RU2015121051A - METHOD FOR SELECTING TRICHODERMA STRAINS AND METHOD FOR PRODUCING A BIOLOGICAL PRODUCT BASED ON SUCH STRAINS - Google Patents

METHOD FOR SELECTING TRICHODERMA STRAINS AND METHOD FOR PRODUCING A BIOLOGICAL PRODUCT BASED ON SUCH STRAINS Download PDF

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RU2015121051A
RU2015121051A RU2015121051A RU2015121051A RU2015121051A RU 2015121051 A RU2015121051 A RU 2015121051A RU 2015121051 A RU2015121051 A RU 2015121051A RU 2015121051 A RU2015121051 A RU 2015121051A RU 2015121051 A RU2015121051 A RU 2015121051A
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strains
biological product
trichoderma
fedorov
phytopathogens
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RU2015121051A
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Russian (ru)
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RU2607785C2 (en
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Ольга Анатольевна Лягушина
Андрей Иванович Сидякин
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Филоненко Владимир Александрович
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

1. Способ отбора штаммов гриба Trichoderma для производства биопрепарата для стимуляции роста растений и их защиты от фитопатогенов, отличающийся тем, что для отбора штаммов используют градиент концентраций любого арилметанового красителя из трифенилметанового ряда: бриллиантовый зеленый, генциановый фиолетовый или кристаллический фиолетовый в пределах от 1,0 до 30 мг/л.2. Способ получения биопрепарата для стимуляции роста растений и их защиты от фитопатогенов на основе штаммов Trichoderma по п. 1, отличающийся тем, что биопрепарат получают совместным двухстадийным культивированием указанных штаммов на питательной среде следующего состава (г/дм): KHPO- 0,9, KHPO- 0,6, NHCl - 1,25, NaCl - 0,1, сахароза - 1,0, меласса - 40,0, MgSO- 0,1, раствор микроэлементов по Федорову - 4 см, а затем на среде (г/дм): NHCl - 1,5; KHPO- 2,0, MgSO- 2,0, NaCl - 1,0 CaCl- 10,0 раствор микроэлементов по Федорову - 1,0 см, меласса - 40,0.1. A method of selecting strains of Trichoderma fungus for the production of a biological product to stimulate plant growth and protect them from phytopathogens, characterized in that the selection of strains uses a concentration gradient of any aryl methane dye from the triphenylmethane series: brilliant green, genzian violet or crystalline violet ranging from 1, 0 to 30 mg / l. 2. A method of obtaining a biological product to stimulate plant growth and protect them from phytopathogens based on Trichoderma strains according to claim 1, characterized in that the biological product is obtained by joint two-stage cultivation of these strains on a nutrient medium of the following composition (g / dm): KHPO-0.9, KHPO - 0.6, NHCl - 1.25, NaCl - 0.1, sucrose - 1.0, molasses - 40.0, MgSO-0.1, a trace element solution according to Fedorov - 4 cm, and then on medium (g / dm): NHCl - 1.5; KHPO-2.0, MgSO-2.0, NaCl - 1.0 CaCl - 10.0 trace element solution according to Fedorov - 1.0 cm, molasses - 40.0.

Claims (2)

1. Способ отбора штаммов гриба Trichoderma для производства биопрепарата для стимуляции роста растений и их защиты от фитопатогенов, отличающийся тем, что для отбора штаммов используют градиент концентраций любого арилметанового красителя из трифенилметанового ряда: бриллиантовый зеленый, генциановый фиолетовый или кристаллический фиолетовый в пределах от 1,0 до 30 мг/л.1. A method of selecting strains of Trichoderma fungus for the production of a biological product to stimulate plant growth and protect them from phytopathogens, characterized in that the selection of strains uses a concentration gradient of any aryl methane dye from the triphenylmethane series: brilliant green, genzian violet or crystalline violet ranging from 1, 0 to 30 mg / l. 2. Способ получения биопрепарата для стимуляции роста растений и их защиты от фитопатогенов на основе штаммов Trichoderma по п. 1, отличающийся тем, что биопрепарат получают совместным двухстадийным культивированием указанных штаммов на питательной среде следующего состава (г/дм3): K2HPO4 - 0,9, KH2PO4 - 0,6, NH4Cl - 1,25, NaCl - 0,1, сахароза - 1,0, меласса - 40,0, MgSO4 - 0,1, раствор микроэлементов по Федорову - 4 см3, а затем на среде (г/дм3): NH4Cl - 1,5; KH2PO4 - 2,0, MgSO4 - 2,0, NaCl - 1,0 CaCl2 - 10,0 раствор микроэлементов по Федорову - 1,0 см3, меласса - 40,0. 2. A method of obtaining a biological product to stimulate plant growth and protect them from phytopathogens based on Trichoderma strains according to claim 1, characterized in that the biological product is obtained by joint two-stage cultivation of these strains on a nutrient medium of the following composition (g / dm 3 ): K 2 HPO 4 - 0.9, KH 2 PO 4 - 0.6, NH 4 Cl - 1.25, NaCl - 0.1, sucrose - 1.0, molasses - 40.0, MgSO 4 - 0.1, trace element solution according to Fedorov - 4 cm 3 and then on the medium (g / dm 3 ): NH 4 Cl - 1.5; KH 2 PO 4 - 2.0, MgSO 4 - 2.0, NaCl - 1.0 CaCl 2 - 10.0 trace element solution according to Fedorov - 1.0 cm 3 , molasses - 40.0.
RU2015121051A 2015-06-03 2015-06-03 Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains RU2607785C2 (en)

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RU2015121051A RU2607785C2 (en) 2015-06-03 2015-06-03 Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains

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RU2015121051A RU2607785C2 (en) 2015-06-03 2015-06-03 Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113549557A (en) * 2021-06-25 2021-10-26 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） Trichoderma composite biological agent for preventing and treating blight of casserole watermelon and application thereof
CN114431255A (en) * 2021-10-29 2022-05-06 中国科学院烟台海岸带研究所 Application of trichoderma asperellum and plant growth promoting microbial inoculum

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2654564C1 (en) * 2017-03-10 2018-05-21 Аркадий Пантелеймонович Синицын Strain of trichoderma longibrachiatum tw-14-220 filamentous fungus - producer of cellulases, beta-glucanases and xylanases for feed production and a method for obtaining a feed complex enzyme preparation

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1671684A1 (en) * 1989-06-30 1991-08-23 Научно-исследовательский институт овощного хозяйства Method selection of strain fungi trichaderma - antagonists of phytopathogenic fungi
RU2035145C1 (en) * 1993-01-22 1995-05-20 Анатолий Петрович Третьяков Trichodermine productiuon method
ES2200602B1 (en) * 2000-04-28 2005-04-01 Universidad De Salamanca COMPOSITION THAT INCLUDES FUNDS OF THE GENUINE USEFUL TRICHODERMA AS A BIOLOGICAL CONTROL AGENT AND ITS APPLICATIONS.
CN101637187B (en) * 2008-10-23 2013-06-19 中国农业科学院农业资源与农业区划研究所 Microbial inoculum for preventing and curing fungal diseases of plants
RU2534213C2 (en) * 2012-03-12 2014-11-27 Диана Ирековна Тазетдинова Method of obtaining biological preparation for protection of plants against phytopathogens and nematodes based on genus trichoderma fungus strain and biological preparation obtained thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113549557A (en) * 2021-06-25 2021-10-26 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） Trichoderma composite biological agent for preventing and treating blight of casserole watermelon and application thereof
CN113549557B (en) * 2021-06-25 2023-01-03 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） Trichoderma composite biological agent for preventing and treating blight of casserole watermelon and application thereof
CN114431255A (en) * 2021-10-29 2022-05-06 中国科学院烟台海岸带研究所 Application of trichoderma asperellum and plant growth promoting microbial inoculum

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