RU2350122C1 - Dietary supplement - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to dietary supplements and can be used in medicine and pharmaceutical industry, in food and cosmetic industry, in agriculture. Supplement contains propolis water extract, propolis spirit extract, propolis glycerine extract and extract of plant raw materials. The components are taken in the following ratio, wt %: propolis water extract (in conversion to dry substances) 2.5-7.7, propolis spirit extract (in conversion to dry substances) 0.45-1.8, propolis glycerine extract (in conversion to dry substances) 12.0-15.0, ethyl alcohol 0.6-5.0, glycerine 8.5-11.5, extract of plant raw materials (in conversion to dry substances) 0.05-0.5 and water up to 100.0. Invention allows to expand spectrum of biological activity and increase food value of dietary supplement.

EFFECT: dietary supplement is assigned to use simultaneously with food as a source of flavonoids and tannins, as bracing mean.

2 cl, 2 ex

Description

The invention relates to propolis-based dietary supplements. The invention can be used in medicine and the pharmaceutical industry, in the food industry, as well as in the cosmetic industry, in agriculture.

Propolis, as a natural product, is a valuable source of biologically active substances.

Representing the natural natural totality of biologically active compounds of plant and animal (bee) origin, propolis has antibacterial, antiviral, antifungal, anti-inflammatory, immunostimulating, regenerative, antioxidant, biostimulating, anesthetic activities.

The biological properties of propolis are largely determined by the phenolic compounds that make up its composition. Most of these compounds have acidic and chelating properties. Many phenolic compounds are antioxidants, i.e. able to extinguish free radical processes. Flavonoids have a P-vitamin effect, are involved in tissue respiration.

It is known that various extracts and extracts (1) used in various preparations of medical and cosmetic directions are prepared from propolis using various solvents.

However, the active principles in various fractions of propolis change, both quantitatively and by nature. This is due to the fact that chemical, incl. phenolic propolis compounds are soluble in various solvents to varying degrees.

Thus, aqueous extracts of propolis, which is its hydrophilic fraction, are distinguished by high qualities in terms of acid and unsaturated compounds, antioxidant, antimicrobial, and antiviral properties compared to other fractions. The short shelf life of aqueous extracts is their drawback.

Alcoholic extracts of propolis, which is its hydrophobic fraction, exhibit more antifungal activity, as well as anesthetic, deodorizing effect.

Glycerin extracts are active against gram-positive and gram-negative microorganisms, have a stimulating effect.

The use of propolis in the composition is known.

Known propolis emulsion (RU 2088224 C1) containing propolis, ethyl alcohol, water, an extract of mineral substances of plant-animal origin and glycerin in the following ratio, wt.%:

Propolis (in terms of solids)  3.0-8.0 Water 71.00-76.45 Ethanol   10.5 Organomineral extract  plant-animal origin (in terms of solids)  0.05-0.5 Glycerol  10.0

Propolis emulsion is made by mixing components, used as a medicine.

A disadvantage of the known propolis emulsion is that it contains native dry propolis, which is introduced into the composition by usual mixing it with other liquid ingredients - water, alcohol, glycerin and with an extract of mineral substances.

However, with such contact of propolis with a mixed alcohol-water-glycerol solvent, the complete dissolution of various propolis fractions is difficult, separate insoluble fractions precipitate, which reduces the content of dissolved biologically active active substances in the finished propolis emulsion and, therefore, limits its biological activity.

Another disadvantage of the known propolis emulsion is a rather high content of ethyl alcohol - 10.5%, which has a toxic effect on the body, especially when taken orally.

The closest analogue of the claimed invention is a suspension (RU 2080132, A61N 1/16, A61K 35/64, 35/78, 47/00) containing propolis, ethyl alcohol, glycerin, water, a mixture of plant materials in the following ratio,%:

Propolis 0.1-1.5 Nettle 0.1-1.5 Yarrow 0.1-1.5 Thyme 0.1-1.5 Motherwort 0.1-1.5 Plantain 0.1-1.5 Coltsfoot 0.1-1.5 Oregano 0.1-1.5 Badan 0.1-1.5 Birch leaves and buds 0.1-1.5 Pine buds 0.1-1.5 Needles of cedar (Siberian pine) 0.1-1.5 Agave 0.1-1.5 Nutmeg 0.1-1.5 Chamomile 0.1-1.5 Immortelle 0.1-1.5 Hairpin 0.1-1.5 Sophora Japanese 0.1-1.5 St. John's wort 0.1-1.5 Mint 0.1-1.5 Hawthorn 0.1-1.5 Dry residue of all vegetable components 0.1-1.5 Ethanol 7-38 Glycerol 3-8 Water up to 1 l

The dry residue of the finished suspension is 0.1-1.5%.

A disadvantage of the known suspension is its lack of biological activity and nutritional value. This is due to the low percentage of dry matter, incl. propolis, and, therefore, a small amount of biologically active substances in suspension. In addition, propolis in a known suspension is contained only in the form of an alcoholic extract, which is its hydrophobic fraction. The absence in the suspension of compounds of other phenolic fractions of propolis, including hydrophilic, limits the manifestation in it of the total biologically active principle of propolis, i.e. spectrum of its biological activity.

Another disadvantage of the known suspension is a rather high content of ethyl alcohol - up to 38%, which has a toxic effect on the body, especially when taken orally, thereby excluding the use of the suspension as a medicine by persons who have contraindications to alcohol-containing products.

The objective of the invention is to expand the spectrum of biological activity, increase the nutritional value of the created product - biologically active additives (hereinafter - dietary supplements), expand the range of potential consumers of dietary supplements, as well as expand the arsenal of biologically active additives.

The problem is solved in that the proposed biologically active additive containing propolis, water, glycerin, ethyl alcohol, a mixture of plant materials, contains propolis water extract, propolis alcohol extract, propolis glycerin extract as a propolis, and a biologically active additive as a plant material mixture contains plant extract, in the following ratio of ingredients, wt.%:

Propolis aqueous extract (in terms of solids) 2.5-7.7 Propolis alcohol extract (in terms of solids) 0.45-1.8 Propolis glycerin extract (in terms of solids)  12.0-15.0 Ethanol 0.6-5.0 Glycerol 8.5-11.5 Extract of plant materials (in terms of solids) 0.05-0.5 Water  up to 100.0

As plant materials for plant material extracts, plants from the following series can be used in equal proportions: nutmeg, nettle (leaves), oregano (grass), chamomile (flowers), incense (rhizomes), coltsfoot (leaves), plantain (leaves), motherwort (grass), thyme (grass), pine (needles, buds), St. John's wort (grass), birch (buds), yarrow (grass).

Introduction to the proposed biologically active additive as propolis of its extracts - water, glycerin, alcohol - provides the presence in it of various groups of (hydrophilic and hydrophobic) phenolic propolis compounds, including: flavones - chrysin, apigenin, acacetin; flavonols - quercetin, kempferol, isoramnetin, galangin; flavonoids - routine; hydroxycinnamic acids - ferulic, caffeic, chlorogenic; tannins - tannins.

Thus, the spectrum of the biological potential of propolis is manifested in the proposed dietary supplement to the widest and fullest extent.

The extract of plant raw materials introduced into the composition of the biologically active additive complements the sum of biologically active compounds characteristic of propolis - tannins - due to buds of birch and pine, hydroxycinnamic acids - due to nettles, flavones - due to chamomile, and also supplements dietary supplements with other compounds characteristic of plant materials used. So, the presence of flavonoids such as hyperoside, rutin, kempferid, astragaline, dimethyl camperol in the dietary supplement is due to the use of St. John's wort, thyme, oregano, motherwort, yarrow, and the presence of arbutin and hydroquinone is associated with the use of thick frangipani and plantain large. The presence of polysaccharides in dietary supplements is mainly due to the use of coltsfoot and plantain.

This combination of compounds provides an additional extension of the spectrum of biological activity of the claimed dietary supplement, since different flavonoids give various additional effects, for example, hyperoside has a hypoazotemic property, rutin has a capillary-strengthening property, arbutin has diuretic, and hydroquinone has bactericidal properties. Polysaccharides have a positive effect on metabolism. Nutmeg as a spice improves digestion.

Thus, the qualitative composition of the proposed biologically active additives provides a wide range of its biological activity.

Quantitative percentages of the components of a biologically active additive also affect the technical result achieved, since it has been experimentally established that it is precisely in the indicated quantitative ranges that the required high nutritional value of dietary supplements is provided (i.e., a high level of the content of the main active ingredients in dietary supplements), the range of potential consumers is expanding Supplements, while maintaining its stability.

So, the water-soluble propolis fraction has a quantitative (2.5-7.7 wt.%) Advantage over the hydrophobic fraction (0.45-1.8 wt.%). Because of this, the qualitative phenolic composition of the proposed biologically active additives is represented mainly by acidic unsaturated compounds, including hydroxycinnamic acids, which cause the high antioxidant ability of the proposed dietary supplement.

At the same time, the hydrophobic fractions in the dietary supplement are contained in the amount that is maximum possible for their complete dissolution in the indicated amount (0.6-5.0 wt.%) Of an acceptable solvent - ethyl alcohol. At the same time, the low content of the toxic component - ethyl alcohol - solves the problem of expanding the circle of potential consumers - people who can use the proposed dietary supplement without professional and age restrictions.

The indicated amount of dry glycerin extract of propolis (12.0-15.0) makes up for the lack of tannins in dietary supplements due to the low content of hydrophobic propolis fraction in dietary supplements.

Solvents - water, ethyl alcohol, glycerin - were taken in minimal quantities sufficient to completely dissolve the indicated amounts of the corresponding fractions of propolis and plant materials. That is, the quantitative ratios of “dry extract: acceptable solvent” are such that an increase in the proportion of dry extract (respectively, a decrease in the proportion of solvent) in any of the ratios of “dry extract: acceptable solvent” leads to incomplete dissolution of the dry matter, precipitation of insoluble precipitate. A decrease in the proportion of dry extract (respectively, an increase in the proportion of solvent) in any of the ratios “dry extract: acceptable solvent” leads to a decrease in the content of biologically active components in dietary supplements and, therefore, to a decrease in its nutritional value. At the same time, the presence of the amount of glycerol indicated in the dietary supplement (8.5-11.5 wt.%) Is sufficient to give (in combination with a small ethyl alcohol content of not more than 5.0%) the claimed biologically active additive of stability, which provides a rather long shelf life its storage - 3 years.

A dietary supplement is prepared by mixing the components, it is preferable to mix the dry extracts with appropriate solvents, followed by combining the resulting solutions in any order.

The dietary supplement is a liquid with a characteristic propolis odor, a specific bitter-sweetish, tart taste, from light beige to brown in color. The dietary supplement is intended for use simultaneously with food as a source of flavonoids, hydroxycinnamic acids and tannins, as a general strengthening agent.

Examples of the preparation of biologically active additives.

Example 1. Dietary supplement of the following composition, wt.%:

Propolis aqueous extract (in terms of solids) 2.5 Propolis alcohol extract (in terms of solids) 0.45 Propolis glycerin extract (in terms of solids) 12.0 Ethanol 0.6 Glycerol 8.5 Extract of plant materials (in terms of solids) 0.05 Water  up to 100.0

In this example, a dietary supplement was prepared by mixing the indicated amounts of components in the following order: dry propolis aqueous extract, dry propolis alcohol extract, propolis dry glycerin extract, each separately, mixed with solvents - water, ethyl alcohol, glycerin, respectively, until they were completely dissolved, after which, in the prepared aqueous solution of propolis was introduced, stirring, in succession, a glycerin solution of propolis, an alcohol solution of propolis, an extract of vegetable cheese rd.

The obtained biologically active additive is a liquid with a characteristic propolis odor, a specific bitter-sweetish, tart taste, and beige.

Example 2. Dietary supplement of the following composition, wt.%:

Propolis aqueous extract (in terms of solids) 7.7 Propolis alcohol extract (in terms of solids) 1.8 Propolis glycerin extract (in terms of solids) 15.0 Ethanol 5,0 Glycerol 11.5 Extract of plant materials (in terms of solids) 0.5 Water  up to 100.0

In this example, a dietary supplement was prepared by mixing the indicated amounts of components in the following order: dry propolis aqueous extract, propolis dry alcohol extract, propolis dry glycerin extract, each separately, mixed with solvents - water, ethyl alcohol, glycerin, respectively, until they were completely dissolved, after which, in the prepared glycerol solution of propolis was introduced, stirring, successively, an alcohol solution of propolis, an extract of plant materials, an aqueous solution of propol sa.

The obtained biologically active additive is a liquid with a characteristic propolis odor, a specific bitter-sweetish, tart taste, and brown.

The results of analytical studies of the claimed dietary supplement confirm its high biological activity and nutritional value. So, the actual content of the main active ingredients in dietary supplements is: the sum of flavonoids in terms of rutin is not less than 0.1%, hydroxycinnamic acids - not less than 0.2%, tannins in terms of tannin - not less than 1.0%. Based on this, the actual intake of flavonoids, hydroxycinnamic acids, tannins from dietary supplements into the body is at least 10-15, 60-90, 15-23% of the recommended level of consumption in the daily diet, respectively. The solids content in the dietary supplement is at least 15.0%.

The results of sanitary-chemical, microbiological, radiological studies confirm that the claimed dietary supplement in terms of safety meets the requirements of SanPiN 2.3.2.1078-01, i.e. harmless and non-toxic.

A study of the clinical effectiveness of dietary supplements was conducted at the Novosibirsk State Medical Academy of 93 volunteers who had a history of effectively treated infectious diseases (acute intestinal infections), 4 days after discharge from the hospital. Persons of the main group (58 people) took dietary supplements of 20-30 drops 3 times a day for 40 days. The examined comparison groups (35 people) did not take dietary supplements. In the process of testing, clinical, laboratory and instrumental studies were conducted.

The results obtained in the study of dietary supplements indicate its good tolerability. In 55% of patients under the influence of dietary supplements there was an increase in physical activity, a decrease in fatigue, in 27% - an improvement in appetite, in 22% - a laxative effect, in 56% - an improvement in sleep, on the SAN scale - an improvement in overall well-being, activity, and mood.

The test results of the proposed dietary supplement passed expert assessment at the State Research Institute of Nutrition RAMS for the purpose of state registration, on the basis of which the dietary supplement is recommended as a source of flavonoids, hydroxycinnamic acids and tannins, as a general strengthening agent.

The claimed invention extends the range of biologically active additives in liquid form.

Thus, by the combination of the distinctive features of the proposed dietary supplement, namely, its qualitative and quantitative composition, the indicated technical result of the invention is achieved - expanding the spectrum of biological activity and increasing the nutritional value of the created dietary supplement, expanding the circle of potential consumers of dietary supplements, expanding the arsenal of biologically active additives.

Information sources

1. Vakhonina T.V. Bee pharmacy. - 2nd ed., Revised. and add. - St. Petersburg: Lenizdat, 1995, p. 147.

2. RU 2080132 C1, 05.27.97.

3. RU 2088224 C1, 08.27.97.

Claims (2)

1. A biologically active additive containing propolis, water, glycerin, ethyl alcohol, a mixture of plant materials, characterized in that as propolis a biologically active additive contains an aqueous extract of propolis, an alcoholic extract of propolis, a glycerin extract of propolis, as a mixture of plant materials a biologically active the additive contains an extract of plant materials in the following ratio of components, wt.%:
propolis aqueous extract (in terms of solids) 2.5-7.7 propolis alcohol extract (in terms of solids) 0.45-1.8 propolis glycerin extract (in terms of solids) 12.0-15.0 ethanol 0.6-5.0 glycerol 8.5-11.5 plant extract (in terms of solids) 0.05-0.5 water up to 100.0 2. The dietary supplement according to claim 1, characterized in that as a plant material for an extract of plant material used in equal proportions of a plant from a number: nutmeg, nettle (leaves), oregano (grass), chamomile (flowers), incense ( rhizomes), coltsfoot (leaves), plantain (leaves), motherwort (grass), thyme (grass), pine (needles, buds), St. John's wort (grass), birch (buds), yarrow (grass).