RU2013125414A - METHOD FOR REDUCING LOW-TEMPERATURE JUMPING OF CRYOPROTECTORS SOLUTIONS - Google Patents

METHOD FOR REDUCING LOW-TEMPERATURE JUMPING OF CRYOPROTECTORS SOLUTIONS Download PDF

Info

Publication number
RU2013125414A
RU2013125414A RU2013125414/13A RU2013125414A RU2013125414A RU 2013125414 A RU2013125414 A RU 2013125414A RU 2013125414/13 A RU2013125414/13 A RU 2013125414/13A RU 2013125414 A RU2013125414 A RU 2013125414A RU 2013125414 A RU2013125414 A RU 2013125414A
Authority
RU
Russia
Prior art keywords
solutions
cryoprotectors
reducing low
cryoprotectant
freezing
Prior art date
Application number
RU2013125414/13A
Other languages
Russian (ru)
Other versions
RU2540598C2 (en
Inventor
Алексей Алексеевич Андреев
Диана Габдельфартовна Садикова
Елена Николаевна Пономарева
Александра Андриановна Красильникова
Мария Михайловна Белая
Original Assignee
Федеральное государственное бюджетное образовательное учреждение Высшего профессионального образования Астраханский государственный технический университет (ФГБОУ ВПО АГТУ)
Федеральное государственное бюджетное учреждение науки Южный научный центр Российской академии наук (ЮНЦ РАН)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Федеральное государственное бюджетное образовательное учреждение Высшего профессионального образования Астраханский государственный технический университет (ФГБОУ ВПО АГТУ), Федеральное государственное бюджетное учреждение науки Южный научный центр Российской академии наук (ЮНЦ РАН) filed Critical Федеральное государственное бюджетное образовательное учреждение Высшего профессионального образования Астраханский государственный технический университет (ФГБОУ ВПО АГТУ)
Priority to RU2013125414/13A priority Critical patent/RU2540598C2/en
Publication of RU2013125414A publication Critical patent/RU2013125414A/en
Application granted granted Critical
Publication of RU2540598C2 publication Critical patent/RU2540598C2/en

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Способ снижения низкотемпературного скачка растворов криопротекторов, включающий замораживание криораствора с биологическим материалом в жидком азоте, отличающийся тем, что до операции замораживания раствора криопротекторов с клетками живых организмов осуществляют дистанционное воздействие на замораживаемый раствор ультразвуковым излучением частотой 0,50-10 МГц.A method of reducing the low-temperature jump of cryoprotectant solutions, including freezing a cryoprotectant with biological material in liquid nitrogen, characterized in that prior to the operation of freezing a solution of cryoprotectants with cells of living organisms, a remote exposure of the frozen solution to ultrasound radiation of 0.50-10 MHz is carried out.

Claims (1)

Способ снижения низкотемпературного скачка растворов криопротекторов, включающий замораживание криораствора с биологическим материалом в жидком азоте, отличающийся тем, что до операции замораживания раствора криопротекторов с клетками живых организмов осуществляют дистанционное воздействие на замораживаемый раствор ультразвуковым излучением частотой 0,50-10 МГц. A method of reducing the low-temperature jump of cryoprotectant solutions, including freezing a cryoprotectant with biological material in liquid nitrogen, characterized in that prior to the operation of freezing a solution of cryoprotectants with cells of living organisms, a remote exposure of the frozen solution to ultrasound radiation of 0.50-10 MHz is carried out.
RU2013125414/13A 2013-05-31 2013-05-31 Method of reducing low-temperature jump of cryoprotectant solutions RU2540598C2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU2013125414/13A RU2540598C2 (en) 2013-05-31 2013-05-31 Method of reducing low-temperature jump of cryoprotectant solutions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2013125414/13A RU2540598C2 (en) 2013-05-31 2013-05-31 Method of reducing low-temperature jump of cryoprotectant solutions

Publications (2)

Publication Number Publication Date
RU2013125414A true RU2013125414A (en) 2014-12-10
RU2540598C2 RU2540598C2 (en) 2015-02-10

Family

ID=53287249

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2013125414/13A RU2540598C2 (en) 2013-05-31 2013-05-31 Method of reducing low-temperature jump of cryoprotectant solutions

Country Status (1)

Country Link
RU (1) RU2540598C2 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1551315A1 (en) * 1988-06-27 1990-03-23 Украинский научно-исследовательский институт птицеводства Method of freezing embrios of hens
US6521402B1 (en) * 2000-06-14 2003-02-18 The Texas A&M University System Cryopreservation of tissues for use in nuclear transfer
RU2317703C1 (en) * 2006-06-02 2008-02-27 Федеральное государственное унитарное предприятие "Всероссийский научно-исследовательский институт пресноводного рыбного хозяйства" (ФГУП "ВНИИПРХ") Method for cryogenic preservation of sturgeon fish semen
UA18979U (en) * 2006-07-03 2006-11-15 Vinnytsia State Agrarian Unive Device to cover feeds with film

Also Published As

Publication number Publication date
RU2540598C2 (en) 2015-02-10

Similar Documents

Publication Publication Date Title
WO2015199489A3 (en) Heterocyclic compound and organic light emitting device using same
WO2015023560A3 (en) Vessels and methods for cryopreservation
PH12017501559A1 (en) Uses of insecticidal protein
JP2011184444A5 (en)
CL2013002689A1 (en) Methods to increase the yield of plants of interest, by applying the trinexapac-ethyl plant growth regulator and the azoxystrobin fungicide.
SG196722A1 (en) Thawing vessel for biological products
ES2440666B1 (en) SYSTEM AND METHOD TO SUPERVISE THE HEALTH OF HELIOSTATES
RU2013125414A (en) METHOD FOR REDUCING LOW-TEMPERATURE JUMPING OF CRYOPROTECTORS SOLUTIONS
RU2013138082A (en) METHOD FOR PRODUCING A PRODUCT FOR PROCESSING FRUIT AND VEGETABLE PRODUCTS
CL2012002633A1 (en) Exudation and / or drip irrigation device applicable to pots and outdoor plantations on land comprising a deposit introduced into the mass of land in a pot, superiorly provided with means for loading water and means for defining the shape of the tank and internally of means for the continuous flow of water from micro-drops to the land mass.
ES2663800T3 (en) Generation of glucosamine from plant material
TWD168034S (en) Freeze storage container
RU2013149189A (en) METHOD FOR INCREASING SOIL FERTILITY OF AGRICULTURAL LANDS
RU2013108726A (en) METHOD FOR STORING AGRICULTURAL PRODUCTS
PH12014502844B1 (en) Method for producing concentrated aqueous solution of organic compound
RU2011101134A (en) METHOD OF DESTRUCTION OF AGRICULTURAL SOSNOVSKY GROWTH
CL2016001035A1 (en) Procedure to improve seed storage capacity
ES2525893B1 (en) Organic and biological regenerator to reduce the infectious potential of a soil, procedure for obtaining it and use
CN204969120U (en) Embryo culture ware unfreezes
RU2013109820A (en) METHOD FOR INCREASING THE CONTENT OF HUMUS SOIL WITH APPLICATION OF STRAINS OF STUNBUS BACTERIA YAKUTSKY No. 2 ALFUTERS
UA95947U (en) A method for erythrocytes cryopreservation
IN2014DN07033A (en)
PL409382A1 (en) Method for transformation of dry digestate into fluid organic fertilizer and the fluid organic fertilizer
贾正英 Life is great if we have friends
Maxwell Cornstalk Strip Grazing Demonstration with Beef Cows