RU2012119658A - RECOMBINANT PLASMAID DNA pCI-UB-POLYEPI, CONTAINING EPITOPS OF TUMOR-ASSOCIATED ANTIGENS FOR COLORECTAL CANCER, AND METHOD OF ITS APPLICATION FOR STIMULATION OF SPECIFIC ANTIVECIOLOMATIS - Google Patents

RECOMBINANT PLASMAID DNA pCI-UB-POLYEPI, CONTAINING EPITOPS OF TUMOR-ASSOCIATED ANTIGENS FOR COLORECTAL CANCER, AND METHOD OF ITS APPLICATION FOR STIMULATION OF SPECIFIC ANTIVECIOLOMATIS Download PDF

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RU2012119658A
RU2012119658A RU2012119658/10A RU2012119658A RU2012119658A RU 2012119658 A RU2012119658 A RU 2012119658A RU 2012119658/10 A RU2012119658/10 A RU 2012119658/10A RU 2012119658 A RU2012119658 A RU 2012119658A RU 2012119658 A RU2012119658 A RU 2012119658A
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Russia
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adriw
aayary
polyepi
pci
ary
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RU2012119658/10A
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Russian (ru)
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RU2507265C2 (en
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Сергей Витальевич Сенников
Максим Леонидович Филиппенко
Василий Васильевич Курилин
Екатерина Владимировна Куликова
Евгений Александрович Храпов
Юлия Александровна Шевченко
Роман Викторович Шорохов
Владимир Константинович Якушенко
Андрей Викторович Соколов
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Федеральное государственное бюджетное учреждение "Научно-исследовательский институт клинической иммунологии" Сибирского отделения Российской академии медицинских наук (ФГБУ "НИИКИ" СОРАМН)
Федеральное государственное бюджетное учреждение "Институт химической биологии и фундаментальной медицины" Сибирского отделения Российской академии наук (ФГБУ "ИХБФМ" СО РАН)
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Priority to RU2012119658/10A priority Critical patent/RU2507265C2/en
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

1. Рекомбинантная плазмидная ДНК pCI-UB-POLYEPI, содержит 11 эпитопов опухоль-ассоциированных антигенов колоректального рака, имеет размер 6 355 п.н. и экспрессирует следующую аминокислотную последовательность:DYKDDDDK-LLGVGTFVV-ADRIW-GLKAGVIAV-AAYARY-VLAFGLLLA-ADRIW-YQLDPKFITSI-AAYARY-IMIGVLVGV-ADRIW-YLSGADLNL-AAYARY-CGIQNSVSA-AAYARY-LLLLTVLTV-ADRIW-QYIKANSKFIGITEL-ANIY-SIINFEKL-ARY-SASFDGWATVSVIAL-ARY-SERVRTYWIIIELKHKARE-ARY-IQNDTGFYTLHVIKSDLVNEEрекомбинантная плазмида pCI-UB-POLYEPI содержит CMV энхансер (5251-5910 п.н.), CMV промотор (5919-6001), Т7 промотор (6317-6336 п.н.), убиквитин (1-229), так же содержит уникальные сайты рестрикции EcoRI (233), XbaI(891), NotI (908), BamHI (3164), NdeI (5638), SacI (5980), XhoI (6342).2. Способ генерации антиген-специфических противоопухолевых цитотоксических клеток, заключающийся в совместном культивировании мононуклеарных клеток (МНК) периферической крови больных колоректальным раком с дендритными клетками (ДК), активированными опухолевыми антигенами, отличающийся тем, что для культивирования используют зрелые ДК, полученные добавлением к незрелым ДК провоспалительного цитокина ФНО-α, активированные путем трансфекции в них рекомбинантной плазмидной ДНК pCI-UB-POLYEPI, заявленной в п.1.1. Recombinant plasmid DNA pCI-UB-POLYEPI, contains 11 epitopes of tumor-associated antigens of colorectal cancer, has a size of 6 355 bp and expresses the following amino acid sequence: DYKDDDDK-LLGVGTFVV-ADRIW-GLKAGVIAV-AAYARY-VLAFGLLLA-ADRIW-YQLDPKFITSI-AAYARY-IMIGVLVGV-ADRIW-YLSGADLNL-AAYARY-CGIQNSVSA-AAYARY-LLLLTVLTV-ADRIW-QYIKANSKFIGITEL-ANIY-SIINFEKL-ARY-SASFDGWATVSVIAL- ARY-SERVRTYWIIIELKHKARE-ARY-IQNDTGFYTLHVIKSDLVNEE recombinant plasmid pCI-UB-POLYEPI contains a CMV enhancer (5251-5910 bp), CMV promoter (5919-6001), T7 promoter (6317n.p. 1bp. 229), also contains unique restriction sites EcoRI (233), XbaI (891), NotI (908), BamHI (3164), NdeI (5638), SacI (5980), XhoI (6342) .2. A method for generating antigen-specific antitumor cytotoxic cells, which consists in co-culturing mononuclear cells (MNCs) of peripheral blood of patients with colorectal cancer with dendritic cells (DCs) activated by tumor antigens, characterized in that mature DCs obtained by adding proinflammatory DCs to immature DC are used for cultivation TNF-α cytokine activated by transfection of pCI-UB-POLYEPI recombinant plasmid DNA therein as claimed in claim 1.

Claims (2)

1. Рекомбинантная плазмидная ДНК pCI-UB-POLYEPI, содержит 11 эпитопов опухоль-ассоциированных антигенов колоректального рака, имеет размер 6 355 п.н. и экспрессирует следующую аминокислотную последовательность:1. Recombinant plasmid DNA pCI-UB-POLYEPI, contains 11 epitopes of tumor-associated antigens of colorectal cancer, has a size of 6 355 bp and expresses the following amino acid sequence: DYKDDDDK-LLGVGTFVV-ADRIW-GLKAGVIAV-AAYARY-VLAFGLLLA-ADRIW-DYKDDDDK-LLGVGTFVV-ADRIW-GLKAGVIAV-AAYARY-VLAFGLLLA-ADRIW- YQLDPKFITSI-YQLDPKFITSI- AAYARY-IMIGVLVGV-ADRIW-YLSGADLNL-AAYARY-CGIQNSVSA-AAYARY-AAYARY-IMIGVLVGV-ADRIW-YLSGADLNL-AAYARY-CGIQNSVSA-AAYARY- LLLLTVLTV-ADRIW-QYIKANSKFIGITEL-ANIY-SIINFEKL-ARY-LLLLTVLTV-ADRIW-QYIKANSKFIGITEL-ANIY-SIINFEKL-ARY- SASFDGWATVSVIAL-ARY-SERVRTYWIIIELKHKARE-ARY-SASFDGWATVSVIAL-ARY-SERVRTYWIIIELKHKARE-ARY- IQNDTGFYTLHVIKSDLVNEEIQNDTGFYTLHVIKSDLVNEE рекомбинантная плазмида pCI-UB-POLYEPI содержит CMV энхансер (5251-5910 п.н.), CMV промотор (5919-6001), Т7 промотор (6317-6336 п.н.), убиквитин (1-229), так же содержит уникальные сайты рестрикции EcoRI (233), XbaI(891), NotI (908), BamHI (3164), NdeI (5638), SacI (5980), XhoI (6342).the recombinant plasmid pCI-UB-POLYEPI contains a CMV enhancer (5251-5910 bp), a CMV promoter (5919-6001), a T7 promoter (6317-6336 bp), ubiquitin (1-229), also contains unique restriction sites EcoRI (233), XbaI (891), NotI (908), BamHI (3164), NdeI (5638), SacI (5980), XhoI (6342). 2. Способ генерации антиген-специфических противоопухолевых цитотоксических клеток, заключающийся в совместном культивировании мононуклеарных клеток (МНК) периферической крови больных колоректальным раком с дендритными клетками (ДК), активированными опухолевыми антигенами, отличающийся тем, что для культивирования используют зрелые ДК, полученные добавлением к незрелым ДК провоспалительного цитокина ФНО-α, активированные путем трансфекции в них рекомбинантной плазмидной ДНК pCI-UB-POLYEPI, заявленной в п.1. 2. A method for generating antigen-specific antitumor cytotoxic cells, which consists in co-culturing mononuclear cells (MNCs) of peripheral blood of patients with colorectal cancer with dendritic cells (DC) activated by tumor antigens, characterized in that mature DCs obtained by adding to immature are used for cultivation DC pro-inflammatory cytokine TNF-α activated by transfection of recombinant plasmid DNA pCI-UB-POLYEPI, as claimed in claim 1.
RU2012119658/10A 2012-05-12 2012-05-12 RECOMBINANT PLASMID DNA pCI-UB-POLYEPI CONTAINING EPITOPES OF TUMOUR-ASSOCIATED ANTIGENS FOR COLORECTAL CANCER AND METHOD OF ITS APPLICATION FOR STIMULATION OF SPECIFIC ANTITUMOUR IMMUNE RESPONSE AGAINST COLORECTAL CANCER CELLS RU2507265C2 (en)

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RU2725493C2 (en) * 2018-12-28 2020-07-02 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) System for stable expression of tumor-associated antigens based on lentiviral vector

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RU2684235C2 (en) * 2016-11-29 2019-04-04 Федеральное государственное бюджетное научное учреждение "Научно-исследовательский институт фундаментальной и клинической иммунологии" (НИИФКИ) Polyepitope anti-tumour vaccine structure containing epitopes of tumor-associated antigens, pharmaceutical composition and its application for stimulating specific anti-tumor immune response

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ITRM20050064A1 (en) * 2005-02-16 2006-08-17 Univ Siena POLIEPITOPIC PEPTIDE DERIVED FROM TIMIDILATO SINTETASI WITH IMMUNOLOGICAL AND ANTITUMORAL ACTIVITY AND RELATIVE PHARMACEUTICAL COMPOSITIONS.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2725493C2 (en) * 2018-12-28 2020-07-02 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) System for stable expression of tumor-associated antigens based on lentiviral vector

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