RU2271740C2 - Method for studying fasciae and cellular spaces - Google Patents

Abstract

FIELD: medicine, experimental surgery.

SUBSTANCE: the present innovation deals with increasing the quality while studying fasciae and cellular spaces of anatomical objects upon unfixed cadavers. One should carry out tough injecting and preparing, moreover, connective-tissue neoplasms of the space should be stained with 0.1%-amido black solution 10 V to be introduced under the pressure of 50 mm mercury column.

EFFECT: higher efficiency.

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Description

The invention relates to medicine, namely to experimental surgery, and can be used in the study of fascia of cellular spaces.

The study of cellular spaces is of undoubted interest, both for clinical anatomists and for practical surgeons. Treatment of purulent-inflammatory diseases and hematomas is impossible without reliable information about their surgical anatomy. Data on the linear dimensions, boundaries, volume of cell spaces is extremely important for reconstructive surgery. From a biochemical point of view, cell spaces are relatively isolated large protein capsules loosely filled with fat filling the cells between the inner walls of the capsule. The ratio of protein and fat components varies significantly in individuals of different constitutional types, which is important in the dynamics of the development of a pathological process.

In the study of patent and scientific medical literature, the following methods were identified that are used for a similar purpose, namely, a method for studying fasciae and cellular spaces using a special Delitsin cannula [1], fascia and cellular spaces are densified with 10% zinc chloride solution before examination. The disadvantage of this method is the violation of the topographic anatomy of the cellular space during fixation, the inability to visualize connective tissue formations inside the space. The method does not provide for the study of structural-quantitative study of the relationship between the fat and protein components of the cellular space in the age, gender and constitutional aspects.

A known method of disk electrophoresis [2], which uses a 0.2% solution of black amido 10 V in 7% acetic acid. The disadvantage of this method is the impossibility of its use for the study of fascia and cellular spaces. In addition, a solution of this composition extremely intensively stains large connective tissue formations.

The prototype of the present invention is a method of filling cell spaces [3, 4], which uses gypsum, flour, talc, glycerin, barium sulfate (or lead) in various proportions, introduced into the cell space through a Dufo needle.

The disadvantages of the prototype are the inability to paint the walls of the cellular space, difficulties in documenting the materials obtained. Like analogues, the method does not provide for the study of the structural-quantitative relationship of the fat and protein components of the cellular space in the age, sex and constitutional aspects, it is necessary to use the x-ray technique to study cell space messages. The communication of spaces is determined by the rupture of the thin spots of the fascia by a mixture introduced under high pressure, which changes the architectonics of space.

The task of the invention is to improve the quality of research on cellular spaces on unfixed corpses and to document the results without additional material costs.

The problem is achieved in that the connective tissue formations of space for 20 minutes are stained with a 0.1% solution of black amido 10 V, which is injected under a pressure of 50 mm Hg

The method is as follows. A 0.1% solution of black amido black 10 V under a pressure of 50 mm Hg is injected through the Dufo needle into the cellular space. The volume of the injected solution is recorded. After a 20-minute exposure, according to the objectives of the experiment, the space is opened and washed first with 7% acetic acid and then with room temperature water, while the fatty tissue is washed from the dye and acquires a natural color, and connective tissue formations (fascia, trabeculae, fascial nodes, the edges of the holes in the fascia) are painted blue, while the direction of the collagen bundles is visualized. Color photographs are taken.

An example of a specific implementation of the method.

An unfixed corpse of a woman of a dolichomorphic physique was laid on her stomach. At 1 cm anterior to the tip of the coccyx, a Dufo needle was injected to a depth of 2.5 cm into the retrorectal space under a pressure of 50 mm Hg. injected 150 ml of a 10% solution of black amido 10 V. After 20 minutes, a tailbone was exposed by a longitudinal section of 15 cm, which was then mobilized and removed. The resulting cavity was washed first with 7% acetic acid and then with room temperature water, the fatty tissue was washed from the dye and turned natural, the connective tissue formations turned blue, and the direction of the collagen bundles and connective tissue septa that separated the cells of fatty tissue were visualized . It was found that the retrorectal space has the shape of a truncated pyramid located with its base up. The upper border of retrorectal space is projected onto the fifth sacral vertebra. The anterior wall of the retrorectal space is the visceral fascia of the rectum, the posterior wall is formed by the sacral fascia, with which the vaginas of the common and internal iliac vessels are fused, separating the retrorectal space from the mediastinum of the abdomen. The lower wall of the space is represented by the fascial case of the coccygeal muscle and the parietal sheet of the pelvic fascia, covering the muscle that raises the anus. Between the coccygeal and piriformis muscles under the pelvic fascia is the genital nerve, which goes to the external sphincter of the rectum. Between individual portions of the muscle that lifts the anus, 2 radial slots on the right and 1 on the left were revealed. The lateral walls of the space are represented by ligaments from the pubic bones to the sacrum. Projected color photographs were performed.

The method allows to study fascia and cellular spaces, macroscopically differentiating with the biochemical structure of education, and to clarify the message space.

Information sources

1. Shevkunenko V.N., Geselevich A.M. Typical human anatomy. - L.- M .: OGIZ, 1935. - 8 p.

2. Maurer F. Disc electrophoresis. - M., 1977 .-- 58-67 p.

3. Kovanov VV, Anikina T.I. Surgical anatomy of fascia and cellular spaces in humans. - M., 1961.

4. Khomenko N.F. On the methodology for studying fascia and cellular spaces of the forearm. // In the book: Some questions of the pathology of the digestive tract and retroperitoneal space. - Chita, 1970 .-- 178-179 p.

Claims (1)

A method for studying fasciae and cellular spaces of anatomical objects by tight injection and preparation, characterized in that the connective tissue formations of the space for 20 minutes are stained with a 0.1% solution of black amido 10 V, which is injected under a pressure of 50 mm Hg