RU2485963C1 - Method for preparing hemostatic product 'gelplastan' - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to medical pharmacology and represents a method for preparing a hemostatic product, involving treatment of gelatin in distilled water, incubation, cooling, addition of blood plasma and an antibiotic to gelatin, mixing, cooling, hardening, washing of an end product in water at least twice, grinding, drying at temperature 90°-200°C, additional grinding and sterilisation of the target product; cooling preceding hardening is conducted in a cold room at temperature -3°--5°C for 2.5 hours; the hard mass is carved 2.0×2.0×2.0 cm. The hemostatic may be used to arrest parenchymal and capillary hemorrhages in surgical practice.

EFFECT: invention provides reducing power consumption and enhancing work capacity with keeping the production process continuous.

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Description

The invention relates to medical pharmacology, and in particular to methods for producing organic hemostatic agents used to stop parenchymal and capillary bleeding in surgical practice.

It is known hemostatic agent "Zhelplastan", upon receipt of which dry gelatin is mixed with distilled water, the resulting mass is heated to a predetermined temperature and maintained until the hydrolysis process is completed, cooled to a predetermined temperature, dry blood plasma is mixed with an antibiotic, dissolved in distilled water, the resulting mass is mixed with the aforementioned gelatin melt cooled to a predetermined temperature to a homogeneous state, cool the resulting mass to a jelly-like state, produce oak pouring the mass and washing with water, grind the mass, dry, grind to a powder state, pack and sterilize (USSR Patent N 725289, IPC A61K 35/14, published on July 15, 1981).

However, this method is characterized by a lengthy process of preparing the product.

The closest technical solution to the proposed one is a method for producing a hemostatic agent "Zhelplastan", which includes treating gelatin with distilled water, incubation, cooling, adding blood plasma and antibiotic to gelatin, mixing, cooling, tanning, after which the target product is washed with water at least twice , grinding, drying at +90 - + 200 ° C, additional grinding with subsequent sterilization of the target product (RF Patent No. 2067447, IPC A61K 35/14, publ. 10.10.1996).

The disadvantage of this method is that the process of cooling a mixture of molten gelatin and a solution of blood plasma of cattle with an antibiotic before tanning and the process of tanning a large mass of the product takes a long time, which in turn leads to an increase in energy consumption and insufficient productivity.

The problem to which the invention is directed, is to reduce energy consumption and increase productivity while observing the continuity of the process.

This is achieved by the fact that in the method for producing a hemostatic agent, comprising treating gelatin with distilled water, incubation, cooling, adding blood plasma and antibiotic to gelatin, mixing, cooling, tanning, washing the target product with water at least two times, grinding, drying at a temperature of 90 -200 ° C, additional grinding followed by sterilization of the target product, according to the invention, the cooling before tanning is carried out in a refrigerator at a temperature of -3 - -5 ° C for 2.5 hours, then solidified th mass is cut into pieces 2.0 × 2.0 × 2.0 cm.

The invention is illustrated by the following example.

Dry edible gelatin is poured into a container, filled with distilled water in the ratio: 0.8-4.0 volumes of water per gelatin volume and incubated for 1-2 hours. Swollen gelatin is melted at 90-120 ° C and kept at this temperature to completion of the hydrolysis process. The molten gelatin is cooled to 32-42 ° C. Freeze-dried cattle blood plasma is mixed with an antibiotic, for example kanamycin sulfate, and filled with distilled water in the ratio: 5 volumes of water per volume of a mixture of blood plasma and antibiotic. Stir until completely dissolved. The prepared solution of cattle blood plasma with antibiotic is poured into cooled molten gelatin and thoroughly mixed until a homogeneous state.

The resulting mixture is cooled in a refrigerator for 2.5 hours at a temperature of -3 ° - -5 ° C until a hardened mass is obtained. The hardened yellow plate mass is cut into cubes 2.0 × 2.0 × 2.0 cm, which are placed in a solution of formaldehyde of 40% concentration with a temperature of 10 ° C and tanned until a dense cartilaginous state is obtained. The purged mass is poured with distilled water until the mass is completely immersed in water and incubated for 2 hours, then the water is drained, refilled with distilled water and incubated for another 2 hours, then the water is drained and the washing operation is repeated a third time. Grind to obtain granules. Dried at 90-200 ° C until a residual moisture content of 7% is obtained. Dry granules of "zhelplastan" are ground in a mill to a powder state, after which they are Packed in bottles and sterilized at + 80 ° C-120 ° C for 2 hours.

The predetermined temperature regime and time parameter during cooling to tanning of the yellow plate mass were established empirically to obtain a convenient consistency when cutting the cooled mass into cubes, which subsequently do not tend to stick together.

The sizes of the cubes are selected from the conditions for uniform penetration of formalin when tanning throughout the entire volume of the cube for the time established by the industrial regulations.

The combination of new essential features allows to ensure a continuous technological process, saving energy resources and increasing the productivity of the industrial production of the drug "Zhelplastan".

Claims (1)

A method of obtaining a hemostatic agent, including treating gelatin with distilled water, incubation, cooling, adding blood plasma and an antibiotic to gelatin, mixing, cooling, tanning, washing the target product with water at least two times, grinding, drying at a temperature of 90 ÷ 200 ° C, additional grinding with subsequent sterilization of the target product, characterized in that the cooling before tanning is carried out in a refrigeration chamber at a temperature of -3 ° ÷ 5 ° C for 2.5 hours, then the hardened mass is cut into portions 2.0 × 2.0 × 2.0 cm.