RU2466713C1 - Method for producing biopreparation ferrigel - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: method for producing a biopreparation involves mixing of ferric oxyhydroxide with a water-soluble polymers and ultrasonic treatment of the suspension differing by the fact that there are used ferric oxihydroxide gel (FOH-gel) recovered at the stages of underwater deironing, the water-soluble polymer, and additionally glycerol is introduced in the following proportions, wt %: FOH-gel 50-60; water-soluble polymer 2.5-3; glycerol 10-15; water to 100. The water-soluble polymer is presented by a polymer specified in a group of polyvinyl alcohol (PVA), polyethylene glycol (PEG), polyvinylpyrrolidone (PVP) or polyacrylamide (PAA). The biopreparation possesses wound healing, bactericidal, regenerative and adsorption properties.

EFFECT: simplified method for producing the known biopreparation.

2 cl, 3 tbl, 4 ex

Description

The invention relates to chemical technology and medicine, in particular to the production of a biological product for the treatment of infected wounds, burns, skin diseases, diabetic foot and adsorption of microorganisms, as well as to the disposal of waste allocated to the deferrization station of underground waters extracted for household needs - iron oxyhydroxide ( OHL-gel).

The biological activity of metals has been known since the last century. The authors of the work (Materials of the 2nd All-Russian Conference on Nanomaterials, 2007, Novosibirsk, p. 323.) Studied the biological activity of iron, magnesium and copper nanoparticles and established the high biological activity of these metals and their oxide forms.

Known accelerating wound healing drug multifunctional prolonged action (Pat. 2306141, 2007). The disadvantages of this drug include multi-stage production. First, magnesium nanoparticles are obtained, then in glycerol they are subjected to ultrasound under special conditions. Next, separately dissolve nipogim in polyethylene glycol (PEG) and the resulting PEG solution is mixed with a glycerin suspension of magnesium nanoparticles, mixed and sent for homogenization on a roll maser.

The closest to the proposed method for producing a biological product is a wound healing agent and a method for its production (Pat. 2383349, 2008), the disadvantages of which include the fact that the precipitated precipitate of iron oxide-hydroxide (OGF) is used in dispersed form in the form of a dried powder obtained when drying the coolant extracted from groundwater. When dried, the OGL is aggregated and obtained in the form of large granules. To obtain a biological product it is necessary to grind and sift through a sieve - this is a very time-consuming stage, requiring special conditions and equipment.

The objective of the invention is to obtain a biologically active drug with wound healing, regenerating, bactericidal, adsorption properties, multifunctional, prolonged action, actively affecting the healing processes, regenerating processes in the skin, is well compatible with the biological environment of the body and stable during storage, and also is recycling iron-containing waste generated in a gel-like state at the deferrization station of groundwater extracted for ozyaystvennyh needs.

The technical result is to simplify the method of obtaining and improving the quality of the biological product and is achieved by the fact that the active component is a gel-like iron oxyhydroxide (OHL-gel), extracted from groundwater at deferrization stations, mixed and thoroughly mixed with an aqueous polymer solution and glycerin in a ratio of components, wt.%:

OHG gel 50-60 Water soluble polymer 2.5-3 Glycerol 10-15 Water up to 100.

The suspension is subjected to sonication for 30-60 minutes. Polyvinyl alcohol (PVA), polyethylene glycol (PEG), polyacrylamide (PAA), polyvinylpyrrolidone (PVP) are used as a water-soluble polymer.

The gel-like, creamy consistency of the sediment accumulated in the sump at the underground water deferrization stations is pre-filtered through a sieve with a pore size of 300 μm. After separation of the solid particles, the filtrate containing dispersed iron oxyhydroxide is separated into 2 layers upon settling: the upper, cloudy, merges, and the lower, creamy, is poured with clean water in a 1: 1 ratio, mixes thoroughly and settles. The upper transparent layer is merged, and the lower creamy one is used to obtain a biological product. Rinsing with water can be repeated until a transparent top layer is obtained upon settling.

Examples of specific performance.

Example 1. 2.5 g of PEG-1500 is dissolved in 30 ml of water. To the resulting solution was added 10 g of glycerol. The resulting solution was mixed with 57.5 g OHL gel, mixed thoroughly and sonicated for 20 minutes. The resulting "Ferrigel" is used to treat patients. The results of treatment of patients are shown in table 1, 2 and 3.

Example 2. 2.5 g of PVA is dissolved in 22.5 ml of water. To the resulting solution was added 15 g of glycerol. The resulting solution was mixed with 60 g OHL gel, mixed thoroughly and sonicated for 20 minutes. The resulting "Ferrigel" is used to treat patients. The results of treatment of patients are shown in table 1, 2 and 3.

Example 3. 3 g of PVP is dissolved in 37 ml of water. To the resulting solution was added 10 g of glycerol. The resulting solution was mixed with 50 g OHL gel, mixed thoroughly and sonicated for 20 minutes. The resulting "Ferrigel" is used to treat patients. The results of treatment of patients are shown in table 1, 2 and 3.

Example 4. 2 g of PAA is dissolved in 28 ml of water. To the resulting solution was added 10 g of glycerol. The resulting solution was mixed with 60 g OHL gel, mixed thoroughly and sonicated for 20 minutes.

The resulting "Ferrigel" is used to treat patients. The results of treatment of patients are shown in table 1, 2 and 3.

For comparison, Table 1 shows the results of treatment of patients with Levomikol used in practice (Registration number P No. 003107/01), which includes the antibiotic levomycetin.

Thus, the proposed method allows to obtain an effective biological product "Ferrigel" for the treatment of infected wounds, burns, skin diseases, diabetic foot and adsorption of microorganisms.

Table 1 The results of treatment of patients with “Ferrigel” and “Levomikol” Indicators Ferrigel treatment Treatment with Levomikol * Control group Duration of wound cleansing from the start of treatment, in days 3.5 ± 0.4 8.0 ± 0.5 11.0 ± 3.0 Duration of complete wound healing from the start of treatment, in days 14.5 ± 2.0 21.5 ± 3.0 26.5 ± 3.0

* "Levomikol" - a commercial preparation

table 2 Test results of the wound healing preparation "Ferrigel" based on OGZH-Tel No. p / p Composition and conditions of preparation Number of patients Allergy quantity one OHG gel, initial, vibration 10 thirty 2 OGZH-gel-UZ 10 10 3 OHL-gel + (PEG-15) 2.5% + Glycerin 10% 10 one four OHL-gel + PVA-2.5% + Glycerin-15% 12 no 5 OHL-gel + PVA, 1% + Glycerin-10% 10 no 6 OHL-gel + PVP, 3% + Glycerin-15% 10 no 7 OHL-gel + PAA, 3% + Glycerin-15% 10 no 8 OHL-gel + PVA-3.0% + Glycerin 15% 10 no 9 OHL-gel + PEG-2.5% + Glycerin -15% 12 no All preparations, except the first, were treated with ultrasound, No. 2-8 - for 30 minutes, No. 9 -60 minutes.

Table 3 The results of the treatment of skin diseases with the preparation "Ferrigel" based on OGF-gel * Pathology Number of patients Exudation, days Proliferation days Complications (allergy) Diabetic foot 8 12 eighteen 2 Infected wounds four 3 5 no Burns 2 5 7 no Boils, abscesses, carbuncles four four 6 no * Composition of the preparation: OHL-gel + PVA-3% + glycerin-15%, treated at t = 100 ° C with ultrasound (60 min)

Claims (2)

1. A method of obtaining a biological product, comprising mixing iron oxyhydroxide with a water-soluble polymer, followed by sonication of the suspension, characterized in that they use a gel-like iron oxyhydroxide (OHL-gel), isolated at underground water deferrization stations, a water-soluble polymer and additionally introduce glycerin in the ratio of components, wt.%:
OHG gel 50-60 Water soluble polymer 2.5-3 Glycerol 10-15 Water Up to 100. 2. The method according to claim 1, characterized in that as a water-soluble polymer using a polymer selected from the group of polyvinyl alcohol (PVA), polyethylene glycol (PEG), polyvinylpyrrolidone (PVP) or polyacrylamide (PAA).