RU2499771C1 - Water disinfectant - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: water disinfectant contains a polyguanidine compound - poly-(4,9-dioxadodecane guanidine) phosphate or poly-(4,9- dioxadodecane guanidine) chloride or poly-(4,9- dioxadodecane guanidine) gluconate or poly-(4,9- dioxadodecane guanidine) citrate or poly-(4,9- dioxadodecane guanidine) benzoate, or polyhexamethylene guanidine citrate or polyhexamethylene guanidine gluconate or polyhexamethylene guanidine benzoate or polyhexamethylene guanidine phosphate or polyhexamethylene guanidine chloride; hydroxyethyl cellulose, guanidine hydrochloride or water, with the following ratio of components in wt %: polyguanidine compound - 0.5-8.0; hydroxyethyl cellulose - 0.1-2.0; guanidine hydrochloride - 0.001-0.02; water- the balance.

EFFECT: invention increases efficiency of disinfecting water, reduces toxic properties of the disinfectant, including allergic activity.

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Description

The invention relates to the field of sanitation and hygiene, and in particular to disinfectants for various types of water, in particular drinking water, municipal and industrial wastewater, swimming pool water and equipment cooling systems. As well as protecting pipelines and structures from pathogenic bacteria and biological fouling.

Currently, there are a large number of physical and physico-chemical methods of disinfecting water.

The most widespread chemical treatment of water using liquid chlorine, bleach and sodium hypochlorite.

However, the use of chloragents (guidelines for preventive disinfection No. 28-2 / 6 of the Ministry of Health of the USSR, M, 1980) requires special precautions during storage and dosage.

In addition, chloragent, combining with organic substances, forms carcinogenic compounds in water.

Closest to the proposed solution is a composition for disinfection, including polyguanidine compounds and Quaternary ammonium compounds (HOUR) (US Pat. RF No. 2372943 class. A61L 2/18, 2008).

However, the HOURs included in the known composition exhibit a relatively narrow spectrum of antimicrobial activity - they are effective against pathogens of intestinal and drip infections of bacterial etiology, but are insufficiently active against Proteus vulgarism, Proteus mordani cultures, which significantly limits the possibilities of their use. In addition, HOUR is an allergen.

The known composition contains the following components, wt.%

Polyguanidine Compound 0.1-8.0 Synergistic Quaternary Ammonium Mixture compounds 0.25-24.0 Water rest

The technical problem solved by this invention is to increase the degree of effectiveness of disinfection of water, reduce the toxic properties of the product, including allergic activity.

To solve the technical problem, a composition for disinfecting water, including a polyguanidine (PG) compound - poly- (4,9-dioxadodecanguanidine) phosphate (PDDG), or poly- (4,9-dioxadodecanguanidine) chloride, or poly- gluconate (4.9 -dioksadodekanguanidina) citrate or poly (4,9-dioksadodekanguanidina) benzoate or poly (4,9-dioksadodekanguanidina) or polyhexamethylene guanidine citrate (PHMG) or polyhexamethylene guanidine gluconate, benzoate or polyhexamethylene guanidine or polyhexamethylene guanidine phosphate and polyhexamethylene guanidine chloride and water additional notably contains hydroxyethyl cellulose and guanidine hydrochloride in the following ratio, wt.%.

Polyguanidine Compound 0.5-8.0 Hydroxyethyl cellulose 0.1-2.0 Guanidine hydrochloride 0.001-0.02 Water rest

Hydroxyethyl cellulose is a white, amorphous solid that dissolves well in water. Water-soluble polymer {C ₆ H ₇ O ₂ (OH) ₃ (OCH ₂ CH ₂ ) _y OH _x } _n . In aqueous solutions, compatible with chlorides, nitrates and carbonates.

Hydroxyethyl cellulose (SCE) has a flocculating effect, but this substance is subject to active biodegradation. Together with the polyguanidine compound, the SCE can retain its flocculating properties for a long time. Use SCE manufactured by ShinEtsu SE Tylose GMBH Co., KG.

Disinfection of the proposed tool is based on the bactericidal activity of guanidine groups. The mechanism of the bactericidal action of polyguanidine compounds on microorganisms can be represented as follows:

a) guanidine polycations are adsorbed on a negatively charged surface of a bacterial cell, thereby blocking respiration, nutrition, and metabolite transport through the bacterial cell wall;

b) PG macromolecules diffuse through the cell wall, causing irreversible structural damage at the level of the cytoplasmic membrane, nucleotide, cytoplasm;

c) PGs bind to acid phospholipids, proteins of the cytoplasmic membrane, which leads to its rupture.

d) the result of this is the blockade of glycolytic enzymes of the respiratory system, the loss of pathogenic properties and the death of a microbial cell.

The polyguanidine compound causes the death of gram-positive and gram-negative microorganisms.

As a result of tests, it was found that the disinfecting and flocculating effect of the polymer in the aquatic environment, as well as its hygienic safety for the warm-blooded organism with prolonged use, provide the possibility of efficient use of polyguanidine compounds in almost all areas of water treatment. The reagent can be used for purification and disinfection of drinking water, urban and industrial wastewater, swimming pool water and equipment cooling systems, hot water of open heat supply systems, as well as protecting pipelines and structures from pathogenic bacteria and biological fouling. At the same time, the necessary technological effect is achieved and at the same time an improvement in water quality is observed. The polyguanidine compound is a well-soluble synthetic organic polymer in water. It is not volatile, does not give the smell and color to water.

The polyguanidine compound exhibits the properties of highly stable chemicals in the aquatic environment and has a prolonged bactericidal effect. After 20 days of observation, polyguanidine was detected in water at almost initially given concentrations, and a repeated foci of contamination (within the initial values of the microbial load) introduced into the once-disinfected water disappeared within 1 h without any addition of polyguanidine.

Polyguanidine compounds manufactured by the Institute of Ecological and Technological Problems (ROO IETP).

The flocculating effect of PG is based on the polarity of the guanidine group of the polymer, which has a positive charge and gives PG the properties of a cationic flocculant. The addition of a polyelectrolyte to water in concentrations typical for the flocculant leads to the enlargement of pollution particles and a decrease in their number. This occurs as a result of mutual adhesion of particles and confirms the presence of flocculating properties of the reagent.

Guanidine hydrochloride (GHC) is a crystalline powder. It is a good disinfectant, as well as denatured protein. GHC interacts with PG and a joint effect on the bacterial cell occurs. As a result, a developed system of biocidal action is created, which includes GHC, a combination of polyguanidine and HEC.

It is assumed that the SCE as a nonionic surfactant interacts with the surface of the microbial cell and promotes adsorption on it. GHC and PG act on the bacterial cell together. Presumably, the GHG connection, destroying the membrane of the microbial cell, facilitates the penetration of GHC into it, which denatures proteins and accelerates cell death.

The combined effect of GHC, HEC and polyguanidine polycations has a stronger bactericidal effect than polyguanidine alone. In addition, the spectrum of antimicrobial activity is expanding significantly, and, due to the prolonged action, the shelf life of the antimicrobial action increases several times.

The composition is prepared as follows. In the flask with a stirrer containing water, add SCE, stirring until a homogeneous mixture is formed.

In a separate flask with a stirrer, prepare an aqueous solution of a compound of polyguanidine and GHC. To the resulting solution of the HEC mixture is added a solution of the polyguanidine compound, GHC and water to a predetermined concentration.

The following examples illustrate the invention.

Example 1

47 ml of water are poured into two flasks with stirrers. The first is loaded, constantly mixing, polyhexamethylene guanidine (PHMG) hydrochloride in the amount of Zgi GHC in the amount of 0.02 g. In the second flask is placed 1 g of SCE. After complete dissolution, a solution of the second flask is added to the solution of the first flask, receiving 100 ml of the following disinfectant: 3% PHMG hydrochloride, 1% HEC, 0.02% GHC.

This composition can be used to disinfect various types of water, for example, when bringing its indicators to the quality of drinking water, taking into account the established MPC for the components of the disinfectant.

Other examples are summarized in table No. 1. The ratio of the components in the composition is the optimal value and identified as a result of numerous experiments. The data in table No. 1 confirm the optimality of the declared limits.

Alternative features of the claims provide the same technical result as shown in table No. 1.

Samples of the drugs were tested for antimicrobial activity. Minimum inhibitory concentrations (MICs) were determined for bacteria of the species Pseudomonas aeruginosa and molds of the species Aspergillus niger using serial dilutions in liquid media.

As a liquid nutrient medium for the cultivation of bacteria of the species Pseudomonas Aeruginosa, trypcase-soy broth (TSB) from bioMerieux, France, was used to grow Aspergillus Niger, a moldy liquid medium, екаapek liquid.

Seventeen sterile tubes were poured into 2 ml of a liquid nutrient medium. 2 ml of stock solution was added to the first tube. The contents were mixed and 2 ml was transferred to a second tube and so on to the 15th tube, from which 2 mm was removed. The contents of the 16th test tube served as a control of the growth of microorganisms, and the 17th control of the sterility of the nutrient medium. In all tubes except the 17th, 0.2 ml of culture was added a test of the microorganism.

Crops with bacterial cultures were incubated in an incubator at 37 ° C for 18-24 hours, and with mold cultures at 29 ° C, 10-14 days. The results were taken into account in the presence of the growth of microorganisms in the control culture and the absence in the control environment. Then the last tube was noted with a complete visible growth retardation of microorganisms. This dilution was the minimum inhibitory concentration for the test strain and determined the degree of its bacteriostatic activity to this agent.

To establish the bactericidal activity of all “not grown” (that is, which did not show visible growth of test microorganisms) tubes with liquid nutrient media and two “germinated” (as a control) using a bacteriological loop, seeding was performed on solid nutrient media. Crops with bacterial cultures were incubated at 37 ° C for 24-72 hours, and with fungal cultures at 29 ° C for 10-14 days.

The last dilution of the agent in a nutrient medium, from which it was not possible to obtain viable cells of the tested microorganisms, was considered bactericidal. The results for determining the minimum inhibitory concentration of disinfectants are presented in tables 2 and 3.

Table 1 Examples illustrating the invention No. Polyguanidine Compound SCE GHC Water Technical result (wt.%) Connection Name one 3.0 PHMG Chloride 1,0 0.02 Rest 2 8.0 Phosphate PGMG 2.0 0.001 Rest 3 2.0 PGMG benzoate 0.1 0.015 Rest four 0.5 PHMG Citrate 1,0 0.018 Rest 5 1,0 Gluconate PGMG 0.2 0.001 Rest The composition has a wide range of biocidal activity 6 0.5 PDDG Chloride 0.5 0.02 Rest 7 1,0 PDH phosphate 1,0 0.001 Rest 8 2.0 PDOH benzoate 2.0 0.015 Rest 9 3.0 PDG Citrate 2.0 0.02 Rest 10 2.0 Gluconate PDG 1,0 0.02 Rest eleven 8.05 * PHMG Chloride 1,0 0.015 Rest Component consumption unreasonably high 12 0.3 * PHMG Chloride 1,0 0.001 Rest Decreased bacterial activity 13 1,5 PHMG Chloride 0.058 * 0.01 Rest Decrease in biocidal action of guanidine component fourteen 1,5 PHMG Chloride 2.05 * 0.01 Rest Deterioration of technological properties of the solution fifteen 1.5 PHMG Chloride 1,0 0,0005 * Rest Decrease in biocidal action 16 1,5 PHMG Chloride 1,0 0.022 * Rest Change in biocidal action * - examples beyond the stated limits

table 2 Comparative data on minimally inhibitory concentrations of disinfectant compounds against bacteria of the P.aeruginosa species The composition of the disinfectant The total concentration in water of all substances that make up the disinfectant,% 0.007 ± 0.0003 0.0035 ± 0.0003 0.002 ± 0.0003 0.001 ± 0.0003 Prototype (Example No. 1) + - - - Example No. 1 + + + - Example No. 2 + + + - Example No. 3 + + + - The sign "+" marks the concentration that has a bactericidal effect The “-" sign indicates a concentration that does not have a bactericidal effect.

Table 3 Comparative data on the minimum inhibitory concentrations of disinfecting compounds against bacteria of the species Aspergillus niger The composition of the disinfectant The total concentration in water of all substances that make up the disinfectant,% 0.1 ± 0.03 0.05 ± 0.003 0.025 ± 0.0003 0.012 ± 0.0003 Prototype (Example No. 1) + - - - Example No. 1 + + + - Example No. 2 + + + - Example No. 3 + + + - The sign "+" marks the concentration that has a bactericidal effect The “-" sign indicates a concentration that does not have a bactericidal effect.

Claims (1)

A disinfectant for disinfecting water, comprising a poly- (4,9-dioxadodecanguanidine) polyguanidine-phosphate compound, or poly- (4,9-dioxadodecanguanidine) chloride, or poly- (4,9-dioxadodecanguanidine) gluconate, or poly- ( 4,9-dioxadodecanguanidine), or poly- (4,9-dioxadodecanguanidine) benzoate, or polyhexamethylene guanidine citrate, or polyhexamethylene guanidine gluconate, or polyhexamethylene guanidine benzoate, or polyhexamethylene guanidine phosphate, or the addition of polyhexamethylene guanidine phosphate or tion comprises hydroxyethylcellulose guanidine hydrochloride and the following component ratio, wt.%:
polyguanidine compound 0.5-8.0 hydroxyethyl cellulose 0.1-2.0 guanidine hydrochloride 0.001-0.02 water rest