RU2016132731A - Method for rapid diagnosis of nodular cattle dermatitis - Google Patents

Method for rapid diagnosis of nodular cattle dermatitis Download PDF

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Publication number
RU2016132731A
RU2016132731A RU2016132731A RU2016132731A RU2016132731A RU 2016132731 A RU2016132731 A RU 2016132731A RU 2016132731 A RU2016132731 A RU 2016132731A RU 2016132731 A RU2016132731 A RU 2016132731A RU 2016132731 A RU2016132731 A RU 2016132731A
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RU
Russia
Prior art keywords
nodular
rapid diagnosis
pathological material
dermatitis
cattle
Prior art date
Application number
RU2016132731A
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Russian (ru)
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RU2648773C2 (en
Inventor
Олег Юрьевич Черных
Василий Александрович Баннов
Алексей Владимирович Мищенко
Владимир Николаевич Шевкопляс
Роман Анатольевич Кривонос
Андрей Георгиевич Кощаев
Георгий Анастасович Джаилиди
Юрий Андреевич Лысенко
Александр Анатолиевич Лысенко
Светлана Георгиевна Дресвянникова
Альберт Николаевич Чернов
Михаил Геннадиевич Коновалов
Original Assignee
Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина"
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Application filed by Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" filed Critical Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина"
Priority to RU2016132731A priority Critical patent/RU2648773C2/en
Publication of RU2016132731A publication Critical patent/RU2016132731A/en
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Publication of RU2648773C2 publication Critical patent/RU2648773C2/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Claims (1)

Способ экспресс-диагностики нодулярного дерматита КРС, включающий отбор проб патологического материала, экстракцию ДНК вируса инфекционного заболевания из полученных проб, проведение полимеразной цепной реакции (ПЦР) с флуоресцентной детекцией в режиме реального времени, учет результатов анализа, отличающийся тем, что в качестве патологического материала используют пробы из очага инфекционного заболевания нодулярного дерматита крупного рогатого скота, при этом отбор проб патологического материала осуществляют на начальной стадии инфицирования животных и проводят экспресс-диагностику полученного патологического материала в течение суток.A method for the rapid diagnosis of nodular cattle dermatitis, including sampling of pathological material, extraction of infectious disease virus DNA from the obtained samples, polymerase chain reaction (PCR) with fluorescence detection in real time, taking into account the results of the analysis, characterized in that as a pathological material use samples from the focus of an infectious disease of nodular cattle dermatitis, while sampling of pathological material is carried out at the initial stage nfitsirovaniya animals and conduct rapid diagnosis of pathological material obtained during the day.
RU2016132731A 2016-08-08 2016-08-08 Method of express diagnostics of nodular dermatitis in cattle RU2648773C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2016132731A RU2648773C2 (en) 2016-08-08 2016-08-08 Method of express diagnostics of nodular dermatitis in cattle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2016132731A RU2648773C2 (en) 2016-08-08 2016-08-08 Method of express diagnostics of nodular dermatitis in cattle

Publications (2)

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RU2016132731A true RU2016132731A (en) 2018-02-13
RU2648773C2 RU2648773C2 (en) 2018-03-28

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RU2016132731A RU2648773C2 (en) 2016-08-08 2016-08-08 Method of express diagnostics of nodular dermatitis in cattle

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RU (1) RU2648773C2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117987601A (en) * 2024-04-07 2024-05-07 山东农业大学 Detection kit for bovine nodular skin disease virus and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2719719C1 (en) * 2019-10-16 2020-04-22 Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный аграрный университет имени И.Т. Трубилина" Method for detection of dna of nodular dermatitis virus (lsdv) in animal biological material by means of polymerase chain reaction in real time

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2202112C1 (en) * 2001-07-26 2003-04-10 ВНИИ ветеринарной вирусологии и микробиологии Method for detecting specific antibodies to sheep and goat pox viruses due to two- phase inhibition of solid-phase immunoenzymatic assay based upon monoclonal antibodies

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117987601A (en) * 2024-04-07 2024-05-07 山东农业大学 Detection kit for bovine nodular skin disease virus and application thereof

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RU2648773C2 (en) 2018-03-28

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20180809