RU2182791C2 - Sterilized angiogenine-enriched milk - Google Patents

Abstract

FIELD: dairy industry. SUBSTANCE: sterilized milk contains biologically active supplement including protein fraction which, in its turn, contains up to 1.0 g angiogenine/l. At the same time angiogenine content in ready-to-use product corresponds to 12.5 mg/l. Protein fraction is isolated out of either whole or fat-free milk, subcheesy or curdled whey, ultrafiltrate of either whole or fat-free milk, ultrafiltrate of either subcheesy or curdled whey, colostrum or lately drawn milk. Protein fraction could be introduced both in a liquid or dried form. EFFECT: increased biological availability of sterilized milk. 2 cl, 5 ex

Description

 The invention relates to the dairy industry, namely to sterilized milk enriched with a biologically active additive - angiogenin.

 Sterilized milk is a widespread product of the dairy industry and accounts for about 80% of the total milk production in Russia. In the future, the production of sterilized milk will only increase, because the long shelf life (up to 6 months) makes this product convenient for distribution in the distribution network.

 However, it is known that as a result of sterilization, the mineral composition of milk changes: the content of soluble calcium phosphates and citrates and the mineralization of protein structures decrease, as well as a significant decrease in the content of biologically active substances.

 To increase the biological value of sterilized milk lost as a result of severe heat treatment, attempts were made to introduce biologically active additives in the process of its production. Dietary supplements may contain various micronutrients: complexes of vitamins, minerals, etc.

 For example, the Calcio dairy product is known, which contains calcium and oligofructose, which is necessary for improving the absorption of calcium, developed in Spain in 1999. The product is recommended for elderly people, pregnant and lactating women. In addition to calcium (160 mg / 100 ml), the product contains phosphorus (135 mg / 100 ml), vitamins A, D, E and is produced on the basis of fatty, bold and skim milk. (Tetra Pak Product Review No. 17 - March 1999).

A known sterilized product for children 1-3 years old on a milk basis with vanilla aroma. The composition of the product includes lactose (8%), iron (1.3 mg / 100 ml), zinc, calcium, essential fatty acids, 10 vitamins. Packaging (500 ml) satisfies the daily needs of the child in iron by 65% and 100% in calcium. (Tetra Pak Product Review No. 8 - November 1997)
In addition, the sterilized product Nactalia Ecreme is known, which is skim milk containing 1.2% soluble dietary fiber, iron (1.8 mg / 100 ml) and zinc (1.2 mg / 100 ml). The product is intended for older women who are worried about their health. (Tetra Pak Product Review No. 14 - November 1999).

 However, until now sterilized dairy products are enriched with vitamins, minerals, dietary fibers, but they do not have a biologically active substance - angiogenin. The biological value of sterilized milk is much lower than pasteurized, because it decreases as a result of sterilization, while angiogenin is almost completely destroyed.

 So, only traces of angiogenin were found in sterilized milk. Angiogenin is a protein present in cow's milk, which was first discovered in 1985 (Bond M., Vallee B. U / Biochemistry. - 1988. - Vol. 27 - p. 6282-6287). Angiogenin is known to induce the formation of blood vessels. Angiogenin positively affects a number of biological reactions, including having an immunomodulating effect. It inhibits pathogenic microorganisms. (Angiogenin and the mechanism of angiogenesis / Deadman N.P., Stefanovich L.E. - Novosibirsk: Science. Siberian Enterprise of the Russian Academy of Sciences, 1997). Therefore, it seems interesting to include this valuable biologically active substance in sterilized dairy products to improve the micronutrient supply of the population.

 The present invention is a new dairy product - sterilized milk enriched with angiogenin. The problem is solved by obtaining a sterilized product, including as a biologically active additive, angiogenin in an amount of up to 12.5 mg / L.

 Preferably, sterilized milk contains angiogenin in an amount of up to 8 mg / L.

 Preferably, angiogenin is added to the target product as a protein fraction enriched in it.

 The protein fraction enriched in angiogenin can be obtained from whole or skim milk; cheese or curd whey; ultrafiltrate whole or skim milk; ultrafiltrate of cheese or curd whey, as well as from colostrum or old-milk.

Depending on the type of raw milk used, the fraction contains up to 1.0 g / l of angiogenin, the amount of which is controlled by the quantitative method for determining angiogenin in biological raw materials (RF Patent 2110066, IPC ⁶ G 01 33/04, 1998).

 Preferably, the protein fraction in liquid form contains angiogenin in an amount of from 0.1 to 1.0 g / L.

 The protein fraction can be added to the target product in liquid or dry form.

 The protein fraction enriched in angiogenin is introduced into the target product after the milk sterilization stage to restore the beneficial properties of milk lost due to angiogenin due to sterilization.

 The invention is explained in more detail in the following examples.

 Example 1. The protein fraction containing angiogenin in an amount of 0.15 mg / ml, obtained by ion exchange chromatography using a carboxymethyl cellulose sorbent (CMC) from whole milk, contained 2.8 μg / ml lysozyme, 1.3 mg / ml RNase, 0 15 mg / ml peptides. The protein composition was checked by polyacrylamide gel electrophoresis, the protein nativeness by enzymatic activity (lysozyme and RNase) and by interaction with a placental inhibitor (angiogenin). In aseptic conditions, a protein fraction enriched in angiogenin was added to the homogenized sterilized cooled cow's milk in liquid form. The amount of the introduced fraction was 54 ml / l of sterilized milk. The angiogenin content in the target product was 8.1 mg / L.

 Example 2. The protein fraction containing angiogenin in an amount of 0.125 mg / g, obtained by ion exchange chromatography using a sorbent of carboxymethyl cellulose (CMC) from skim milk. In aseptic conditions, the protein fraction enriched in angiogenin was added to the homogenized sterilized cooled cow's milk in dry form. The amount of the introduced fraction was 64 g / l of sterilized milk. The content of angiogenin in the target product was 8.0 mg / L.

 Example 3. The finished product is obtained according to the procedure described in make-up artist 2, characterized in that the protein fraction containing angiogenin in an amount of 0.09 mg / g, obtained from ultrafiltrate of whole or skim milk, was made in dry form in the amount of 138.9 g / l sterilized milk. The angiogenin content in the target product was 12.5 mg / L.

 Example 4. The finished product is obtained according to the procedure described in example 2, characterized in that the protein fraction containing angiogenin in an amount of 0.15 mg / g, obtained from ultrafiltrate of cheese or cheese whey, was made in dry form in the amount of 83.3 g / l sterilized milk. The angiogenin content in the target product was 12.5 mg / L.

 Example 5. The finished product is obtained according to the procedure described in make-up 1, characterized in that the protein fraction containing angiogenin in an amount of 1.0 mg / l, obtained from colostrum or old-milk, was made in dry form in an amount of 7 ml / l sterilized milk. The angiogenin content in the target product was 7.0 mg / L.

 The energy value of the sterilized milk enriched with angiogenin obtained according to the invention was 581.37 kcal. The biological value of the target product was characterized by amino acid soon. The amino acid rate of the essential amino acid valine was 94.6%, isoleucine - 90.5%, leucine - 123.1%, lysine - 123.8%, methionine - 113.6%, threonine - 125.3%, phenylalanine -158, 9%. The limiting amino acid in this case, as in traditional sterilized milk, is isoleucine. Amino acid composition was determined by known methods (Mondno A "A new system of automatic amino acid analysis" J. Ohromatogr. 41, 156-162. 1969).

 Digestibility of the target product is higher than traditional sterilized milk.

 Thus, the target product according to the invention is not inferior in energy and biological value to traditional sterilized milk, and also additionally contains a valuable biologically active substance - angiogenin obtained from dairy raw materials.

Claims (3)

 1. Sterilized milk containing a biologically active additive, characterized in that it as a biologically active additive includes a protein fraction containing up to 1 g of angiogenin in 1 l, while the angiogenin content in the finished product is up to 12.5 mg / l.  2. Sterilized milk according to claim 1, characterized in that the protein fraction is isolated from whole or skim milk, whey or curd whey, ultrafiltrate of whole or skim milk, ultrafiltrate of raw or curd whey, and also from colostrum or old-milk.  3. Sterilized milk according to claim 1, characterized in that the protein fraction is introduced in liquid or in dry form.