RU2396089C1 - Method of treating acute lactational mastitis in cows - Google Patents

Abstract

FIELD: medicine. ^ SUBSTANCE: invention refers to veterinary science. The method involves coating of empty udder skin with a thin layer of an ointment. The ointment contains Dimexid in amount - 1%, Vaseline - 85-95%, staphylococcal anatoxin - the rest. ^ EFFECT: use of the method improves clinical effectiveness, accelerates the recovery time. ^ 2 tbl

Description

There is a method of treatment and prevention of acute lactational mastitis in women using leukocyte suspensions for the treatment of patients with purulent lactational mastitis [Abstract of the thesis - Yu.A. Spesivtseva, 1995]. The essence of the method consists in the transfusion (transfusion) of leukemia from donors immunized with staphylococcal toxoid. Transfusion was carried out twice with an interval of one week in a volume of from 100 to 600 ml. Yu.A. Spesivtsev concluded that transfusions of leucites from immunized donors containing stimulated phagocytic cells have a deliberately mediated immunocorrective effect on the cellular immunity system of patients with purulent lactational mastitis and are a highly effective means of preventing generalization of infection.

The disadvantages of the considered method are as follows:

donors and the receipt of a large volume of leukocyte suspension are necessary; intravenous administration of the drug, which requires fixing the patient and a significant investment of time specialists. In addition, this method is expensive, requiring a high level of qualification of specialists, the availability of expensive equipment and reagents, and therefore it is almost not suitable for the treatment of acute lactation mastitis in cows in industrial production.

Known for the closest in technical essence and problem being solved is the method of treating acute lactation mastitis in cows with staphylococcal and streptococcal toxoids, which consists of introducing them intracisternally at a dose of 2.0 ml into the affected portion (developed by the Scientific Research Institute of the Agro-Industrial Complex, Kursk, 2003 - prototype).

The disadvantage of this method is the possibility of trauma to the channel of the milk tank, a specialist is required to take an analysis, prepare toxoids and administer the resulting preparations. Thus, this method is expensive, time-consuming and requires highly qualified specialists.

The objective of the invention is to increase the effectiveness of the treatment of acute lactational mastitis in cows in industrial production, through the use of drugs with directed anti-staphylococcal effect.

The technical result of the task is achieved by the fact that in the method of treating acute lactation mastitis of cows, it is proposed to apply an ointment to the skin of an emptied udder with a thin layer containing, for example, dimexide in an amount of 1%, petroleum jelly 85-95%, the rest is staphylococcal toxoid.

A method of treating acute lactational mastitis in cows is as follows.

Before performing therapeutic manipulations, the udder of the cow should be thoroughly washed and emptied. The ointment is applied in a thin layer on the affected udder lobes (from 1 to 4). The hands of the milkmaid applying the ointment should be protected with latex gloves, since the active substance of the ointment passes through the skin's epithelial barrier. The participation of a veterinarian is not required. The composition of the ointment includes:

1. The staphylococcal toxoid obtained by the factory method [instructions for use of TSA (antistaphylococcal preparation), registration number PVR 1.03.0154 - 96]. ASP provides stimulation of a specific immune response to various exotoxins and somatic antigens of staphylococci and provides rapid death of the pathogen in the body.

2. Dimexide (full name - dimethyl sulfoxide) has the ability to penetrate biological membranes, including through skin barriers and mucous membranes without damaging them. It shows a pronounced local analgesic activity in pain syndromes of various etiologies, and has anti-inflammatory and antimicrobial effects. Dimexide acts as a conductor inside the cell. We made an ointment based on TSA with the addition of dimexide. Vaseline is used as the base, but lanolin, which is expensive, can also be used.

The ointment is prepared as follows: in a sterile glass vessel with a capacity of 0.5 liter, the components are laid in the sequence: toxoid is added to the toxin, mixed thoroughly with a wooden spatula. Dimexide is poured into the resulting homogenized mixture slowly, with constant stirring, mixed thoroughly.

Options for the composition of the ointment.

1 option:

- petroleum jelly 95% (400 ml)

- toxoid 4% (16 ml)

- dimexide 1% (4 ml)

Option 2:

- petroleum jelly 85% (400 ml)

- toxoid 14% (56 ml)

- dimexide 1% (4 ml)

Different ratios of the components of the composition of the ointment are offered depending on the causative agent of the inflammatory process. In case of mastitis caused by S.aureus, we recommend using an ointment with a 14% content of toxoid; for other types of staphylococci, we recommend using an ointment with a 4% content of toxoid. The ointment is hermetically sealed and ready for use. It is stored in a cool place. The composition of the ointment with a 4% toxoid content is significantly cheaper in cost. Thus, there is a choice due to economic considerations.

The proposed composition of the ointment has the properties of activating a specific immune response, conducting the active substance of the TSA through intact skin, has analgesic, anti-inflammatory and antimicrobial effects.

The studies were carried out on cows kept in the conditions of the Leningrad region, in production conditions on lactating dairy cows of black-motley breed. To study the effect of the ointment, 3 groups of cows having various forms of acute mastitis were formed. Groups were selected taking into account analogues of one breed, age, with one degree of disease. For the treatment of patients with mastitis of cows of the 1st group, Mastisan ointment (containing prednisolone and a broad-spectrum antibiotic) was used. For the treatment of patients with mastitis of cows of the experimental group, the proposed ASP ointment was used. The control group was healthy cows.

The effectiveness of treatment was judged by the disappearance of clinical signs of inflammation of the udder and the negative results of rapid studies on mastitis. Examples of studies of the effectiveness of ointments based on TSA are given in tables No. 1, 2.

An analysis of the data revealed a decrease in the concentration of gamma globulins, BASK in all animals with mastitis. Studies have not revealed changes in serum concentrations of albumin, α, β - globulins, total protein, as well as the activity of lysozyme and circulating active complexes (CEC). Since the level of lysozyme is normal, a change in immunological parameters is not associated with antibacterial activity. The monocytic macrophage system is not disturbed, as indicated by the same level of CEC in all groups. A significant decrease in IgA and IgM was revealed, which is especially noticeable with this pathology, since IgA is the main immunoglobulin transported secretly through mammary gland epithelial cells, IgM is the first immunoglobulin to capture and remove trapped bacteria. It can be assumed that, based on an estimate of the concentration of IgA, it is possible to divide the livestock into those with a risk of developing mastitis (low IgA serum) and those without it (high IgA serum). According to tables No. 1 and 2, it can be seen that in case of cows with acute mastitis, antibodies of all three classes decrease. After treatment with Mastisan, the level of all three classes remains low.

After treatment of cow mastitis with an ASP-based ointment, the level of antibodies of the IgA, IgG class (IgG1 + IgG2 = IgG - immunoglobulins that determine the specificity of the immune response) returns to its original position. IgM level rises towards the normal value.

Table 1 The amount of immunoglobulins in serum, g / l The control Group 1 (the drug "Mastisan") Group 2 (The drug "ASP") For 1 day On day 14 For 1 day On day 14 IgA 4.6 ± 0.3 3.4 ± 0.2 3.5 ± 0.2 3.4 ± 0.2 4.7 ± 0.4 IgM 1.2 ± 0.3 0.5 ± 0.4 0.5 ± 0.07 0.5 ± 0.4 0.8 ± 0.2 IgG1 50.6 ± 0.4 47 ± 0.4 43.2 ± 0.7 47 ± 0.4 49.6 ± 0.7 IgG2 28.7 ± 0.9 18.6 ± 0.2 18.7 ± 0.1 18.6 ± 0.2 22.3 ± 0.3

table 2 Biochemical parameters of blood serum, g / l The control Group 1 (the drug "Mastisan") Group 2 (The drug "ASP") Before After For 1 day On day 14 treatment treatment Albumin 30.79 ± 4.1 34.6 ± 1.5 33.3 ± 2.4 34.6 ± 1.5 33.9 ± 5.1 α-globulins 19.8 ± 6.7 19.5 ± 2.8 17.3 ± 2.3 19.5 ± 2.8 15.2 ± 2.3 β-globulins 15.2 ± 2.3 14.6 ± 2.2 17.0 ± 2.2 14.6 ± 2.2 13.6 ± 1.9 gamma globulins 41.2 ± 3.8 31.1 ± 1.9 34.4 ± 3 31.1 ± 1.9 33.8 ± 4.9 CEC, opt. 0.2 ± 0.1 0.17 ± 0.01 0.15 ± 0.03 Lysozyme activity,% lysis 12.1 ± 0.4 12.1 ± 0.3 12.3 ± 0.3 12.1 ± 0.3 12.4 ± 0.4 BASK /% lysis of E.colli 59.8 ± 0.9 54.8 ± 1.7 56.7 ± 1.5 54.8 ± 1.7 55.6 ± 1.5 Total protein 74.6 ± 4.2 76.7 ± 2.1 79.1 ± 1.9 76.7 ± 2.1 74.9 ± 1.9

Claims (1)

A method of treating acute lactational mastitis in cows, which consists in applying an ointment containing 1% dimexide, vaseline - 85-95%, staphylococcal toxoid - the rest to the skin of an empty udder.