RU2407278C1 - Method to detect growth power of winter crop - Google Patents

Abstract

FIELD: agriculture. ^ SUBSTANCE: invention is related to agriculture, namely, to selection of winter crops. Method includes making cuts of plant sprouts, placement of obtained cuts onto slide plate, soaking of cuts with solution of acid fuchsine with subsequent curing for 10-15 minutes and visual inspection of preparations under microscope. Sprouts are cut across its growth cone, then they are placed onto dry slide plate, and cuts are only soaked at their sides. ^ EFFECT: method reduces labour intensiveness and increases validity of plant sprout growth power assessment.

Description

The invention relates to agriculture, namely to the selection of winter crops.

A known method for determining the degree of viability of a shoot of a winter crop plant, including making shoot sections of a plant along its growth cone, placing the slices on a glass slide and wetting them with a solution of acid fuchsin, previously applied as a drop on a glass slide, after holding it for a quarter hour, washing and coating with a cover glass, the sections are examined under a microscope at a magnification of 70-100 times [1].

The disadvantages of this method is the relative complexity of the method due to the necessity of washing sections of the plant, and washing reduces the clarity of the distinction between living and damaged tissues, which reduces the accuracy of the analysis.

The objective of the invention is to reduce the complexity and increase the reliability of the method.

The proposed method, as well as the known one, includes performing shoot slices, placing the obtained slices on a glass slide, wetting the slices with an aqueous solution of fuchsin, followed by exposure for 10-15 minutes and examining the preparations under a microscope. In contrast to the known method, in the proposed method, the shoot sections are performed across its growth cone, while the sections are placed on a dry glass slide, and the sections are wetted only on their sides.

The method is as follows.

On the shoots, the vaginal part of the dead leaves is removed and the upper part of the shoot is cut with a razor at a height of 5 mm from its base. In overgrown plants, the growth cone is slightly extended, therefore, it is necessary to first expose the growth cone and only after that make transverse sections so as to capture the growth cone and 2 mm of its base. From each tillering shoot 10-12 pieces of slices 0.3-0.5 mm thick are made. As a rule, the slices from each plant are located on one slide, where each shoot has its own series of slices, where they are stained with a solution of acid fuchsin, obtained in the consistency of 0.5 g of acid fuchsin per 10 ml of 96% ethyl alcohol. With such a consistency of the resulting solution for a time sufficient for visual analysis of the sections, ethyl alcohol does not change the state of living cells, but increases the permeability of the dye in frost-damaged cells. The boundary of cell damage in slices becomes clearer. The dye is applied to the sections with a pipette, and the sides of the sections are wetted without wetting their surface to be cut, and after 10-15 minutes, the preparations are examined under a microscope. Pre-rinsing with water of stained sections is not performed.

On the transverse sections, the tillering shoots state, not yet isolated from the main one, is clearly visible. When viewing transverse sections, conductive bundles of living tissues are visible as intensely colored dots surrounded by unpainted cells, which provides reliable distinguishability of living and damaged tissues.

In the proposed method, analysis is simplified and accelerated by eliminating the washing of plant sections, which also prevents the dye from being washed out during washing and provides a clearer distinction between living and damaged tissues.

Cross sections provide their qualitative analysis for production on particularly underdeveloped shoots, since one or two sections will necessarily pass through the growth cone.

The source of information

1. P.A. Vlasyuk, D.F. Protsenko, M.A. Gurileva. Winter hardiness of winter wheat in Ukraine. Kiev, 1959, p. 32, 41, 42.

Claims (1)

A method for determining the viability of a winter culture, including performing shoot sections of a plant, placing the slices on a glass slide, wetting the slices with a solution of acid fuchsin, followed by exposure for 10-15 minutes and examining the preparations under a microscope, characterized in that the shoot slices are performed across its growth cone , while the slices are placed on a dry glass slide, and the wetting of the slices is carried out only on their sides.