RU2053783C1 - Agent for treatment of wound, ulcer, burn - Google Patents

Abstract

FIELD: medicinal preparations. SUBSTANCE: method involves the use of eracond as a stimulating agent of organism physiological functions which represents condensed plant extract. EFFECT: enlarged raw base of medicinal composition preparing.

Description

 The invention relates to herbal medicines obtained using biotechnological methods, and can be used to treat surgical and trauma wounds, thermal, hydrothermal, chemical, radiation burns, and also as a dermatological and antiseptic agent that helps alleviate the severity of the course of infectious processes.

The stimulating effect of fetal blood serum is known (Pathological physiology, 1986, N 5, pp. 28-31), due to its main protein component a-protein (AFP). The biological activity of another embryonic protein of trophoblastic β ₁ -globulin (TBH) has been established. The body's immune responses are actively involved in the process of reparative regeneration under the humoral influence of, for example, embryonic proteins and proteins of the “acute phase” C-reactive protein (PSA), which plays an important role in tissue repair.

 The ability of a mummy to accelerate regenerative processes (Altymysheva A.A. Korchubekova V.K. What do we know about mummy, 1974) is a prototype.

 Known pharmacological regulation of adaptive and compensatory reactions of the body using immune-active compounds. Among them, plant polysaccharides, for example, the polysaccharide of palustran, which is used to induce the induction of cytological T-lymphocytes in a unidirectional culture, are of the greatest interest. When it is administered to animals in doses and modes that cause maximum stimulation of the release of cytological T-lymphocytes into the blood, palustran effectively inhibits the growth of sarcoma-180, adenocarcinoma of the mammary gland Ca-755.

 A known method of autosw. N 1028335, 1981 for obtaining a fraction of iridoids with the ability to increase the physical condition of healthy people in specific conditions (for example, in zero gravity, harsh climate, etc.), as well as in diseases accompanied by decreased working capacity, lethargy, and adynamia.

 According to the known invention, a biologically active substance is obtained from the mullein plant Dzhungarian.

 A known extract from chicory roots is a biologically active substance with a hepatotropic effect (ed. St. N 948385, 1980). The dry extract provides an increase in the number of dividing cells in the organs of the tested animals, normalizes the content of total protein and albumin fraction in the blood.

 A tool is known that has the ability to increase the efficiency of the body (ed. St. N 858833, 1980) on the basis of polyphenolic compounds from the roots and rhizomes of the blood sanguine sorba Z. The tool also has antihypoxic and stimulating effects.

 Known pharmaceutical composition according to the application of Germany N 0S 3545620, 1987, designed to improve natural functions, containing finely divided leaves as an active component.

 Known drug composition according to PCT application N 88/03406, 1988, containing as an active principle alpha-linolenic acid and alcohol of vegetable origin. This medication is suitable as an anti-inflammatory.

 Known blackcurrant leaf extract obtained by treatment with aqueous alcohol, having anti-inflammatory activity, accompanied by analgesic action (application of France N 2624737, 1989).

 In animal experiments, it was established (Pharmacy, 1988, v. 37, No. 6, pp. 36-38) that the extract of the sylpha przepnoznolistnoy plant feed promotes the healing of burn wounds: complete epithelization of the surface by the 16th day (in the experiment), instead of 20-25 days in control. The extract is used as an ointment.

 A new antiseptic is known for the local treatment of superficial burns (Burns, 1986, 12, No. 7, pp. 518-520) of inadia with ointments based on oil distillation products.

 The use of mummies for the treatment of patients with deep burns is known. Mumiye is applied topically in the form of a 1-3% solution or ointment with the addition of novocaine, and also orally at a dose of 0.5 g once a day for 10 days. This helps to reduce pain, the disappearance of inflammation, reduce the time of rejection of the scab and cleanse the wound of necrotic tissue, the appearance of pink granulations, accelerates epithelization.

 It is known to use spermaceti for the treatment of burn lesions, which causes earlier and more pronounced signs of regeneration of epidermal elements (spermaceti oil and regeneration).

 The following composition is used, spermaceti 28.0, spermaceti oil 70.0, anesthesin 1.5, dicain 0.5.

 A known method of obtaining anti-burn funds (ed. St. N 543399, 1975) by extraction of plant materials with an organic solvent. Plant materials: rhizomes of Kalgan, buds of black poplar, periwinkle leaves and calendula flowers. Studies on animals (dogs) showed that the drug has pronounced analgesic properties, limits lymph and blood loss, reduces the absorption of toxic tissue decay products, provides drainage of wound secretion, accelerates epithelization, and promotes the influx of leukocytes to the wound surface, forming a granulation shaft at the border of necrotic and healthy tissues, contributes to the rapid rejection of necrotic masses and cleansing wounds.

 In experiments on rats, the healing of skin wounds under the influence of serum blood preparations was studied (pathological physiology, 1986, No. 5, pp. 28-31), which affect the regeneration of skin wounds under the influence of industrial preparations of placental albumin and blood serum of patients with thermal skin damage. The stimulating effect of placental albumin on recovery processes is explained by the presence of α-fetoprotein in it, which is involved in the processes of cell division.

 Known stimulation of wound healing using epidermal growth factor ("Surg", 1986, 203, N 4, S. 374-281). Epidermal growth factor (EGF) is a polypeptide that improves proliferation by enhancing DNA synthesis, activating RNA, stimulating the formation of proteins and extracellular macromolecules.

 The use of total pine oil for local treatment of purulent wounds has been experimentally substantiated (manuscript of the dep. At the All-Russian Scientific Research Institute of Health Sciences of the USSR N 11756-86). The effect of a 10% emulsion of pine essential oil on wound healing was tested in experiments on white rats. It was found that a 10% emulsion of pine essential oil has a fairly pronounced antimicrobial, anti-inflammatory, necrolytic and deodorizing effect.

 A known method of obtaining funds with wound healing activity according to ed. St. N 1228861, 1984, by extraction of angelica, creeping alcohol, and evaporation of the extract.

 Known medicinal composition according to the application EPP N 0321709, 1989, which is a series of substances, among which the resin from the bark of the plant Mimosa Tuitenuiflora leguminosa Poir.

 A well-known drug according to the application ЕВП N 0324227, 1989, which increases the intracellular concentration of ATP, the level of physical ability and accelerates wound healing.

 Known non-toxic germicidal and wound healing preparations according to the application ЕВП N 0293498, 1988, containing propylene glycol, allantoin and extract obtained by soaking the stem and leaves of the plant Symphytym officinale in propylene glycol.

 A known composition for accelerating wound healing according to PCT application N 88/03029, containing bacterial exotoxin as an immunostimulant.

 Known drug for scarring wounds based on natural vegetable oil according to the application of France N 2591112, 1987.

 Known tool (ed. St. N 1179998, 1983) for the treatment of purulent-inflammatory diseases of the skin and soft tissues gel "fusidium".

 Known anti-inflammatory and antipruritic agent "Chrysarobin", which has a stable, anti-inflammatory and resolving effect. The drug is made from the roots of Tanshan sorrel and can be used in the treatment of eczematous process, scaly lichen and fungal infections.

 Known drug for the regeneration of damaged tissue, as well as for the treatment of dermatitis, otitis media, gingivitis, periodontal disease and tonsillitis. The drug as a dermatological and antiseptic agent contains in 1 part 0.025-0.50 h of a purified propolis flavonoid complex (ed. St. Czechoslovakia N 253424, 1987).

 Known medicinal ointment (ed. St. Czechoslovakia N 253629, 1987) based on an oil-in-water emulsion, contains standard aqueous ethanol extracts of plantain, thyme and calendula as main components. The ointment is intended for skin care and treatment of damaged skin.

 Known plant extract Polygonum aviculare for topical use in a water-oil or physiological or organic solvent. The drug is suitable for the treatment of eczema, acne, skin irritation, bleeding from the gums and lesions of the mucous membrane (UK application N 2178954, 1987).

The disadvantages of the known drugs for the treatment of surgical and trauma wounds, various burns, allergic dermatitis are:
limited raw material base of plant materials;
sophisticated manufacturing technology;
high cost;
limited therapeutic effect.

 To this end, as a biologically active substance of the agent, which has the ability to stimulate the physiological functions of the body by acting on immune responses through complex mechanisms of humoral regulation, condensed plant extract from alfalfa (alfalfa hay) is used.

 The significance of the differences of the invention is as follows.

 The drug composition meets the criteria of the invention of "novelty."

 The positive over-total effect of the Heraconda-based drug composition is as follows. The prototype of Erakonda is considered the natural substance mummy is a scarce and expensive substance. Its reserves in nature are limited.

 Analogs spermaceti, sea buckthorn oil, fish oil, water extracts of plants are either very expensive agents, or agents that do not have a large therapeutic effect.

 Condensed vegetable extract (Herakond) is a preparation obtained from alfalfa (alfalfa hay), treated with an extractant containing soluble metal salts in mg per 1 kg of plant mass, for example, in the following ratio, mg: Mo 8.0; Ba 10.0; Pb 20.0; C 1.05; V 1.0; Cz 0.5; Zn 200.0; Fe 300.0; Sn 40.0 (ed. St. N 1839723, 1989).

 An aqueous solution of Heraconda was prepared as follows. The resinous preparation Erakond was dissolved in distilled water until completely dissolved, filtered through several layers of gauze. The aqueous solution was used once. Unused aqueous solution of Herakonda is not subject to storage.

 An oracle based ointment was prepared as follows. Heracond was dissolved in distilled water (3-5% or 8-10% solutions), thoroughly stirred, filtered. Dimexide (1-20%) was added to the solution. The basis of the ointment was the fuse of vegetable oils (up to 100% of the volume). The heated composition was thoroughly mixed until a homogeneous mass was formed. The ointment can be stored in a household refrigerator and reused.

 It is known that the development of radiation injuries of the skin is due to a degenerative change in blood vessels. Thrombosis occurs in medium and large vessels, and with late radiation ulcers, sclerotic changes and haalinosis of the vessel walls, which leads to a violation of regional blood circulation and lymphocirculation, which determine the transition of the process of radiation damage to chronic. In addition, ionizing radiation affects nerve fibers and their endings in the skin.

 Local treatment of various skin lesions should consist in the use of drugs that have antimicrobial properties and stimulate the processes of tissue regeneration and epithelization.

 The regeneration processes that characterize tissue repair depend on various factors.

 Arising as a local manifestation of the reactive properties of the body, reparative regeneration is determined by the general condition of the body: central nervous system function, humoral-hormonal effect, the level of immunological and metabolic processes, the state of innervation and vascularization of damaged tissues, the development of infection, etc.

 Therefore, it is necessary to have a drug with a wide range of therapeutic effect and giving a pronounced positive effect. Such a drug is Heracond. The drug is not expensive, it is stable during storage, it does not require preliminary sterilization, it is easy to use, it can be combined with other means and methods of treatment, it is not toxic, it has high biological activity.

 This is explained both by the nonspecific effect of a highly effective product that lubricates and softens the affected surface and promotes penetration of the drug through the epithelium, and by specific influences aimed at enhancing repair processes as a result of patient accumulation of intracellular reserves of macroergs, enhancing the processes of protein synthesis, improving and increasing respiration of regenerating tissues .

 The development of a method for treating wounds was carried out on the basis of the Trinity Veterinary Institute in a surgical clinic for the healing of postoperative wounds on the ventral abdominal wall in piglets after hernia repair.

 The control experiment was carried out on 14 gilts with a live weight of 22-28 kg each, divided by the principle of analogues of 7 goals in two groups. All animals had the same conditions of keeping and feeding.

 After carrying out complete surgical preparation according to the generally accepted technique, the animals of the experimental group were once subcutaneously injected into the thigh area with a 3-5% aqueous solution of Heraconda at a dose of 10 mg / kg of live weight of the animal. Before suturing the wound, its edges, walls and bottom were smeared with 3-5% ointment based on the fuse of vegetable oils with the addition of dimexide and Heraconda (5% aqueous solution).

 The animals of the control group were not given subcutaneous injections. The edges, walls and bottom of the wound in the control group were powdered with antibiotic powder before suturing.

 After 3-5 days of observation, the animals in the control and experimental groups began to manifest visible differences in the state of the animals.

 In the control group, animals had fever, signs of depression, decreased food irritability, and severe swelling at the surgical site.

In the experimental group, 3-5 days after the surgical intervention, intensive smoothing of signs of wound inflammation was observed, the wounds healed without complications by primary intention. Sutures were removed 7-8 days after surgery. Three animals of the control group with no signs of healing by primary intention (suppuration of wounds, the presence of fever, the body temperature of 41.2 ^{° C,} seams partially removed and the wound healing on the mixed type known and proposed). In this case, the wounds healed on days 13-17 after the operation.

 To implement a method of treating radiation burns, an example of the use of an 8-12% solution of Heraconda for the treatment of radiation injuries is considered.

 Patient Radionova A.S. Born 1925 from. Ds: liposarcoma of the left inguinal region, after excision of the tumor, then dist. therapy for postoperative scar Σ 001 50 g; at a dose of 46 gr. a deep radiation ulcer appeared in the center of the postoperative scar, then another one more medially. Dense infiltration of the surrounding tissues in the left iliac inguinal region and edema in / 2 of the left thigh increased. Treatment with standard methods had no effect.

 The patient was treated with daily dressings with a 10% solution of Heraconda. At the time of starting treatment, the ulcer from the left ileo-inguinal region was 4 x 3.5 cm, the depth was 1.5 cm. The edges and bottom of the ulcer were without signs of granulation and epithelization. The second ulcer is ≈ 1 cm in ⌀ 0.7-1.0 cm deep with fibrin. The surrounding tissue is tightly infiltrated, painful.

 After 30 days of treatment, both ulcers are completely epithelized. Scars are tender, smooth, surrounding tissues are soft, painless, normal color. There is no swelling on the thigh.

 Patient Kurmanova I.M. Born in 1907 with a diagnosis of skin cancer of the left cheek PA Art. histology squamous non-keratinized cancer. Radiation therapy Σ 001 64.85 g was used in combination with tumor cryodestruction. An ulcer was formed at the site of the tumor, 3.0 cm in diameter and 1.2-1.2 cm deep (almost to the zygomatic bone). Treatment with standard methods had no effect.

 The patient was treated with daily dressings with a 10% solution of Heraconda.

 After 25 days, complete gentle epithelialization of the ulcer was observed. The hem is smooth, with a diameter of ≈ 1.0 cm.

Patient Kovrizhnykh M.K. Born 1922 with a diagnosis of colorectal cancer T ₂ N ₀ M ₀ . Radiation therapy was used, after which severe radiation rectitis developed.

 Standard methods had no effect.

 The patient was treated using microclysters with a 10% solution of Heraconda.

 After 20 days, the pain disappeared, bleeding stopped, the condition is good.

Patient Brazhnik A.V. 1925 After the diagnosis of colorectal cancer T ₁ N _o M _o , combined radiation therapy was applied, Σ 001 65 g, after which severe radiation rectitis, radiation ulcer of the rectum developed.

 Treatment with standard methods had no effect.

 The patient was treated using microclysters with a 10% solution of Heraconda. After 33 days, the pain decreased, the ulcer cleared of fibrin plaque.

 The treatment of allergic dermatitis is shown in the following example.

 After a stay in the forest and a short exposure to the sun, a bright hyperemia appeared in open areas of the skin of the face and hands with even, clear edges, swelling of the skin, and a tingling sensation appeared.

Diagnosed with photodermatosis? systemic lupus erythematosus? allergic dermatitis?
The patient was treated with standard methods (anti-burn ointment), pripolfen, suprastin, phencarol, prednisone, adsorption charcoal, hydrocortisone), which did not give an effect.

 The patient was treated by applying topical dressings with a 10% solution of Heraconda. In addition, adsorption charcoal was prescribed to the patient.

 Three days after the start of treatment with Erakond, a superficial ulcer ⌀ 2.0 cm appeared on the right hand. On the 7th day of treatment, the ulcer epithelized, the edema disappeared, and hyperemia decreased. On the 12th day of treatment, hyperemia on both hands completely disappeared, normal skin color was restored, but the skin remained dry and thinned. After applying the usual nourishing cream, dry skin disappeared. On the face of Herakond was used on the 12th day of treatment. On the 5th day of treatment, the swelling on the face disappeared, the skin took the usual color, hyperemia disappeared.

Claims (1)

 The use of an aqueous extract of alfalfa as a means to treat wounds, ulcers, burns.