RU2117289C1 - Method for diagnosing malignant neoplasm formations - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: method involves taking venous blood sample to obtain native serum that is then placed into a cell for measuring its absorption spectrum in 430-715 nm bandwidth. Then the serum is poured into open reservoir and dried. The dry residue is crushed and mixed with Vaseline oil and infrared absorption spectrum of the sample is taken. The ratio of absorption maximum at 1165 cm^-1 to absorption maximum at 1070 cm^-1 is calculated. Absorption strips at 680 and 715 nm being detected and/or the ratio magnitude being less than or equal to 0.43, malignant neoplasm is to be diagnosed. EFFECT: enhanced accuracy of diagnosis.

Description

 The invention relates to medicine, in particular to oncology, and can be used in the diagnosis of malignant neoplasms.

 Known methods for the diagnosis of malignant neoplasms, in particular, in the liver, based on the registration of absorption spectra in the region of 500-1200 nm biological fluids (blood serum and urine).

 However, the known methods can diagnose malignant tumors of narrow localization [1].

 The closest in the set of essential features is a known method for the diagnosis of lung cancer, which is selected as a prototype.

The known method is as follows. A lung tissue biopsy is taken, placed in a test tube with hexamethyldisilazane and heated at a temperature of 120-140 ^o C for 1-2 hours. The hexamethyldisilazane is drained, the tissue sample is washed with diethyl ether, it is dried in air and infrared absorption spectra of biopsy substances are taken in tablets with potassium bromide or in a thin layer of a substance in the form of a suspension in liquid paraffin. According to the obtained spectra, the ratio of the optical densities of the absorption bands with the maxima at 1160 and 1065 cm ^{–1 is} calculated and, with a value of this indicator equal to 0.49 or less, lung cancer is diagnosed [2].

 This method allows you to diagnose lung cancer with great accuracy (probability of error ≈2%).

 However, the known method allows you to diagnose only lung cancer. In this case, lung tissue biopsy is taken as biomaterial, which is associated with tissue trauma, is fraught with complications and the possibility of implantation metastasis of the tumor.

 In addition, the use of an expensive and unsafe solvent operation from the point of view of the researcher’s health and fire hazard is also proposed here. The duration of the method is 2-3 hours

 The objective of the invention is the ability to diagnose a malignant neoplasm in any organ of a person without injuring organs and tissues, increasing its safety and reducing the duration of the analysis.

The problem is solved by a method for the diagnosis of malignant neoplasms, including taking a sample by drying it and introducing the obtained dry residue into the base, recording infrared absorption spectra (IR spectra) in the range of 1200-1000 cm ^-1 , calculating the ratio of the absorption maximum at 1165 cm ^-1 a maximum absorption at 1070 cm ^-1, (H ₁₁₆₅ / H ₁₀₇₀₎ diagnosing malignancy meaningfully value of this ratio, according to the invention as the biomaterial take native serum and optional tively before registering the IR spectra measured spectrum of its absorption in the region 430-750 nm, in the presence of absorption bands at 680 and 715 nm and / or ₁₁₆₅ value of the ratio H / H ₁₀₇₀ equal to 0.43 and less diagnosed malignancy in humans.

 Distinctive features are that native blood serum is taken as biomaterial and, before recording IR spectra, in addition, the absorption spectrum of native blood serum is measured in the region of 430-750 nm, in the presence of absorption bands of 680 and 715 nm and / or a ratio of 0.43 and less diagnosed with malignant neoplasm in the human body.

 The fact that blood serum is examined as a biomaterial makes it possible to obtain information about pathology, in particular a malignant neoplasm, in any organ, since the blood passes through all organs and tissues, therefore it contains almost all the substances released during the formation and development of a malignant tumor.

 Registration of the absorption spectrum of native blood serum in the region of 430-750 nm allows obtaining additional information on the development of malignant neoplasms in various human organs by the presence of absorption bands at 680 and 715 nm.

It has been experimentally proved that with an H ₁₁₆₅ / H ₁₀₇₀ ratio of 0.43 or less, an oncological disease occurs.

However, the presence of a malignant neoplasm is diagnosed in the presence of absorption bands of 680 and 715 nm and / or the ratio H ₁₁₆₅ / H _{1070 of} 0.43 or less, since information on the development and stage of the malignant neoplasm enters the blood as the sum of certain chemicals whose registration in the range of 430-750 nm and 1200-1000 cm ^-1 can be carried out with different accuracy depending on the structure of these substances.

 The analysis of patent and scientific and technical information showed that the proposed method is new and meets the criterion of "inventive step".

 The proposed method is as follows.

Venous blood is taken from the patient, native blood serum is obtained, which is placed in a cuvette for subsequent measurement of its absorption spectrum in the region of 430-715 nm. Then the serum is poured into an open container and dried. The dry residue is crushed and mixed with liquid paraffin, then the infrared absorption spectrum of the sample is taken, the ratio of the absorption maximum at 1165 cm ^-1 to the absorption maximum 1070 cm ^-1 (H ₁₁₆₅ / H ₁₀₇₀ ) and in the presence of absorption bands at 680 and 715 nm and / or a ratio of 0.43 or less diagnose a malignant neoplasm in the human body. Analysis time 30 min.

When measuring the absorption spectrum in the range of 430-750 nm, bidistilled water is taken as a reference standard. To record the absorption spectrum in the region of 430-750 nm, a Perkin-Elmer-Spectrofotometer Coleman 575 spectrophotometer was used, and IR spectra in the range of 1200-1000 cm ^{-1 were} recorded on Specord-75-IR and " Specord-M80. "

 The proposed method on the basis of the medical unit of the Gorky Automobile Plant (Nizhny Novgorod) was diagnosed 163 people. As a result, 57 people were diagnosed with malignant neoplasms in the body.

Of these, 1 - in 44 people, the spectra had both the presence of absorption bands of 680 and 715 nm, and the value of the ratio H ₁₁₆₅ / H ₁₀₇₀ was 0.43 or less;
2 - in 4 people, the absorption bands in the spectra of 430-750 nm were absent, and the value of the ratio H ₁₁₆₅ / H ₁₀₇₀ was 0.43 or less;
3 - in 9 people, absorption spectra of 680 and 715 nm were present in the spectra, and the value of the ratio H ₁₁₆₅ / H ₁₀₇₀ exceeded 0.43.

 Moreover, all 163 people were diagnosed in parallel with the proposed method using conventional clinical methods for diagnosing neoplasms in the human body, as a result of which malignant neoplasms were detected in 59 people. Therefore, the accuracy of the proposed method is ≈97% (57 of 59 people).

Example 1. Patient Rukavishnikov VK, 53 years old. Medical history N A-563. Clinical diagnosis: central cancer of the upper lobe of the left lung (T ₃ N ₃ M ₀ ).

In parallel with clinical methods, diagnosis was carried out by the proposed method (N 0395 from 03.16.94). As a result, the presence of absorption bands at 680 and 715 nm was noted in the spectra (positive test) and the ratio of the maximums of the absorption bands in the IR spectra of H ₁₁₆₅ / H ₁₀₇₀ was 0.43 (positive test).

 Thus, according to the proposed method, the diagnosis is cancer. Operation performed: extended pulmonectomy on the left. The result of a morphological study is cancer.

 Example 2. Patient Besedina T.N., 41 g. Case history No. B-2120. Clinical diagnosis: cervical cancer (C-r colliuteri tercia).

In parallel with clinical methods, diagnosis was carried out by the proposed method (N 0391 from 03/04/94). As a result, the presence of absorption bands at 680 and 715 nm was noted in the spectra (positive test), while the ratio of the maximums of the absorption bands in the IR spectra of H ₁₁₆₅ / H ₁₀₇₀ was 0.66 (negative test).

 The diagnosis of the proposed method is cancer.

 The result of a postoperative morphological study is cancer.

Example 3. Patient Semin K.I., 64 years old. Medical history N A-215. Clinical diagnosis: lung cancer (T ₃ N ₃ M ₀ ).

In parallel with clinical methods, diagnosis was carried out by the proposed method (N 0430, 0431 from 04/06/94, 0587 from 05/15/94). As a result, the spectra showed the absence of absorption bands at 680 and 715 nm (negative test), while the ratio of the maxima of the absorption bands in the IR spectra of H ₁₁₆₅ / H ₁₀₇₀ according to three analyzes was, respectively, 0.30; 0.32 and 0.28 (positive tests).

 Thus, according to the proposed method, the diagnosis is cancer.

 Operation performed: advanced pulmonectomy. The result of a morphological study is cancer.

 Example 4. Lebedev VV, 49 years. Almost healthy. Clinical methods of disease are not identified.

In parallel with clinical methods, the diagnosis was carried out by the proposed method (N 1307 from 08/10/94). As a result, the spectra noted the absence of absorption bands at 680 and 715 nm (negative test) and the ratio of the maximums of absorption bands in the IR spectra of H ₁₁₆₅ / H ₁₀₇₀ was 0.53 (negative test).

 So about 6 times, according to the proposed method, the diagnosis is not cancer.

 As can be seen from the obtained data, the proposed method allows to diagnose malignant neoplasms in any human organ with an accuracy of ≈97%, which is determined by the presence of at least one or both (of the two used in the method) positive tests.

 The method is non-invasive, safe for the patient and the doctor.

 The duration of the diagnostic analysis is no more than 30 minutes.

 Sources of information.

 1. Gorsky S.M., Ilyicheva K.V. Spectrofluorimetry and spectrophotometry of biological fluids: Abstracts of the IV All-Union meeting "Luminescent analysis in medicine and biology and its hardware" .- M .: February 17-23, 1992, p. nine.

 2. USSR copyright certificate N 1489373 - prototype.

Claims (1)

A method for the diagnosis of malignant neoplasms, including taking a biopsy sample, drying the sample and introducing the obtained dry residue into the base and recording infrared absorption spectra in the region of 1200 -1000 cm ^-1 , characterized in that native blood serum is taken as a biomaterial and an additional spectrum is recorded absorption native serum in 430 - 750 nm and the presence of absorption bands 680 and 715 and / or the value of the ratio of the absorption maximum at 1165 cm ^-1 to the absorption maximum at 1070 cm ^-1, equal nd 0.43 or less, diagnosed malignancy in humans.