RU2011149592A - METHOD FOR PRODUCING POLYMIXIN B PHARMACEUTICAL SUBSTANCE - Google Patents
METHOD FOR PRODUCING POLYMIXIN B PHARMACEUTICAL SUBSTANCE Download PDFInfo
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- RU2011149592A RU2011149592A RU2011149592/02A RU2011149592A RU2011149592A RU 2011149592 A RU2011149592 A RU 2011149592A RU 2011149592/02 A RU2011149592/02 A RU 2011149592/02A RU 2011149592 A RU2011149592 A RU 2011149592A RU 2011149592 A RU2011149592 A RU 2011149592A
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- polymyxin
- solution
- producing
- exchange resin
- sulfate
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Abstract
1. Способ получения полимиксина В, продуцируемого спорообразующими бактериями Bacillus polymyxa путем очистки нативного раствора, получаемого после отделения биомассы от культуральной жидкости, с использованием неионогенных адсорбентов полистирольного типа с определенными поверхностными параметрами, с элюированием полимиксина B, осаждением полимиксина B основания с последующим переводом его в раствор полимиксина B сульфат с его последующей сушкой, отличающийся тем, что нативный раствор полимиксина B очищают с использованием макропористого сильнокислотного катионита, с последующей обработкой раствора полимиксина B сульфата перед сушкой ионообменной смолой.2. Способ получения полимиксина B по п.1, отличающийся тем, что в качестве макропористого сильнокислотного катионита используется Dowex-MSC-1.3. Способ получения полимиксина B по п.1, отличающийся тем, что в качестве ионообменной смолы для обработки финишного раствора полимиксина B сульфата используется КУ-2-20.1. A method of producing polymyxin B produced by the spore-forming bacteria Bacillus polymyxa by purification of the native solution obtained after separation of biomass from the culture fluid using non-ionic polystyrene adsorbents with certain surface parameters, with elution of polymyxin B, precipitation of polymyxin B base, followed by its transfer to polymyxin B sulfate solution with its subsequent drying, characterized in that the native polymyxin B solution is purified using macroporous c hydrochloric acid cation exchange resin, followed by treatment with a solution of polymyxin B sulfate before drying with an ion exchange resin. 2. The method of producing polymyxin B according to claim 1, characterized in that Dowex-MSC-1.3 is used as a macroporous strongly acidic cation exchanger. The method of producing polymyxin B according to claim 1, characterized in that KU-2-20 is used as an ion exchange resin for processing the final solution of polymyxin B sulfate.
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
Publications (2)
Publication Number | Publication Date |
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RU2011149592A true RU2011149592A (en) | 2013-06-20 |
RU2492180C2 RU2492180C2 (en) | 2013-09-10 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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RU2011149592/10A RU2492180C2 (en) | 2011-12-07 | 2011-12-07 | Method of producing pharmaceutical substance polymyxin b |
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RU (1) | RU2492180C2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018103758A1 (en) * | 2016-12-09 | 2018-06-14 | 上海医药工业研究院 | High-titer polymyxin e2 sodium methanesulfonate |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108977482B (en) * | 2018-08-21 | 2020-11-24 | 浙江孚诺医药股份有限公司 | Preparation method of polymyxin B sulfate |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB782926A (en) * | 1955-01-06 | 1957-09-18 | Distillers Co Yeast Ltd | Purification of polymyxin |
SU111195A1 (en) * | 1957-06-26 | 1957-11-30 | С.М. Мамиофе | Method for producing polymyxin antibiotic |
GB991602A (en) * | 1961-03-10 | 1965-05-12 | Wellcome Found | Antibiotics and their purification |
SK287315B6 (en) * | 2006-06-02 | 2010-06-07 | Biotika, A. S. | A method for polymyxin B isolation from fermented soil |
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2011
- 2011-12-07 RU RU2011149592/10A patent/RU2492180C2/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018103758A1 (en) * | 2016-12-09 | 2018-06-14 | 上海医药工业研究院 | High-titer polymyxin e2 sodium methanesulfonate |
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RU2492180C2 (en) | 2013-09-10 |
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