RU2456674C1 - Method for simulating atrophic skin scar - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: atrophic skin scar is simulated by introducing an injured preparation into deep layers of reticular dermis close to a border with hypodermis. As a preparation, Dermastabilon 0.1 ml is used. The preparation is introduced in a rat at the distance of 2 cm from a spinal column along the length of 1 mm of a needle of an insulin syringe at an angle of 45° to form a papula.

EFFECT: method provides easy-to-reproduce simulation of the atrophic skin scar with a dystrophic behaviour characteristics during formation approximated to the actual clinical pathology.

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Description

The invention relates to medicine, namely to dermatology, and can be used to study the processes of formation of atrophic scars, their correction and dystrophic processes in the dermis.

The prototype of the present invention is a method for modeling atrophic scars, which consists in the fact that in the thickness of the mesh layer for the entire length of the needle of an insulin syringe parallel to the skin surface with smooth pulling of the needle and uniform pressure on the syringe plunger, 0.2 ml of collagenase solution with a content of at least 100 KE (patent RU 2384892, 2010). The disadvantages of this technique are the systemic effects of the drug administered, which is atypical for acne, which is the most common cause of atrophic scars, the depth of the simulated lysis of the connective tissue base of the skin - in case of acne, inflammatory processes accompanied by enzymatic destruction of the dermis occur in the sebaceous glands located in the deep layers of the dermis and at the border of the dermis and subcutaneous fat, while, according to the above method, the drug that destroys the connective tissue, is introduced into the middle layers of the dermis, and the method of administration is linear, while with acne and other inflammatory diseases that result in the formation of atrophic scars, the rounded lesions are more characteristic, which can significantly alienate the simulated pathological process from its clinical course in patients with acne.

The objective of the invention is the development of near-pathogenesis of the formation of atrophic scars in the clinic, technically simple to implement, easily reproducible method for modeling atrophic scars of the skin, expanding the arsenal of tools to create a model of atrophic scar.

The technical result when using the invention is the formation of an atrophic scar of the skin, close in characteristics of its course to real clinical pathology.

The specified technical result is achieved by the fact that in the method for modeling atrophic scars, including a single injection of the drug into the dermis, according to the invention, 0.1 ml of the dermastabilone solution is administered as the drug, while the drug is administered to the rat at a distance of 2 cm from the spine for a length of 1 mm of an insulin needle syringe at an angle of 45 ° with the formation of a papule.

The proposed method for modeling atrophic skin scar is as follows. As experimental animals use white rats. Produce mechanical depilation of the skin of the back of the animal. Then, within one hour, exposure to level local irritation, which may be caused by mechanical action during depilation. Under local application anesthesia (Emla cream, holding for 15 minutes under an occlusal film), at a distance of 2 cm from the spine, into the deep layers of the reticular dermis, near the border with hypodermis, a 1 mm insulin syringe needle is inserted at a 45 ° angle, which approximately corresponds to the depth of administration to the thickness of the rat dermis - 0.6-0.7 mm, as a result of which a papule forms on the surface of the skin. The needle length is 1.6 cm, the diameter is 0.40G. To comply with the conditions for introducing the needle to the required depth, visual inspection is performed - the tip of the needle does not need to be clearly contoured when it is raised, while only the skin roller is visually determined. Then, with a smooth and uniform pressure, 0.1 ml of a solution containing 50 mg / ml phosphatidylcholine and 20 mg / ml deoxycholate (bile salt) is injected onto the syringe plunger (Dermastabilon No. FS-2006 / 057-04 dated 04/27/2006.) . Under the influence of deoxycholate and phosphatidylcholine, lysis of the cellular elements of the skin and subcutaneous fat, emulsification of the contents of adipocytes is realized, which simulates the formation of negative tissue in case of acne. As a result, a linear atrophic cicatricial defect is formed in the area of projection onto the skin surface of the intradermally injected volume of Dermastabilon. A pronounced atrophic defect is formed within 7-10 days.

To assess the reliability of the results of the proposed method for modeling an atrophic skin scar, dermatoscopy was performed with photo documentation. The technique was tested on 15 rats. Animals were kept in a vivarium in accordance with the “Sanitary Rules for the Design, Equipment and Maintenance of Experimental Biological Facilities (Vivariums)” approved by the Ministry of Health of the USSR on 07/06/1973 and order of the Ministry of Health of the USSR No. 163 of 03/10/1966. Work on rats was carried out in in accordance with the principles of humane treatment of experimental animals, approved by order of the Ministry of Health of the USSR No. 755 of 08/12/1977 and No. 701 of 07/27/1978.

The proposed method is illustrated by the following figures.

Figure 1 shows the appearance of deep dermal papulla at the injection site, immediately after administration.

Figure 2 - maximum edema and hemorrhage at the injection site after 3 hours.

Figure 3 - scar formation with hemorrhagic crust above it on the 3rd day of the experiment.

Figure 4 - formed atrophic scar after discharge of the crust on the 8th day.

The proposed method allows to determine the stage of formation of atrophic skin scars, which allows experiments to treat them at different stages of formation.

The proposed method is illustrated by the following example: a rat, a male of the Wistar strain, weighing 250-300 g, under local application anesthesia at a distance of 2 cm from the spine, into the deep layers of the reticular dermis, close to the border with hypodermis, papularly injected to a length of 1 mm a needle of an insulin syringe at an angle of 45 °, the drug "Dermastabilon". On the 8th day, an atrophic scar formed on the skin surface above the injection site.

Claims (1)

A method for modeling atrophic scars, including a single injection of the drug into the dermis, characterized in that 0.1 ml of the Dermastabilon solution is administered as the drug, while the drug is administered to the rat at a distance of 2 cm from the spine to a length of 1 mm of an insulin syringe needle at an angle of 45 ° C papule formation.

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