RU2012143805A - GENETIC DESIGN CONTAINING HGDNF UNDER CONTROL OF TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED EXPRESSION OF A NEUROTROPHIC FACTOR AS IN CELLS, AND DIRECTLY ON THE ORGANISM - Google Patents

GENETIC DESIGN CONTAINING HGDNF UNDER CONTROL OF TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED EXPRESSION OF A NEUROTROPHIC FACTOR AS IN CELLS, AND DIRECTLY ON THE ORGANISM Download PDF

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RU2012143805A
RU2012143805A RU2012143805/10A RU2012143805A RU2012143805A RU 2012143805 A RU2012143805 A RU 2012143805A RU 2012143805/10 A RU2012143805/10 A RU 2012143805/10A RU 2012143805 A RU2012143805 A RU 2012143805A RU 2012143805 A RU2012143805 A RU 2012143805A
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promoter
temperature
gene
heat shock
cells
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RU2012143805/10A
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Russian (ru)
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RU2527169C2 (en
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Галина Валериевна Павлова
Александр Владимирович Ревищин
Екатерина Юрьевна Рыбалкина
Илья Борисович Мерцалов
Надежда Николаевна Куст
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Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН)
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Abstract

Генетическая конструкция, экспрессирующая GDNF в клетках и тканях млекопитающих (в том числе и человека) при повышении температуры, сконструированная на основе векторной плазмиды pEGFP-N1 с геном устойчивости к неомицину, содержащая фрагмент ДНК, кодирующий регуляторную последовательность промотора, с элементами теплового шока HSE более 4, промотор гена белка теплового шока hsp70 Drosophila melanogaster, ген нейротрофического фактора GDNF человека, ген зеленого флуоресцентного белка (GFP), находящийся под контролем данного промотора, при этом промотор способен активироваться под действием температуры теплового шока млекопитающих или токсическим воздействием.Genetic construct expressing GDNF in mammalian (including human) cells and tissues at an increase in temperature, constructed on the basis of the vector plasmid pEGFP-N1 with the neomycin resistance gene, containing a DNA fragment encoding the regulatory sequence of the promoter, with HSE elements of heat shock more 4, the promoter of the heat shock shock protein gene hsp70 Drosophila melanogaster, the gene for human neurotrophic factor GDNF, the gene for green fluorescent protein (GFP) under the control of this promoter, while the promoter is capable of activated by the temperature of heat shock of mammals or toxic effects.

Claims (1)

Генетическая конструкция, экспрессирующая GDNF в клетках и тканях млекопитающих (в том числе и человека) при повышении температуры, сконструированная на основе векторной плазмиды pEGFP-N1 с геном устойчивости к неомицину, содержащая фрагмент ДНК, кодирующий регуляторную последовательность промотора, с элементами теплового шока HSE более 4, промотор гена белка теплового шока hsp70 Drosophila melanogaster, ген нейротрофического фактора GDNF человека, ген зеленого флуоресцентного белка (GFP), находящийся под контролем данного промотора, при этом промотор способен активироваться под действием температуры теплового шока млекопитающих или токсическим воздействием. Genetic construct expressing GDNF in mammalian (including human) cells and tissues at an increase in temperature, constructed on the basis of the vector plasmid pEGFP-N1 with the neomycin resistance gene, containing a DNA fragment encoding the regulatory sequence of the promoter, with HSE elements of heat shock more 4, the promoter of the heat shock shock protein gene hsp70 Drosophila melanogaster, the gene for human neurotrophic factor GDNF, the gene for green fluorescent protein (GFP) under the control of this promoter, while the promoter is capable of activated by the temperature of heat shock of mammals or toxic effects.
RU2012143805/10A 2012-10-15 2012-10-15 GENETIC CONSTRUCT CONTAINING hGDNF CONTROLLED BY TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED NEUROTROPHIC FACTOR EXPRESSION IN MAMMALIAN CELLS AND BODIES DIRECTLY RU2527169C2 (en)

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RU2012143805/10A RU2527169C2 (en) 2012-10-15 2012-10-15 GENETIC CONSTRUCT CONTAINING hGDNF CONTROLLED BY TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED NEUROTROPHIC FACTOR EXPRESSION IN MAMMALIAN CELLS AND BODIES DIRECTLY

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RU2012143805/10A RU2527169C2 (en) 2012-10-15 2012-10-15 GENETIC CONSTRUCT CONTAINING hGDNF CONTROLLED BY TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED NEUROTROPHIC FACTOR EXPRESSION IN MAMMALIAN CELLS AND BODIES DIRECTLY

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EP1879622A2 (en) * 2005-05-03 2008-01-23 Veterinärmedizinische Universität Wien Permeable capsules
FI20070808A0 (en) * 2007-10-25 2007-10-25 Mart Saarma Split variants of GDNF and uses thereof
RU2431669C2 (en) * 2009-06-23 2011-10-20 Государственное образовательное учреждение высшего профессионального образования "Казанский государственный университет им. В.И. Ульянова-Ленина" Method for producing preparation of genetically modified cells

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