RU2105570C1 - Agent for bacterial seeding decrease - Google Patents

Abstract

FIELD: applied microbiology, medicine, veterinary science. SUBSTANCE: invention proposes the use of preparation "Teflex" based on liquid polytetrafluoroethylene. This ensures to decrease bacterial background an average by 2-3-fold. EFFECT: enhanced effectiveness of agent. 1 tbl

Description

 The invention relates to the field of biotechnology, and in particular to substances used to combat bacterial contamination, and can be used in applied biotechnology, medicine, veterinary medicine.

 One of the most important problems in the field of medicine and veterinary medicine is the fight against the bacterial background inherent in hospitals, rooms for keeping birds and pets, etc.

 Currently, for the fight against bacteria, treatment of the premises with drugs such as metaphor, formaldehyde, glutaraldehyde, alkamon, etc. is used. [1] by aerosolizing or washing the infected areas with appropriate solutions. However, these reagents are usually quite toxic, which does not allow working with biomaterial in their presence, require careful ventilation of the premises or the removal of their traces with significant volumes of water.

Under normal conditions, to reduce the pollution of premises with toxic substances with chemical reagents, microorganisms, they use the treatment of indoor walls with waterproof paints and enamels, the replacement of concrete surfaces with metal, the use of special clothes and shoes [2, 3]
The disadvantages of these methods, along with impaired air exchange, is a weak effect on the bacterial background, especially with long periods of operation of the premises.

 The prototype of the invention is the use of a plastic film to fight against the bacterial background, from which the walls of special boxes are made [4] The film is pulled onto special frames from which the camera is assembled for work.

 However, the low operational characteristics of polyethylene, especially during long periods of operation, do not allow to achieve high protection against bacterial flora. In addition, the use of the film leads to a decrease in air exchange, surface fogging and other negative factors. The use of polyethylene for such purposes in livestock complexes is generally unlikely. The disadvantages include the inability to use polyethylene for the disinfection of rubber products, clothing, etc.

 The objective of the invention was the creation or search for drugs that combine convenience and versatility with reliable antibacterial protection.

 As a result of the studies, it was found that the problem can be solved by using Teflex, a drug based on polytetrafluoroethylene (PTFE), as a means to reduce bacterial contamination of premises.

 Currently, PTFE-based compositions are used in radio and electrical engineering as an insulating material, in the chemical industry for the manufacture of pipes, gaskets, valves, in medicine for the manufacture of human prostheses, in the manufacture of bearings, etc. [5-7] The use of PTFE to combat bacterial contamination in the reviewed literature was not found.

 The drug "Teflex" is a composition of 50-85% isopropanol or other alcohol, 8-25% heptane, 2-10% PTFE and 1-8% of operational additives (emulsifiers, dispersants, aromatic additives) and is intended to protect wooden, concrete and stone coatings from water, oil, fats, etc. pollution.

The effect of various samples of the Teflex preparation was studied by the example of a culture of E. coli bacteria M-17, grown on glucose-mineral medium to a density of 1.5 • 10 ⁹ cells / ml. When 0.1 ml of a preparation containing 76% isopropanol, 20% heptane, 2% PTFE and 2% additives was introduced into the culture, after a day of cultivation at 37 ^° , the growth inhibition zone around the well with the preparation was 2-2.5 mm.

 When treated with a preparation containing 85% amyl alcohol, 6% PTFE, 1% additives and 6% heptane, by spraying for 1 s from a distance of 15 cm, the diameter of the colonies on the experimental plates was 1.5-2 times less than on the control .

 When treating cups in the same mode with a preparation containing 10% PTFE, 25% heptane, 8% additives and 57% butanol, a 10-fold decrease in the number of colonies was observed compared to the control.

 When lawns with wells were introduced into the culture-seeded cells, 0.1 ml of the preparation containing 4% PTFE, 10% heptane, 1% additives and 85% amyl alcohol was added, and a growth inhibition zone around the 2.5-3 mm wells was observed.

 The introduction of the drug to the surface is carried out, as a rule, from aerosol cans containing up to 40% propane (usually 30-40%) or using tampons.

 Before applying the drug, the surface is cleaned of impurities, dried well, after which the drug is applied to it, dried for 4-5 minutes, and then rubbed and polished.

The use of the Teflex preparation for tissues is carried out by introducing the composition into water during rinsing at the rate of 100 ml per 1 kg or by spraying on the surface until the material is evenly moistened, then the fabric is ironed with a hot iron at a temperature of 100-120 ^o C.

 The effectiveness of the effect of the drug on the contamination of various surfaces was carried out by the study of swabs from the surface treated by it. The test results are shown in the table.

Studies of the bactericidal action of the Teflex preparation were carried out by the example of culturing E.coli cells M-17 in flasks on agar, to which 0.1 5.0% of the drug was added. The evaluation was carried out according to the results of cultivation on a rocking chair at 37 ^o C for 7 hours. The biomass yield after centrifugation in the control was 5.2-0.2 g. When the drug was administered in a dose of 0.1%, 4.4-0.4 g. at a dose of 1.0% 3.9 0.6 g, 5% 3.5 0.9 g.

 The experiments showed that the Teflex preparation, when used to reduce bacterial contamination, combines moderate bacteriostatic properties with a significant reduction in adhesive properties. The indicated qualities at high operational indicators: good protection against humidity, lack of fogging, non-formation of a film that impedes the “breathing” of wall material, fire safety, etc. make the drug promising for use in treatment and prevention centers, livestock complexes, research institutes and enterprises of the microbiological industry.

Sources of information
1. Veterinary drugs. Directory. Ed. A.D. Tretyakova. M. Agropromizdat, 1988, p. 66-75.

 2. Safety in the microbiological industry. Ed. V. M. Tsygalnitsky. M. Forest industry, 1988, p. 83-85.

 3. Personal protective equipment. Directory. Ed. S.A. Kaminsky. L. Chemistry, 1989, p. 54-212.

 4. Technical passport for the K-4 dedusting chamber, Tissa software. Uzhgorod, 1988.

 5. Fluoropolymers. Ed. Walla. M. 1975.

 6. M. Lazar, R. Pado. Ftoroplasty. M.-L. 1965.

 7. Advertising of the company "Softprotector". St. Petersburg, 1985, Teflex.

Claims (1)

 The use of the drug "Teflex" based on liquid polytetrafluoroethylene as a means to reduce bacterial contamination.

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