RU2013131303A - METHOD FOR PRODUCING BACTERIOLOGICALLY CLEAN CULTURES OF MARINE MICROALGAE - Google Patents

METHOD FOR PRODUCING BACTERIOLOGICALLY CLEAN CULTURES OF MARINE MICROALGAE Download PDF

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RU2013131303A
RU2013131303A RU2013131303/10A RU2013131303A RU2013131303A RU 2013131303 A RU2013131303 A RU 2013131303A RU 2013131303/10 A RU2013131303/10 A RU 2013131303/10A RU 2013131303 A RU2013131303 A RU 2013131303A RU 2013131303 A RU2013131303 A RU 2013131303A
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producing
cultures
fungicide
antibiotics
amount
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RU2013131303/10A
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Russian (ru)
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RU2542481C2 (en
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Дмитрий Федорович Афанасьев
Марина Александровна Сазыкина
Игорь Евгеньевич Цыбульский
Ирина Григорьевна Корпакова
Марина Александровна Морозова
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Федеральное Государственное унитарное предприятие Азовский научно-исследовательский институт рыбного хозяйства
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Priority to RU2013131303/10A priority Critical patent/RU2542481C2/en
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Abstract

Способ получения бактериологически чистых культур морских микроводорослей, включающий химическую стерилизацию культур микроводорослей путем обработки в растворе стерильной морской воды, содержащей 0.1% фенола и 1.0% этилового спирта, отличающийся тем, что дополнительно проводят облучение ультрафиолетовым светом в течение 9-10 мин, а затем обработку антибиотиками и фунгицидом, причем в качестве антибиотиков используют левомицитин и ампициллин в количестве по 50 мкг/мл каждого, а в качестве фунгицида используют нистатин в количестве 25 мкг/мл.A method of producing bacteriologically pure cultures of marine microalgae, including chemical sterilization of microalgae cultures by treatment in a solution of sterile sea water containing 0.1% phenol and 1.0% ethyl alcohol, characterized in that it is additionally irradiated with ultraviolet light for 9-10 minutes, and then processing antibiotics and a fungicide, moreover, as antibiotics use levomycetin and ampicillin in an amount of 50 μg / ml each, and as a fungicide use nystatin in an amount of 25 μg / ml .

Claims (1)

Способ получения бактериологически чистых культур морских микроводорослей, включающий химическую стерилизацию культур микроводорослей путем обработки в растворе стерильной морской воды, содержащей 0.1% фенола и 1.0% этилового спирта, отличающийся тем, что дополнительно проводят облучение ультрафиолетовым светом в течение 9-10 мин, а затем обработку антибиотиками и фунгицидом, причем в качестве антибиотиков используют левомицитин и ампициллин в количестве по 50 мкг/мл каждого, а в качестве фунгицида используют нистатин в количестве 25 мкг/мл. A method of producing bacteriologically pure cultures of marine microalgae, including chemical sterilization of microalgae cultures by treatment in a solution of sterile sea water containing 0.1% phenol and 1.0% ethyl alcohol, characterized in that it is additionally irradiated with ultraviolet light for 9-10 minutes, and then processing antibiotics and a fungicide, moreover, as antibiotics use levomycetin and ampicillin in an amount of 50 μg / ml each, and as a fungicide use nystatin in an amount of 25 μg / ml .
RU2013131303/10A 2013-07-08 2013-07-08 Method of production of bacteriologically pure cultures of marine blue-green microalgae RU2542481C2 (en)

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RU2013131303/10A RU2542481C2 (en) 2013-07-08 2013-07-08 Method of production of bacteriologically pure cultures of marine blue-green microalgae

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RU2013131303/10A RU2542481C2 (en) 2013-07-08 2013-07-08 Method of production of bacteriologically pure cultures of marine blue-green microalgae

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114032202A (en) * 2021-12-15 2022-02-11 中国热带农业科学院热带生物技术研究所 Method for screening marine microalgae symbiotic bacteria
CN114249500A (en) * 2021-12-24 2022-03-29 天津大学 Method for purifying high-concentration antibiotic wastewater by re-culturing microalgae through ultraviolet pretreatment

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA009596B1 (en) * 2002-05-13 2008-02-28 Гринфьюел Текнолоджиз Корпорейшн Photobioreactor and process for biomass production and mitigation of pollutants in flue gases
ES2308912B2 (en) * 2007-01-16 2009-09-16 Bernard A.J. Stroiazzo-Mougin ACCELERATED PROCEDURE OF ENERGETIC CONVERSION OF CARBON DIOXIDE.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114032202A (en) * 2021-12-15 2022-02-11 中国热带农业科学院热带生物技术研究所 Method for screening marine microalgae symbiotic bacteria
CN114249500A (en) * 2021-12-24 2022-03-29 天津大学 Method for purifying high-concentration antibiotic wastewater by re-culturing microalgae through ultraviolet pretreatment

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Effective date: 20160709