RU2472154C2 - Liquid of diagnostics of coccidia oocysts, cysts of balantidium coli and giardia, helminth eggs of different classes, mites, insects, separate stages of their development - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: liquid contains the following components: the first component comprising water - 48% and sodium chloride - 25%, the second component comprising sodium lauryl sulfate - 16.3%, saturated salt solution, polyethylene glycol - 10% and sodium benzoate - 0.7%. The first and second components are mixed together in a ratio of 1:1. The liquid is homogeneous, opalescent, translucent, of syrupy consistency.

EFFECT: liquid in the proposed composition is not toxic to humans, does not require special storage conditions, increases the range of the defined diseases.

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Description

The invention relates to veterinary medicine, medicine and is intended to detect coccidia oocysts, cysts of balantidia and giardia (giardia), helminth eggs of different classes (nematodes, trematodes and cestodes), ticks, insects, their individual stages of development from scrapings from animals, birds, from substrates , from environmental objects and fecal masses, by their deposition and flotation.

Known solutions for detecting oocysts of protozoa and helminth eggs: a saturated solution of sodium chloride NaCl; ammonium nitrate solution - ammonium nitrate NH ₄ NO ₃ ; Darling liquid (prototype), which is a mixture of glycerol and a saturated solution of NaCl salt in a ratio of 1: 1. (1. Practical parasitology, under the editorship of prof. D.V. Vinogradov-Volzhinsky L. 1977; 2. Handbook of a veterinary laboratory assistant under the editorship of V.Ya. Antonov, M., Kolos, 1981; 3. Kotelnikov G.A. Helminthological studies of animals and the environment. Handbook, M., 1984; 4. Workshop on the diagnosis of invasive diseases of farm animals. Edited by M.Sh. Akbaev. M., Kolos, 1994; 5. Larionov SV, Davydov Yu .M., Bychkova L.V. Workshop on Parasitology. Saratov, Federal State Educational Institution of Higher Professional Education "Saratov State Autonomous Okrug", 2003).

However, the considered solutions are not effective enough for the required studies, since the eggs of some trematodes, fasciol and paramphist, do not float, the solutions do not allow ticks, insects, their individual stages of development from scrapings from animals and birds, from substrates, from objects of the environment and from fecal matter. In addition, crystallization of salt in a liquid film on a glass slide makes it difficult to detect helminth eggs, and makes it impossible to detect protozoa oocysts.

The study of feces using Darling liquid is carried out as follows: the fecal sediment is mixed with the liquid and centrifuged for 2 minutes at a speed of 1500 rpm, which leads to flotation. Helminth eggs float onto a surface film in a test tube, from which they are removed by a coprological loop to detect oocysts of protozoa, helminth eggs from fecal matter.

Darling fluid allows for coprological studies, but the relative density of Darling fluid (1.20-1.22 g / ml) does not allow its use in the diagnosis of trematodoses.

The objective of the invention is the creation of a fluid with multifunctional capabilities for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, their individual stages of development from scrapings from animals, birds, from substrates, from objects of the environment and fecal masses, by their precipitation and flotation.

The technical result of the task is achieved by the fact that the fluid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, their individual stages of development includes the following components: the first is water - 48% and sodium chloride - 25%, second: also in the form of a liquid containing: sodium lauryl sulfate - 16.3%, (saturated salt solution), polyethylene glycol - 10%, sodium benzoate - 0.7%, while the first and second components were mixed together in a 1: 1 ratio . The fluid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of various classes, ticks, insects, their individual stages of development is homogeneous, opalescent, translucent, syrup-like consistency.

The finished liquid was poured into a flask, labeled and used as follows. First, by centrifugation of substrates, environmental objects, and fecal matter, a precipitate was obtained containing coccidium oocysts, cysts of balantidia and giardia (giardia), helminth eggs of various classes (nematodes, trematodes and cestodes), ticks, insects, their individual stages of development from scrapings from animals, birds mixed with the prepared liquid. The resulting mixture was again centrifuged for 4-5 min with a centrifuge rotation speed of 1500 rpm, which led to the flotation of the objects of study that were in the upper layer of the tube liquid. For microscopy, the objects of study were removed by a coprological loop from a surface liquid film.

Due to the difference in the relative density of the liquid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, and their individual stages of development (1.33-1.36 g / l) and objects of research, they floated and were in its upper layer. Isolation of coccidia oocysts, cysts of balantidia and giardia (giardia), helminth eggs of different classes (nematodes, trematodes and cestodes), ticks, insects, their individual stages of development from scrapings from animals, birds, from substrates, from environmental objects and fecal masses, by precipitation and flotation, they were carried out according to the generally accepted method using the created liquid. The effectiveness of research reached 100%.

Practical testing was carried out in animal husbandry and livestock farms of the Leningrad region. The clinic of the St. Petersburg State Academy of Veterinary Medicine examined animals: dogs, cats, fur-bearing animals, and cattle.

When examining animals that were previously diagnosed: sarcoptosis, otodectosis, chorioptosis, demodecosis, psoroptosis, heyletiellosis, bovicolez, in each case a traditional scraping was done, but it was placed in a test tube with a liquid for the diagnosis of coccidia oocysts, balantidia cysts, and helminths of different classes, ticks, insects, and their individual stages of development.

Then the mixture was centrifuged for 4-5 min at a rotation of 1500 rpm, then the upper film was removed with a coprological loop, placed on a glass slide and microscopic, and in 100% of cases, a preliminary diagnosis was confirmed by laboratory examination, establishing the final one. The substrates collected in livestock buildings and fur farms (dust, accumulation of dirt, grease, a mixture of litter and wool, etc.) were examined using an already developed method using the created fluid, and in 100% of cases all of the above parasites were found. Tables 1 and 2 show the results of testing.

Table 1 Diagnostic results of arachnoentomiasis using fluid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, and their individual stages of development. Group belly. (n) Disease Sarcoptosis,% lesion. Otodectosis,% lesion. Chorioptosis,% lesion. Demodecosis,% lesion. Psoroptosis,% lesion. Cheyletiosis,% lesion. Bovikolez,% lesion. Dogs (n = 150) 12 24 0 42 0 10 0 Cats (n = 210) 18.6 80 0 0 0 1.4 0 Arctic foxes (n = 320) 45 55 0 0 0 0 0 Cattle * (n = 180) 1,1 0 38.9 30.6 39 0 twenty Note: * - in cattle, a combined manifestation of chorioptosis, demodicosis and bovicolez was revealed.

table 2 The results of the detection of helminthiases and protozoa when using liquid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, and their individual stages of development. Group of animals (n) Disease Ascaridatoses,% lesion. Trichocephalosis,% lesion. Trematodoses,% lesion. Strongilatoses,% lesion. Protozoa,% lesion. Dogs (n = 150) 68.6 2.85 0 5.7 38.6 Cats (n = 210) thirty 0 four 0 fifty Arctic foxes (n = 320) 72 0 5 2 40 Cattle (n = 180) 1,2 10 fifty 24 twenty Note: In all the studied groups, combined invasion was detected in individual animals.

In contrast to the known fluids, the created fluid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, and their individual stages of development allows us to examine substrates (for example, dust) located in animal breeding and livestock buildings. For this purpose, weighed samples of 1 g samples of samples were taken, mixed with the created liquid and centrifuged for 4-5 min at 1500 rpm, then the top layer was removed with a coprological loop, placed on a glass slide and microscopy was performed.

Thus, the results of the above experiments allowed us to conclude: the use of the created fluid in diagnostic studies allowed 100% confirmation of the diagnoses in all cases by identifying coccidia oocysts, balantidia and giardia cysts (giardia), helminth eggs of different classes (nematodes, trematodes and cestodes), ticks, insects, their individual stages of development from scrapings from animals, birds, from substrates, from objects of the environment and fecal masses. In addition, compared with the above and other known liquids (Darling liquid), the created liquid is an original composition and has several advantages:

1. Express diagnosticum for laboratory research;

2. Allows you to use it in confirming diagnoses not only for helminthiases and intestinal protozoa, but also for sarcoptosis, demodectic and some entomoses, ie increases the range of detectable diseases;

3. The action of the components allows you to clean the scrapings and samples of fecal matter from related artifacts that make it difficult to view the preparations under microscopy;

4. The components that make up the liquid do not crystallize;

5. The liquid allows you to enhance the effect of the sharpness of the object in the microscope;

6. The composition of the liquid includes sodium benzonate, which prevents the development of bacterial microflora, so the prepared liquid can be stored for more than 30 days;

7. The liquid in the proposed composition is not toxic to humans;

8. The liquid does not require special storage and transportation conditions, in daylight it does not lose its properties, it remains homogeneous;

9. The liquid can be used extempore after its preparation.

Claims (1)

The fluid for the diagnosis of coccidia oocysts, cysts of balantidia and giardia, helminth eggs of different classes, ticks, insects, their individual stages of development contains the following components: the first is water - 48% and sodium chloride - 25%, the second also in the form of a liquid containing: sodium lauryl sulfate - 16.3%, saturated salt solution, polyethylene glycol - 10%, sodium benzoate - 0.7%, while the first and second components were mixed together in a 1: 1 ratio, while the liquid is homogeneous, opalescent, translucent, syrupy consistency.