RU2022109075A - METHOD FOR CRYOPRESERVATION OF CELLS, ORGANS, TISSUE AND ORGANISMS - Google Patents

METHOD FOR CRYOPRESERVATION OF CELLS, ORGANS, TISSUE AND ORGANISMS Download PDF

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RU2022109075A
RU2022109075A RU2022109075A RU2022109075A RU2022109075A RU 2022109075 A RU2022109075 A RU 2022109075A RU 2022109075 A RU2022109075 A RU 2022109075A RU 2022109075 A RU2022109075 A RU 2022109075A RU 2022109075 A RU2022109075 A RU 2022109075A
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Russia
Prior art keywords
cryopreservation
organs
cells
organisms
tissue
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RU2022109075A
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Russian (ru)
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RU2804972C2 (en
Inventor
Анатолий Васильевич Урмацких
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Анатолий Васильевич Урмацких
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1. Способ криоконсервации клеток, органов, тканей и организмов, включающий введение криопротектора в биологический объект, охлаждение до температуры кристаллизации (близкой к 0°С), замораживание, дополнительное охлаждение до температуры хранения и последующее хранение замороженного объекта при низких температурах, отличающийся тем, что биологический объект до замораживания насыщают тяжелой водой.1. A method for cryopreservation of cells, organs, tissues and organisms, including the introduction of a cryoprotector into a biological object, cooling to a crystallization temperature (close to 0°C), freezing, additional cooling to storage temperature and subsequent storage of the frozen object at low temperatures, characterized in that that a biological object is saturated with heavy water before freezing. 2. Способ криоконсервации клеток, органов, тканей и организмов по п. 1, отличающийся тем, что биологический объект, животного или человеческого происхождения насыщают тяжелой водой от 0,1 до 20 об.% от общего объема воды в организме.2. The method of cryopreservation of cells, organs, tissues and organisms according to claim 1, characterized in that a biological object, animal or human origin, is saturated with heavy water from 0.1 to 20 vol.% of the total volume of water in the body.
RU2022109075A 2022-04-05 Method for cryopreservation of cells, organs, tissue and organisms RU2804972C2 (en)

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RU2022109075A true RU2022109075A (en) 2023-10-05
RU2804972C2 RU2804972C2 (en) 2023-10-09

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