RU2013134870A - METHOD FOR ISOLATING PROTEINS FROM BONE MARROW CELLS - Google Patents
METHOD FOR ISOLATING PROTEINS FROM BONE MARROW CELLSInfo
- Publication number
- RU2013134870A RU2013134870A RU2013134870/10A RU2013134870A RU2013134870A RU 2013134870 A RU2013134870 A RU 2013134870A RU 2013134870/10 A RU2013134870/10 A RU 2013134870/10A RU 2013134870 A RU2013134870 A RU 2013134870A RU 2013134870 A RU2013134870 A RU 2013134870A
- Authority
- RU
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- Prior art keywords
- solution
- precipitate
- bone marrow
- centrifugation
- rpm
- Prior art date
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- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Способ выделения белков из костного мозга, предусматривающий извлечение клеток костного мозга, их суспендирование, фильтрацию суспензии, центрифугирование фильтрата, отстаивание 12 ч при температуре 4°C, полученный раствор белка подвергают диализу проточной водой в течение 1-2 суток и дистиллированной водой в течение 1 ч, полученный осадок смывают спиртом и эфиром, затем высушивают на часовом стекле, к полученному порошку добавляют 0,9%=ного раствора хлорида натрия, тщательно перемешивают, отличающийся тем, что после центрифугирования осадки вместе с надосадочными жидкостями подвергают гомогенизации с помощью гомогенизатора, затем содержимое центрифужных пробирок фильтруют через капроновые фильтры, белки раствора высаливают с помощью сульфата аммония при нагревании до 60°С и центрифугируют при 2000 об/мин в течение 5 мин, к надосадочной жидкости добавляют 1%=ный раствор трихлоруксусной кислоты, затем также осадок от раствора отделяют центрифугированием при 2000 об/мин в течение 5 мин.A method of isolating proteins from bone marrow, which involves the extraction of bone marrow cells, their suspension, filtering the suspension, centrifuging the filtrate, settling for 12 hours at 4 ° C, the resulting protein solution is subjected to dialysis with running water for 1-2 days and distilled water for 1 h, the precipitate obtained is washed off with alcohol and ether, then dried on a watch glass, 0.9% sodium chloride solution is added to the obtained powder, mixed thoroughly, characterized in that after centrifugation the precipitate and together with the supernatants, homogenize using a homogenizer, then the contents of the centrifuge tubes are filtered through nylon filters, the solution proteins are salted out with ammonium sulfate when heated to 60 ° C and centrifuged at 2000 rpm for 5 minutes, 1 is added to the supernatant % = trichloroacetic acid solution, then the precipitate is also separated from the solution by centrifugation at 2000 rpm for 5 minutes.
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Priority Applications (1)
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RU2013134870/10A RU2553334C2 (en) | 2013-07-23 | 2013-07-23 | Method of extracting proteins from bone marrow cells |
Applications Claiming Priority (1)
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RU2013134870/10A RU2553334C2 (en) | 2013-07-23 | 2013-07-23 | Method of extracting proteins from bone marrow cells |
Publications (2)
Publication Number | Publication Date |
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RU2013134870A true RU2013134870A (en) | 2015-01-27 |
RU2553334C2 RU2553334C2 (en) | 2015-06-10 |
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RU2013134870/10A RU2553334C2 (en) | 2013-07-23 | 2013-07-23 | Method of extracting proteins from bone marrow cells |
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RU (1) | RU2553334C2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2726615C1 (en) * | 2019-09-16 | 2020-07-15 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Дальневосточный государственный аграрный университет" (ФГБОУ ВО Дальневосточный ГАУ) | Method for recovering proteins from bone marrow of animals |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
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RU2041716C1 (en) * | 1993-01-05 | 1995-08-20 | Рэм Викторович Петров | Method for obtaining myelopid |
RU2298939C2 (en) * | 2005-06-27 | 2007-05-20 | Федеральное государственное образовательное учреждение высшего профессионального образования Дальневосточный государственный аграрный университет | Method for protein isolation from marrow cells |
UA40805U (en) * | 2008-11-26 | 2009-04-27 | Национальный Университет Биоресурсов И Природопользования Украины | method for intravital obtaoining of stem cells of animal marrow |
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RU2553334C2 (en) | 2015-06-10 |
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MM4A | The patent is invalid due to non-payment of fees |
Effective date: 20150726 |