RU2420323C2 - Resuscitation procedure in zootoxin poisoning - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine, and can be used in zootoxin poisoning. That is ensured by thermal action of external temperature 50°C on the whole body for 30-45 minutes.

EFFECT: procedure enables higher human tolerance to zootoxins of various effects due to the adjustment of optimum temperature and body exposure time to provide the most effective detoxification.

2 tbl, 1 ex

Description

The invention relates to the field of experimental medicine and veterinary medicine.

A widely known method of treatment for acute poisoning by snake venoms with novocaine blockade (see, for example, Biletsky D.F. Use of a novocaine block for snake bites // Medical practice, 1954, No. 8, p. 755-756; Osipovsky V.M. Novokainovy Vishnevsky block as a method of treating snake venom poisoning // Kazan Medical Journal, 1987, No. 5, p. 604-610, etc.).

The disadvantage of this method is the relatively low treatment efficiency.

It is also known the use of other means to prevent the development of neurotoxic, hemorrhagic, blood-clotting and edematous-necrotic effects of snake venom in case of poisoning the body.

So, according to the patent of the Russian Federation No. 2063223, 07/10/1996, as a means of correcting indicators of the immune status of people who have been bitten by snakes, karakurt, scorpion, etc., a solution of “STEAROKS-920” or “OS-20” is administered subcutaneously. According to RF patent No. 2228194, 05/10/2004, octreotide is introduced into the body at a dose of 50 μg / 100 g body weight.

It is also known to use a complex of drugs in combination with anti-snake serum in experimental poisoning with cobra venom (see. The mechanism of action of zootoxins. Gorky, 1980, p.135-143).

A common disadvantage of the above funds is the relatively low effectiveness of the treatment. The monospecific nature of the antidote makes it ineffective when used for an unidentified zootoxin, since the type of stinging snake or insect cannot always be known. In addition, high ambient temperatures cause loss of serum activity.

To eliminate this drawback, the method according to the patent of the Russian Federation No. 21114636, 07/10/1998, according to which a mixture of at least two antisera developed in relation to various poisons is used as an antidote to the bite of a poisonous animal, is directed.

However, even in the case of “mixed monospecific antidote” described in patent No. 2141463, it is not possible to efficiently neutralize all possible zootoxins. In reality, it is necessary to take into account a limited set of zootoxins characteristic of a given geographical area, taking into account the relative frequency of bites of people by specific species or subspecies of a poisonous animal, against the poison of which an antiserum is produced.

There is also a method of resuscitation in case of poisoning with neurotoxic poisons according to RF patent No. 2191609, 10.27.2002, which consists in exposing a patient to a venous or arterial blood perfused through a sorbent column with a pulsed electromagnetic field of a certain frequency and intensity.

This method is the closest to the claimed method of resuscitation in technical essence, because it uses the impact on the body of a physical factor - a pulsed electromagnetic field.

Due to the extracorporeal effect of pulsed magnetic fields on the blood flowing in the hemosorption system, the basic hemodynamic parameters are significantly improved.

The disadvantages of the prototype method include the limited scope of its application, due to the need to use special equipment for magnetic hemotherapy, not available in every clinic (not to mention the "field" conditions), as well as its intended use for the treatment of poisoning by poisons of predominantly neurotoxic effects.

The technical result of the invention is the expansion of the scope and simplification of the method of resuscitation in case of poisoning with zootoxins of various origins.

The specified result is achieved by the fact that in the resuscitation method for poisoning with zotoxins, which consists in exposing the body to a physical factor, according to the invention, a high external temperature is used as a physical factor affecting the body.

The invention characterized by the above essential features at the filing date of the application is not known in the Russian Federation and abroad and meets the requirements of the criterion of "novelty."

The invention can be implemented industrially using known technical means and technologies, which allows us to conclude that it meets the requirements of the criterion of "industrial applicability".

At the filing date, the effect of high external temperature on increasing the body's resistance to the effects of zootoxins is not known, and therefore it can be concluded that the patentability condition is “inventive step”.

An example of a specific implementation of the method

The studies were performed on 150 laboratory non-linear male rats weighing 200 ± 10 g. The introduction of zootoxins was carried out intraperitoneally 10 minutes before heating at the doses indicated in tables 1 and 2. Control animals received physiological saline in the same volume. The conditions of an overheating microclimate (50 ° С, 30% humidity) were created in a climatic chamber.

Table 1 The number of surviving white rats (%) after thermal exposure (T = 50 ° C) for 45 min under the action of zootoxins Investigated Zootoxins The number of surviving individuals (%) at 20 ° C Time after thermal exposure (h) 1,0 6.0 24.0 Fiziol. rr (control) one hundred 0.00 0.00 0.00 Cobra venom (1 mg / kg) 0.00 90 80 60 Efa venom (6 mg / kg) 0.00 one hundred one hundred one hundred Thyroid Mud Poison (4 mg / kg) 0.00 one hundred one hundred one hundred Gyurza venom (4 mg / kg) 0.00 80 70 fifty Viper Venom (1 mg / kg) 10 70 60 fifty Bee venom (2 mg / kg) twenty one hundred one hundred one hundred Scorpion venom (1 mg / kg) 0.00 one hundred 80 70 Toad venom (2 mg / kg) twenty 90 80 80

The introduction of zootoxins under normothermic conditions (20 ° C) was accompanied in most cases by 100% death of experimental animals, and only with the introduction of vipers, bees and toads, the number of surviving individuals ranged from 10 to 20% (Tables 1 and 2).

With the combined effect of zootoxins and a high external temperature (50 ° C), from 50 to 100% of individuals survived a day after exposure (Table 1).

A similar picture was observed under the same experimental conditions, characterized by a decrease in the thermal exposure time to 30 min. In this case, the number of surviving individuals during the day was significantly higher (Table 2).

table 2 The number of surviving white rats (%) after thermal exposure (T = 50 ° C) for 30 min under the action of zootoxins Investigated Zootoxins The number of surviving individuals (%) at 20 ° C Time after thermal exposure (h) 1,0 6.0 24.0 Fiziol. rr (control) one hundred 40 0.00 0.00 Cobra venom (1 mg / kg) 0.00 one hundred one hundred 80 Efa venom (6 mg / kg) 0.00 one hundred one hundred one hundred Thyroid Mud Poison (4 mg / kg) 0.00 one hundred one hundred one hundred Gyurza venom (4 mg / kg) 0.00 one hundred 90 70 Viper Venom (1 mg / kg) 10 one hundred 80 70 Bee venom (2 mg / kg) twenty one hundred 90 90 Scorpion venom (1 mg / kg) 0.00 one hundred 90 80 Toad venom (2 mg / kg) twenty one hundred one hundred 90

The obtained experimental data indicate the high efficiency of the proposed method of resuscitation. Exposure to high external temperatures significantly increases the body's survival when exposed to a wide range of zootoxins.

Claims (1)

The method of resuscitation in case of poisoning with zootoxins, which consists in thermal effects on the body, characterized in that the thermal effect is carried out on the whole body at an external temperature of 50 ° C for 30-45 minutes