RU2281721C1 - Eqiupment for obtaining uterine content in cows for bacteriological trials - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the suggested equipment consists of a body with a piston and plunger inside, a plastic tube and sterile rubber case. The tube is fixed to the body with the help of cannula and a rubber hose. The case covers the tube and is designed with possibility to be broken due to its tension along the length of the equipment suggested. The innovation enables to aseptically obtain uterine content right from uterine cavity and increase significance of the results of bacteriological investigations.

EFFECT: higher efficiency.

2 dwg

Description

The invention relates to the field of veterinary medicine, in particular to devices for collecting uterine contents from cows for bacteriological studies.

The inventive device is used to take the contents of the uterus to determine the species composition of microflora at various times in the postpartum period and to determine the sensitivity of the isolated microflora to antimicrobial agents in order to correctly select an antimicrobial drug for treatment. The inventive device can also be used to obtain uterine contents and after the expiration of the postpartum period to determine the nature of the course of a pathological process.

The analogue is known - taking the contents of the vagina or prepuce in animals with cotton swabs. For these purposes, cotton swabs are prepared on a stick, which are placed in a test tube containing 4-5 ml of pH-7.4 meat-peptone broth (the broth swab does not apply). The tube is closed with a cotton-gauze plug fixed on the same stick and sterilized for 45 minutes at a pressure of 0.8 atm. in the autoclave. When taking a sample, the test tube is quickly opened and with a cotton swab, trying not to touch the external integument, make a smear from the vagina or prepuce. Then the tube is closed, the swab is moistened in the broth, after which the tube is placed in a thermostat at a temperature of 35-37 ° C / 1 /. The disadvantage of the described method of taking is that with the help of cotton swabs you can get only the contents of the vagina or prepucial bag of animals, but not the uterus, and therefore the diagnostic value of bacteriological studies to clarify the nature of the pathological process in the uterus is reduced.

A well-known analogue is the method of taking the contents of the vagina in women using a disposable cotton swab. The selection of the contents of the vagina is carried out with a disposable swab, which, after being taken, is delivered in a dry container to the bacteriological laboratory within 2 hours / 2 /. The disadvantage of this method of taking contents for conducting microbiological studies is that in the process of taking the possibility of microbial contamination of the tampon is not excluded, and therefore the reliability of the results of bacteriological studies is reduced.

A known analogue is the taking of the contents of the vagina and cervix in women with a disposable syringe / 3 /. The test material from the cervical canal and separately from the vagina was taken with a sterile disposable syringe in a volume of 0.1 ml of secretion into test tubes with 0.9 ml of 1% peptone water and delivered to the laboratory within an hour from the moment the material was taken. The disadvantage of this method of capture is the inability to use it to capture the uterine contents directly from the uterine cavity.

The closest technical solution is a device for obtaining uterine-cervical secretions in cows for bacteriological studies / 4 /. The device consists of polyethylene pipettes and single-use ampoules, which are intended for artificial insemination. These pipettes and ampoules come in sterile form in plastic bags. A sterile saline solution is collected in both ampoules over the flame of the spirit lamp, one ampoule is connected to a pipette and the saline solution is sucked into it, after which the second ampoule is put on. The device is ready to go. After sanitary treatment of the external genitalia, a vaginal mirror is inserted, the ampoule is disconnected from the device and it is promoted without touching the vaginal mucosa into the cervix. Then saline is squeezed and the contents of the cervical canal are sucked in. The device is removed from the genitals, the end is wiped with a cotton swab moistened with alcohol, an ampoule is attached and the material is delivered to the laboratory.

The disadvantage of this device is that with it you can not get the contents directly from the uterus, which affects the reliability of the results of bacteriological studies.

The technical result of the invention is aseptic obtaining uterine contents directly from the uterine cavity, increasing the reliability of the results of bacteriological studies.

This goal is achieved using a device for taking the contents of the uterus from cows for bacteriological studies.

The inventive device for receiving the contents of the uterus from cows for bacteriological studies (Figure 1) contains a plastic tube 1 with a length of 450 mm, which is connected through a piece of rubber hose 2 and cannula 3 to the body of the device 4. Inside the body of the device there is a piston 5 moving inside the body 4 by means of a stem 6. A plastic tube 1, a rubber adapter 2 and a cannula 3 are placed in a sterile rubber case 7 (a condom can be used as such).

The device operates as follows. In the claimed device for obtaining the contents of the uterus in cows after sterilization over a flame of an alcohol lamp, a sterile physiological solution is collected in the housing 4 using a piston 5, then placed in a sterile rubber cover 7 and stretched along the entire length of the device. The device in this form is ready for use (Figure 1). After sanitization of the external genitalia, a vaginal mirror is inserted through which the device is advanced. Under the control of the operator’s arm inserted into the rectum, they are advanced through the cervix into the cavity of one of the horns. After the end of the plastic tube 1 will be in the cavity of one of the horns of the uterus, it is necessary to break through the rubber cover 7 by pulling it along the length of the device, use the piston rod 6 to remove saline from the body of the device 4 and suck the contents of the uterus. Next, remove the device from the genitals (Figure 2), wipe the end of the tube 1 with cotton moistened with alcohol, place the device in a new sterile rubber cover 7 and, in this form, deliver it to the laboratory.

Information sources

1. Ignatov P.E. Essays on infectious diseases of dogs. M .: Valta, 1995 .-- S.53-54.

2. Boyko A.V., Aleshkin V.A., Afanasyev S.S. et al. Microflora of the vagina in prepubertal girls // Microbiology, Epidemiology and Immunology. - 2001. - No. 2. - S. 91-94.

3. Boyko A.V., Aleshkin V.A., Afanasyev S.S. et al. Microflora of the vagina in prepubertal girls // Microbiology, Epidemiology and Immunology. - 2001. - No. 2. - S. 91-94.

4. Boyko A.V., Aleshkin V.A., Afanasyev S.S. et al. Microflora of the vagina in prepubertal girls // Microbiology, Epidemiology and Immunology. - 2001. - No. 2. - S. 91-94.

Claims (1)

A device for receiving the contents of the uterus from cows for bacteriological research, consisting of a body, inside of which there is a piston with a rod, a plastic tube attached to the body using a cannula and a rubber hose, as well as a sterile rubber cover that covers the tube and is made to break through by tension its along the length of the device.

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