RU2321390C2 - Medicinal composition for preparing intrauterus suppository - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention proposes a composition comprising the following agents, wt.-%: liposomal concentrate emulsion with antibiotic of aminoglycoside class, 28.5-29.8; mother milk, 1.5-2.8; propolis, 2.3-4.2; bee wax, 22.5-24.1; hydrogenated fat, 23.5-25.0; gelatin, 1.5-2.0, and glycerol as an emulsifier, the balance. Suppository based on the proposed composition possesses the prolonged effect, low toxicity and high effectiveness.

EFFECT: improved and valuable medicinal properties of composition.

2 tbl, 4 ex

Description

FIELD OF THE INVENTION

The invention relates to veterinary medicine, in particular to intrauterine suppositories for the treatment of farm animals.

State of the art

Known antibacterial agent containing streptomycin, included in liposomes, the membrane of which is formed on the basis of lecithin (see Vladimirsky N.A., Ladygina G.A., Tentsova A.I. "Antibiotics", No. 1, 1983, p.23- 26).

The disadvantage of this tool is its low efficiency in the treatment of diseases of bacterial etiology.

A known basis for suppositories containing hydrogenated cottonseed oil, an emulsifier, while it additionally contains gelatin, distilled water, as an emulsifier glycerin in the following ratio of ingredients, wt.%:

Hydrogenated Cottonseed Oil 20-30 Gelatin 8-12 Distilled water 16-25 Emulsifier-Glycerin rest

(see AS of the USSR No. 1650139, class A61K 9/02, published on May 23, 1991). The disadvantage of this basis for suppositories is the low absorption of the active substance.

The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is an antibacterial agent for treating diseases of farm animals, which is an emulsion of liposomes with an antibiotic of the class of aminoglycosides included in them in an effective amount, while the liposome membrane contains lipids extracted from organs and tissues of large cattle (see Pat. RF №2158587, class. A61K 9/127, 31/7036, 35/12, A61P 31/04, publ. 10.11.2000).

The disadvantage of this antibacterial agent is not high enough prolonged action and relatively high toxic effects on the body.

Disclosure of invention

The task of the invention is the development of intrauterine suppositories for the treatment of farm animals with prolonged action, low toxicity and high antibiotic therapy, reducing the duration of the therapeutic course and the frequency of administration of suppositories.

The technical result that can be achieved using the present invention is to ensure the prolonged action of the drug, reduce its toxic effects, increase the effectiveness of antibiotic therapy, including intracellular infection, preservation from biodegradation, reduce the duration of the therapeutic course and the frequency of administration of suppositories.

The technical result is achieved using intrauterine suppositories for farm animals, including an emulsion of liposomal concentrate with an antibiotic of the aminoglycoside class included in them in an effective amount, gelatin, an emulsifier, which is glycerol, and royal jelly, propolis, and beeswax are added to it, hydrogenated fat in the following ratio of components in wt.%:

Liposome Concentrate Emulsion with aminoglycoside class antibiotic 28.5-29.8 Royal jelly 1.5-2.8 Propolis 2,3-4,2 Beeswax 22.5-24.1 Hydrogenated fat 23.5-25.0 Gelatin 1.5-2.0 Emulsifier-Glycerin rest

The essence of the preparation of intrauterine suppositories for the treatment of farm animals is as follows.

A predetermined amount of the base for suppositories is placed in the container, namely beeswax, hydrogenated fat, gelatin, emulsifier, which is used as glycerin, heated to a temperature of 35-40 ° C with constant stirring, add an emulsion of liposome concentrate with an antibiotic of the aminoglycoside class, royal jelly and propolis, the temperature was adjusted to 45-53 ° C, the mixture was homogenized for 25-30 minutes, cooled to a temperature of 28-29 ° C, Packed and stored in a refrigerator at a temperature of 4-6 ° C. The resulting intrauterine suppositories have a solidification temperature of 29 ° C, a melting point of 36-38 ° C. Suppositories are yellowish-white in color with a faint specific odor, solid at room temperature.

Intrauterine suppositories according to the invention have a prolonged action, low toxicity and high antibiotic therapy effectiveness due to the fact that the emulsion of liposomal concentrate provides the possibility of intracellular drug delivery, increases the duration of the antibiotic, prevents its destruction, royal jelly has bacteriostatic and bactericidal properties, antiviral, antitoxic , tonic and stimulating effects, propolis has an thymicobic, anti-inflammatory, anesthetic, enhances the regeneration processes and increases the immune status of animals, beeswax, hydrogenated fat, gelatin and emulsifier-glycerin is the basis for suppositories and has high plasticity (see Kravtsov M.I., Lebedev V.I. "Products of beekeeping", 1995, p.251).

The implementation of the invention

Examples of specific production of intrauterine suppositories for the treatment of farm animals.

Example 1. In the container is placed, in wt.%, The basis for suppositories, namely: beeswax 20.5, hydrogenated fat 22.5, gelatin 1.0, emulsifier-glycerin - the rest after calculating the ratio of components, heated to a temperature of 25 -30 ° C with constant stirring, add an emulsion of liposome concentrate - 25.5 with an antibiotic of the aminoglycoside class, royal jelly 1.0, propolis 2.0, the temperature was adjusted to 40 ° C, the mixture was homogenized for 15 minutes, cooled to a temperature 25 ° C.

The obtained base for suppositories is yellowish with a weak specific odor, has a solidification temperature of 15.5 ° C and is at room temperature in a mild state, when tested on animals it did not give a high therapeutic effect, namely, the treatment period is long - up to 30 days with a frequency of administration suppository up to 3 times a day.

Example 2. Obtaining the basis for intrauterine suppositories is carried out analogously to example 1, but the ratio of components, in wt.%, Is taken in the following amount:

Liposome Concentrate Emulsion with aminoglycoside class antibiotic 28.5 Royal jelly 1,5 Propolis 2,3 Beeswax 22.5 Hydrogenated fat 23.5 Gelatin 1,5 Emulsifier-Glycerin rest

the suppository base is heated to a temperature of 35 ° C with constant stirring and after adding an emulsion of liposome concentrate, an antibiotic of the aminoglycoside class, royal jelly and propolis, the temperature is brought to 45 ° C, the mixture is homogenized for 25 minutes, cooled to 28 ° C, Packed and stored in a refrigerator at a temperature of 4-6 ° C.

The obtained intrauterine suppositories of a yellowish-white color with a weak specific odor have a solidification temperature of 28 ° C, solid at room temperature, a melting point of 36-38 ° C, showed a high therapeutic effect during the test, namely, the treatment period was significantly reduced - up to 5 days with a frequency of administration of a suppository 1 time per day.

Example 3. Obtaining the basis for intrauterine suppositories is carried out analogously to examples 1, 2, but the ratio of components, in wt.%, Is taken in the following amount:

Liposome Concentrate Emulsion with aminoglycoside class antibiotic 29.8 Royal jelly 2,8 Propolis 4.2 Beeswax 24.1 Hydrogenated fat 25.0 Gelatin 2.0 Emulsifier-Glycerin rest

the base for suppositories is heated to a temperature of 40 ° C with constant stirring and after adding an emulsion of a liposome concentrate of an antibiotic of the aminoglycoside class, royal jelly and propolis, the temperature is brought to 53 ° C, the mixture is homogenized for 30 minutes, cooled to 29 ° C, Packed and stored in a refrigerator at a temperature of 4-6 ° C.

The obtained suppositories, as in example 3, are yellowish-white in color, with a weak specific odor, have a solidification temperature of 29 ° C, remain solid at room temperature, a melting point of 36-38 ° C, during the test they also showed a short treatment time - up to 5 days with a frequency of administration of a suppository up to 1 time per day, lack of toxicity.

Example 4. Obtaining the basis for suppositories is carried out analogously to examples 1, 2, 3, but the ratio of components, in wt.%, Is taken in the following amount:

Liposome Concentrate Emulsion with aminoglycoside class antibiotic 30,0 Royal jelly 3.0 Propolis 5,0 Beeswax 25.0 Hydrogenated fat 25.5 Gelatin 2,5 Emulsifier-Glycerin rest

the suppository base is heated to a temperature of 50 ° C with constant stirring, and after adding an emulsion of a liposome concentrate with an antibiotic of the aminoglycoside class, royal jelly and propolis, the temperature is brought to 57 ° C, the mixture is homogenized for 30 minutes, cooled to 30 ° C .

The obtained suppositories, as in example 3, are yellowish-white in color, with a weak specific odor, have a solidification temperature of 29 ° C, remain solid at room temperature, a melting point of 36-38 ° C, during the test they also showed a short treatment time - up to 5 days with a frequency of administration of a suppository up to 1 time per day, the absence of toxicity, but the consumption of components increased, the cost of the drug increased.

Thus, the most optimal are examples 2 and 3, since the obtained intrauterine suppositories during the test showed a high therapeutic effect and the frequency of administration of suppositories. The test results are shown in tables 1, 2. Table 1 shows the results of treatment with furazolizone suppositories, and in table 2 according to the invention.

Table 1 No. P. Kind of animal Number of belly., Goals Duration of treatment, day Multiplicity of introduction Number of nonsense. animal Effective treatment% one. Cattle 23 thirty 5 17 73.9

table 2 No. P. Kind of animal Number of animals. Goals Duration of treatment, day Multiplicity of introduction Number of nonsense. animal Effect. treatment% one Cattle 21 5 one twenty 95.2

The invention in comparison with the prototype and other known technical solutions has the following advantages:

- providing a prolonged action of the suppository due to the inclusion of an emulsion of liposome concentrate;

- reduction of toxic effects;

- increasing the effectiveness of antibiotic therapy, including intracellular infection, due to targeted delivery of the drug to the pathogen;

- reducing the duration of the therapeutic course and the frequency of administration of suppositories;

- Preservation of the suppository from biodegradation.

Claims (1)

A medicinal composition for the preparation of intrauterine suppositories, characterized in that it contains an emulsion of a liposome concentrate with an antibiotic of the aminoglycoside class, royal jelly, propolis, beeswax, hydrogenated fat, gelatin and emulsifier-glycerin in the following ratio, wt.%: liposomal emulsion antibiotic concentrate class of aminoglycosides 28.5-29.8 royal jelly 1.5-2.8 propolis 2,3-4,2 beeswax 22.5-24.1 hydrogenated fat 23.5-25.0 gelatin 1.5-2.0 emulsifier glycerin rest