E278 / 220-conjugated ketotrienes (N = 0.275-0.277) 0.277 ± 0.01; Е400 / 220-Schiff bases (N = 0.047-0.049) 0.049 ± 0.01 rel. units extinction. The percentage of normally phagocytic cells, i.e. phagocytic index (N = 92%), laboratory increased to 91%, and the phagocytic index, i.e. the average number of microbes absorbed by one phagocyte (N = 8-10) increased to 8. Blood cytokines: IFN-γ, pg / ml (N = 25-28) 27.35 ± 0.04; IL-4, pg / ml (N = 19-20) 19.42 ± 0.02; Thl / Th2 (N = 1.4-1.5) 1.45 ± 0.01; IgM, g / l (N = 1.59-1.63) 1.61 ± 0.02; IgG, g / l (N = 11.2-11.9) 11.44 ± 0.03; IgA, g / l (N = 1.5) 1.49 ± 0.01. Other indicators of immune status upon admission: CD4 / CD8 (N = 1.98-1.99) 1.98 ± 0.02; CD16 ⁺ ,% (N = 11 / 1-11.4) 11.3 ± 0.03; CD25 ⁺ ,% (N = 6.17-6.19) 6.19 ± 0.01; HLA-DR ⁺ ,% (N = 12.4-12.6) 12.45 ± 0.01; CD95 ⁺ ,% (N = 24.1-24.3) 24.18 ± 0.03. Biocidity of neutrophilic granulocytes, including the results of the Park HCT test: basal activity of neutrophilic granulocytes (N = 10%) 9.8%; the stimulated activity of neutrophilic granulocytes (N = 40-60%) 51.6%. Laboratory assessment of the content of lysosomal cationic proteins (test by EG Yablokova, 1999) when calculating the average cytochemical coefficient (SCC) of the oxygen-independent biocide of neutrophilic granulocytes (N = 1.5-1.7 conv. Units) 1.61 ± 0.04 conv. units

Thus, according to the current criteria, a patient of Ra-va, 34 years old, and / b No. 801/12, who underwent a course of drinking balneotherapy with the claimed method at ZAO Lazarevskoye Sanatorium (354200, Russia, Krasnodar Territory, Sochi, Sochi highway, 6) from 12/05/2012 to 01/19/2013 (45 days, the first course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing") and from 01/24/2013 to 03/12/2013 (45 days, the 2nd course of drinking balneotherapy using natural mineral halogen-containing water "Lazarevskaya healing"), practically recognized as fucking. The recommendation is a mandatory repeat of the treatment on a quarterly basis for a three-year period of drinking the use of natural mineral water “Lazarevskaya healing”, and the specific time for daily use of this mineral water is determined at 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 hours.

Example 3. In the framework of the experiment, radioactive substances were naturally introduced to white laboratory mice (n _total = 556) when they consumed milk taken by unintentional selection of cows from the radioactive contamination zone that occurred as a result of the Chernobyl accident (with a level of 3.7 Bq / kg and above in order to determine in it Sr ⁹⁰ and Cs ¹³⁷ ). In this case, the milk sample (weighing up to 1.5 kg) was acidified, evaporated on an electric stove and after being transferred to porcelain cups, it was carbonized to the stage of ashing in a muffle furnace at 400-500 ° C. Then, the content of cesium-137 was determined in the filtrate, and the content of strontium-90 was determined in the precipitate of oxalates extracted from this sample. As a result of radiometric measurement, the following facts were revealed: 1) in the first group of laboratory mice (n = 278, p <0.05), which were fed milk for 3 consecutive days, taken by unintentional selection from cows from the radioactive infection zone, occurred as a result of the accident at the Chernobyl nuclear power plant (with a level of 3.7 Bq / kg and above), there was an acceleration of excretion of the above products of the half-life of salts of heavy metals from the body, since mice of this group not only received an unlimited amount of halogenoder in the diet burning natural mineral water "Lazarevskaya healing", but also underwent a forced dipping procedure in a container with the above mineral water 3 times a day, i.e. 20 minutes or up to 1 hour daily. At the same time, the half-life of Cs ¹³⁷ was 29 days during radiometric measurement in the body of experimental mice, and Sr ⁹⁰ - 21 days from the moment of application of the above-described method of using natural halogen-containing mineral water “Lazarevskaya healing” in the form of a radioprotector; 2) in the control group (n = 278, p <0.05) of laboratory mice, where sulfur-containing radioprotectors were added to the food of the observed animals (without using Lazarevskaya healing mineral water), also against the background of the use of milk taken by unintentional selection in cows from the zone of radioactive contamination that occurred as a result of the accident at the Chernobyl nuclear power plant (with a level of 3.7 Bq / kg and above in order to determine Sr ⁹⁰ and Cs ^{137 in it} ), the rate of excretion of these products of the half-life of heavy metal salts from the body turned out to be eating: strontium-90 was present in the biological tissues of mice for 30-32 days, and cesium-137 persisted for up to 1.5 months after three times daily drinking of this milk.

The data presented allow us to conclude that the proposed method for removing radioactive substances from biological tissues using drinking mineral water with the trade name "Lazarevskaya Healing" is effective. For individuals exposed to the adverse effects of radiation, the recommended method can be used at the stage of outpatient treatment both in outpatient and in sanatoriums.

INFORMATION SOURCES

1. Patent of the Russian Federation No. 2063229. Means for the excretion of radionuclides deposited in the body (published: 07/10/1996).

2. RF patent No. 113228. A method of accelerating the elimination of radioactive substances, mainly cesium-137 and its analogues from the human body (published: 06/20/1998).

Appendix No. 1

Bibliography

1. Cesium-137. Definition in foods. Methodical instructions of the Ministry of Health of the Russian Federation No. 5779-91. - M .: Medicine, 1991 .-- 142 p.

2. The basic sanitary rules for ensuring radiation safety (OSPORB-99) .SP 2.6.1. 799-99. - M .: RIO Ministry of Health of the Russian Federation, 2000 .-- 148 p.

3. Cesium-137. Control methods. Physical factors. Determination of specific activity in food products. Guidelines (MUK 4.3.2504-09) .- Federal Medical Biophysical Center. A.I. Burnazyan, 2009 .-- 79 p.

4. Ovoshnikova L.V., Koryagin A.S., Eliseeva A.A. The effect of EHF radiation on the blood system and lipid peroxidation in experimental radiation sickness // Bulletin of the Nizhny Novgorod State University. N.I. Lobachevsky. - No. 2. - 2001.- P.31-36.

5. Komochkov M.M., Lebedev V.N. A practical guide to radiation safety on charged particle accelerators. Vol. 3. - M .: Publishing house "Energoizdat", 2001. - 294 p.

6. Kozlov V.F. Radiation Safety Handbook. Ed. 6th - M .: Publishing house "Energoatomizdat", 2002. - 318 p.

7. Mashkovich V.P., Kudryavtseva F.V. Protection against ionizing radiation. Directory. Ed. 5th. - M .: Publishing house "Energoatomizdat", 2000. - 182 p.

8. Tutelian V.A. et al. The methodology of liberalized diet therapy for people exposed to harmful external influences of radiation // Modern diet therapy: Materials IV All-Russian. Conf. - Nizhny Novgorod, 2002. - S.4-8.

9. Guidelines “Determination of individual effective and equivalent doses and organization of control of occupational exposure under controlled conditions of treatment with radiation sources. General requirements ”МУ 2.6.1.016-2000. - M.: RIO Rosatom, 2000 .-- 226 p.

10. Ilyin L.A., Kirillov V.F., Korenkov I.P. Radiation safety and protection. M .: Medicine, 1996 .-- 296 p.

11. Morey A.N., Zykova A.S. Guidelines for sanitary control of the content of radioactive substances in environmental objects. - M.: Atomizdat, 1980 .-- 336 p.

12. Yablokov EG Determination of oxygen-independent biocide in the calculation of the average cytochemical coefficient // Laboratory research. - 1999. - No. 2. - S. 82-86.

Claims (2)

1. The method of removing radioactive substances from biological tissues involves the use of a halogen-containing hydrocarbonate-chloride sodium, alkaline, boric, with a high content of magnesium, iodine and fluorine of the Lazarevskaya healing water mineral water of well No. 84-E of the Volkonskoye deposit in the resort of Sochi according to the following method: 30-35 minutes before meals in small sips, six times a day daily, 200-250 ml at t ° = (23-24) ° C, for 45 days, followed by a break of 2-3 days and a repeat of drinking the same volumes named natural halo ensoderzhaschey mineral water over the next 45 days, the treatment includes the application of a three drinking natural mineral water "Lazarevskaya healing." 2. The method according to claim 1, including daily use of mineral water at 8.00, 10.00, 12.00, 14.00, 16.00, 18.00 hours.