RU2026083C1 - Method of destroying tumoral cells - Google Patents

Abstract

FIELD: medicine. SUBSTANCE: this method prescribes using ultra-dispersed iron particles each measuring 0.2 to 1.0 mcm as tumor-destroying drug and injecting these into tumor in the form of 10-percent suspension based on 8-percent gelatinol in 1.0 kg dosage. EFFECT: promising results.

Description

 The invention relates to the field of treatment of malignant neoplasms, namely to tumor-destroying agents.

Closest to the proposed method is the use of significantly larger ferromagnetic particles (from 5-20 μm and above), which are introduced (or implanted) into the tumor and exposed to the RF field, as a result of which, when heated to a sufficiently high temperature, a tumor-destructive effect is achieved , since tumor cells (in contrast to normal) die at a temperature of 43.5 ^about C.

The main disadvantages of this method are that
1) relatively large particles cannot be injected into tumors of dense consistency (the use of smaller ferromagnetic particles is ineffective because they are poorly heated in the RF field);
2) heating of tumor tissue is uneven, it carries a risk of stimulating tumor growth, as at a temperature 38-41 ^{° C} observed stimulation of tumor cell proliferation;
3) in the case of reaching a temperature critical for the survival of tumor cells, their death occurs and, as a result, "volley" entry into the circulation system of the tumor decay products, which leads to acute intoxication of the body, sometimes incompatible with life.

 The purpose of the invention is the creation of a tumor-destroying agent devoid of the disadvantages of the prototype. This is achieved by the fact that ultrafine particles of metallic iron with a size of 0.2-1 microns are used as a tumor-destroying agent.

Ultrafine particles of metallic iron (used in powder metallurgy) prepared by the method of plasma at 10,000 ^{° C} under argon particulate iron-LE with the original value of 50-100 microns.

 UD iron particles are almost chemically pure metallic iron (iron is mainly in the electrically neutral state). In appearance, the powder of ultrafine iron resembles a powdery, loose mass of odorless gray, attracted by a magnet. In order to prevent oxidation of UD-iron and, as a result, they lose their tumor-destroying effect, the particles are stored in a vacuum or in a dry atmosphere of nitrogen, helium or argon away from magnets, which practically ensures their indefinite preservation. The technique for producing UD-iron provides its initial sterility.

PRI me R 1. A rat, male, weighing 200 g, intramuscularly transfused Usker-256 sarcoma by introducing an inoculum containing an average of 500 thousand tumor cells. Tumor volume is determined by the Ellipse formula after its measurement in three mutually perpendicular planes. Upon reaching a tumor with a size of 6506 mm ³ , ultrafine iron particles of 0.2-1 μm are introduced into the tumor at a dose of 1 g / kg in the form of a 10% suspension prepared on the basis of 8% gelatinol. 3 days after intratumoral administration of particles, the decay and lysis of tumor particles occurs.

 The tumor-forming effect was histologically confirmed; in the preparations, generalized death of tumor cells, abundant infiltration of the area of the decayed tumor with leukocytes and macrophages was noted. After complete regression of the tumor, the animal was observed for 120 days. Relapse of tumor growth was not noted.

 The tumor-destroying effect of ultrafine iron particles is confirmed on a large experimental material - 45 animals.

 In the control series of experiments, animals with a tumor receiving only 8% sterile gelatinol solution died within 2-3 weeks after the onset of the tumor.

Claims (1)

 METHOD OF TUMOR CELL DESTRUCTION by introducing ferromagnetic materials into the tumor and exposure to the RF field, characterized in that, in order to prevent intoxication of the body, ultrafine iron particles of 0.2 - 1.0 μm are introduced into the tumor.