RU2035167C1 - Method for production of biologicaly-active aqueous solution - Google Patents

Abstract

FIELD: food industry. SUBSTANCE: method involves preparing aqueous solution of honey by the use of distilled water, holding it at minus 20 C until it is freezed throughout its volume, dissolving honey by applying it to the surface of ice atd an ice-honey mass ratio of 4:1 and holding it when cooling until honey crystals are formed through the entire volume of ice. Then resulting honey solution is exposed to gamma-radiation at a dose of 0.1-10 kGR. EFFECT: higher efficiency. 1 tbl

Description

 The invention relates to biotechnology, and in particular to methods for producing a biologically active aqueous solution of honey, and can be used in medicine and veterinary medicine to create drugs for various purposes (hepatoprotection, stabilization of the immune system, antitumor and lipid-lowering effects, etc.), as well as in the food industry to give the products the above properties.

A known method of obtaining a biostimulating agent by dissolving honey in water to a hypertonic concentration [1]
This method does not provide high activity of the target product.

 The aim of the invention is to increase the biological activity of the target product.

This is achieved in that for preparing the aqueous solution using distilled water which was maintained at -20 ^C to ice formation throughout the volume of honey is dissolved by its application to the surface of the ice at a weight ratio of flax and honey 4: 1, and curing under cooling to the formation of honey crystals over the entire volume of ice, after which the resulting honey solution is subjected to gamma radiation at a dose of 0.1-10 kGy.

 Above this range, irradiation is impractical not only because of the increased power consumption, but also a decrease in the activity of the target product. It is possible that the achievement of the purpose of the method occurs due to the ionization of the components of honey that previously blocked its biological activity.

EXAMPLES EXAMPLE 1 The solvent was distilled water is frozen to ice formation at -20 ° ^C. On the surface of the ice layer deposited previously had been stored at room temperature uncrystallized flower honey in a weight ratio of 4: 1 respectively and incubated for 20 days. at -20 ° ^C. During this period occurs honey dissolving in the ice caused teplomassoperedachey, with its subsequent crystallization.

 The crystallized honey solution is subjected to gamma irradiation with a dose of 10 kGy. The obtained target product has the form of a slightly opalescent light yellow liquid with a small amount of sediment.

 The biological activity of the target product is tested by oral administration to white mice 1 drop per individual for 10 days. Then, 80 mg / kg of sleeping pills hexenal is administered intraperitoneally to mice, the neutralization of which occurs only in the liver. The biological activity of the target product is judged by the duration of the hexenal sleep of mice compared to the duration of the hexenal sleep of mice in the following control groups.

 Control 1.

 Mice are protected with a biologically active honey solution of this series, but not exposed to gamma radiation.

 Control 2.

 Mice are protected with the most effective hepatoprotective agents Essential (functional analogue) in a dosage of 80 mg / kg body weight for 4 days before the administration of hexenal (Saratikov A.S. Vengerovsky A.I. Pauls O.V. Sedykh I.M. on toxic liver damage in an experiment. Pharmacology and Toxicology, 1990, N 5, p. 42-45).

 Control 3.

 Hexenal is administered to mice that are not protected by any drugs.

 In the experimental and control groups of mice, 9-12 individuals.

 The test results are shown in table 1.

 The duration of hexenal sleep in each group of mice differed statistically significantly at p 0.05. Therefore, gamma radiation of a honey solution in this example increases its hepatoprotective activity.

 PRI me R 2. A honey solution is obtained, as in example 1, is divided into 5 volumes, four of which are subjected to gamma radiation at doses of 10 and 100 Gy, 5 and 25 kGy.

 The target products are tested as in example 1.

 The test results are shown in table.2.

 As can be seen from the table. 2, the radiation dose significantly affects the activity of the target product. A small dose (10 Gy) does not give a difference between the irradiated and non-irradiated product at the above significance level, and a large irradiation dose (25 kGy) leads to deactivation.

 PRI me R 3. A honey solution is obtained, as in example 1, and is divided into 2 volumes, one of which is subjected to gamma irradiation with a dose of 160 Gy.

The 14-day-old chickens of the White Leghorn breed of Haysex egg line, highly sensitive to the Routh sarcoma virus BH-RSV (RAV-1) serological subgroup A, are injected with suspension of this virus in the wing membranes in the following doses: 100 OD ₅₀ (left wing) and 10 OD ₅₀ (right wing). For 4-6 days. all chickens developed a tumor. The following groups are formed from these chickens (7-12 individuals in each):
chickens are injected with a platinum inhibitor (hereinafter Pt-inhibitor described in: Reile Herta et al. Arch Pham 1991, v.3, N 7, p. 405-409) in a therapeutic dose of 0.1 ml and 0.1 ml of irradiated honey solution;
the chickens are injected with a Pt inhibitor in the same dose and 0.1 ml of non-irradiated honey solution of the same series;
chickens are treated only with a Pt inhibitor at the above dose;
control (chickens do not receive treatment).

 The test results are shown in table.3.

 As can be seen from Table 3, the complex treatment of Routh sarcoma in chickens with a Pt-inhibitor and honey solutions significantly increases the survival rate and shortens the tumor resorption time. At the same time, the irradiated honey solution allows curing of 80% of chickens, while using an unirradiated honey solution, this indicator is 54.4%. Using one Pt inhibitor allows only 16.7% of chickens to be cured. In the control group (untreated), all chickens died after 12 ± 1 days. after infection.

 Using the proposed method significantly increases the biological activity of a solution of honey, which is illustrated by the above examples. In addition, an analysis of the intensity of immunity to ILT viruses (infectious laryngotracheitis) and New Castle disease (La Sota strain), carried out on 120 white leggorn chickens at the age of 18-25 days, showed that the obtained target product positively affects the immunocompetent system in the formation of post-vaccination immunity (table 4).

Claims (1)

METHOD FOR PRODUCING A BIOLOGICALLY ACTIVE AQUEOUS SOLUTION OF HONEY, characterized in that distilled water is used to prepare an aqueous solution, which is kept at a temperature of 20 ^o C until ice forms throughout the volume, honey is dissolved by applying it to the ice surface with a mass ratio of ice to honey 4 1 and keeping under cooling until the formation of honey crystals over the entire volume of ice, after which the resulting honey solution is subjected to gamma radiation at a dose of 01, 10 kGy.

Images