RU2413941C1 - Method of determining degree of risk of malignant growth - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: blood serum is sampled, thermostated at temperature 37°C for 20-30 minutes, after that a drop of serum is placed under cover glass, dried during 7-10 days and microscoped under cover glass at room temperature and 55-60% humidity. If small spherulites, located outside the borders of large anisotropic spherulites, on the borders of large anisotropic spherulites or within the borders of large spherulites, are detected. Low, moderate or high degree of risk of malignant growth is determined respectively.

EFFECT: method application makes it possible to increase possibility of determining degree of risk of malignant growth.

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Description

The invention relates to medicine, namely to laboratory research.

The need to search for risk markers for malignant growth is due to the fact that identifying the likelihood of malignant growth allows timely intervention to exclude the occurrence of the disease.

A known method for the diagnosis of cancer or the likelihood of its occurrence, in which they study a weak aqueous solution of native plasma or native blood serum of a patient by laser correlation spectroscopy (RF Patent No. 2219549, IPC G01N 33/49, publ. 2003).

The disadvantage of this method is the technological complexity of the method: the need to use expensive equipment, chemicals, and the obtained characteristic parameters do not have a high degree of reliability of the conclusion. The closest is a method for the diagnosis of malignant neoplasms (RF Patent No. 2235323, IPC G01N 33/48, publ. 2004), which includes obtaining blood serum, drying the drop taken from it under a coverslip, microscopy in polarized light at room temperature and humidity 55-60 %, detection in the morphological picture of the dried drop of large anisotropic spherulites.

The disadvantage of this method is that the diagnosed oncological process at the preclinical stage of development cannot have spontaneous reverse development and requires the intervention of oncologists.

The task of the invention is to remedy these shortcomings, prevent the preclinical stage of the development of cancer, which will provide an opportunity to eliminate the risk of malignant growth, including non-pharmacological methods.

To this end, in a method for determining the degree of risk of malignant growth, including obtaining blood serum, drying a drop taken from it under a coverslip, microscopy in polarized light at room temperature and humidity of 55-60%, revealing large anisotropic spherulites in the morphological picture, a selected blood serum is proposed thermostat at a temperature of 37 ° C for 20-30 minutes. Then, a drop of blood serum is dried for 7-10 days, the presence of small spherulites and their localization with respect to large ones are determined, and if small spherulites located outside the boundaries of large anisotropic spherulites are detected, a weak one is identified at the border of large anisotropic spherulites or within the boundaries of large spherulites , moderate or high risk of malignant growth, respectively.

Preliminary thermostating of blood serum at 37 ° C and prolonged dehydration for 7-10 days contributes to the structural restructuring of its components: alien molecular particles identical in physicochemical properties (possibly antibodies in combination with antigens) enter the same rhythm of oscillations in patients with risk of malignant growth are embedded in physiological structures (protein-lipid structures for building membranes, cell nuclei), which is manifested in the morphological picture of blood serum, a specific locale atsiey large (physiological) and small (alien) spherulites.

Figure 1 - morphological picture during microscopy in polarized light of small spherulite located outside the boundaries of a large anisotropic spherulite; figure 2 - morphological picture of a large anisotropic spherulite; figure 3 - small spherulite at the border of a large anisotropic spherulite; in Fig 4 - small spherulite inside the boundaries of large spherulite; figure 5 - the presence of defects and residues of fragments of small spherulites on large spherulite.

The method is as follows.

Blood is taken from a patient from a finger or from a vein, blood serum is obtained by sedimentation or centrifugation, then a test tube with blood serum is placed in a thermostat for 20-30 minutes at a temperature of 37 ° C, after which it is applied to the surface of a glass slide 0.01-0, 02 ml, covered with a coverslip (creating an analytical cell) and left in a working room at a temperature of 20-25 ° C and a relative humidity of 55-60%. After 7-10 days of slow dehydration, the analytical cell is microscopied in polarized light and, in the presence of spherulites of different sizes, the localization of small relatively large spherulites is determined.

Example 1

Patient A. Clinical examination. The result of the study of the morphological picture of blood serum in this way revealed the presence of large and small spherulites, while some small spherulites are localized at the border of large ones.

Conclusion: a low degree of risk of malignant growth (figure 1). It is recommended to change the lifestyle, sanitize the foci of pathological processes.

After a month, when re-examining the morphological picture of the blood serum of patient A., only large spherulites were determined (Fig. 2).

Example 2

Patient K. Clinical examination. The result of the study of the morphological picture of blood serum in this way revealed the presence of large and small spherulites, while some small spherulites are localized in the large marginal zone.

Conclusion: moderate risk of malignant growth (figure 3). Recommended examination, lifestyle changes, rehabilitation of foci of pathological processes.

After a month, when re-examining the morphological pattern of blood serum in patient K., only large spherulites were determined (Fig. 2).

Example 3

Patient I. Clinical examination. The result of the study of the morphological picture of blood serum in this way revealed the presence of large and small spherulites, while some small spherulites were localized inside large ones.

Conclusion: a high risk of malignant growth (figure 4). A full examination is recommended, lifestyle changes, sanitation of foci of pathological processes.

A month later, when re-examining the morphological picture of the blood serum of patient I., large spherulites were determined (Fig. 2), some with defects (Fig. 5); three months later, when re-examining the morphological pattern of blood serum in patient I., only large spherulites were determined (Fig. 2).

The proposed method allows to identify a different degree of risk of malignant growth, to give recommendations for further examination and lifestyle changes with subsequent monitoring of the effectiveness of preventive measures.

Claims (1)

A method for determining the degree of risk of malignant growth, including obtaining blood serum, drying the drop taken from it under a coverslip, microscopy in polarized light at room temperature and humidity 55-60%, revealing large anisotropic spherulites in the morphological picture, characterized in that the selected blood serum thermostat at a temperature of 37 ° C for 20-30 minutes, then a drop of blood serum is dried for 7-10 days, determine the presence of small spherulites and their localization in relation to ajor and the detection of small spherulites located outside the boundaries of large anisotropic spherulites, at the border of large anisotropic spherulite boundaries or inside large spherulites define weak, moderate, or high risk of malignant growth, respectively.

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