RU2333769C1 - Vaccine for prevention of coenurosis and cysticercosis in farm animals - Google Patents

Abstract

FIELD: medicine, veterinary.

SUBSTANCE: invention concerns veterinary parasitology. The vaccine includes 2 day culture of Multiceps multiceps larvocysts, 2 day culture of Taenia hydatigena larvocysts, medium 199, cattle blood serum, benzylpennicillin potassium salt, streptomycin sulfate, formalin and aluminum hydroxide at the following component ratio (volume %): 2 day culture of Multiceps multiceps larvocysts - 1.1·10³-1.2·10³ specimen per ml, 2 day culture of Taenia hydatigena larvocysts - 1.1·10³-1.2·10³ specimen per ml, cattle blood serum - 8.0-10.0, benzylpennicillin potassium salt - 0.018-0.02, streptomycin sulfate - 0.029-0.03, formalin - 8.0-10.0, aluminum hydroxyde - 8.0-10.0, the rest being medium 199.

EFFECT: obtaining harmless vaccine effective against coenurosis and cysticercosis in farm animals, displaying high immunizing power and long storage term.

3 ex

Description

The invention relates to the field of veterinary parasitology.

The problem of coenurosis in livestock farms in Russia and many other countries has not lost its relevance to this day. Coenurosis infestations are diseases of sheep, goats, cattle, less often other ungulates and rodents. Almost all animals infected with coenurosis die, and forcedly slaughtered animals give poor-quality meat. Therefore, the main task is to sharply reduce this invasion, and then its complete elimination-devastation by creating in nature such conditions under which the causative agents of this invasion could not exist. The main method of combating animal coenurosis is their immunization. The results of immunization prove that in the first year of its use, the number of diseased animals decreases by 15-20 times. In the second year of its use, the number of sick animals decreases to 0.5-0.01% of the total number of young animals, and in some cases to the complete absence of sick animals. The third year of immunization of newborn animals is usually characterized by the absence of invasive animals among them. Infectious dogs, which are the main source of infection, are then self-released from the pathogen 6-7 months after infection. Re-infection of dogs with multi -ceptosis does not occur due to the absence of coenosis animals on farms.

Thus, the use of an anti-cenosis vaccine in livestock farms located in different climatic zones, with different methods of housekeeping and a different epizootic situation for coenurosis, is a powerful technique for controlling and preventing this invasion.

A vaccine for the prevention of larval teniidosis of farm animals is known, containing a two-day culture of larvocyst Multiceps multiceps, a nutrient medium based on medium 199 and blood serum, benzylpenicillin potassium salt, streptomycin sulfate, merthiolate and aluminum hydroxide (see, for example, SU 1237214 A1, 06/15/1986 )

A vaccine for the prevention of tenuicolol cysticercosis in sheep is known, containing a two-day culture of oncospheres Taenia hydatigena, a nutrient medium based on medium 199 and blood serum, benzylpenicillin potassium salt, streptomycin sulfate, merthiolate and aluminum hydroxide (see, for example, RU 94028803 A, 20.10.1996 )

A disadvantage of the known vaccines is their narrow spectrum of action, low immunogenicity and short shelf life.

Known vaccine for the prevention of cysticercosis of farm animals, obtained by genetic engineering, based on two types of protein found in the oncospheres. Two vaccinations given at 30-day intervals give immunity lasting several months. The effectiveness of the vaccine is 99.5-100% (see, for example, https://elementy.ru/news/164614, 07/13/2005).

However, the method of obtaining this vaccine is complex, and its immunogenicity is low.

Vaccines effective against both coenurosis and thin-necked cysticercosis of farm animals are not known in the art.

An object of the present invention is to provide a vaccine effective against both coenurosis and thin-necked cysticercosis of farm animals, harmless, with high immunogenicity and a long shelf life.

The problem is solved due to the fact that according to the invention, the vaccine for the prevention of coenurosis and thin-necked cysticercosis of farm animals contains a two-day culture larvocyst Multiceps multiceps and a two-day culture larvocyst Taenia hydatigena, medium 199, serum of cattle, benzylpenicillin potassium salt, streptomycin aluminum hydroxide in the following ratio of ingredients (in vol.%):

two-day culture larvocyst

Multiceps multiceps 1.1 · 10 ³ -1.2 · 10 ³ ind./ml

two day culture

larvocyst Taenia hydatigena 1.1 · 10 ³ -1.2 · 10 ³ ind./ml cattle blood serum 8.0-10.0 benzylpenicillin potassium salt 0.018-0.02 streptomycin sulfate 0,029-0,03 formalin 8.0-10.0 aluminum hydroxide 8.0-10.0 Wednesday 199 rest

The technical result achieved by the invention is that the vaccine obtained is harmless, has a long shelf life, high immunogenicity and protects farm animals from infection by both coenurosis and thin-necked cysticercosis.

The invention is illustrated by the following examples.

Example 1. Getting the vaccine

Dogs are invaded by multiceps multiceps and Taenia hydatigena chain larvae (hereinafter, M. multiceps and T. hydatigena, respectively). Then deworming experimentally invaded dogs. Wash M. multiceps and T. hydatigena in tap water. Mature segments of M. multiceps and T. hydatigena are separated. Mature joints are destroyed with the subsequent washing of eggs in tap water. The eggs of M. multiceps and T. hydatigena are separately sequentially incubated in gastric juice for 1 hour, and then in intestinal juice for 15 minutes at a temperature of 37 ° C. The composition of the gastric juice (pH 1.5-2.0): pepsin (medical) - 1.0 g, hydrochloric acid - 1.0 ml, distilled water - up to 100.0 ml. The composition of intestinal juice (pH 7.1-7.3): pancreatin - 1.0 g, cattle bile - 10.0 ml, distilled water - up to 100.0 ml.

The oncospheres freed from the egg shells are washed from the remains of the intestinal juice by the cultivation medium, after which the oncospheres are transferred to a fresh portion of the cultivation medium and placed in a thermostat for 48 hours at a temperature of 37 ° C. During the 48-hour cultivation, oncospheres develop into larvocysts. The culture medium is prepared from (vol.%): Blood serum of cattle - 8.0, benzylpenicillin potassium salt - 0.018, streptomycin sulfate - 0.029, environment 199 - the rest.

The number of larvocysts is calculated separately for each type of pathogen. The number of M. multiceps larvocysts was adjusted to 1.1 · 10 ³ ind./ml and T. hydatigena to 1.1 · 10 ³ ind./ml, taking into account the subsequent addition of formalin (8.0 vol.%) And aluminum hydroxide (8 , 0 vol.%).

Prepared according to the described methodology, cultures with larvocysts are mixed in equal volumes. The finished vaccine, with constant stirring, is poured into 200 ml vials, controlled for sterility and safety.

Example 2

The vaccine is prepared according to example 1, but the washed eggs of M. multiceps and T. hydatigena after the destruction of the mature joints are separately sequentially kept in gastric juice for 1.5 hours, and in intestinal juice for 20 minutes at a temperature of 38 ° C. Oncospheres washed from the remains of intestinal juice are placed in a thermostat for 48 hours at a temperature of 38 ° C. The cultivation medium contains (vol.%): Blood serum of cattle - 10.0, benzylpenicillin potassium salt - 0.02, streptomycin sulfate - 0.03, medium 199 - the rest. Bring the number of M. multiceps larvocysts to 1.2 · 10 ³ ind./ml and T. hydatigena to 1.2 · 10 ³ ind./ml, taking into account the subsequent addition of formalin (10.0 vol.%) And aluminum hydroxide (10 , 0 vol.%).

Prepared according to the described methodology, cultures with larvocysts are mixed in equal volumes. The finished vaccine, with constant stirring, is poured into 250 ml bottles, controlled for sterility and harmlessness.

Example 3

Lambs (10 animals) and calves (10 animals) were immunized with the vaccine. Young farm animals are immunized no earlier than 1.5 months of age, twice with an interval of 10-12 days. The vaccine is administered intramuscularly from the inside of the thigh at a dose of 1 ml per head.

Immunity is checked by experimental infection of immunized animals.

The immunogenicity of the vaccine is controlled by the reaction of latex agglutination (RLA) by the titer of antibodies in the blood serum of experimental animals. The antibody titer is considered satisfactory with positive RLA results when the serum is diluted 1:32; 1:64.

The results of the study showed that the vaccine retains immunogenicity for at least 1.5 years at a storage temperature of 6-10 ° C.

The duration of immunity in immunized animals is 1 year or more.

Thus, the vaccine obtained is effective both against coenurosis and against thin-necked cysticercosis of farm animals, is harmless, has high immunogenicity and a long shelf life.

Claims (1)

Vaccine for the prevention of coenurosis and thin-necked cysticercosis of farm animals, containing a two-day culture larvocyst Multiceps multiceps culture, a two-day culture larvocyst Taenia hydatigena, medium 199, serum of cattle, benzylpenicillin potassium salt, streptomycin sulfate, aluminum formalin and hydroxide .%: two-day culture larvocyst Multiceps multiceps 1.1 · 10 ³ -1.2 · 10 ³ ind./ml two day culture larvocyst Taenia hydatigena 1.1 · 10 ³ -1.2 · 10 ³ ind./ml cattle blood serum 8.0-10.0 benzylpenicillin potassium salt 0.018-0.02 streptomycin sulfate 0,029-0,03 formalin 8.0-10.0 aluminum hydroxide 8.0-10.0 Wednesday 199 rest.