RU2011133912A - RESISTANCE OF PLANTS TO OOMYCETES, INFLUENCED BY AN ANTIBODIES Fusion - Google Patents
RESISTANCE OF PLANTS TO OOMYCETES, INFLUENCED BY AN ANTIBODIES Fusion Download PDFInfo
- Publication number
- RU2011133912A RU2011133912A RU2011133912/10A RU2011133912A RU2011133912A RU 2011133912 A RU2011133912 A RU 2011133912A RU 2011133912/10 A RU2011133912/10 A RU 2011133912/10A RU 2011133912 A RU2011133912 A RU 2011133912A RU 2011133912 A RU2011133912 A RU 2011133912A
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- Prior art keywords
- fusion protein
- antibody
- fragment
- plant
- phytophthora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43577—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies
- C07K14/43581—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from flies from Drosophila
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/14—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Insects & Arthropods (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
1. Белок слияния, содержащий по меньшей мере один противооомицетный белок или пептид (ПОБ), связанный с антителом или фрагментом антитела, специфически распознающий эпитоп Oomycota.2. Белок слияния по п.1, где ПОБ выбран из группы, состоящей из Сес, D4E1, GR7, Mag и мечниковина (МТК).3. Белок слияния по п.1 или 2, где антитело или его фрагмент специфически распознает эпитоп Phytophthora ssp., Pythium ssp., Peronospora ssp.и Pseudoperonospora ssp., предпочтительно, Phytophthora infestans, Phytophthora capsici, Phytophthora cactorum, Phytophthora cannamoni или Phytophthora nicotianae.4. Белок слияния по п.1, где антитело или его фрагмент представляет собой F(ab′)2 фрагмент, Fab фрагмент, scFv, биспецифический scFv, триспецифический scFv, диатело, однодоменное антитело (dAb), миниантитело или единицу молекулярного распознавания (ЕМР).5. Белок слияния по п.4, где фрагмент антитела представляет собой scFv, имеющий последовательность, выбранную из группы, состоящей из SEQ ID N0:61 - SEQ ID NO:69.6. Белок слияния по п.1, дополнительно содержащий по меньшей мере одну N-концевую и/или С-концевую метку, по меньшей мере облегчающую обнаружение и/или очистку белка слияния.7. Белок слияния по п.6, где метка выбрана из группы, состоящей из c-myc, his, his, tag54, FLAG, НА, HSV-, T7, S, стрептококковой и Е-метки.8. Белок слияния по п.1, дополнительно содержащий клеточную нацеливающую последовательность.9. Белок слияния по п.8, где клеточная нацеливающая последовательность представляет собой последовательность для выделения или локализации белка слияния в клеточных отделах или органеллах, предпочтительно, в апопласте, вакуоли, внутренних и/или внешних мембранах или просвете эндоплазматического ретикулума (ЭР).10. Белок слияния по п.1, где ПОБ и антитело или его фрагмент связаны пептидным1. A fusion protein containing at least one anti-oomycete protein or peptide (PHB) associated with an antibody or antibody fragment that specifically recognizes the Oomycota epitope. 2. The fusion protein according to claim 1, wherein the PHB is selected from the group consisting of Cec, D4E1, GR7, Mag, and Swordsman (MTK). The fusion protein according to claim 1 or 2, wherein the antibody or fragment thereof specifically recognizes the epitope of Phytophthora ssp., Pythium ssp., Peronospora ssp. And Pseudoperonospora ssp., Preferably Phytophthora infestans, Phytophthora capsici, Phytophthora cactorthnam phththumthophthnamthoma, Phytophthora cactorumthymthum. four. The fusion protein according to claim 1, wherein the antibody or fragment thereof is an F (ab ′) 2 fragment, a Fab fragment, scFv, a bispecific scFv, a trispecific scFv, a diatel, a single domain antibody (dAb), a mini-antibody, or a molecular recognition unit (EMP). 5. The fusion protein of claim 4, wherein the antibody fragment is scFv having a sequence selected from the group consisting of SEQ ID N0: 61 - SEQ ID NO: 69.6. The fusion protein of claim 1, further comprising at least one N-terminal and / or C-terminal tag, at least facilitating the detection and / or purification of the fusion protein. The fusion protein according to claim 6, wherein the label is selected from the group consisting of c-myc, his, his, tag54, FLAG, HA, HSV-, T7, S, streptococcal and E-labels. The fusion protein of claim 1, further comprising a cell targeting sequence. The fusion protein of claim 8, wherein the cell targeting sequence is a sequence for isolating or localizing the fusion protein in cell divisions or organelles, preferably in the apoplast, vacuole, internal and / or external membranes or lumen of the endoplasmic reticulum (ER). 10. The fusion protein of claim 1, wherein the PHB and the antibody or fragment thereof are linked by a peptide
Claims (22)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09168451 | 2009-08-24 | ||
EP09168451.4 | 2009-08-24 | ||
PCT/EP2010/061427 WO2011023522A1 (en) | 2009-08-24 | 2010-08-05 | Antibody fusion-mediated plant resistance against oomycota |
Publications (1)
Publication Number | Publication Date |
---|---|
RU2011133912A true RU2011133912A (en) | 2013-10-10 |
Family
ID=42829976
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2011133912/10A RU2011133912A (en) | 2009-08-24 | 2010-08-05 | RESISTANCE OF PLANTS TO OOMYCETES, INFLUENCED BY AN ANTIBODIES Fusion |
Country Status (8)
Country | Link |
---|---|
US (1) | US20120042416A1 (en) |
EP (1) | EP2478104A1 (en) |
AR (1) | AR077952A1 (en) |
AU (1) | AU2010288743A1 (en) |
BR (1) | BRPI1012085A2 (en) |
CA (1) | CA2754024A1 (en) |
RU (1) | RU2011133912A (en) |
WO (1) | WO2011023522A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2014273418B2 (en) * | 2013-04-29 | 2020-04-23 | Biotalys NV | Agrochemical compositions comprising antibodies binding to sphingolipids |
US10858666B2 (en) | 2014-11-05 | 2020-12-08 | Biotalys | Transgenic plants expressing a variable domain of a heavy chain antibody (VHH) that binds to a sphingolipid of a fungus |
EP3790586A4 (en) * | 2018-05-08 | 2022-01-19 | Phanes Therapeutics, Inc. | Anti-dll3 antibodies and uses thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10506274A (en) * | 1994-09-19 | 1998-06-23 | リークスラントボウユニフエルジタイト・ワゲニンゲン | Gene constructs encoding crop protection agents, and transformed plants containing and expressing such constructs, and methods of regulating disease organisms and pathogens in crops |
CA2345903C (en) | 1998-10-16 | 2006-09-26 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Molecular pathogenicide mediated plant disease resistance |
WO2003089475A2 (en) | 2002-04-22 | 2003-10-30 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Antibodies, recombinant antibodies, recombinant antibody fragments and fusions mediated plant disease resistance against fungi |
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2010
- 2010-08-05 AU AU2010288743A patent/AU2010288743A1/en not_active Abandoned
- 2010-08-05 CA CA2754024A patent/CA2754024A1/en not_active Abandoned
- 2010-08-05 BR BRPI1012085A patent/BRPI1012085A2/en not_active IP Right Cessation
- 2010-08-05 RU RU2011133912/10A patent/RU2011133912A/en unknown
- 2010-08-05 US US13/264,224 patent/US20120042416A1/en not_active Abandoned
- 2010-08-05 EP EP10740638A patent/EP2478104A1/en not_active Withdrawn
- 2010-08-05 WO PCT/EP2010/061427 patent/WO2011023522A1/en active Application Filing
- 2010-08-24 AR ARP100103089A patent/AR077952A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2010288743A1 (en) | 2011-09-01 |
CA2754024A1 (en) | 2011-03-03 |
EP2478104A1 (en) | 2012-07-25 |
AR077952A1 (en) | 2011-10-05 |
BRPI1012085A2 (en) | 2019-09-24 |
WO2011023522A1 (en) | 2011-03-03 |
US20120042416A1 (en) | 2012-02-16 |
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