RU2090198C1 - Method of preparing biologically active substances with antifungal activity - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: pollen and rosettes from Tien-Shan ferula pollination were extracted with olive or sunflower oil for 3 days at the room temperature, then on water bath at boiling for 35-45 min at ratio raw : extract = 1: 10. Biologically active substance is used as ointment at fungal diseases, allergic rash, trophic ulcer, hemorrhoid and can be added to cream for skin and other cosmetic preparations. EFFECT: improved method of preparing, enhanced effectiveness.

Description

 The invention relates to medicine and relates to the production of biologically active substances with antifungal activity.

 A known method of producing a biologically active substance from eucalyptus in the form of a 2% oil solution. Galenic forms from eucalyptus leaves are used as antiseptic agents, in dermatological practice for various pustular diseases.

 The target products obtained in the known method are not active enough.

 The purpose of the invention is the increase in activity of the target product and the expansion of its scope.

 To achieve this goal, pollen with rosettes after pollination of ferula (Ferula) of the Gorno-Tien Shan is extracted with oil for 3 days at room temperature, and then when boiling in a water bath for 35-45 minutes. For extraction of raw materials: the extractant is taken in a ratio of 1:10.

 Ferrule is used for extraction.

 The genus Ferula is a perennial herbaceous plant, geophyte, with a short annual period of growth and development and a long dormant period occurring in an unfavorable season.

 A hot and dry summer is such a period in Central Asia and Kazakhstan, as in most other parts of the range of the genus. Vegetation of ferrule begins mainly in the early spring and ends at the very beginning of summer, at the same time the generative phase ends. In the fall, as a rule, does not vegetate. The period of annual vegetation is especially short. The short duration of the growth period per relatively wet spring is compensated by the significant intensity of growth processes. The growth of the generative shoot is 14-16 cm per day. According to the features of the passage of a large life cycle, this type of ferrule belongs to the biological group of Monocarpics. Monocarpics bloom once at the very end of life and after fruiting completely die off (over 50 years). This plant belongs to the type of ferrule. The Kazakh Institute of Botany has previously been given the name "Gorno-Tien Shan."

 In the pregenerative part of the life cycle, this plant annually forms a rosette of leaves. The number of leaves in it constantly increases with the age of the individual.

 The biennial plant has one leaf, three-year 1-2, four-year - 1-2, five-year 3, six-year 2-4, seven-year 6-7, eight-year 9-11, nine-year 10-12, ten-year 12-14, then throughout the period In life, the number of leaves does not increase, and root growth annually (by weight) increases markedly. In this species, there is only a monopodial increase in the axial shoot, first a shortened vegetative, and in the last year an orthotropic above-ground. This shoot thus belongs to the polycyclic category, and the plant to the semi-rosette category. Only in case of damage to the apical kidney can 2-4 generative shoots sometimes form, but even in this case, Monocarpy is not disturbed.

 The underground part of the ferrule is often very massive and complex. It consists of a root and stem root (caudex), which, in turn, is a complex formation of stem and root parts by origin.

 The stalk root consists of a heavily grown underground shortened stalk of a vegetative rosette shoot that goes down to the equally overgrown part of the (basal) root.

 Hypocatilia is also involved in the formation of the stem. On the surface of the stem, scars and folds are clearly visible, firstly, at the place of attachment of leaf petioles of previous years, and, secondly, as a result of contractility of the roots.

 The roots of the ferrule are covered with a slightly brown or black crust (depending on the soil, sandy, chernozemic), easily removable, under which there is a soft tissue filled with a specific white resin. It tastes with a astringent, bitter taste and has a high viscosity.

 The stems of the ferrule reach from 1.5 to 2.0 m in height, the diameter of the stem at the base is from 3 to 10 cm, regardless of the height of the stem. A characteristic feature of the stems of the ferrule is the swelling of the nodes. Branching begins in the middle. The anatomical structure of the stem is similar and typical of the umbrella family in general.

 Unlike other ferrules with ternary dissected leaves, the Gorno-Tien Shan ferrule has five-dissected leaves with a well-defined petiole that enters the bosom, and is characterized by fine-leafed leaves. The inflorescence is complex golden-colored.

 During flowering of the Gorno-Tien Shan ferrule, it is necessary to monitor the pollination of plants. Immediately after pollination of female inflorescences, male inflorescences, together with pollen and a socket, are removed into plastic bags and delivered to a processing point. During the first days after removal, the plant material is processed.

 The proposed invention is illustrated by the following examples.

 Example No. 1.

 50 g of pollen, along with crushed rosettes, is poured into 500 g of olive or sunflower oil and kept for 3 days at room temperature, protecting from direct sunlight. Then the extraction is carried out in a water bath at a boil for 35 minutes Shelf life of the finished product is 5 years.

 Example No. 2.

 80 g of pollen with crushed rosettes pour 800 g of olive or sunflower oil and incubated for 3 days, protecting from direct sunlight. Further extraction is continued in a water bath at the boil for 40 minutes. The shelf life of the drug is 5 years.

Example N 3
100 g of pollen, together with crushed rosettes, is poured into 1 liter of olive or sunflower oil and kept for 3 days at room temperature. Then the extraction is carried out in a water bath at the boil for 45 minutes.

 Shelf life of the finished product is 5 years.

 Obtained by the proposed method, the biologically active substance is used in the form of an ointment for fungal diseases. Good results were obtained in the treatment of allergic rashes, trophic ulcers, hemorrhoids.

 A biologically active substance isolated from pollen and rosettes after pollination is introduced as an active additive for the skin into creams and other cosmetic products.

Claims (1)

 A method of obtaining biologically active substances with antifungal activity by extraction of plant materials with oil followed by isolation of the target product, characterized in that, in order to increase the activity of the target product by expanding its scope, pollen with Ferrula rosettes after pollination is used as plant materials oil extraction is carried out for 3 days at room temperature, then in a water bath at a boil for 35 45 minutes, while the raw material extractant is taken in the ratio 1 10.