RU2280423C1 - Method for treating dogs at iron deficiency anemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: it is necessary to introduce bioferon intramuscularly at the dosage of 1 ml/40 kg body weight for 5 d. Additionally, it is necessary to affect symmetric biological active points (BAP) of hepatic and splenic meridians Liv₂, Liv₃, Liv₁₄, SP₆, SP₉, SP₁₀. One should affect with alternating current of 10-15 mcA, duration being 60 sec for 5 d. One should measure BAP electroconductivity at positive and negative polarities to compare them. If positive polarity value dominates it is necessary to affect BAP with positive potential and if negative polarity dominates one should affect BAP with negative potential. Impact lasts till the difference in electroconductivity values escapes and then one should diagnose recovery in animals. The innovation decreases labor capacity and time expenses for therapy implementation.

EFFECT: higher efficiency of therapy.

2 ex, 2 tbl

Description

The invention relates to veterinary medicine, in particular to therapy, and can be used to treat dogs with iron deficiency anemia.

Currently, a number of methods and tools are known that are used for iron deficiency anemia. Of the drugs, the most widely used preparations are iron, copper, cobalt, campolon, hemostimulin. However, the use of these pharmacological agents that affect various functions of the body is significantly limited due to the development of undesirable side effects. Therefore, in recent years, the attention of researchers has been attracted by the possibility of using various methods of non-drug exposure. Among these methods, various types of electropuncture (EP) are of considerable interest, based on exposure to biologically active points with alternating current.

Of the iron preparations currently used to treat animals with anemia and administered by mouth, iron glycerophosphate is the most effective (Alikayev V.A. Prevention and treatment of diseases of young farm animals. - Moscow: Kolos, 1968. - p. 252 )

The disadvantages of this method are:

- prolonged use of pharmacological agents;

- high time spent on diagnostics;

- the complexity of the treatment.

The objective of the proposed method is to increase the effectiveness of treatment, reducing the number of measurements, reducing the complexity and time spent on treatment, eliminating complex equipment.

The problem is solved due to the fact that in the known method of treating dogs with iron deficiency anemia, namely, that the treatment is carried out with a pharmacological preparation according to the invention, biopheron is used as a pharmacological preparation, which is administered to animals intramuscularly at a dose of 1 ml per 40 kg of body weight in within 5 days and additionally act on symmetric biologically active points (BAP) of the liver and spleen meridians Liv ₂ , Liv ₃ , Liv ₁₄ , SP ₆ , SP ₉ , SP _{10 with an} alternating current of 10-15 μA for a duration of 60 seconds for 5 days, the BAP conductivity is measured at positive and negative polarities.

Bioferon is a modern iron-dextran preparation made from high-quality raw materials from PHARMACOSOS A / S (Denmark). Contains 100 mg / ml (10%) of Fe ^{3+ irondextran} . Bioferon is a brown liquid in the form of a sterile solution. Composition (%): irondextran 10; phenol 0.5; water up to 100. The bioferon preparation has antianemic properties due to the presence of an iron-containing complex. The drug is rapidly absorbed and easily absorbed by the body, providing effective prevention and treatment of iron deficiency anemia.

As criteria for assessing the therapeutic effect, data are used to normalize hematological parameters and iron levels, changes in the value of bioelectric potential at negative and positive polarities at biologically active points of the liver and spleen meridians.

To clarify the essence of the proposed method, a drawing is presented, which shows the BAT of the meridians of the liver and spleen of a dog LIV ₂ , Liv ₃ , Liv ₁₄ , SP ₆ , SP ₉ , SP ₁₀ .

Using this treatment method leads to a change in the cellular composition of the peripheral blood of animals. The increase in phagocytic activity under the influence of electropuncture is provided primarily by spleen blood cells. Therefore, the meridian points of the liver and spleen were selected (Liv ₂ , Liv ₃ , Liv ₁₄ , SP ₆ , SP ₉ , SP ₁₀ , see drawing). The anemic condition was also indicated by unequal current conductivity when measuring the potentials in the BAT of these meridians.

When measuring potentials in the BAP at positive and negative polarities, the current conductivity is not the same if there are diseases or functional impairments. Such an asymmetry in the current conductivity, as well as the electrical resistance of the skin, is associated with the state of certain organs and systems.

When the value of positive polarity prevails, it is believed that the BAP is excited, if negative, it is inhibited.

To provide a therapeutic effect in the first case, it is necessary to influence the BAP with a positive, and in the second, negative potential. A positive potential creates a sedative, and a negative tonic effect.

The method is as follows.

The asymmetry of the BAT electrical conductivity was measured using the PERT-5 device.

In the process, it is necessary to monitor the presence of constant contact of the electrodes of the device with the body of the animal.

The prevalence of a negative polarity value in the BAP indicates their inhibition. The impact on the point with an alternating current of 10-15 μA for 60 seconds creates an ionizing effect in it, which is the basis for the activation of organs (liver, spleen). This significantly accelerates the redistribution of iron in the body and normalizes its content in the blood.

For treatment, 31 dogs of the German Shepherd breed of both sexes, aged from 1.5 to 8 years old, belonging to the Central Control Center under the Department of Internal Affairs of the Oryol Region, were used. The diagnosis of iron deficiency anemia was made as a result of a clinical examination of animals, blood tests for hematological parameters, wool for iron content, potential level at biologically active points of the liver and spleen meridians.

The statistical significance of the results was verified using biometric methods.

The BAP potentials of the meridians of the liver and spleen of animals before and after treatment were measured symmetrically using the PERT-5 instrument in the “search” mode. The readings were taken from a microammeter with a division value of 5 μA. The multiplicity of measuring the potential at each point and in each animal is 3, with an interval of 10 minutes. The average value of the potential was calculated (Table 2).

Simultaneously with the appointment of electro-puncture for animals, the iron-dextran preparation Bioferon was used.

For animals, the drug was administered intramuscularly at a dose of 1 ml per 40 kg of body weight for 5 days.

Example 1 (biopheron).

Bitch, German shepherd, aged 5 years old, belonging to the kennel of the Military District Directorate at the Department of Internal Affairs of the Oryol Region Iron deficiency anemia of moderate severity was diagnosed as a result of significant bleeding during pathologically childbirth. Hematological parameters, as well as the level of iron in the coat and the potential BAP of the meridians of the liver and spleen are reduced by an average of 12-73%. So, the hemoglobin level was 7.0 g / l; the number of red blood cells 4.5 × 10 ¹² / l; erythrocyte diameter 5.0 μm; CPU 0.7; hematocrit 28.5%; the level of reticulocytes is 6.6%; the iron content in the blood serum is 75 mcg / 100 ml. The conductivity index on the positive polarity corresponded to 25.3 μA, on the negative 32.2 μA at the point Liv ₂ ; 21.8 and 33.5, respectively, at the point of Liv ₃ ; 21.3 and 27.1 at point Liv ₁₄ ; 23.9 and 32.7 at point SP ₆ ; 18.0 and 31.2 at point SP ₉ ; 21.8 and 34.5 at point SP ₁₀ . The treatment was carried out as follows: the animal bioferon was administered intramuscularly at a dose of 1 ml per 40 kg of body weight for 5 days. Hematological parameters returned to normal on the 15-17th day from the start of treatment and amounted to: hemoglobin level 11.0 g / l; the number of red blood cells is 5.3 × 10 ¹² / l; erythrocyte diameter 5.2 microns; CPU 0.9; hematocrit 38.5%; reticulocyte level 1.6%; the iron content in the blood serum is 99.5 μg / 100 ml.

Example 2 (biopheron + electropuncture).

Bitch, German shepherd, aged 3 years old, belonging to the center of the cynological service at the Department of Internal Affairs of the Oryol region. As a result of chronically occurring gastroenteritis, iron deficiency anemia of moderate severity was diagnosed. When conducting blood tests, hair coat, values of potentials of biological active points of the liver and spleen meridians, a significant decrease in the studied parameters was noted by an average of 15-62%. So, the hemoglobin level was 7.4 g / l; the number of red blood cells is 4.6 × 10 ¹² / l; erythrocyte diameter 5.2 microns; CPU 0.7; hematocrit 29.3%; reticulocyte level 6.5%; the iron content in serum is 76 mcg / 100 ml. In this animal, the conductivity index on the positive polarity corresponded to 24.2 μA, on the negative 31.7 μA at the point Liv ₂ ; 20.8 and 31.7, respectively, at the point of Liv ₃ ; 21.7 and 26.7 at Liv ₁₄ ; 24.2 and 32.5 at point SP ₆ ; 17.5 and 30.0 at point SP ₉ ; 22.5 and 35.0 at point SP ₁₀ . The treatment was carried out as follows: the animal bioferon was administered intramuscularly at a dose of 1 ml per 40 kg of body weight for 5 days with the simultaneous use of electropuncture BAT meridians of the liver and spleen for 5 days. After treatment, the hematological parameters returned to the 5th day from the start of treatment and amounted to: hemoglobin level 11.2 g / l; the number of red blood cells is 5.4 × 10 ¹² / l; erythrocyte diameter 5.2 microns; CPU 0.9; hematocrit 37.5%; reticulocyte level of 1.5%; the iron content in the blood serum is 99.3 mcg / 100 ml. The conductivity at the positive polarity corresponded to 31.2 μA, at negative 31.7 μA at the point Liv ₂ ; 30.8 and 30.7, respectively, at point Liv ₃ ; 25.7 and 26.7 at Liv ₁₄ ; 32.2 and 32.5 at point SP ₆ ; 30.5 and 30.0 at point SP ₉ ; 34.5 and 35.0 at point SP ₁₀ .

Based on the evaluation of the effectiveness of treatment of animals with iron deficiency anemia, the condition of the dogs was monitored by a 4-fold (5, 10, 15, 20 days after the start of treatment) blood test for the main indicators (hemoglobin level, erythrocyte content and their diameter, color calculation indicator, the content of serum iron and iron in the coat) (table 1)

After treatment of the animal using biopheron, the studied blood indices and BAP potential values of the liver and spleen meridians returned to normal on the 10-15th day from the start of treatment.

After treatment using electropuncture in combination with biopheron, the studied hematological parameters corresponded to the norm on the 5-7th day from the start of treatment, the potential values in the BAT of the liver and spleen meridians returned to normal on the 5-7th day from the start of the treatment and corresponded to 30.2 μA on average at positive and negative polarities.

Removing the inhibition of the points of the meridians of the liver and spleen is proved by aligning the readings of the microammeter in the positive and negative polarities after 5 sessions of electro-puncture.

The use of electropuncture reduced the normalization time of hematological parameters and iron by an average of 5-7 days (Table 2).

Using the proposed method of treatment leads to a change in the cellular composition of the peripheral blood of animals. The increase in phagocytic activity under the influence of electropuncture is provided primarily by spleen blood cells. Therefore, the selected points of the meridians of the liver and spleen. Analysis of the data processed biometrically allows us to talk about the statistical reliability of the results, which indicates the effectiveness of the proposed method for the treatment of animals with iron deficiency anemia.

Table 1
The main hematological parameters before and after treatment of dogs with iron deficiency anemia Dog groups (treatment methods) Study time Indicators Hemoglobin g / l Red blood cells, 10 ¹² / l The diameter of the erythrocytes., Microns Color indicator Hematocrit% Reticulocytes,% Iron, mcg / 100 ml. Biopheron one 7.1 ± 0.2 4.6 ± 0.1 5.1 ± 0.1 0.7 ± 0.01 28.6 ± 0.1 6.5 ± 0.2 76.0 ± 1.0 2 8.8 ± 0.2 4.8 ± 0.1 5.2 ± 0.1 0.8 ± 0.01 30.5 ± 0.2 4.6 ± 0.2 82.8 ± 0.9 3 10.0 ± 0.1 5.0 ± 0.2 5.2 ± 0.2 0.9 ± 0.02 35.0 ± 0.1 2.9 ± 0.1 90.6 ± 1.0 four 10.8 ± 0.1 5.3 ± 0.1 5.2 ± 0.1 0.9 ± 0.02 38.2 ± 0.1 1.6 ± 0.2 98.7 ± 0.9 5 12.5 ± 0.1 6.7 ± 0.2 6.6 ± 0.1 0.9 ± 0.01 40.6 ± 0.1 1.3 ± 0.1 108.2 ± 0.9 Bioferon + EP one 7.5 ± 0.1 4.6 ± 0.2 5.2 ± 0.1 0.7 ± 0.01 29.5 ± 0.2 6.7 ± 0.2 76.3 ± 0.9 2 9.0 ± 0.1 4.9 ± 0.1 5.2 ± 0.1 0.8 ± 0.01 34.4 ± 0.2 4,5 ± 0,1 80.6 ± 1.0 3 11.2 ± 0.2 5.4 ± 0.1 5.2 ± 0.2 0.9 ± 0.01 37.2 ± 0.1 1.3 ± 0.2 98.4 ± 0.9 four 13.4 ± 0.1 6.0 ± 0.2 5.4 ± 0.2 0.9 ± 0.02 42.0 ± 0.2 1.0 ± 0.2 105.2 ± 0.8 5 14.0 ± 0.2 6.8 ± 0.1 6.0 ± 0.1 0.9 ± 0.01 45.6 ± 0.1 0.8 ± 0.1 109.7 ± 0.9 Note: 1 - before treatment; 2 - on the 5th; 3 - on the 10th; 4 - on the 15th; 5 - on the 20th day of treatment p <0.05

table 2
Dynamics of BAP potentials of liver and spleen meridians in dogs before and after treatment Dog groups (treatment methods) Study time Indicators Liver meridian Spleen meridian Liv ₂ Liv ₃ Liv ₁₄ SP ₆ SP ₉ SP ₁₀ (-) (+) (-) (+) (-) (+) (-) (+) (-) (+) (-) (+) Bioferon + EP 31.7 ± 1.1 24.2 ± 1.2 31.7 ± 1.2 20.8 ± 1.2 26.7 ± 1.1 21.7 ± 1.1 32.5 ± 1.2 24.2 ± 1.3 30.0 ± 1.1 17.5 ± 1.2 35.0 ± 1.3 22.5 ± 1.2 31.5 ± 1.0 27.4 ± 1.1 31.5 ± 1.2 28.0 ± 1.1 26.5 ± 1.0 26.0 ± 1.1 32.0 ± 1.1 34.2 ± 1.2 30.2 ± 1.0 26.2 ± 1.1 35.0 ± 1.3 30.2 ± 1.2 31.5 ± 1.1 31.0 ± 1.2 31.5 ± 1.1 31.0 ± 1.2 26.5 ± 1.0 26.4 ± 1.1 32.2 ± 1.2 34.0 ± 1.2 30.0 ± 1.1 30.2 ± 1.2 35.2 ± 1.2 35.0 ± 1.1 31.7 ± 1.1 31.4 ± 1.1 31.7 ± 1.2 31.5 ± 1.1 26.7 ± 1.1 26.7 ± 1.1 32.5 ± 1.1 34.0 ± 1.3 30.0 ± 1.0 30.2 ± 1.1 35.4 ± 1.2 35.2 ± 1.1 31.7 ± 1.0 31.5 ± 1.1 31.5 ± 1.2 31.5 ± 1.1 26.5 ± 1.1 26.5 ± 1.1 32.5 ± 1.1 34.2 ± 1.2 30.2 ± 1.2 30.2 ± 1.1 35.2 ± 1.3 35.2 ± 1.2 Note: 1 - before treatment; 2 - on the 5th; 3 - on the 10th; 4 - on the 15th; 5 - on the 20th day of treatment.

An analysis of these tables allows us to conclude that the most effective treatment regimen using electropuncture in combination with biopheron turned out to be the most effective.

Using the proposed method of treatment allows you to activate the activity of the blood-forming organs - the liver and spleen, significantly reduce the normalization of the studied parameters and accelerate the recovery of animals.

Claims (1)

A method of treating dogs with iron deficiency anemia, which consists in the fact that the treatment is carried out with a pharmacological preparation, characterized in that biopheron is used as a pharmacological preparation, which is administered intramuscularly at a dose of 1 ml per 40 kg of body weight for 5 days and additionally affects symmetrical biologically active points (BAP) of the meridians of the liver and spleen Liv ₂ , Liv ₃ , Liv ₁₄ , SP ₆ , SP ₉ , SP _{10 with an} alternating current of 10-15 μA, lasting 60 s, for 5 days, measure the conductivity of the BAP on positive and negative polarities, they are compared and, if the value of positive polarity prevails, then they act on the BAP with a positive potential, and if negative, they are affected by a negative potential, and the effect is carried out until there is no difference in the conductivity indices, after which the recovery of the animal is diagnosed.