RU2251840C2 - Method for producing of acaricide-insecticide composition - Google Patents

Abstract

FIELD: agriculture, in particular, protection of plants from hazardous insects and mites.

SUBSTANCE: method involves using antidesiccant such as preparation produced from Mortierella nantahalensis micromycet biomass by predetermined process.

EFFECT: reduced consumption of nematode due to increased survivability thereof in composition.

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Description

The invention relates to a technology for the production of plant protection products from harmful insects and ticks.

A known method for the production of acaricidal-insecticidal compositions, comprising mixing a suspension of nematodes Steinernema caprocapsae and antidexicant in the form of fractions of vegetable oils (Pushnya M.V., Technology for the production of entomopathogenic microsporidia and nematodes and their use for protecting nightshade crops. Abstract of thesis. . - Krasnodar: VNIIBZR, 2000, p.14).

In such compositions, the nematodes die quickly enough, which leads to an increase in the consumption of nematodes to achieve their effective concentration.

The technical result of the invention is to reduce the consumption of nematodes by increasing their survival in the composition.

This result is achieved in that in a method for the production of an acaricidal-insecticidal composition, comprising mixing a suspension of nematodes of the genus Steinernema and an antidesicant. according to the invention, a preparation obtained by sequential extraction of biomass of micromycete Mortierella nantahalensis with a non-polar supercritical extractant, water, alkali, water, acid, water, alkali and water, followed by combining the first extract with a solid residue in an amount of 0.2- 1% by weight of the composition.

The method is implemented as follows.

The dry biomass of the micromycete Mortierella nantahalensis is extracted with a non-polar extractant, for example carbon dioxide or hexane, in a supercritical state. At this stage, the first extract is separated, which is used later in the preparation. Next, the biomass is sequentially extracted with water, alkali, water, acid, water, alkali and water. The solid residue obtained after completion of all the extraction steps listed above is combined with the first extract. The resulting preparation contains mainly chitosan and higher polyunsaturated fatty acids and does not contain phytotoxic substances, which allows it to be used as part of pesticides. The drug is injected into a suspension of entomopathogenic nematodes of the genus Steinernema in an amount of 0.2-1% by weight. The dosage of the preparation from the biomass of micromycete Mortierella nantahalensis is justified by the fact that, with its smaller amounts, the technical result is not achieved due to the loss of the antidisactic properties in such concentrations of the drug, and when its dosage is more than 1% to 1.5% by weight, the composition sharply increases the viscosity and begins gelation, which makes it difficult to use for its intended purpose.

Since the effectiveness of the use of entomopathogenic nematode compositions for plant protection is determined by the concentration of the latter in a viable state, the survival of the nematodes was determined immediately after the antidexicant was added using the proposed method and the closest analogue, as well as 1, 3, and 5 days after the antidexicant was added, i.e. the course of time when viable entomopathogenic nematodes are able to kill pests. In the experiments, nematodes of the species Steinernema caprocapsae and Steinernema feltiae were used. The experimental results for both types of nematodes coincided up to the experimental error. The experimental results are averaged for two types of nematodes and are summarized in table 1.

Table 1. Vedas antidexicant survival of nematodes,%; after exposure, days 0 1 3 5 by analogy 60,4 59.4 56.6 51.1 by the proposed method, 0.2% by weight 94.1 88.7 86.4 82.1 by the proposed method, 1% by weight 91.9 87.6 86.9 85.3 control without anti-desiccant 100 93.3 21.3 0

As can be seen from the table, the dynamics of the survival of nematodes in the composition according to the proposed method confirms the possibility of reducing their consumption compared to the closest analogue due to the possibility of obtaining compositions with the same activity while reducing the number of nematodes initially introduced into the composition.

Claims (1)

A method for the production of an acaricidal-insecticidal composition, comprising mixing a suspension of nematodes of the genus Steinernema and an anti-desiccant, characterized in that the preparation obtained by sequential extraction of the biomass of micromycete Mortierella nantahalensis with a non-polar supercritical state, water, alkali, water, alkali, water is used as an antidexicant alkali and water, followed by combining the first extract with a solid residue in an amount of 0.2-1% by weight of the composition.