RU2118986C1 - Method of stabilization of lipids from oxidation - Google Patents

Abstract

FIELD: biotechnology. SUBSTANCE: method involves the use of extract of hardwood of sprouts and/or bark of bush clover (Lespedeza bicolor Turcz) brush. This extract shows both high capability to trap oxygen active radical and inhibitory effect on enzymes of lipoxygenase class. These two mechanisms provides high stabilization of lipids. Method can be used in pharmaceutical, cosmetic, food industry and in medicine. EFFECT: improved method of stabilization, enhanced effectiveness of extract. 3 tbl, 2 ex

Description

 The invention can be used in pharmaceutical, cosmetic, food industry and medicine.

 Known methods of stabilizing lipids to oxidation by introducing into various materials, raw materials and products of antioxidants: tocopherols, naphthols, propyl gallate, phenol, etc. [1, 2], a mixture of polyphenols obtained from juniper and thiodipropionic acid [3].

The disadvantages of the known methods are:
- unsatisfactory organoleptic qualities of products and materials, caused either by the introduced antioxidants or by substances formed under the influence of oxidative processes;
- the possibility of long-term toxic and neoplastic effects of prolonged use of products and materials containing known synthetic antioxidants [4];
- uneven distribution of added antioxidant between the aqueous and lipid phases (plasma membranes, adipose tissues), leading to selective accumulation of the introduced antioxidant additives in some parts of the product and the absence in others.

 As a prototype, a method was chosen for stabilizing mackerel skin lipids, as a fat model system, with a synthetic antioxidant ionol [4].

 However, the known method is ineffective and it has all of the above disadvantages.

 The objective of the invention is to develop a method of stabilization of lipids using a new, non-toxic antioxidant of natural origin, which is more effective.

 The problem is solved in that in the method of stabilization of lipids for oxidation, an extract of lignified shoots and / or bark of a bicolor lespedeza bark (Lespedeza bicolor Turcz) is used as an antioxidant.

 Lespedetsa two-color - a Far Eastern shrub plant, very widespread in the Primorsky and Khabarovsk territories and the Amur region. According to a very conservative estimate, annual harvesting of many hundreds of tons of woodpeckers is possible [5]. Studies have shown that substances with antioxidant activity are concentrated mainly in the bark of the bush. The content of antioxidants in various parts of plants selected at different times of the year, repeatedly carried out by subsequent observations, are given in table. one.

 From the data table. 1, it follows that the accumulation of antioxidants in the bark of the shoots of two-colored woodpecker occurs during the period of drying of the leaves and their high level remains until spring. These data determine the optimal terms for the procurement of raw materials: from November to May.

 The crushed raw materials are subjected to repeated treatment with an organic solvent, for example ethyl alcohol; a mixture of polyphenols, which is obtained by vacuum evaporation and drying, passes into the solution.

 A study of the chemical nature of the antioxidants of the scallop showed that, unlike the green parts of this plant, in wood and bark, one of their main components is caffeic acid and its derivatives. It is known that in addition to the high ability to intercept active oxygen radicals, these compounds are active inhibitors of lipoxygenase class enzymes that contribute to oxidative production spoilage (6). Consequently, the use of extracts of shoots and bark of the lespedean bicolor to stabilize lipids to oxidation is carried out by two mechanisms and should be especially effective.

 The method is illustrated by the following examples.

Example 1. In a Petri dish, various portions of alcoholic solutions of antioxidants are added: ionol, alpha-tocopherol, ethoxyquiline (a Japanese non-food preparation) and an extract of lignified shoots of woodpecker. After evaporation of the alcohol, 5.0 g of refined fat are weighed into cups. The cups are heated to 50 ^° C. pori in a darkened thermostat and weighed periodically. The time elapsed before the increase in fat weight by 2.5% (due to autooxidation) is taken as the induction period.

 The results presented in table. 2, indicate a very high antioxidant activity of lespedeca extracts, not inferior to the best Japanese synthetic drug.

 Example 2. As a substrate for oxidation using lipids of plant origin - a pharmaceutical preparation linetol (ethyl ester of fatty acids of linseed oil). Autooxidation is carried out under the conditions described in example 1, using three antioxidants: alpha-tocopherol, ionol (VNG) and alcohol extract of the bark of Lespedeca bicolor. The results are shown in table. 3, testify to the high stabilizing activity of the new antioxidant, the bicolor lespedeca bark extract.

Sources of information
1. US patent N 2564106, CL. 252-404, 1951.

 2. RF patent N 2077558, cl. C 11 B 5/00, 1997.

 3. US patent N 3784480, cl. 252-404, 1974.

 4. J. Sci. Food Agric., 1983, 34, p. 1154.

 5. Schroeter A. I. Medicinal flora of the Soviet Far East. - M.: Medicine, 1975.

 6. Chem. Pharm. Bull. 1989, v. 37, N 4, 1039.

Claims (1)

 A method of stabilizing lipids to oxidation by the introduction of an antioxidant, characterized in that the extract of lignified shoots and / or bark of two-colored woodpeckers (Lespedeza bicolor Turcz) is used as an antioxidant.

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