RU2013151577A

RU2013151577A - METHOD FOR PRODUCING GUANINE

Info

Publication number: RU2013151577A
Application number: RU2013151577/05A
Authority: RU
Inventors: Марфуга Дюсембаевна Мукатова; Наталья Александровна Киричко; Фарида Хазаралиевна Шахбазова
Priority date: 2013-11-19
Filing date: 2013-11-19
Publication date: 2015-05-27
Also published as: RU2569545C2

Abstract

Способ получения гуанина, включающий снятие гуанина и очистку его от белковых и липидных загрязнений, отличающийся тем, что в качестве сырья используют кожу с чешуей вяленой воблы, снятие гуанина осуществляют замачиванием ее в 10%-ном растворе карбамида, нагревают смесь до 45°C при соотношении 1:10, выдерживают смесь при температуре окружающей среды в течение 60-72 ч, затем очищают гуанин внесением 0,3% фермента трипсина, создают слабо щелочную среду добавлением 0,0125% водного раствора ПАВ, например хозяйственного мыла с pH: 7,8-8, в соотношении 1:1,5, ферментируют смесь при температуре 37°C в течение 24 ч.A method of producing guanine, including the removal of guanine and its purification from protein and lipid contaminants, characterized in that the skin with dried roach scales is used as a raw material, guanine is removed by soaking it in a 10% urea solution, the mixture is heated to 45 ° C at a ratio of 1:10, the mixture is kept at ambient temperature for 60-72 hours, then guanine is purified by adding 0.3% trypsin enzyme, a slightly alkaline environment is created by adding 0.0125% aqueous surfactant solution, for example, laundry soap with pH: 7, 8-8, in the ratio of 1: 1.5, farms Orient the mixture at a temperature of 37 ° C for 24 hours.

Claims

A method of producing guanine, including the removal of guanine and its purification from protein and lipid contaminants, characterized in that the skin with dried roach scales is used as a raw material, guanine is removed by soaking it in a 10% urea solution, the mixture is heated to 45 ° C at a ratio of 1:10, the mixture is kept at ambient temperature for 60-72 hours, then guanine is purified by adding 0.3% trypsin enzyme, a slightly alkaline environment is created by adding 0.0125% aqueous surfactant solution, for example, laundry soap with pH: 7, 8-8, in the ratio of 1: 1.5, farms Orient the mixture at a temperature of 37 ° C for 24 hours.