RU2263312C1 - Method for detecting animals suffering with cattle leukosis at aleukemic and leukopenic manifestations of the disease - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: the present innovation deals with detecting RID-positively reacting animals to establish the quantity of leukocytes in blood, for this purpose one should additionally perform differentiated counting for leukocytes in animals with normal quantity of leukocytes and leukopenia and in case of lymphocytes' percentage in blood smears being above the norm corresponding to 65-70% and more this animals should be considered as a sick one. The suggested innovation enables to conduct more accurate and reliable diagnostics of leucosis in due time.

EFFECT: higher accuracy and efficiency of detection.

2 ex, 2 tbl

Description

The invention relates to veterinary medicine and can be used to identify patients with cattle leukemia in animals with aleukemic and leukopenic manifestations of the disease.

Known hematological method for the detection of a patient with cattle leukemia of an animal, including the preliminary identification of animals that respond positively to RID, based on the detection of specific precipitating antibodies to cattle leukemia virus antigens in blood serum. Animals whose blood serum tested positive in RID are recognized as infected with the leukemia virus and examined by the hematological method, which consists in counting white blood cells per unit volume of blood. If an increased number of them is detected, the leukocyte formula is derived and the status of the animal is assessed by leukemia according to the “leukemia key” as healthy, suspicious for leukemia or suffering from leukemia. Sick animals are removed from the herd [1].

The disadvantage of this method is that animals that respond positively to RID, but have a normal or low white blood cell count and evaluated as “healthy” according to the instructions, do not undergo further hematological studies, and therefore cases with aleukemic and leukopenic manifestations cannot be detected illnesses. Undetected patients with leukemia animals that do not have expressed hematological parameters characteristic of leukemia (leukocytosis, absolute lymphocytosis) continue to be in the herd and contribute to the development and acceleration of the infectious and epizootic processes of the disease.

The task of the invention is to improve the quality, reliability and accuracy of diagnosis, timely (early) identification of sick animals with hematologically unexpressed leukemia (aleukemic and leukopenic manifestations of the disease) for their subsequent rejection.

EFFECT: timely detection of animals with leukemia of animals with aleukemic and leukopenic manifestations of the disease for their culling.

The specified technical result is achieved by first identifying animals that are positively responsive to RID, leukocytes are counted per unit volume of blood, then animals with a normal (aleukemic) leukocyte count and leukopenia additionally carry out differentiated leukocyte counting. Assess the animal as a patient in the presence of a relative content of lymphocytes above normal. Such an animal is discarded.

Example 1

In the experiment, there were 297 heads of cattle of 3-6 years of age, of which 85 goals were found that have a positive reaction in RID and 212 were seronegative in RID with respect to cattle leukemia virus antigens. All animals had a quantitative and qualitative leukocyte count.

From table 1 it follows that of the 85 goals of RID - positive animals, all found a different number of leukocytes and the percentage of lymphocytes. In this regard, RID (+) animals are divided into 4 groups:

1 group. This includes 57 heads of cattle with a normal number of leukocytes in 1 μl of blood (6.71 ± 0.24), normal percentage of lymphocytes (61.40 ± 1.44), which are referred to healthy animals by the “leukemic key”.

2 and 3 groups. This includes 10 heads of cattle with leukocytosis, for which further hematological studies are carried out according to the “Guidelines for the diagnosis of cattle leukemia” (2000).

4 group. This includes 8 heads of cattle with a normal number of leukocytes in 1 μl of blood (8.09 ± 0.64 thousand), in which the absolute number of lymphocytes is within normal limits and is 6.11 ± 0.63 thousand / μl ( table 1), i.e. in this case, the hematological parameters (the number of leukocytes, the absolute number of lymphocytes) for the “leukemia key” are within the parameters of healthy animals, except for the percentage of lymphocytes, which in this case is not determined according to the “Guidelines ...” and accordingly is not taken into account when making a diagnosis for leukemia [1, p. 14]. As follows from table 1, in animals of this group, a significantly high percentage of lymphocytes (75.50 ± 2.38) is detected (P <0.001), which exceeds that in healthy animals and indicates the development of an infectious process in cattle leukemia despite " calm "indicators of the leukemic key."

The status of animals with a differential count of leukocytes of 65-70 or more percent of lymphocytes, taking into account age, is evaluated as patients with leukemia and rejected.

Example 2

We studied 142 heads of cattle of 3-6 years of age, of which 114 goals were found that have a positive reaction in RID, and 28 goals of seronegative in RID in relation to cattle leukemia virus antigens. All animals had a quantitative and qualitative leukocyte count.

From table 2 it follows that in 114 goals of RID - positive animals found a different number of leukocytes and the percentage of lymphocytes. In this regard, RID (+) animals are divided into 3 groups:

1 group. This included 74 heads of cattle with a normal number of leukocytes in 1 μl of blood (7.87 ± 0.19) and a normal percentage of lymphocytes (61.86 ± 1.27), which are referred to healthy animals by the “leukemic key”.

2 group. This included 35 cattle with leukocytosis, for which further hematological studies are carried out according to the “Guidelines for the diagnosis of cattle leukemia” (2000).

3rd group. This included 5 heads of cattle with leukopenia (3.53 ± 0.47 thousand leukocytes in 1 μl of blood) and lymphopenia (2.76 ± 0.42 thousand lymphocytes in 1 μl of blood). As follows from table 2, in animals of this group, a reliably high (P <0.001) percentage of lymphocytes (77.80 ± 3.30) is detected, exceeding the norm for healthy animals and indicating the development of the leukemia process against the background of leukemia and lymphopenia.

The status of animals with a differential count of leukocytes of 65-70 or more percent of lymphocytes, taking into account age, is evaluated as patients with leukemia and rejected.

Thus, in order to identify patients with cattle leukemia in animals with aleukemic and leukopenic manifestations of the disease, animals that respond positively to RID are detected, the number of leukocytes in their blood is determined, and then an additional differential count of leukocytes is carried out in animals with a normal number of leukocytes and leukopenia, and the animal assessed as a patient with the presence in the blood smears of a percentage of lymphocytes above the norm of healthy individuals with respect to leukemia.

The proposed method allows for a more accurate and reliable lifetime diagnosis of leukemia.

Sources of information

1. Guidelines for the diagnosis of leukemia in cattle: Approved by the Department of Veterinary Medicine of the village. households of Russia: 08.23.00. - M., 2000. - S.12-16.

Table 1
Hematological indicators of cattle 3-6 years of age, seropositive to VLCKR, against a background of normal leukocyte count in 1 μl of blood The ratio of animals to VLCRS in RID Hematologically investigated, n The number of leukocytes, thousand / μl Lymphocytes,% The absolute number of lymphocytes, thousand / μl RID (-) n = 212 212 7.85 ± 0.26 62.70 ± 1.67 4.92 ± 0.43 READ (+)
n = 85 57 6.71 ± 0.24 61.40 ± 1.44 4.12 ± 0.16 10 19.15 ± 1.94 87.20 ± 1.62 16.70 ± 1.90 10 14.90 ± 1.01 63.10 ± 3.65 9.40 ± 0.74 8 8.09 ± 0.64 (up to 10-11 *) 75.50 ± 2.38 (up to 65-70 *) 6.11 ± 0.63 (up to 9-10 *) Total: 297 297

table 2
Hematological indicators of cattle 3-6 years of age, seropositive to VLCRS, with leukopenia The ratio of animals to VLCRS in RID Hematologically investigated, goal. The number of leukocytes, thousand / μl Lymphocytes,% The absolute number of lymphocytes, thousand / μl RID (-) n = 28 28 7.65 ± 0.36 55.90 ± 2.68 4.20 ± 0.26 RID (+) n = 114 74 7.87 ± 0.19 61.86 ± 1.27 4.88 ± 0.16 35 20.93 ± 2.74 73.54 ± 2.33 15.50 ± 2.05 5 3.53 ± 0.47 (up to 10-11 *) 77.80 ± 3.30 (up to 65-70 *) 2.76 ± 0.42 (up to 9-10 *) Total: 142 142 Note to tables 1 and 2:
* - indicators corresponding to indicators of healthy animals according to the "leukemic key" taking into account age

Claims (1)

A method for identifying patients with leukemia of cattle in animals with aleukemic and leukopenic manifestations of the disease, comprising identifying animals that are positively responsive to RID and determining the number of leukocytes in the blood, characterized in that they additionally carry out differentiated leukocyte counts in animals with normal leukocyte count and leukopenia and in the presence of percentage the lymphocyte content is above the norm of 65-70% or more, the animal is assessed as sick.