RU2246953C1 - Disinfecting agent - Google Patents

Abstract

FIELD: sanitary and hygienic materials.

SUBSTANCE: water-based disinfecting composition contains water-soluble cyclodextrin-iodine inclusion complex and/or derivatives thereof, iodine, iodide ion source, organic solvent, and, if necessary, surfactant and/or functional additive, all in specified proportions. Agent possesses wide spectrum of antimicrobial effects at low concentrations. It does not irritates skin, shows no toxicity, and is stable upon storage within a wide temperature range.

EFFECT: increased choice of high-efficiency disinfecting agents.

19 cl, 2 tbl

Description

The invention relates to disinfectants containing water-soluble complexes of iodine with organic compounds and having a wide spectrum of antimicrobial action, which allows them to be used to treat the hands of surgeons and medical staff, for hygienic disinfection in medical institutions, in kindergartens and schools, in social institutions provision, at communal facilities, catering, food industry, food trade, in the agricultural sector stve, at home with bacterial infections (including tuberculosis), viral and fungal etiology.

The use of iodine as a disinfectant has been known for a long time, and still alcoholic solutions of iodine are the most common antiseptics widely used to disinfect wounds and surgical fields. This is due to the wide range of antimicrobial activity of iodine.

The spectrum of antimicrobial activity of antiseptics and disinfectants. [J. Flewette, J. Freney, M.-E. Reverdy "Antisepsie et Desinfection", Editions ESKA, 1995]

As can be seen from the table, iodine is highly active in relation to gram-positive bacteria and average activity in relation to all other microorganisms. Only chlorine and aldehydes have the same activity, the spectrum of activity of the remaining biocides is much narrower.

A significant disadvantage of iodine when used in its pure form is a strong burning effect in relation to body tissues. Injuring the skin, iodine prevents the healing of wounds and postoperative sutures.

The development of polymer compositions of iodine with water-soluble polyvinylpyrrolidone (PVP) has eliminated this drawback. In this case, iodine is in a bound state due to the formation of a complex of ion I $\genfrac{}{}{0ex}{}{\text{-}}{\text{3}}$ with the C = O group of the amide bond of the pyrrolidone ring.

Known biocidal composition [Patent EP 0365565 B1, class. A 01 N 59/12, 1988], which is an aqueous solution of a complex of polyvinylpyrrolidone with iodine, stabilized with potassium iodide, the content of the latter being from 10 to 60% by weight of the complex itself. The disadvantage of this composition is its lack of stability in time and when heated.

Also known biocidal composition [Patent EP 0793418 B1, class. A 01 N 59/12, 1995], which is an aqueous solution of a complex of polyvinylpyrrolidone with iodine, stabilized with a nonionic surfactant, namely a block copolymer of ethylene oxide and propylene oxide, which has a low PVP: I ₂ ratio and is stable in a wide range of temperatures.

However, during storage, iodine gradually interacts with PVP, forming covalent bonds; therefore, compositions including complexes of polyvinylpyrrolidone with iodine lose their antimicrobial activity over time.

The closest in technical essence is the known iodine-containing biocidal composition, which is a concentrate of the complex of iodine with alkylpolyglucosides [Application PCT / US96 / 06975, cl. A 01 N 59/12, 1996].

The composition of this complex includes:

- 0.5-30% iodine;

- 0.2-14% of iodide in the form of a salt, acid or a mixture thereof;

- 2-85% nonionic surfactants, which is an alkylpolyglucoside of the general formula R ₁ OCR ₂ O) _b (Z) _a ,

where R ₁ is a monovalent organic radical having from 6 to 30 carbon atoms;

R ₂ is a bivalent alkylene radical having from 2 to 4 carbon atoms;

Z is a saccharide residue having from 5 to 6 carbon atoms;

b is a natural number from 0 to 12;

a is a natural number from 1 to 6.

Such a composition can be stored for a long time, and before use it is dissolved in water to obtain a working solution. Due to the use of low-toxicity alkyl polyglucosides in this water-soluble biocidal composition, the claimed preparations can be used for treating mucous surfaces in dentistry, ophthalmology, etc., for hygienic hand disinfection

The disadvantage of this biocidal composition is the low stability of dilute aqueous solutions, which in many cases must be stored for a long time, for example, when used for hygienic disinfection of hands in kindergartens and schools, social welfare institutions, communal facilities, catering establishments, etc. d.

The present invention is the creation of new disinfectants based on complex iodine compounds with a wide spectrum of antimicrobial activity, non-irritating and non-traumatic skin, stable when stored in dilute solutions.

The problem, according to the invention, is solved by the fact that the proposed composition containing the inclusion complex of iodine with cyclodextrin and / or its derivatives, iodine, a source of iodide ions, an organic solvent and, if necessary, a surfactant and / or functional additive, as well as water at the following ratio of components in wt.%:

- iodine 0.03-2.00

- source of iodide ions 0.01-1.00

- cyclodextrins and / or their derivatives 0.03-30.00

- organic solvent 1.00-30.00

- surfactant and / or functional additive - not more than 20.0

- water - the rest

Cyclodextrins can be used in the form of α-, β-, γ-isomers (α-CD, β-CD and γ-CD, respectively) or their derivatives - ethylene glycol (EED), propylene glycol (PEID) or methyl (MECD) esters, as well as their mixtures.

For stabilization, organic solvents are introduced into the composition, which can be used ethyl alcohol (ET), propylene glycol (PG), glycerin (GL), or mixtures thereof.

Block copolymers of ethylene oxide and propylene oxide (BS), polyethylene glycols (PEG), polyethylene glycol esters of α-tocopherol (ferol), or mixtures thereof, can be used as surfactants in the composition.

As functional additives, vitamins E, F, herbal extracts (for example, chamomile extract), antifoam (PG), or mixtures thereof are used.

Cyclodextrins belong to cyclic polysaccharides and have a number of specific properties associated with the peculiarities of their structure. The abundance of hydroxyl groups with their lone pairs of electrons of oxygen atoms creates a high electron density along the entire molecule, which explains their easy formation of inclusion compounds.

This property of cyclodextrins in combination with the presence of a solvent and other ingredients in the composition makes it possible to obtain a disinfectant that has a wide spectrum of antimicrobial activity and is stable when stored in a wide temperature range.

Iodine complexes with cyclodextrins can be obtained by one of the known methods described for the preparation of inclusion complexes with cyclodextrins: when heated in solution, by mechanical grinding of components in a mortar, etc. [Szejtiy JJMater.Chem., 1997, 7 (4), 575 -587].

The disinfectants obtained on the basis of iodine inclusion complexes with cyclodextrins have a wide spectrum of antimicrobial activity in low concentrations, do not irritate or injure the skin, are not toxic, and are stable when stored in dilute solutions over a wide temperature range.

The inventive disinfectants can be used to handle the hands of surgeons and medical staff, for hygienic disinfection in medical institutions, in kindergartens and schools, in social welfare institutions, in public facilities, catering, food industry, food trade, in rural economy, in everyday life with bacterial infections (including tuberculosis), viral and fungal etiology.

The invention is illustrated by the following examples.

Example 1

1.5 g of γ-cyclodextrin in the form of a white crystalline powder and 0.1 g of vitamin F are added to 0.3 g of metallic iodine. The mixture is carefully triturated in a mortar at room temperature. The obtained complex of inclusion of iodine with ethylene glycol ether of β-cyclodextrin is dissolved in 94.9 ml of water, 0.2 g of potassium iodide and 3 g of propylene glycodia are added with stirring. A clear red-brown preparation is obtained.

Example 2

In a liter flask equipped with a stirrer, thermometer, reflux condenser and loading funnel, 1.25 g of iodine, 5 g of β-cyclodextrin propylene glycol ether, 0.6 g of potassium iodide, 10 g of ethanol, 5 g of ferol and 74.1 g of water are placed , heated with stirring to 50-55 ° C and maintained at this temperature for 5 hours. Get the drug in the form of a clear solution of red-brown color.

Examples 3-14.

Disinfectant compositions were prepared under the conditions of Example 2, but with a change in both the composition and the ratio of the components. The composition of the compositions is shown in table 1. All of the compositions shown in table 1 are stable in the temperature range from 0 to 60. Shelf life of at least 3 months.

Table 1. №№
pp Iodine g Cyclodextrin A source
iodide ions Org solution Surfactant Function. additive Water g name g name g name g name g name g 1. 0.30 γ-CD 1,5 Ki 0.20 PG 3.0 - VitR 0.1 94.9 2. 1.25 PETZD 5,0 Ki 0.60 ET 10.0 ferol 5,0 PG 0.001 78.1 3. 0.12 α-CD + β-CD + γ-CD 1.5 NaI 0.05 ET 1,5 BS 1,0 Er 5,0 90.8 4. 0.25 EECD 2.0 NaI 0.12 GL + ET 10.0 PEG 2.0 - - 85.6 5. 0,03 β-CD + PET 1,5 KI + NaI 0.01 PG 2.0 BS 0.5 Vit F + ER 5,0 91.0 6. 0.25 γ-CD 1,0 Ki 0.12 ET 10.0 ferol 0.5 Er 5,0 83.1 7. 0.12 PETZD 0.6 Ki 0.05 GL 5,0 - - Vit. E 0.2 94.0 8. 0.25 β-CD 4.0 NaI 0.12 ET 5,0 ferol 0.5 Vit. E 0.2 89.9 9. 1.30 EECD 30,0 Ki 0.50 ET 5,0 BS 15.0 Vit F 0.5 47.7 10. 2.00 PETZD 20,0 Ki 1.00 ET 10.0 PEG 20,0 PG 0.001 47.0 eleven. 0.50 β-CD + PET 5,0 KI + NaI 0.25 ET 30,0 BS 10.0 Vit F 0.5 53.7 12. 1,50 EECD 10.0 NaI 0.15 ET 10.0 ferol 10.0 PG 0.001 78.3 thirteen. 0.06 PETZD 5,0 Ki 0.01 PG 1,0 - - - - 93.9 14. 0.12 β-CD +
PETZD 1,2 Ki 0.04 GL 5,0 - - Vit F + ER 5,0 88.6

Example 15

The study of the antimicrobial activity of disinfectant samples is carried out against the “wild” strains of Staphylecoccus aureus and Pseudomonas aeruginosa.

For testing use only freshly prepared cultures of microorganisms.

To prepare a microbial suspension containing a given number of microbial bodies per unit volume, 20-hour microbial cultures are used.

To evaluate the effectiveness of a disinfectant sample, 100 µl of a disinfectant solution is added to 100 μl of microorganisms. Contamination time: 0 min, 15 min, 30 min, 60 min.

As a control, inoculation of a bacterial suspension of the same dilution is used, treated with: a) a 5% solution of chloramine and b) without treatment.

After incubation, the tubes are seeded with 50 μl of suspension and evenly distributed into plates with erythritol-agar (to determine the colony of the forming units — CFU after disinfection), which are also incubated in an incubator at 37 ° C for 24 hours.

At the end of the incubation period, the number of colonies is counted and the degree of inhibition of the growth of microorganisms is estimated in comparison with the control samples.

The test results are shown in table 2.

Table 2. No. of experiments from the table. Staphylococcus aureus
CFU, contamination time, min Pseudonwnas aeruginosa
CFU, contamination time, min 1 right away fifteen thirty 60 right away fifteen thirty 60 5 no growth no growth no growth no growth 100 fifty no growth no growth thirteen no growth no growth no growth no growth no growth no growth no growth no growth 7 no growth no growth no growth no growth no growth no growth no growth no growth 4 no growth no growth no growth no growth no growth no growth no growth no growth eleven no growth no growth no growth no growth no growth no growth no growth no growth 2 no growth no growth no growth no growth no growth no growth no growth no growth nine no growth no growth no growth no growth no growth no growth no growth no growth 12 no growth no growth no growth no growth no growth no growth no growth no growth 10 no growth no growth no growth no growth no growth no growth no growth no growth

Thus, disinfectants obtained on the basis of iodine inclusion complexes with cyclodextrins have a wide spectrum of antimicrobial activity in low concentrations, which ensures the absence of irritation and injuries to the skin when they are used. In addition, these drugs are non-toxic, stable when stored in dilute solutions in a wide temperature range.

Due to the described properties, the proposed disinfectants can be used for processing the hands of surgeons and medical staff, for hygienic disinfection in medical institutions, in kindergartens and schools, in social welfare institutions, in public facilities, catering, food industry, food trade , in agriculture, in everyday life with bacterial infections (including tuberculosis), viral and fungal etiology.

Claims (5)

1. A disinfectant containing a complex of iodine with polysaccharides, iodine, a source of iodide ions, a surfactant, a functional additive and water, characterized in that it contains an iodine inclusion complex with cyclodextrin and / or its derivatives as an iodine complex, and also an organic solvent and, if necessary, a surfactant and / or functional additive, in the following ratio of components, wt.%: Iodine 0.03 - 2.00 Source of iodide ions 0.01-1.00 Cyclodextrins and / or derivatives 0.03-30.00 Organic solvent 1.00 - 30.00 Surfactant and / or functional additive Not more than 20.0 Water - The rest. 2. The disinfectant according to claim 1, characterized in that as cyclodextrin it contains α-, β- or γ-cyclodextrin or a mixture thereof. 3. The disinfectant according to claim 1, characterized in that ethylene glycol, propylene glycol or methyl esters of cyclodextrin are used as cyclodextrin derivatives. 4. The disinfectant according to claim 1, characterized in that as an organic solvent it contains ethyl and / or propyl alcohol, and / or propylene glycol and / or glycerin. 5. The disinfectant according to claim 1, characterized in that as a functional additive, it contains vitamins and / or extracts of medicinal plants, and / or antifoam.