RU2271173C1 - Method for cholesterol level reducing - Google Patents

Abstract

FIELD: applied biology, in particular methods for cholesterol level reducing in poultry (goose) body.

SUBSTANCE: claimed method includes administration of probiotic lactoamilovorine having colony forming units (CFU) approximately 0.243-4.26 x 10¹⁰ per 1 g. During the first life month probiotic is introduced into gosling ration in dose of 70 g per 1 ton of compound feed, wherein during the first three life days probiotic is additionally administered with water in dose of 7 g/10 l.

EFFECT: method with effective anticholesteronemic action; goose meat of improved nutritive value.

Description

The invention relates to applied biology, and in particular to methods for lowering cholesterol in the body of a bird (geese).

There is a method of reducing cholesterol in the blood serum of broiler chickens, which consists in the long-term oral administration of lactobacilli to the body of a bird with food [Jin LZ, But YW, Abdullah N., Jalaludin S. Growth performance, intestinal microbial populations, and serum cholesterol of broilers fed diets containing Lactobacillus cultures.// Poult. Sci. -1998. - No. 77 (9). - P.1259-1265].

The disadvantage of this method is that a decrease in the level of cholesterol in the blood serum of a bird is observed only during the period of constant introduction of lactobacilli into the body of a bird with food.

The technical result of the invention is the reduction of cholesterol in a living organism, in this case due to the short-term use of a probiotic.

The problem is solved in that lactoamilovorin with a colony forming unit titer (CFU) in the range of 0.243-4.26 × 10 ¹⁰ per 1 g is used as a probiotic, and during the first month of life, goslings are introduced in a dose of 70 g / t of feed, and in the first three days, 7 g of the drug is additionally given with water per 10 liters.

To confirm the effectiveness of the proposed method, groups were formed by the method of pair analogues. Goslings were grown from daily to 30 days of age indoors, and then on pasture. During the experiment, the zootechnical parameters recommended by the poultry factory specialists were observed. The bird had constant free access to feed and water. In the course of the experiment, planned veterinary measures were also carried out. Consequently, the conditions of detention and the general feeding level were the same for goslings of all groups, the differences consisted only in the fact that experimental goslings were additionally fed lactoamilovorin in the above doses during the first month of life. In the experiment we used a probiotic with a titer of colony of forming units (CFU) in the range of 0.243-4.26 × 10 ¹⁰ in 1 g, which was prepared in the laboratory of biotechnology of microorganisms VNIIIFBiP s.kh. animals.

In all experiments, geese received mixed feed, the composition of which corresponded to the standards of VNIITIP. Before feeding this compound feed, lactoamilovorin was added to it at a dose of 70 g / t. Mixing was carried out in a vibratory mixer (patent No. 2201796). Weights were carried out every day on a Kenwood electric balance. Preservation was taken into account daily by the number of dead birds. Carcasses were cut according to the methodology of VNIITIP (2001). Blood was taken from the wing vein (up to one month of age total) from 5 individuals from each group, and heparin was used as an anticoagulant. To determine the assimilation of food nutrients in geese using lactoamilovorin, physiological (balance) experiments were carried out at the ages of 30 and 60 days according to the method of VNIITIP (2001).

Total cholesterol was determined in the blood (Kolb V.G., Kamyshnikov B.C., 1976). Fat was determined in feed by the Rushkovsky method (Maslieva O.V., 1970), in the litter it was determined by the same method as in feed. The amount of fat in meat was determined by the generally accepted method (Maslieva O.V., 1970), and the content of total cholesterol according to the Zack method described by V. Martyushov. et al. (1997).

The digital data obtained during the experiment were processed statistically (Gataulin AM, 1992), the significance of differences between the groups was calculated by the Student method described by the above author. The differences were considered significant at P≤0.05.

Of particular interest are the features of age-related dynamics of the total cholesterol content in the blood serum of geese of the experimental and control groups. The results of studies of the content of total cholesterol in the blood serum of geese of both groups are presented in table 1.

Table 1
The content of total cholesterol in the blood serum of geese, g / l Age (days) Group Control Experienced one 3.452 ± 0.056 10 2.135 ± 0.021 2,472 ± 0,097 * twenty 1.975 ± 0.059 1,754 ± 0,097 * thirty 1,756 ± 0,072 1.358 ± 0.095 * 40 1.523 ± 0.089 1.148 ± 0.144 * 120 1.828 ± 0.075 1,449 ± 0,083 * 180 1.713 ± 0.043 1.321 ± 0.039 *

Analysis of the research results showed the presence of a high concentration of total cholesterol in the blood serum of geese on the first day after hatching - 3.452 ± 0.056 g / l. By 10 days of age, a decrease in the value of this indicator by 61.69% in the control and 39.64% in the experimental group was noted, amounting to 2.135 ± 0.021 and 2.472 ± 0.097 g / l, respectively. A decrease in serum cholesterol was observed in the goslings of the experimental and control groups for 20, 30 and 40 days of age, however, the decrease in the concentration of total cholesterol was more intense in the goslings of the experimental group, and at the age of 40 days the level of total cholesterol reached its minimum values for the entire study period of ontogenesis, amounting to 1.523 ± 0.089 g / l in the control group and 1.148 ± 0.144 g / l in the experimental group. By 120 days of age, the level of total cholesterol rises in the control group to the level of 1.828 ± 0.075 g / l and in the experimental group to 1.499 ± 0.083 g / l and slightly decreases by the age of 180 days, when the concentration of total cholesterol in the blood serum of geese of the control group is 1.713 ± 0.043 g / l and of the experimental group 1.321 ± 0.039 g / l. It should be noted that the concentration of total cholesterol in the blood serum of geese of the experimental group at 10 days of age was statistically significantly higher by 15.78% than in the control group, and subsequently statistically significantly lower, and the difference was 12.6 at the age of 20 days %, 30 - 29.31%, 40 - 32.67%, 120 - 26.16% and 180 - 29.67% in favor of the bird of the control group.

At 180 days of age, control slaughter of the bird, anatomical cutting and determination of the chemical composition of poultry meat of both groups were carried out. The use of lactoamilovorin had a definite effect on the chemical composition of the muscles of geese. The analysis data are presented in table 2. Thus, the meat of geese of the control group in its composition contained more water by 3.35% and fat by 3.0% than the meat of geese of the experimental group. However, the protein content in goose meat was 5.72% higher, and the difference in mineral content was statistically unreliable. It should be noted that the cholesterol content in the poultry meat of the experimental group was statistically significantly lower by 49.59%, which from the point of view of dietetics makes meat especially valuable.

The content of nitrogen-free extractive substances in poultry meat of the control group was slightly lower. The amount of tryptophan in the meat of geese of the experimental group was statistically significantly higher by 4.58%, and the difference in the content of oxyproline was statistically unreliable, however, the protein-quality indicator of poultry meat of the experimental group was higher by 12.25%.

table 2
The chemical composition of goose meat Indicator Group Control Experienced Protein,% 15.49 ± 0.41 21.21 ± 0.37 * Fat% 11.81 ± 0.15 8.81 ± 0.13 * Water% 70.38 ± 2.15 67.03 ± 1.76 * BEV,% 1.44 2.01 Ash% 0.88 ± 0.07 0.93 ± 0.04 Tryptophan, mg / kg 4055.2 ± 87.1 4241.0 ± 81.4 Oxyproline, mg / kg 477.5 ± 27.9 445.2 ± 31.7 Protein-quality indicator 8.49 9.53 Cholesterol, mg / kg 694.1 ± 6.7 464.0 ± 2.8 *

At 30 and 60 days of age, physiological studies were carried out, which showed that at the age of 30 days the goslings of the experimental group were 41, 68% less than the control group, digested feed lipids, and at 60 days, 44.5%.

The most likely reason for the reduction in the absorption of food lipids in experimental geese is the property of various species of lactobacilli living in the digestive tract, described by Gilliland S.E. (1990), deconjugate bile acids such as taurocholic and glycocholic, and since deconjugated bile acids provide less absorption of lipids from the intestinal tract, therefore, a certain amount of feed lipids passes through the gastrointestinal tract in transit and, accordingly, is not absorbed into the blood. The basis for lowering the level of cholesterol in the blood serum of geese of the experimental group may also be the ability of lactoamilovorin to deconjugate bile acids, and since cholesterol is a lipid, it is also less absorbed from the intestine, and therefore its level in the blood decreases.

However, this is not the only probable reason for lowering blood cholesterol. So, Gilliland S.E. and others (1985) found that some types of lactobacilli are able to assimilate cholesterol in the presence of bile and under anaerobic conditions from laboratory media. Apparently, the strain of Lactobacillus amylovorus BT 24/88 has two of the above properties, and in this case, probably, there is a phenomenon called synergism, when the combined effect of two or more factors is greater than the estimated sum of the effects of each of the factors. This assumption is not accidental, since I. Smorodintsev (1952) reports that when white mice were fed with cholesterol-free food, after the expiration of the test period, 0.242 g of cholesterol was found in them, while in the controls it was 0.158 g, and similar experiments in rats showed that at the end of the experiment, the animals did not weigh differed from control rats, and the cholesterol content increased by 124 mg relative to the beginning of the experiment. The same author reports that the effect of food cholesterol on blood cholesterol is different in omnivores and herbivores. So, in omnivores a few hours after ingestion of food rich in cholesterol, there is a slight, rapidly passing increase in blood cholesterol, and prolonged feeding with this food does not enrich their blood and organs with cholesterol, but in herbivores, which include geese, on the contrary, an increase in cholesterol in blood is detected not after a single meal, but after 2-3 days. It should be noted that our studies of the age-related dynamics of the content of total cholesterol in blood serum practically coincide with the data of G. Alenichkina. (1971) obtained on ducks, and the fact established by the author that the decrease in blood cholesterol does not adversely affect the growth, development and weight gain of the bird coincides.

Thus, compared with the prototype of the claimed method has a more pronounced and effective anticholesterolemic effect, which makes the meat of geese more valuable for eating.

Claims (1)

A method of reducing the cholesterol level in the body of geese, including the use of probiotics, characterized in that lactoamilovorin with a titer of colony forming units (CFU) in the range of 0.243-4.26 × 10 ¹⁰ per 1 g is used as a probiotic, and during the first month of life in the diet goslings are administered in a dose of 70 g / t of feed, and in the first three days, 7 g of the drug is additionally given with water per 10 l.