RU2015140181A - METHOD FOR PRODUCING DNA FROM FISH EGGS - Google Patents

METHOD FOR PRODUCING DNA FROM FISH EGGS Download PDF

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Publication number
RU2015140181A
RU2015140181A RU2015140181A RU2015140181A RU2015140181A RU 2015140181 A RU2015140181 A RU 2015140181A RU 2015140181 A RU2015140181 A RU 2015140181A RU 2015140181 A RU2015140181 A RU 2015140181A RU 2015140181 A RU2015140181 A RU 2015140181A
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RU
Russia
Prior art keywords
dna
fish eggs
producing dna
eggs
sample preparation
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RU2015140181A
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Russian (ru)
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RU2632649C2 (en
Inventor
Елена Викторовна Иванёха
Людмила Николаевна Дума
Александр Витальевич Рекубратский
Original Assignee
Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт пресноводного рыбного хозяйства" ФГБНУ "ВНИИПРХ"
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Application filed by Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт пресноводного рыбного хозяйства" ФГБНУ "ВНИИПРХ" filed Critical Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт пресноводного рыбного хозяйства" ФГБНУ "ВНИИПРХ"
Priority to RU2015140181A priority Critical patent/RU2632649C2/en
Publication of RU2015140181A publication Critical patent/RU2015140181A/en
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Publication of RU2632649C2 publication Critical patent/RU2632649C2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (2)

1. Способ получения ДНК из яйцеклеток рыб, предусматривающий пробоподготовку и выделение ДНК, отличающийся тем, что в процессе пробоподготовки яйцеклетки осеменяют генетически инактивированной спермой для получения гиногенетических эмбрионов, из которых после вылупления выделяют ДНК.1. A method of obtaining DNA from fish eggs, involving sample preparation and DNA isolation, characterized in that during the sample preparation, the eggs are seeded with genetically inactivated sperm to obtain gynogenetic embryos, from which DNA is isolated after hatching. 2. Способ по п. 1, отличающийся тем, что выделение ДНК проводится методом солевой экстракции.2. The method according to p. 1, characterized in that the DNA extraction is carried out by the method of salt extraction.
RU2015140181A 2015-09-21 2015-09-21 Method of obtaining dna from fish ovicell RU2632649C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2015140181A RU2632649C2 (en) 2015-09-21 2015-09-21 Method of obtaining dna from fish ovicell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2015140181A RU2632649C2 (en) 2015-09-21 2015-09-21 Method of obtaining dna from fish ovicell

Publications (2)

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RU2015140181A true RU2015140181A (en) 2017-03-27
RU2632649C2 RU2632649C2 (en) 2017-10-06

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RU2015140181A RU2632649C2 (en) 2015-09-21 2015-09-21 Method of obtaining dna from fish ovicell

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113736852A (en) * 2021-09-07 2021-12-03 武汉奥科鼎盛生物科技有限公司 Fish egg cell lysate and rapid PCR amplification sequencing detection method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2312495C2 (en) * 2005-12-21 2007-12-20 Виктор Павлович Васильев Method for obtaining unisexual female offspring in sturgeon fish

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113736852A (en) * 2021-09-07 2021-12-03 武汉奥科鼎盛生物科技有限公司 Fish egg cell lysate and rapid PCR amplification sequencing detection method

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Publication number Publication date
RU2632649C2 (en) 2017-10-06

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