RU2208229C1 - Method for diagnosing bacterial infection of urogenital organs system - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves making microscopic analysis of middle portion of urine. A urine drop is placed on transparent plastic surface immediately after urination in the amount of 0.1-0.2 ml and dried at 18-30 C and relative humidity of 55-60% during 18 h. Thin parallel linear structures being found on the dried drop surface, bacterial infection is diagnosed. EFFECT: high reliability of diagnosis. 2 dwg

Description

 The invention relates to medicine, namely to laboratory research, and can be used to diagnose bacterial infection of the genitourinary system.

 Currently, the number of patients with latent (latent) forms of chronic inflammatory diseases of the genital tract, organs of the urinary system (pyelonephritis, cystitis, endometritis, prostatitis) is growing.

 This situation is associated with a number of reasons: the irrational use of antibiotics, the widespread use of hormonal contraceptives that affect the metabolism as a whole, immunity, and the endocrine system. A significant role in exacerbating this problem was played by environmental (radiation, chemical pollution) and social factors (drug addiction, sexual licentiousness, the plight of the bulk of the population).

 It is known that it is difficult to identify patients with latent forms of the chronic inflammatory process in the organs of the urinary and / or reproductive system. With a single urinalysis, small deviations in the analysis are almost leveled by the clinician, and the patient with a chronic inflammatory process, most often with chronic pyelonephritis, remains undiagnosed and falls outside the field of view of the doctor. At the same time, according to statistics, the cause of 1/3 of deceased patients from uremia was chronic pyelonephritis. The prevalence of pyelonephritis in women is noted by many authors. Especially often, the chronic course of pyelonephritis is observed in girls and young women, which finds its explanation in the anatomical conditions and physiological characteristics of the female body.

 Known methods for diagnosing a bacterial infection of the genitourinary system, including microbiological examination of urine culture separated from the genitals (Handbook of Microbiological and Virological Research Methods / Edited by M.O. Birger. M: Medicine, 1973, p. 181-320). These methods make it possible to determine the type of pathogen and the pathogenicity properties of the isolated microorganism strain.

 However, in patients with latent forms of bacterial infection, the clinical picture is erased, and the clinical physician has no caution in prescribing a further examination of the patient.

 The reliability of the study of bactericidal infection by this method is low, it is time consuming, expensive and cannot be widely used.

 Closest to the proposed is a method for the diagnosis of bactericidal infection of the genitourinary system, including a microscopic examination of the average portion of urine (Guidelines for clinical laboratory diagnostics / Edited by V.V. Menshikov. M: Medicine, 1982, p. 121).

 Urinalysis according to Nechiporenko is performed at normal rates of a general clinical analysis of urine in order to detect latent pyuria. To do this, the urine taken from the patient from the middle portion of morning urination is centrifuged, and the sediment is applied to the Goryaev counting chamber, in which leukocytes, erythrocytes and cylinders are counted.

 The disadvantage of this method is the low reliability of the study and the limited scope of use. So, for example, it is not always possible to diagnose a hidden process, since the reaction of epithelial cells of the mucous membranes of the genitourinary tract to the growth and reproduction of microorganisms may be weak or absent altogether.

 The task was to eliminate this drawback and create a technically simple diagnostic method that would reliably detect the growth and reproduction of microorganisms in the study of urine.

This is achieved by the fact that in a method for diagnosing bacterial infection of the organs of the genitourinary system, including microscopic examination of an average portion of urine, immediately after urination, the urine in a volume of 0.01-0.02 ml is applied in the form of a drop onto a transparent surface of plastic, dried at a temperature of 18- 30 ^o C and a relative humidity of 55-60% for 18 hours, and if thin parallel linear structures are detected on the surface of the dried drop, bacterial infection of the genitourinary system is diagnosed.

 Figures 1 and 2 show fragments of dried urine samples during bacterial infection of the genitourinary system.

 The method is as follows.

Take 0.01-0.02 ml of urine from a container into which the average portion of urination has just been collected, apply a drop of urine with a semi-automatic dispenser to a transparent surface of plastic in a volume of 0.01-0.02 ml, dry at a temperature of 18 -30 ^o C and a relative humidity of 55-60% for 18 hours. Then the sample is microscopic in transmitted light, and if in some areas or along the entire surface of the dried drop, thin parallel linear structures are detected, bacterial infection of the genitourinary system is diagnosed.

 Example 1

 Patient P., 24 years old. Complaints of recurrent pulling lower back pain. General urine analysis, according to Nechiporenko - without deviations from normal indicators. In the study by the inventive method, it was found that in the dried urine sample, a section with parallel linear structures (pathways of pathogenic microflora growth) was detected. Conclusion: bacterial infection of the genitourinary system.

 Re-examination of urine taken by a catheter from the organs of the urinary system - the dried urine sample has a section with parallel linear structures. Conclusion: bacterial infection of the urinary system.

 Urine culture showed an increase in Streptococcus faecalis. Further thorough examination showed that the patient has a latent chronic course of pyelonephritis.

 Example 2

 Patient R., 37 years old. There are no complaints. In the study by the inventive method, it was found that parallel linear structures (pathways of pathogenic microflora growth) are determined in the dried urine sample. Conclusion: bacterial infection of the genitourinary system.

 Upon repeated examination of urine taken by a catheter from the organs of the urinary system, the dried urine sample did not have parallel linear structures. Conclusion: there is no bacterial infection of the urinary system. When urine was inoculated, no bacterial growth was detected.

 When examining the secretion of the prostate gland (culture, microscopy of prostate secretion), a picture of chronic prostatitis was established.

 The proposed method is non-invasive, technically simple, the average laboratory assistant can record the results. It can be used as a screening of large populations. Identified patients with bacterial infection of the genitourinary system should undergo a thorough examination using generally accepted clinical and laboratory methods.

 This method will allow you to actively identify patients with chronic forms of bacterial infection of the genitourinary tract, sanitize them and prevent the development of such formidable complications as chronic renal failure, chronic prostatitis and other diseases.

Claims (1)

A method for the diagnosis of bacterial infection of the organs of the genitourinary system, including a microscopic examination of an average portion of urine, characterized in that immediately after urination, urine in a volume of 0.01-0.02 ml is applied in the form of a drop onto a transparent surface of plastic, dried at a temperature of 18-30 ^o With a relative humidity of 55-60% for 18 hours, and if thin parallel linear structures are detected on the surface of the dried drop, bacterial infection of the genitourinary system is diagnosed.

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