RU2147438C1 - Method for stabilizing soluble state and antibacterial activity of pectin - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves preparing a corresponding portion of dry pectin and a portion of one of acids from Krebs cycle in the amount of 0.6-1.0 g per 100 ml of solvent. The both portions are emptied into water at 80-90 C and solved by means of mixer at a rate reaching 3000 rev/min during 7-10 min. The ready solution is packaged and pasteurized in boiling water bath or flowing damp during 20 min. Then the solution is cooled in the air. The solution is stored in refrigerator at 5-10 C during at least three months. EFFECT: enhanced effectiveness of the method. 2 tbl

Description

 The invention relates to the field of medical and veterinary microbiology, and in particular to means of influencing the causative agents of intestinal diarrheal infections and can be used in medical and veterinary medical institutions.

 Known for the use of pectin for therapeutic nutrition in chronic and acute intoxications with heavy metals and radionuclides. In the gastrointestinal tract, pectin is able to form stable insoluble complexes with heavy metal ions (lead, mercury, etc.), as well as with radionuclides, such as strontium and cesium. These complexes are naturally removed from the intestine, thereby achieving the release of the body from the toxic effects of these substances. See the article Trakhtenberg I.M. and others. "On the prophylactic use of pectin in professional mercurialism," Journal of Occupational Health and Occupational Diseases, 1980, N 7, p. 33 - 36 (1).

 Also known are “Methodological Instructions for the Use of Pectins and Pectin-Containing Products for Medicinal and Preventive Use” N 5049-89 of the Ministry of Health of the USSR, USSR Academy of Sciences and the State Agro-Industry of the Ukrainian SSR, see Urozhay magazine, Kiev, 1990, p. 16 (2).

 The use of pectin for clinical nutrition in acute diarrheal infections is also known. For this purpose, use a 3 - 5% pectin dilution, see "Method for the treatment of acute intestinal diarrheal infections", description of the invention to the USSR patent N 1790412, class. A 61 K 35/78, published in BI N 3, 01/23/93 (3).

 Finally, the known "Pectin preparation for the treatment of diarrheal infections of humans and animals", a description of the invention to the patent of the Russian Federation N 2108106, CL A 61 K 35/78, published in BI N 10, 04/10/98.

 The above method (3) and pectin preparation (4) have no analogues in the world practice of treating infectious diarrheal conditions and are a highly effective means of treating them.

 However, the successful use of pectin for clinical nutrition in intestinal diarrheal infections is regulated by the state of aggregation of pectin - the ability to remain in a dissolved state without subsequent gelation.

 The aim of the invention is to develop a method of stabilizing a pectin solution with simultaneous stabilization and bactericidal action of pectin for a long time, for example several months.

 The present invention has no analogues in the world.

 The problem is solved by using as a stabilizing factor a solution of any organic acid from the Krebs cycle, in a concentration of, for example, 0.6 - 1.0%, in which pectin is dissolved. See Tyukavkina N.A., Baukov Yu.I. "Bioorganic chemistry", M., ed. "Medicine", from 245 - 246 (5).

 Thus, a stable pectin solution is created with a pronounced bactericidal effect over a long period of time, for example, one to two to three months.

 The preparation of a similar aqueous solution of pectin without a stabilizer is accompanied by a gelling effect with the formation of a dense gel after 5-6 hours after preparation. In this form, pectin is not suitable for therapeutic use and no longer has an antibacterial effect.

 The essence of the invention "Method for stabilizing the dissolved state and antibacterial activity of pectin" is as follows.

Prepare an appropriate sample of dry pectin (at a rate of 3-5 g per 100 ml of water) and a sample of acid (at a rate of 0.6 - 1.0 g per 100 ml of water). Then both samples are simultaneously poured into hot boiled tap or distilled water with a temperature of 80 - 90 ^o C and dissolved with a high-speed mixer in the mode up to 3000 rpm for 7-10 minutes. The finished solution is packaged, pasteurized in a boiling water bath or fluid steam for 20 minutes. Then the solution is cooled in air. A pectin solution prepared in this way is suitable for medicinal use for three months. The solution should be stored in the refrigerator at a temperature of 5 - 10 ^o C.

 The effectiveness of the invention is illustrated by the following two examples of studies and observations.

 Example 1

 Two 5% pectin solutions are prepared in parallel: N 1 on tap boiled water without a stabilizer, and N 2 on tap boiled water with the addition of one percent stabilizer.

 After pasteurization, the state of aggregation of these solutions is monitored. The observation results are presented in table No. 1.

 The results presented in table No. 1 indicate stabilization of the solution of N 2, i.e. cooked by the proposed method.

 Example 2

Two 5% pectin solutions are prepared in parallel: on tap water (N 1) and on tap water with the addition of 1% stabilizer (N 2). Then 1 ml of solution N 1 and 1 ml of solution N 2 are mixed with one billion microbial bodies of the causative agents of dysentery or other diarrheal infections. The mixture is incubated in a thermostat at a temperature of 37 ^o C. The viability of the pathogens is checked hourly by seeding on the optimal nutrient medium (nutrient agar, etc.). The results are shown in table No. 2.

 The results shown in table No. 2 indicate a clear bactericidal activity of the solution of N 2.

 Repeated tests with a solution of N 2 after one and two months showed a similar bactericidal activity of an aqueous pectin solution prepared according to the proposed "Method for stabilizing the dissolved state and antibacterial activity of pectin."

 Prepared by the proposed method, a solution of pectin was successfully and with high efficiency used in medical practice in Omsk and the region in the spring and summer of 1998 during an epidemic increase in the incidence of intestinal diarrheal infections.

Literature:
1. Trakhtenberg IM et al. "On the prophylactic use of pectin in professional mercurialism," Journal of Occupational Health and Occupational Diseases, 1980, N 7, 33–36.

 2. "Guidelines for the use of pectins and pectin-containing products for therapeutic and prophylactic purposes" N 5049-89 of the Ministry of Health of the USSR, Academy of Sciences of the USSR, Gosagroprom of the Ukrainian SSR, see journal. "HARVEST", Kiev, 1990, from 16.

 3. "Method for the treatment of acute intestinal diarrheal infections", description of the invention to the USSR patent N 1790412, class. A 61 K 35/78, published in BI N 3, 01/23/93.

 4. "Pectin preparation for the treatment of diarrheal infections of humans and animals", description of the invention to the patent of the Russian Federation N 2108106, class. A 61 K 35/78, published in BI No. 10 04/10/98.

 5. "Bioorganic chemistry", Tyukavkina N. A., Baukov Yu.I., M., ed. "Medicine", from 245 - 246.

Claims (1)

A method for stabilizing the dissolved state and antibacterial activity of pectin, comprising using as a stabilizer a solution of one of the acids of the Krebs cycle containing 0.6 - 1.0 g of acid per 100 g of solvent, which together with a portion of pectin are simultaneously dissolved in water at a temperature of 80 - 90 ^o C and stirring mixer for 7 - 10 minutes at 3000 rev / min, then the solution was ready packed, pasteurized in boiling water bath or flowing steam for 20 minutes and after cooling in air the solution was stored in a refrigerator etc. temperature of 5 - 10 ^o C for at least three months.

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