RU2249381C2 - Mixed fodder premix preparation method - Google Patents

Abstract

FIELD: feed production.

SUBSTANCE: premix is prepared by adding special-type agents to divided grain filler according to special technology, said agents being based on biomass of micromycet of Mortierella sepedonioides species.

EFFECT: increased storage stability of premix.

2 dwg

Description

The invention relates to the feed industry.

A known method of producing feed premix, which involves grinding a filler containing grain or products of its processing and introducing into the filler the target additives in the form of biologically active substances and an antioxidant, the composition of which uses a lipid extract from biomass micromycete Mortierella minutissima (RU 2141226 C1, 11/20/1999) .

The disadvantage of this method is to obtain the target product having insufficiently high storage stability.

The technical result of the invention is to increase the resistance of the target product to storage.

This result is achieved by the fact that in the method of producing a feed premix involving grinding the filler containing grain or products of its processing and introducing target additives into the filler, the composition of which uses the preparation from biomass of micromycetes of the genus Mortierella, according to the invention from micromycetes of the genus Mortierella, the species Mortierella sepedonioides is used , and the drug is obtained by sequential extraction of biomass micromycete Mortierella sepedonioides non-polar extractant in a supercritical state, water, alkali, water, islotoy, water, alkali and water and then combining the first extract with the solid residue.

The method is implemented as follows.

The dry biomass of micromycete Mortierella sepedonioides is extracted with a non-polar extractant, for example carbon dioxide or hexane, in a supercritical state. At this stage, the first extract is separated, which is used later in the preparation. Next, the biomass is sequentially extracted with water, alkali, water, acid, water, alkali and water. The solid residue obtained after completion of all the extraction steps listed above is combined with the first extract. Thus obtained drug is introduced into the crushed grain filler together with other target additives, in particular with biologically active substances and, if necessary, an antioxidant.

The composition of the biomass micromycete Mortierella sepedonioides consists mainly of higher polyunsaturated fatty acids with vitamin and antioxidant activity, as well as chitosan. Fatty acids contain significant amounts of arachidonic and eicosapentaenoic acids, which, in combination with chitosan, have especially high immunostimulating activity on grain. The composition of the drug does not include anti-nutritional, mutagenic, carcinogenic and toxic substances, which makes it possible to use it as part of compound feeds, and also does not include substances that can inhibit the immunostimulating activity of a mixture of chitosan with arachidonic and eicosapentaenoic acids, which increases the stability of the resulting premix during storage.

It should be noted that grain treatment with the preparation from the biomass of micromycete Mortierella sepedonioides, obtained by the technology described above, is effective at a drug consumption starting from 0.5 mg / t. At the same time, the amount of antioxidant in the premix should be about 0.1%. That is, as part of the premix, the drug from the biomass of micromycete Mortierella sepedonioides can be used as a biologically active additive and antioxidant both independently and in combination with other components.

Example 1

The premix is prepared on the basis of corn grain, in which, as part of other components, a lipid extract from the biomass of micromycete Mortierella minutissima or a preparation from biomass of micromycete Mortierella sepedonioides, obtained according to the above technology using carbon dioxide as non-polar extractant, as alkali sodium hydroxide and as salt alkali are added acid, in an amount of 1 kg / g. After 1 month of storage, the premix samples of the reference and experimental batches are contaminated with Penicillium funiculosum mold spores in an amount of 2 × 10 ¹² spores / g and incubated at 36 ° C for 10 days. Qualitatively, on the samples of the reference premix, mold growth was detected; on the samples of the experimental premix, it was not detected.

Example 2

The premix is prepared using rice flour and crushed oat grains as a filler, into which, as an antioxidant, a mixture of butyloxytoluene with a lipid extract from the biomass of micromycete Mortierella minutissima or a preparation from biomass of micromycete Mortierella sepedonioides obtained using nonpolar extract as a non-polar is used ammonium hydroxide and propionic acid, at a flow rate of the last 1 mg / t. Check for mushroom resistance is carried out according to example 1, but using Aspergillus niger mold spores. The result coincides with example 1.

Thus, the proposed method allows to obtain the target product with higher stability during storage.

Claims (1)

A method of producing a premix for compound feed, which involves grinding a filler containing grain or products of its processing and introducing target additives into the filler, which use a preparation from biomass of micromycetes of the genus Mortierella, characterized in that from the micromycetes of the genus Mortierella use the species Mortierella sepedonioides, and the preparation is obtained by sequential extraction of the biomass of micromycete Mortierella sepedonioides with a non-polar supercritical extractant, water, alkali, water, acid, water, alkali and water, followed by conductive combining the first extract with the solid residue.