RU2013138738A - REAGENT, ALLOWING TO INACTIVATE MICRO-ORGANISMS, EXTRACT AND SAVE DNA OF BACTERIA IN A FORM SUITABLE FOR HIGH-EFFICIENT MOLECULAR DIAGNOSTICS - Google Patents

REAGENT, ALLOWING TO INACTIVATE MICRO-ORGANISMS, EXTRACT AND SAVE DNA OF BACTERIA IN A FORM SUITABLE FOR HIGH-EFFICIENT MOLECULAR DIAGNOSTICS Download PDF

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Publication number
RU2013138738A
RU2013138738A RU2013138738/15A RU2013138738A RU2013138738A RU 2013138738 A RU2013138738 A RU 2013138738A RU 2013138738/15 A RU2013138738/15 A RU 2013138738/15A RU 2013138738 A RU2013138738 A RU 2013138738A RU 2013138738 A RU2013138738 A RU 2013138738A
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RU
Russia
Prior art keywords
reagent
biological sample
pcr
organisms
bacteria
Prior art date
Application number
RU2013138738/15A
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Russian (ru)
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RU2574917C2 (en
Inventor
Дмитрий Александрович Варламов
Дмитрий Гарриевич Сочивко
Юлия Сергеевна Аляпкина
Алексей Владимирович Кузубов
Лариса Николаевна Черноусова
Татьяна Владимировна Смирнова
Елена Евгеньевна Ларионова
Софья Николаевна Андреевская
Владимир Георгиевич Нестеренко
Виктор Вениаминович Милитицкий
Анатолий Петрович Суслов
Наталья Юрьевна Макарова
Original Assignee
Общество С Ограниченной Ответственностью "Ниармедик Плюс"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Общество С Ограниченной Ответственностью "Ниармедик Плюс" filed Critical Общество С Ограниченной Ответственностью "Ниармедик Плюс"
Priority to RU2013138738/15A priority Critical patent/RU2574917C2/en
Priority claimed from RU2013138738/15A external-priority patent/RU2574917C2/en
Priority to EA201690347A priority patent/EA032990B1/en
Priority to PCT/RU2014/000616 priority patent/WO2015026268A1/en
Publication of RU2013138738A publication Critical patent/RU2013138738A/en
Application granted granted Critical
Publication of RU2574917C2 publication Critical patent/RU2574917C2/en
Priority to ZA2016/01149A priority patent/ZA201601149B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Abstract

1. Реагент для подготовки биологического образца к in vitro диагностике инфекционного заболевания методом ПЦР, представляющий собой водный раствор, содержащий спирт, щелочь и третичный амин, в концентрациях, обеспечивающих повышение эффективности амплификации не менее чем на 2 цикла.2. Способ подготовки биологического образца к in vitro диагностике инфекционного заболевания методом ПЦР, предусматривающий обработку реагентом, охарактеризованным в п. 1, в течение не менее 1 часа.3. Способ хранения биологического образца, пригодного для in vitro диагностики инфекционного заболевания методом ПЦР, предусматривающий осуществление способа по п. 2.4. Применение способа по п. 2 для хранения биологического образца.1. A reagent for preparing a biological sample for in vitro diagnosis of an infectious disease by PCR, which is an aqueous solution containing alcohol, alkali and tertiary amine, in concentrations that increase the amplification efficiency by at least 2 cycles. 2. A method for preparing a biological sample for in vitro diagnosis of an infectious disease by PCR, comprising treating with the reagent described in paragraph 1 for at least 1 hour. A method for storing a biological sample suitable for in vitro diagnosis of an infectious disease by PCR, the method comprising the method of claim 2.4. The use of the method of claim 2 for storing a biological sample.

Claims (4)

1. Реагент для подготовки биологического образца к in vitro диагностике инфекционного заболевания методом ПЦР, представляющий собой водный раствор, содержащий спирт, щелочь и третичный амин, в концентрациях, обеспечивающих повышение эффективности амплификации не менее чем на 2 цикла.1. A reagent for preparing a biological sample for in vitro diagnosis of an infectious disease by PCR, which is an aqueous solution containing alcohol, alkali and tertiary amine, in concentrations that increase the amplification efficiency of at least 2 cycles. 2. Способ подготовки биологического образца к in vitro диагностике инфекционного заболевания методом ПЦР, предусматривающий обработку реагентом, охарактеризованным в п. 1, в течение не менее 1 часа.2. A method for preparing a biological sample for in vitro diagnosis of an infectious disease by PCR, comprising treating with the reagent described in paragraph 1 for at least 1 hour. 3. Способ хранения биологического образца, пригодного для in vitro диагностики инфекционного заболевания методом ПЦР, предусматривающий осуществление способа по п. 2.3. A method of storing a biological sample suitable for in vitro diagnosis of an infectious disease by PCR, comprising the method of claim 2. 4. Применение способа по п. 2 для хранения биологического образца. 4. The use of the method according to claim 2 for storing a biological sample.
RU2013138738/15A 2013-08-20 2013-08-20 Chemical agent enabling inactivating microorganisms, extracting and preserving bacterial dna in form applicable for high-efficacy molecular diagnostics RU2574917C2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
RU2013138738/15A RU2574917C2 (en) 2013-08-20 Chemical agent enabling inactivating microorganisms, extracting and preserving bacterial dna in form applicable for high-efficacy molecular diagnostics
EA201690347A EA032990B1 (en) 2013-08-20 2014-08-19 Reagent for the treatment and storage of a biological sample
PCT/RU2014/000616 WO2015026268A1 (en) 2013-08-20 2014-08-19 Reagent allowing for microorganism inactivation, extraction and storage of bacterial dna in form suitable for highly effective molecular diagnostics
ZA2016/01149A ZA201601149B (en) 2013-08-20 2016-02-19 Reagent allowing for microorganism inactivation, extraction and storage of bacterial dna in form suitable for highly effective molecular diagnostics

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2013138738/15A RU2574917C2 (en) 2013-08-20 Chemical agent enabling inactivating microorganisms, extracting and preserving bacterial dna in form applicable for high-efficacy molecular diagnostics

Publications (2)

Publication Number Publication Date
RU2013138738A true RU2013138738A (en) 2015-02-27
RU2574917C2 RU2574917C2 (en) 2016-02-10

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Also Published As

Publication number Publication date
WO2015026268A1 (en) 2015-02-26
EA201690347A1 (en) 2016-06-30
ZA201601149B (en) 2017-05-31
EA032990B1 (en) 2019-08-30

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