RU2377562C1 - Method of water analysis for biological activity - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to medicine, and can be used to analyse water for its biological activity that is ensured by preparing standard saline of water. Dead-end nerve cell preparations are prepared and placed in saline for 40 minutes. It is exposed to electric stimulation with four after-threshold intensity pulse packets at frequency 100 Hz. Packet length is 5 seconds with the interval 5 seconds. It is followed with discharging and recording excitatory postsynaptic potential amplitude of N-methyl-D-aspartate-receptor both initial at the moment of stimulation and 5, 15, 30 and 40 minutes after stimulation. Biological activity of water is determined by comparing initial parametres of caused cell activity during incubation in water and thereupon considering duration of staying in water.

EFFECT: method allows analysing water of various sources for biological activity either prior to and after processing and comparing levels thereof.

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Description

The invention relates to studies of the properties of water. Currently, it is accepted that water is a biologically neutral substance.

No analogues of the invention were found during the search.

The task is to determine at the molecular-cellular level the effect of water as a biologically active substance on the learning and memory of surviving nerve cells.

The essence of the proposed method for determining the biological activity of water consists in preparing a standard saline solution from water, preparing nerve cell surviving preparations, placing the cells in saline solution for a predetermined time, 40 min, electrical stimulation of the cells with 4 bursts of suprathreshold intensity pulses with a burst duration of 5 s, interval 5 s, with a frequency of f = 100 Hz, abduction and registration of the initial and after 5, 15, 30 and 40 minutes during incubation in water of potentials from the NMDA receptor - (N-methyl-D-aspartate) - total response activir yuschy potential compact group of neurons, and identifying the biologically active effects of water by comparing the initial parameters of the bioelectric activity caused by the cells during exposure to water, and after its completion at predetermined intervals on learning and memory nerve cells, depending on the length of time in water.

The proposed method is as follows. Prepare standard saline from test water (by distillation and dissolution of salts). Preparing surviving preparations of nerve cells in the form of sections of the nerve tissue of the brain, either single prepared, or in tissue culture. These nerve cells for a predetermined time, 40 min, are placed in saline and stimulate cells with an electric signal from 4 packets of pulses of suprathreshold intensity with a burst duration of 5 s, an interval of 5 s, with a frequency of 100 Hz. The initial bioelectrical activity of the cells is withdrawn and recorded during the incubation in water and upon its completion after 5, 15, 30 and 40 minutes at specified intervals for the convenience of comparing measurement results. The amplitude of the NMDA of the EPSP (Excitatory postsynaptic potential) is recorded. We used the model of long-term (minutes) post-stimulus potentiation, which characterizes the development of a "long-lasting" excess of the increase relative to the initial excitability in the nervous tissue after its "short-term" (second) high-frequency electrical stimulation. Reflects the development of learning and the formation of a trace of memory and learning in neurons.

Example 1

Investigated local tap water. The measurement results are shown in table 1.

Table 1 Recorded evoked potential of NMDA EPSP neuron receptor The amplitude of the evoked potential NMDA EPSP (μV) The initial amplitude at the time of stimulation 5 min after stimulation 15 min after stimulation 30 min after stimulation 40 min after stimulation NMDA UPU 68 125 148 143 137

As can be seen from the data presented, in the process of incubation of nerve cells in saline on the studied tap water, an increase in potential occurs. There is a stable excess of the initial amplitude level in all measurements. That is, there is training and the formation of memory in nerve cells under the influence of saline water.

Example 2

The biological activity of the same tap water purified in the "Water Treatment Device", described in the patent application No. 2007140226/17 of 10.30.2007, was investigated. The characteristics of saline, the concentration of salt components, pH, osmolarity were identical to the first example. The measurement results are shown in table 2.

table 2 Recorded evoked potential of NMDA EPSP neuron receptor The amplitude of the evoked potential NMDA EPSP (μV) The initial amplitude at the time of stimulation 5 min after stimulation 15 min after stimulation 30 min after stimulation 40 min after stimulation NMDA UPU 61 162 193 189 191

The data presented in table 2, during incubation of sections with a solution prepared on test water, indicate that the increase in potential occurs more intensively, that is, the processes of learning and memory formation in nerve cells are significantly enhanced. These results are significantly (U-test, p≤0.05) different from the data presented in table 1. Given that the main characteristics of the incubation solution were the same as in example 1, except for the water itself, previously purified in the device, therefore, the effects of enhancing learning and memory formation are associated with new properties of water after purification.

The proposed method for determining the biological activity of water allows you to determine the effect of water on the learning and memory of nerve cells and compare the biological activity of water from different sources or before and after processing in industrial devices. The method shows that water is a biologically active substance with different levels of activity characteristic of water from different sources.

"Device for water purification" (application for invention No. 2007122226/17) was transferred for research to the Institute of Physiology named after I.P. Pavlova of the Russian Academy of Sciences by the authors Yu.A. Levchenko and Yu.I. Sviridov.

Literature

Bliss T.V.P., Collingridge G.L. // Nature. 1993. V.361. No. 6407. P.31-39.

Claims (1)

A method for determining the biological activity of water, which consists in preparing a standard saline solution from water, preparing surviving preparations of nerve cells, placing cells in this saline solution for 40 minutes, electrical stimulation of cells - 4 bursts of pulses, burst duration 5 s, interval 5 s, suprathreshold intensity, s frequency of 100 Hz, abduction and registration of the initial at the time of stimulation and 5, 15, 30 and 40 min after stimulation of the amplitude of the exciting postsynaptic potential of the N-methyl-D-aspartate receptor and determination of biolo the biological activity of water when comparing the initial parameters of the induced activity of cells during incubation in water, and after its completion, depending on the length of time in the water.