RU2017129673A - Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции - Google Patents
Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции Download PDFInfo
- Publication number
- RU2017129673A RU2017129673A RU2017129673A RU2017129673A RU2017129673A RU 2017129673 A RU2017129673 A RU 2017129673A RU 2017129673 A RU2017129673 A RU 2017129673A RU 2017129673 A RU2017129673 A RU 2017129673A RU 2017129673 A RU2017129673 A RU 2017129673A
- Authority
- RU
- Russia
- Prior art keywords
- effectiveness
- treatment
- sec
- polymerase chain
- chain reaction
- Prior art date
Links
- 238000003752 polymerase chain reaction Methods 0.000 title claims 3
- 206010024229 Leprosy Diseases 0.000 title claims 2
- 238000000034 method Methods 0.000 title claims 2
- 241000236488 Lepra Species 0.000 title 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- 241000186362 Mycobacterium leprae Species 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 230000001355 anti-mycobacterial effect Effects 0.000 claims 1
- 239000003926 antimycobacterial agent Substances 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 238000000265 homogenisation Methods 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 229920000136 polysorbate Polymers 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 claims 1
- 230000035899 viability Effects 0.000 claims 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (1)
- Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции (ПЦР), основанный на определении жизнеспособности Mycobacterium leprae, включающий применение ОТ-ПЦР с внешними и внутренними праймерами: 5'-GCATGTCTTGTGGTGGAAAGC-3' и 5'-CACCCCACCAACAAGCTGAT-3' и с использованием флуоресцентного зонда (FAM)-5'-CATCCTGCACCGCA-3'-(RTQ1), отличающийся тем, что ДНК/РНК выделяют из биоптатов и скарификатов кожи с предварительной их гомогенизацией, реакцию проводят в «Real time» при режиме амплификации: 95°С - 5 мин, 60°С - 50 сек - 1 цикл; 95°С - 15 сек, 62°С - 40 сек - 40 циклов, в Трис HCl буфере с KCl, MgCl2 твином и глицеролом и при снижении бактериоскопического индекса (БИН) и процента положительных образцов, полученных через 6 и 9 месяцев после начала лечения, определяют эффективность проводимой антимикобактериальной терапии.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2017129673A RU2688156C2 (ru) | 2017-08-21 | 2017-08-21 | Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2017129673A RU2688156C2 (ru) | 2017-08-21 | 2017-08-21 | Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| RU2017129673A3 RU2017129673A3 (ru) | 2019-02-22 |
| RU2017129673A true RU2017129673A (ru) | 2019-02-22 |
| RU2688156C2 RU2688156C2 (ru) | 2019-05-20 |
Family
ID=65479177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2017129673A RU2688156C2 (ru) | 2017-08-21 | 2017-08-21 | Способ оценки эффективности лечения лепры с помощью полимеразной цепной реакции |
Country Status (1)
| Country | Link |
|---|---|
| RU (1) | RU2688156C2 (ru) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2759232C1 (ru) * | 2021-01-20 | 2021-11-11 | Федеральное государственное бюджетное учреждение "Государственный научный центр дерматовенерологии и косметологии" Министерства здравоохранения Российской Федерации | Способ оценки эффективности терапии лепры с использованием полимеразной цепной реакции для амплификации видоспецифичных участков геномов лепры, человека или мыши |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2247374C1 (ru) * | 2003-06-09 | 2005-02-27 | Научно-исследовательский институт по изучению лепры Министерства здравоохранения Российской Федерации | Способ прогнозирования эффективности терапии у больных лепрой |
-
2017
- 2017-08-21 RU RU2017129673A patent/RU2688156C2/ru active
Also Published As
| Publication number | Publication date |
|---|---|
| RU2017129673A3 (ru) | 2019-02-22 |
| RU2688156C2 (ru) | 2019-05-20 |
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